骨髓祖代细胞

摘要： 目的探讨清除巨噬细胞对小鼠造血干祖细胞(hematopoietic stem/progenitor cells,HSPC)归巢过程的影响。方法免疫磁珠分选获得小鼠HSPC,使用羧基荧光素二醋酸盐琥珀酰亚胺酯(Carboxyfluorescein diacetate succinimide ester,CSFE)染色标记,采用氯膦酸二钠脂质体(clodronate liposomes,Clod)方法建立去除巨噬细胞的小鼠模型,通过流式细胞术检测HSPC移植的归巢效率,通过实时荧光定量逆转录酶链式反应方法和酶联免疫吸附测定方法检测去除巨噬细胞后骨髓微环境中CXCL12、SCF和人血管内皮细胞粘附分子1(vascular adhesion molecule 1,VCAM1)分子mRNA表达水平和蛋白表达水平的变化。结果氯膦酸二钠脂质体作用后第24小时和第48小时,小鼠骨髓中CD11b+F4/80+巨噬细胞数量显著减少(P<0.05);移植小鼠HSPC后,相较于对照组,去除巨噬细胞组小鼠HSPC归巢效率显著降低(P<0.05);同时去除巨噬细胞组小鼠骨髓微环境中CXCL12、SCF的mRNA和蛋白表达量比对照组显著降低(P<0.05),而VCAM1表达量无显著变化。结论去除巨噬细胞能够显著降低小鼠HSPC归巢效率,该作用可能与去除巨噬细胞后导致的骨髓微环境中CXCL12与SCF含量减少有关。

摘要： 目的 探讨骨髓间充质干细胞(MSCs)减轻单侧输尿管梗阻(UUO)模型肾脏损伤的效果及相关的机制.方法 通过体外培养的方法制备MSCs,并将MSCs通过尾静脉注射的方法注入到UUO模型鼠体内,在造模的第14天检测不同组肾脏病理的改变、管周毛细血管、细胞凋亡及β-catenin表达情况来观察MSCs是否可以减轻肾脏损伤并探讨相关机制.结果 将培养的MSCs通过尾静脉注射的方法注入到UUO模型BALB/C小鼠体内,发现在MSCs组肾脏病理纤维化的程度较 UUO组明显减轻(HE:1.60±0.35比3.34±0.23;MASSON:21.32±7.54比51.08±4.45),同时MSCs注入可以进一步减少管周毛细血管的丢失(13.56±4.65比60.16±10.24)、抑制细胞的凋亡(14.32±3.54比28.16±6.21)及 β-catenin 表达(29.33±6.45比39.51±8.42).结论 MSCs治疗对慢性肾脏纤维化起到保护作用,作用机制与减少管周毛细血管丢失、抑制细胞凋亡及β-catenin的表达有关.

摘要： 近年来,越来越多的研究表明骨髓来源抑制性细胞(MDSC)参与了多种慢性肝病的发生发展,包括慢性病毒性肝炎、酒精性肝病、非酒精性脂肪性肝病、自身免疫性肝病、肝癌等.作为一种来源于骨髓祖细胞和未成熟髓系细胞的细胞群体,MDSC通过调节炎症反应、免疫细胞的分化及功能等在肝病的形成、进展和修复中起着关键作用.现就MDSC与肝脏各类型疾病之间联系的研究进展作一综述,以期为临床诊断、预后或治疗各类慢性肝病提供新思路.

