脱钙骨

摘要： 目的 脱钙程度是影响脱钙骨基质物理性能的主要因素,完全脱钙的骨组织质地柔软,力学传导性不好,使用时易发生修补处的塌陷.故研究不同脱钙率的脱钙骨物理性能,为优选关节软骨支架材料提供理论基础.方法 通过原子吸收分光光度计检测同种异体骨的钙含量,然后将骨块浸泡于脱钙液中,实时检测脱钙液中的钙离子,根据脱钙液中的钙离子含量,控制脱钙率.分别检测脱钙率为0％、30％、50％、70％、80％、99.9％时同种异体骨的孔隙率、吸水率、孔径、力学性能和降解率.结果 随着脱钙率的增加,同种异体骨的孔径、孔隙率、吸水率、降解率均明显增加,钙的流失,使骨块的强度降低、韧性增加,与关节软骨的匹配性增强.结论 80％脱钙率条件下,同种异体骨块的性能最优,在保证拥有合适的孔径、孔隙率的前提下,还具有一定的机械强度,其降解速率与成骨速率也相当,是一种非常有前景的关节软骨修复支架材料.

摘要： Objective To investigate the effect of exogenous vascular endothelial growth factor (VEGF) on bone activity of rabbit heterotopic allograft decalcified bone.Methods 140 adult healthy China white rabbits were selected,no limitation with sex,20 rabbits as the donor preparation of allogenic decalcified bone,according to the random number table,the rest was divided into the experimental group (allograft decalcified bone ± VEGF) and the control group (Allograft decalcified bone),each group contained 60 rabbits.For the experimental group,the prepared 1.5 cm long homologous decalcified tibia was placed in rabbit right thigh of rectus femoris and vastus medialis muscle gap near by saphenous artery,and fixed on the femur with two 0.8 mm Kirschner wire.In the vicinity of the skin,implanted an osmotic pump which contain the VEGF solution 200 μl with concentration was 0.5 μg/ml.In the control group,implanted the isometric allograft decalcified bone in rabbit right thigh corresponding parts with the same method.Each group respectively at 0,2,4,6,8,10 weeks to death 10 white rabbits,By specimen observation,HE dyeing observation and detection of type Ⅰ glue protein fluorescence intensity,Analysis the bone activation degree of two groups of bone allograft decalcified.Results Experimental allograft decalcified bone gradually wrapped by connective tissue membrane,its surface appear different size of the pits and gradually increased and become deep,while the control group pits relatively little and shallow.In the experimental group and control group,the fluorescence intensity of type Ⅰ collagen reached its peak respectively at 8 weeks (47.57 ±3.50) and 10 weeks (45.07±6.02),with no statistically significant (P ＞ 0.05).Conclusion Rabbit allograft decalcified bone implanted in the muscle clearance with abundant blood supply can be transformed into activated bone after 10 weeks,and after applying exogenous VEGF,allograft decalcified bone can be transformed into activated bone after 8 weeks,the bone activation process obviously speed up.The reaults confirmed the exogenous VEGF can obviously promote the ectopic rabbit bone allograft decalcified bone activation process.%目的 探讨外源性血管内皮生长因子(VEGF)对异位兔同种异体脱钙骨骨活性的影响.方法 选用成年中国白兔140只,雌雄不限,取20只作为供体制备同种异体脱钙骨,其余按随机数字表分为实验组(同种异体脱钙骨加VEGF组)60只、对照组(同种异体脱钙骨组)60只.实验组将制备好的1.5 cm长同种异体脱钙胫骨段置于兔右大腿股直肌与股内侧肌间隙近隐动脉处,并用2根0.8 mm克氏针将其固定于股骨上,于其附近皮下植入一枚胶囊渗透压泵,泵内载有浓度为0.5 μg/ml的VEGF溶液200μl.对照组取等长同种异体脱钙骨同法置于兔右大腿相应部位.每组分别于术后0、2、4、8、10、12周处死10只白兔,通过大体标本观察、HE染色观察及I型胶蛋白荧光强度检测,分析判断两组同种异体脱钙骨骨活化程度.结果 实验组同种异体脱钙骨逐渐被结缔组织膜包裹,其表面出现大小不同的陷窝,并逐渐增多且加深,对照组陷窝相对少而浅.实验组和对照组的Ⅰ型胶原蛋白荧光强度分别在术后8周(47.57±3.50)、10周(45.07±6.02)达到峰值,两者差异无统计学意义(P＞0.05).结论 兔同种异体脱钙骨异位于血运丰富的肌间隙内经过10周可转变为活化骨,而施加外源性VEGF后,同种异体脱钙骨8周便可转变为活化骨,骨活化进程明显加快.证实了外源性VEGF可明显促进异位兔同种异体脱钙骨骨活化进程.