摘要： 目的 探讨神经生长因子(nerve growth factor,NGF)对2型糖尿病小鼠骨髓基质细胞(bone marrow stromal cell,BMSC)增殖、成骨向分化及矿化能力的影响,为NGF的临床应用提供依据.方法 选择8周龄雄性2型糖尿病db/db小鼠3只和正常C57BL/6J小鼠2只,全骨髓贴壁法分离培养股骨BMSC.用糖尿病小鼠BMSC(糖尿病组)和正常小鼠BMSC(正常组)进行BMSC成骨能力比较实验(实验一);用糖尿病小鼠BMSC进行NGF对糖尿病小鼠BMSC成骨能力影响实验(实验二),分为糖尿病对照组、NGF组和K252a+NGF组[K252a为NGF高亲和力受体酪氨酸激酶A(tyrosinekinase,TrkA)的特异性阻断剂];K252a+NGF组加K252a 30 min后加NGF;NGF组仅加NGF,糖尿病对照组两者均不添加.甲基噻唑基四唑法检测实验一基础培养1、3、5、7d和实验二基础培养1、2、3d的细胞增殖情况;成骨诱导3、5、7d后检测碱性磷酸酶(alkaline phosphatase,ALP)水平;成骨诱导14d后茜素红染色观察矿化结节形成情况.每项实验每组均设置5个复孔,所有实验重复3次.结果 基础培养3、5、7d,糖尿病组BMSC增殖能力均显著高于相同时间点正常组(P＜0.05);成骨诱导3、5、7d,糖尿病组ALP水平均显著低于相同时间点正常组(JP＜0.05);成骨诱导14 d,糖尿病组矿化结节数量[(23.1±6.4)个/视野]显著少于正常组[(36.9±7.9)个/视野](P＜0.05).基础培养1、2、3d,糖尿病对照组、NGF组和K252a+NGF组BMSC增殖差异无统计学意义(P＞0.05);成骨诱导3、5d,NGF组ALP水平均显著高于糖尿病对照组(P＜0.05);成骨诱导3、5、7d,K252a+NGF组ALP水平均显著低于NGF组(P＜0.05)和糖尿病对照组(P＜0.05);成骨诱导14d,NGF组矿化结节数量[(45.2±6.8)个/视野]显著多于糖尿病对照组[(23.1±6.4)个/视野](P＜0.05),K252a+NGF组矿化结节数量[(18.0±4.5)个/视野]显著少于NGF组(P＜0.05)和糖尿病对照组(P＜0.05).结论 2型糖尿病小鼠BMSC体外成骨向分化及矿化能力降低.NGF通过与TrkA受体结合促进糖尿病小鼠BMSC体外成骨向分化,增强其矿化能力.%Objective To study the effects of nerve growth factor (NGF) on the proliferation,osteogenic differentiation and mineralization of type 2 diabetic mice bone marrow stromal cell (BMSC),providing basis for clinical application of NGF.Methods Three 8-week-old male db/db mice and two 8-week-old male C57BL/6J mice were used in the study.BMSC derived from femur were cultured though adherence method.BMSC of C57BL/6J mice and db/db mice was divided into normal group and diabetic group to conduct the osteogenic potential experiment,named experiment one.In experiment two,diabetic BMSC was divided into 3 groups:diabetic control group,NGF group,and K252a+NGF group [K252a was the inhibitor of tyrosine kinase A (TrkA),which was the high affinity receptor of NGF],to investigate effect of NGF on osteogenic potential of diabetic mice BMSC.After seeding BMSC,K252a was added into K252a+ NGF group,then NGF was added 30 min later.NGF was added into NGF group and K252a+NGF group,but not diabetic control group.The proliferation of BMSC at 1,3,5 and 7 d in experiment one and the proliferation of BMSC at 1,2 and 3 d in experiment two were evaluated through methyl thiazolyl tetrazolium,and the level of alkaline phosphatase (ALP) at 3,5 and 7 d in both experiments were measured.After being osteogenic induced for 14 d,mineralized nodules in both experiments were quantitated by alizarin red calcium stain.Five holes were set in every group,and all experiments were repeated 3 times.Results The BMSC proliferation of diabetic group was significantly higher than that of the normal group at 3,5 and 7 d (P＜0.05).After being osteogenic inducted for 3,5 and 7 d,ALP level of diabetic group were significantly lower than that of normal group (P＜0.05).After being osteogenic inducted for 14 d,calcium nodule count of diabetic group [(23.1±6.4) nodule/field] were significantly lower than that of normal group [(36.9±7.9) nodule/field](P＜0.05).At 1,2 and 3 d,BMSC proliferations of diabetic control group,NGF group and K252a+NGF group were not statistically different (P＞0.05).After being osteogenic inducted for 3 and 5 d,ALP level of NGF group was significantly higher than that of diabetic control group (P＜0.05).After being osteogenic inducted for 3,5,and 7 d,ALP level of K252a+NGF group was significantly lower than that of NGF group (P＜0.05) and diabetic control group (P＜0.05).After being osteogenic induced for 14 d,calcium nodule count of NGF group [(45.2±6.8) nodule/field] was significantly more than that of diabetic control group [(23.1±6.4) nodule/field] (P＜0.05);while calcium nodule count of K252a + NGF group [(18.0 ± 4.5) nodule/field] was significantly less than that of NGF group (P＜0.05) and diabetic control group (P＜0.05).Conclusions The differentiation and mineralization of type 2 diabetic mice BMSC was significantly reduced.NGF promoted the osteoblastic differentiation and mineralization of diabetic mice BMSC in viro though combining with TrkA.