摘要： 背景：脂肪干细胞具有来源广泛、含量丰富、容易获取，培养条件简单且扩增能力强等优点，有可能成为继骨髓间充质干细胞之后最具前景的骨组织工程种子细胞。　　目的：探讨兔脂肪干细胞与脱钙骨支架材料复合修复尺骨缺损的可行性。　　方法：构建兔尺骨缺损动物模型，将单纯脱钙骨材料植入右侧缺损区，设为对照组，将成骨诱导后的兔脂肪干细胞-脱钙骨支架材料复合物植入左侧缺损区，设为实验组。植入后12周，获取骨缺损处组织标本进行CT扫描和组织学检测。　　结果与结论：经 CT 扫描，实验组骨缺损断端与材料的接合部位已不能清晰分辨，断端外侧可观察到平行骨痂。对照组脱钙骨未完全降解，可观察到清晰的骨断端，未观察到连续骨痂，骨缺损处大多为纤维连接，未出现骨性修复。经组织学检测，实验组缺损处为典型再生骨组织，存在骨细胞和骨陷窝以及骨小梁结构，骨细胞和骨陷窝数量较多，仅有部分骨小梁结构形成，并有少量胶原样结构穿插于再生骨组织之间；对照组有脱钙骨基质残留现象，并存在部分胶原纤维样组织，在边缘可观察到一定的骨膜成骨反应，但程度较轻，且未出现大片再生骨样组织。以上结果表明兔脂肪干细胞成骨诱导后与脱钙骨支架材料复合可以用于修复尺骨缺损。%BACKGROUND:Adipose-derived stem cel s have a wide variety of sources and strong proliferation ability, which are easy to access and simple to culture. Therefore, adipose-derived stem cel s that are secondary to bone marrow mesenchymal stem cel s are expected to become the most promising seed cel s for bone tissue engineering. OBJECTIVE:To explore the feasibility of rabbit adipose-derived stem cel s with demineralized bone matrix to repair ulna defects. METHODS:Ulna defect model was made in rabbits. Demineralized bone matrix was implanted into the right defect region as control group. After osteogenic induction, rabbit adipose-derived stem cel s/demineralized bone matrix composite was implanted into the left defect region as experimental group. At 12 weeks after implantation, defect tissues were taken for CT scanning and histological detection. RESULTS AND CONCLUSION:CT results showed that there was unclear boundary between the broken ends of fractured bone and the composite material in the experimental group, and paral el cal uses out of the broken end could be seen. In the control group, the broken end was clearly seen and no cal us occurred continuously. Fibers were connected at the defect site, and no new bone occurred. Histological findings showed that typical regenerated bone tissues were seen in the experimental group with osteocytes, bone lacunae and bone trabeculae;there were more osteocytes and bone lacunae, but bone trabecula was only seen in a part of bone defects;a few of col agens interlarded the regenerated bone tissues. In the control group, the residual of demineralized bone matrix was seen as wel as some col agenous fibers, and periosteal bone formed a little, but no large amount of regenerated osteoid tissues were found. These findings indicate that under osteogenic induction, rabbit adipose-derived stem cel s combined with demineralized bone matrix are feasible to repair ulna defects.

摘要： 背景：脂肪干细胞具有来源广泛、含量丰富、容易获取，培养条件简单且扩增能力强等优点，有可能成为继骨髓间充质干细胞之后最具前景的骨组织工程种子细胞。 目的：探讨兔脂肪干细胞与脱钙骨支架材料复合修复尺骨缺损的可行性。 方法：构建兔尺骨缺损动物模型，将单纯脱钙骨材料植入右侧缺损区，设为对照组，将成骨诱导后的兔脂肪干细胞-脱钙骨支架材料复合物植入左侧缺损区，设为实验组。植入后12周，获取骨缺损处组织标本进行CT扫描和组织学检测。 结果与结论：经 CT 扫描，实验组骨缺损断端与材料的接合部位已不能清晰分辨，断端外侧可观察到平行骨痂。对照组脱钙骨未完全降解，可观察到清晰的骨断端，未观察到连续骨痂，骨缺损处大多为纤维连接，未出现骨性修复。经组织学检测，实验组缺损处为典型再生骨组织，存在骨细胞和骨陷窝以及骨小梁结构，骨细胞和骨陷窝数量较多，仅有部分骨小梁结构形成，并有少量胶原样结构穿插于再生骨组织之间；对照组有脱钙骨基质残留现象，并存在部分胶原纤维样组织，在边缘可观察到一定的骨膜成骨反应，但程度较轻，且未出现大片再生骨样组织。以上结果表明兔脂肪干细胞成骨诱导后与脱钙骨支架材料复合可以用于修复尺骨缺损。

摘要： 背景：在骨缺损修复过程中，从修复质量、免疫排斥和疾病传播等多方面来衡量，自体骨都是最佳的选择，但来源有限且取骨区可能产生并发症，给骨缺损的修补及自体骨移植临床应用带来了很大局限。目的：以含自体骨髓间充质干细胞脱钙骨载体复合支架材料植入骨缺损的同时，向植入处微环境内添加碱性成纤维细胞生长因子等因素，从而达到增强骨修复能力，改进修复效果的目的。方法：选择3月龄新西兰大耳白兔45只，建立双侧前臂桡骨中下段骨-骨膜缺损模型，然后将实验兔等分为3组：实验组、对照组和空白组，均于左侧髂骨和股骨转子处抽取骨髓，分离培养扩增骨髓间充质干细胞后，与不同材料体外复合，植入兔桡骨干10 mm缺损处。实验组兔缺损处植入骨髓间充质干细胞、脱钙骨、藻酸钙、碱性成纤维细胞生长因子、维生素 C；对照组兔缺损处植入骨髓间充质干细胞、脱钙骨、藻酸钙；空白组兔双侧缺损处均不植入任何材料，自然愈合。结果与结论：植入后30，60，90 d各组之间组织学检查新骨生成速度、生成量差异均有显著性意义。实验组兔缺损修复部位骨痂和移植物化骨及材料降解明显快于对照组和空白组；但对照组和空白组兔缺损修复部位残存物明显多于实验组。实验组兔骨缺损以多点方式直接成骨，对照组和空白组则从两端以“爬行替代”方式成骨。空白组兔自然愈合后90 d骨缺损均无愈合。说明植入体外培养移植物的同时向该植入微环境添加碱性成纤维细胞生长因子和维生素C等有利骨修复，提高骨损伤的愈合。%BACKGROUND:Autogenous bone is the best choice for the repair of bone defects from the aspects of repair quality, immune rejection and disease transmission, but the limited sources and complications after bone colection bring a big limitation for bone defect repair and clinical application of autogenous bone graft. OBJECTIVE:To repair the bone defects with demineralized bone carrying autologous bone marrow mesenchymal stem cels and meanwhile to inject basic fibroblast growth factor and other factors into the micro-environment at the implant site, in order to enhance bone repair capacity and improve the repair effect. METHODS:Forty-five New Zealand white rabbits at an age of 3 months were selected to establish bone defect models (the lower segment of bilateral forearm bone-periosteum), and then, the animals were divided into three groups: experimental, control and blank groups. Bone marrow samples were extracted from the left iliac bone and femoral trochanter. Bone marrow mesenchymal stem cels were isolated, cultured and amplified folowed by co-cultured with different materials in vitro, and then, the composite scaffolds were implanted to the bone defects of the radial shaft. In the experimental group, bone marrow mesenchymal stem cels, demineralized bone, calcium alginate, basic fibroblast growth factor, vitamin C were implanted; in the control group, bone marrow n mesenchymal stem cels, demineralized bone, calcium alginate were implanted; in the blank group, nothing was implanted. RESULTS AND CONCLUSION:At 30, 60, 90 days after implantation, there were significant differences in the new bone formation rate and new bone amount between different groups. The bone formation speed and material degradation speed were significantly faster in the experimental group than the control and blank groups, while the amount of remnants at the defect region was larger in the latter two groups. Multi-point bone formation was visible in the experimental group, while “creeping substitution” was found in the control and blank group. At 90 days after implantation, bone defects were not healed in the blank group undergoing natural healing. These findings indicate that co-transplantation of bone graft and basic fibroblast growth factor and vitamin C is beneficial to bone repair and improves bone defect healing.

摘要： 背景:在骨缺损修复过程中,从修复质量、免疫排斥和疾病传播等多方面来衡量,自体骨都是最佳的选择,但来源有限且取骨区可能产生并发症,给骨缺损的修补及自体骨移植临床应用带来了很大局限。目的:以含自体骨髓间充质干细胞脱钙骨载体复合支架材料植入骨缺损的同时,向植入处微环境内添加碱性成纤维细胞生长因子等因素,从而达到增强骨修复能力,改进修复效果的目的。方法:选择3月龄新西兰大耳白兔45只,建立双侧前臂桡骨中下段骨-骨膜缺损模型,然后将实验兔等分为3组:实验组、对照组和空白组,均于左侧髂骨和股骨转子处抽取骨髓,分离培养扩增骨髓间充质干细胞后,与不同材料体外复合,植入兔桡骨干10 mm缺损处。实验组兔缺损处植入骨髓间充质干细胞、脱钙骨、藻酸钙、碱性成纤维细胞生长因子、维生素C;对照组兔缺损处植入骨髓间充质干细胞、脱钙骨、藻酸钙;空白组兔双侧缺损处均不植入任何材料,自然愈合。结果与结论:植入后30,60,90 d各组之间组织学检查新骨生成速度、生成量差异均有显著性意义。实验组兔缺损修复部位骨痂和移植物化骨及材料降解明显快于对照组和空白组;但对照组和空白组兔缺损修复部位残存物明显多于实验组。实验组兔骨缺损以多点方式直接成骨,对照组和空白组则从两端以"爬行替代"方式成骨。空白组兔自然愈合后90 d骨缺损均无愈合。说明植入体外培养移植物的同时向该植入微环境添加碱性成纤维细胞生长因子和维生素C等有利骨修复,提高骨损伤的愈合。

摘要： 目的探索骨粉和脱钙液比例和脱钙时间对脱钙骨基质（demineralized bone matrix,DBM）诱导成骨活性的影响。方法以深低温冷冻的供体骨为原材料,剔除软组织、脱脂、清洗、干燥、粉碎等过程制备骨粉;调整脱钙时间以及骨粉和脱钙液的比例,利用0.5 mol/L盐酸动态脱钙;清洗、冷冻干燥、辐照灭菌,得到DBM样品。对样品进行p H值和钙含量测定,从影像学观察显影效果,对其成骨活性进行体内骨诱导验证。结果测得DBM-15-2 h、DBM-20-2 h、DBM-25-2 h、DBM-20-0.5 h、DBM-20-1 h、DBM-20-1.5 h的钙含量依次为1.86±0.84、0.60±0.22、0.42±0.12、6.84±0.45、2.90±0.32、1.59±0.26;影像学结果显示DBM-20-0.5 h材料的显影密度相对最高;裸鼠体内骨诱导组织学观察发现,不同参数制备的DBM样品均具有骨诱导活性,且差异无统计学意义（P〉0.05）。结论骨粉和脱钙液比例为1 g/20 ml,脱钙0.5 h,即可制得具有稳定的骨诱导活性与一定显影能力的DBM。

摘要： Objective To prepare demineralized bone matrix ( DBM ) with a stable calcium content and could be displayed under X-ray by accurate control of the decalcification process.Methods Raw materials were from the donor bone stored in cryopreservation. Hydrochloric acid was used as decalciifcation solution. Soft tissue removal, degreasing, cleaning, drying, grinding and other processes were applied in the preparation of bone powder. Decalciifcation time as well as decalciifcation solution ratio were adjusted, and 0.5 mol / L hydrochloric acid dynamic decalciifcation was performed. DBM sample was obtained after cleaning, freeze drying and irradiation sterilization. The pH value, calcium content and imaging under X-ray were measured. Osteoinductivity was evaluated by implantation test in nude mice.Results The content of calcium of the DBM-15-2 h, DBM-20-2 h, DBM-25-2 h, DBM-20-0.5 h, DBM-20-1 h and DBM-20-1.5 h were 1.86±0.84, 0.60±0.22, 0.42±0.12, 6.84±0.45, 2.90±0.32 and 1.59±0.26, respectively. The brightness of the DBM-20-0.5 h sample was the highest under X-ray. The bone induction activity was seen in 4 weeks. There were no signiifcant differences in osteoinductivity in 6 samples (P>0.05 ).Conclusions A stable, radiopaque and osteoinductive DBM can be obtained when bone powder and decalciifcation solution ratio is 1 g / 20 ml ( decalciifed 0.5 h ).%目的探索骨粉和脱钙液比例和脱钙时间对脱钙骨基质( demineralized bone matrix，DBM )诱导成骨活性的影响。方法以深低温冷冻的供体骨为原材料，剔除软组织、脱脂、清洗、干燥、粉碎等过程制备骨粉；调整脱钙时间以及骨粉和脱钙液的比例，利用0.5 mol / L 盐酸动态脱钙；清洗、冷冻干燥、辐照灭菌，得到 DBM 样品。对样品进行 pH 值和钙含量测定，从影像学观察显影效果，对其成骨活性进行体内骨诱导验证。结果测得 DBM-15-2 h、DBM-20-2 h、DBM-25-2 h、DBM-20-0.5 h、DBM-20-1 h、DBM-20-1.5 h 的钙含量依次为1.86±0.84、0.60±0.22、0.42±0.12、6.84±0.45、2.90±0.32、1.59±0.26；影像学结果显示 DBM-20-0.5 h 材料的显影密度相对最高；裸鼠体内骨诱导组织学观察发现，不同参数制备的 DBM 样品均具有骨诱导活性，且差异无统计学意义(P＞0.05)。结论骨粉和脱钙液比例为1 g /20 ml，脱钙0.5 h，即可制得具有稳定的骨诱导活性与一定显影能力的 DBM。

摘要： Objective To construct the tissue engineering bone in vitro by implanting the rabit bone marrow stromal cells ( rBMSCs) , which were infected by a recombinant adenoviral vector carrying human BMP-2 and EGFP gene ( Ad-hBMP-2/EGFP) , into DBM.Methods The rBMSCs were isolated and cultured in vitro.The cell membrane markers were detected using flow cytometry.After transfection, the optimal multiplicity of infection ( MOI) of BMSCs was observed using fluorescence microscopy.The expression of exogenous gene in the cells was detected using Western blotting.The allogenic DBM was prepared according to the method described by Urist.After co-cultured with DBM in vitro, the adhesion rate of BMSCs on DBM was tested using cytometry.Then the transfected BMSCs were seeded on DBM.The fluorescence microscopy was used to observe whether the BMSCs successfully adhered to the DBM.And the attachment and growth of the cells on the scaffold were examined using SEM.Results The phenotype identification of BMSCs showed that the expression of D44 was positive, while the expression of CD45 was negative.After transfection, the expression of hBMP-2 increased, which was confirmed by Western blotting.The average adhesive rate of BMSCs on DBM was 72 .74%±1.99%.SEM examination revealed extensive cellular attachment and growth on the DBM.Conclusion The culture and identification of rBMSCs are successful.The rBMSCs are successfully transfected with Ad-EGFP-BMP2, with high efficiency expression of objective gene.The growth of the transfected cells seeded on DBM is good, indicating the successful construction of tissue engineering bone.%目的：用腺病毒载体介导人骨形态发生蛋白－2及EGFP基因转染兔骨髓基质干细胞（ rBMSC），种植DBM （脱钙骨）支架体外构建组织工程骨。方法兔髓基质干细胞（ rBMSC）的分离、培养；流式细胞仪检测细胞表面标记；转染后，荧光显微镜观察细胞最适感染复数（MOI）及蛋白印迹检测外源基因的表达情况；采用Urist提供的方法制备脱钙骨（DBM），用细胞计量法测定BMSCs与DBM复合培养的黏附率。然后将转染后细胞接种到DBM支架上，用荧光显微镜观察细胞是否成功粘附于支架材料上，扫描电镜观察细胞贴附、生长状况。结果 BMSCs细胞表型鉴定：CD44表达阳性，CD45表达阴性；转染后，蛋白印迹试验检测到BMP－2表达增高；骨髓间充质干细胞与脱钙骨基质的平均粘附率为（72．74±1．99）％；扫描电镜见转染细胞生长良好，伸出丝状突起，相互连接。结论成功培养及鉴定兔BMSCs，Ad－BMP－2／EGFP可高效转染兔BMSCs，转染后的细胞种植于DBM支架材料后生长状况良好，组织工程骨构建成功。

摘要： 目的：研究在选择性细胞滞留(selective cell retention,SCR)技术下,用纳米自组装肽RADA16-Ⅰ修饰脱钙骨基质(Demineralized Bone Matrix,DBM),然后富集构建自体骨髓复合物(SAP/DBM),验证修复重建羊股骨临界骨缺损的效果. 方法：通过纳米肽RADA16-Ⅰ的自组装特性修饰脱钙骨基质,形成复合支架SAP/DBM,体外通过激光共聚焦显微镜观察此复合支架对羊骨髓间充质干细胞(mesenchymal stem cells,MSCs)的形态变化,通过定量聚合酶链反应(PCR)检测成骨基因的表达水平.利用选择性细胞滞留技术,将骨髓中利于成骨的细胞及生长因子富集于SAP/DBM,形成复合物,体内通过影像学和组织学评价此复合物对羊股骨中段2cm临界骨缺损的修复重建效果. 结果：自组装肽可在DBM的大网孔内形成三维纳米网状纤维.MSCs在SAP/DBM复合材料上增殖更快,细胞形态铺展更好,相关成骨基因如碱性磷酸酶(ALP)、骨钙素(OCN)及成骨转录因子2(Runx2)的表达显著高于DBM对照组(P＜0.05).体内的成骨评价中,对比富集有自体骨髓的DBM材料,SAP/DBM复合物在羊骨缺损修复区形成的新生骨骨量显著提高(P＜0.05). 结论：本研究不仅为组织工程骨的构建提供了一种新的思路,而且还为临床临界骨缺损的修复重建提供了一种快速、简便、高效的方法.

摘要： 本发明提供了一种组织工程化移植物，它包括：(a)组织工程载体，所述的组织工程载体是脱细胞、脱钙的骨；(b)种子细胞。本发明还提供了该组织工程化移植物的制备方法和用途。

摘要： 本实用新型公开了一种应用于颅骨修复的同种异体骨表面脱钙皮质骨块，使用时嵌入待修补位置，利用连接片与宿主体骨缘固定，皮质块的填充位是絮状碳纤维组成，填充位将皮质块与宿主骨之间的间隙全部填满，而且不会对宿主骨产生额外压力，皮质骨上还设置有流道，将注液口中注入的促进剂均匀分散到填充位中，以提高成骨细胞活性，缩短愈合时间。

摘要： 本实用新型涉及脱钙装置技术领域，特别涉及骨穿刺组织自动脱钙脱水一体装置，包括液缸、支架、移动装置和放置盒；所述液缸上具有顺序设置的多个储液缸，多个的所述储液缸包括顺序设置的固定部分、脱钙部分、空白部分和脱水部分；所述支架与所述移动装置连接并通过所述移动装置带动所述支架沿所述储液缸的顺序方向移动；所述放置盒设置于所述支架上。本实用新型结构简单，可有效提高骨穿刺组织脱钙脱水效率以及可实现脱钙脱水过程自动化、规范化和标准化。

摘要： 本实用新型公开了一种用于检测骨标本脱钙程度的手持探测针，设置了包括针筒体、针座和探测针；所述针筒体的一端设有带盲孔的连接头，所述盲孔内设有内螺纹，所述针座一端设有六方表面并与探测针固定连接，针座另一端通过螺纹与连接头的盲孔连接。其有益技术效果是：探测针连接手柄，增加了操作安全性。针体可拆卸，手柄可长久使用。探测针体用钢笔帽式的筒盖帽覆盖，针筒体内部空腔内存放不同型号的探测针，使用更加方便。具有提高手感灵敏度，缩短检测时间，提高检测效率，携带方便，操作安全，提高医护操作效率的特点。

摘要： 本发明实施例公开了一种具有促骨生长和抗菌双功能的脱钙骨基质材料，在脱钙骨基质表面通过共价键引入聚合物，所述聚合物的侧链通过促骨生长信号分子和季铵盐进行修饰。本发明首先通过原子转移自由基反应得到聚合物，然后在聚合物侧链上引入促进骨生长信号分子和季铵盐，具有附着量多，附着率高的特点，所得到的聚合物进一步通过共价键的方式与脱钙骨基质进行复合，能够有效地将具有促骨生长功能的促骨生长信号分子和具有抗菌功能的季铵盐牢固的负载到脱钙骨基质表明，明显提高了脱钙骨基质的促骨活性，同时还具有杀菌的功能，该材料可以应用于骨缺损的修复及重建，应用前景广阔。

摘要： 本发明涉及一种利用一步法制备复合脱钙骨组合物的制备方法。根据本发明的复合脱钙骨组合物通过生物体源骨制备得到。此外，通过本发明制备的复合脱钙骨组合物中，按照骨原有比例含有骨无机质，可以提供最接近于体内骨形成环境的无机质含量条件。另外，含有骨形成蛋白质(BMP‑2)，从而可以带来稳定且优异的骨形成效果。

摘要： 本发明公开了一种用于骨缺损修复的异体脱钙骨泥及其制备方法，由异体脱钙骨基质颗粒50份，骨胶原冻干颗粒5～50份，无菌生理盐水10～200份或骨形态发生蛋白溶液10～200份组成；异体脱钙骨基质颗粒直径420～710微米，骨胶原冻干颗粒是经醛类交联剂或EDC碳化二亚氨类化合物类交联剂交联后的骨胶原冻干颗粒，骨形态发生蛋白溶液是含0.1～1mg/ml骨形态发生蛋白的氯化钙与盐酸胍的混合液。将三种组分混合制备的异体脱钙骨泥，具有可以任意塑型且塑型准确、良好的骨诱导和骨传导作用，无毒副作用，可广泛应用于骨科、口腔科、脑外科、整形外科、五官科等领域。

摘要： 本发明公开了一种天然组织来源的脱细胞联合脱钙骨材料的制备方法，该法将哺乳动物任意骨组织经含蛋白酶抑制剂的生理盐水缓冲液、有机溶剂溶液、含Triton？X的PBS缓冲液、含SDS的PBS缓冲液、含胰酶的PBS缓冲液、含DNA酶的PBS缓冲液、EDTA等渗液和超声波处理，获得脱细胞、脱钙的骨材料。本发明能同时对松质骨和密质骨进行完全去细胞化处理，且条件温和、无ECM损害、快速稳定，所得脱细胞联合脱钙骨材料具有生物相容性好、可塑性强、生物力学强度高等优点，可用于修复临床上各类病因造成的骨缺损和骨不愈合等骨再生、修复障碍。

摘要： 本发明公开了一种复合富血小板血浆和脱钙骨基质的骨修复材料及其制备方法，属于医疗材料领域。本发明的骨修复材料为含富血小板血浆和脱钙骨基质的凝胶，其制备方法包括以下步骤：(1)将脱钙骨基质粉末加入获取的富血小板血浆中，充分混合搅拌，制成混悬液；(2)将含Ca2+和凝血酶的溶液与步骤(1)中的混悬液按一定体积比混匀，静置，形成凝胶，得到骨组织修复材料。本发明利用自体富血小板血浆复合脱钙骨基质形成的人工骨材料，两者相辅相成，共同促进骨修复。富血小板血浆复合脱钙骨基质形成的凝胶可塑性强，可通过透视定位微创置入，也可通过开放手术植入，操作方便。

摘要： 本实用新型公开了一种应用于胸骨修复的脱钙骨条，包括外骨条、内骨条、固定部分，外骨条通过医用钢丝固定在胸骨外，减轻钢丝对胸骨的应力，内骨条安装在胸骨腔中，对胸骨腔进行支撑，并利用与胸骨腔的压力进行压迫止血，提高手术效果，同时外骨条还是用于成骨细胞附着并生长的场所，外骨条上的促生槽中填充有促生剂，促进成骨细胞的附着与生长，固定部分将外骨条与肋骨进行固定，固定部分不是一体成型的，而是由多个被连接绳连接的固定环组成，因此不仅能够牢固固定在肋骨上，同时不受肋骨弯曲形状的影响；本实用新型结构简单、人体亲和度高，能够促进成骨细胞增殖，愈合效果更好。