摘要： 目的 探讨小鼠腹腔及骨髓源性高纯度肥大细胞体外诱导培养方法,并鉴定其功能.方法 分别从小鼠腹腔灌洗液及股骨获得腹腔及骨髓细胞,用白细胞介素3和干细胞因子分别联合诱导培养2周及4周,光镜下观察细胞形态,甲苯胺蓝染色鉴定肥大细胞成熟度,流式细胞仪检测细胞表面分子CD117和FceR Ⅰ α的表达情况,不同浓度Compound48/80刺激后镜下计数肥大细胞脱颗粒率,分光光度法测定肥大细胞β己糖胺酶释放率.结果 分别诱导培养2周及4周后,小鼠腹腔及骨髓细胞呈大小均一且具折光性的悬浮细胞.甲苯胺蓝染色示上述两种细胞胞质内含紫红色的异染颗粒.两种细胞表面CD117及FcεR Ⅰ α单阳性率均高于95％,双阳性率分别为97.68％±0.80％及96.12％±0.76％.100和1 000 mg/L Compound48/80刺激组与空白对照组肥大细胞相比,脱颗粒率差异有统计学意义(均P＜ 0.01).100 mg/L Compound48/80组骨髓源性肥大细胞以及10 mg/L、100 mg/LCompound48/80组腹腔源性肥大细胞β己糖胺酶释放率均较空白对照组显著升高(P＜ 0.01或0.05).结论 白细胞介素3和干细胞因子联合可诱导小鼠骨髓干细胞及腹腔细胞定向分化增殖,从而获得高纯度成熟且具有脱颗粒功能的肥大细胞,为后续细胞生物学研究奠定基础.%Objective To explore the in vitro culture methods for oriented differentiation of peritoneal cells and bone marrow cells into high-purity mast cells,and to identify the function of these mast cells.Methods Peritoneal cells and bone marrow cells were isolated from the peritoneal cavity lavages and femur of C57BL/6 mice,and cultured with both interleukin-3 (IL-3) and stem cell factor for 2 and 4 weeks respectively.Light microscopy was performed to observe the morphology of these cells,toluidine blue staining to identify the degree of maturity of these mast cells,and flow cytometry to measure the expression of cell surface markers C D 117 and FceR Ⅰ α.After the stimulation with compound 48/80 at different concentrations,the degranulation rate of mast cells was counted under the microscope,and β-hexosaminidase release rate was measured by spectrophotometry.Results After 2-or 4-week culture,the mouse peritoneal and bone marrow cells all manifested as refractive suspension cells of uniform size.Toluidine blue staining showed violaceous metachromatic granules in the cytoplasm of the two kinds of cells.The proportions of CD117 or FcεR Ⅰ α single-positive peritoneal and bone marrow-derived mast cells were all more than 95％,and the proportions of CD117/FcεR Ⅰ α double-positive peritoneal and bone marrow-derived mast cells were 97.68％ ± 0.80％ and 96.12％ ± 0.76％ respectively.The degranulation rates of mast cells in the 100-and 1 000-mg/L compound 48/80 groups significantly differed from those in the blank control group (all P ＜ 0.01).Compared with the blank control group,the β-hexosaminidase release rates significantly increased in bone marrow-derived mast cells in the 100-mg/L compound 48/80 group and peritoneal mast cells in the 10-and 100-mg/L compound 48/80 groups (P ＜ 0.01 or 0.05).Conclusion IL-3 and stem cell factor can co-induce the directed differentiation and proliferation of mouse bone marrow stem cells and peritoneal cells,so as to harvest highnuritv mature degranulated mast cells,and lay a foundation for subsequent cell biology research.

摘要： 目的：研究吲哚-3-甲醇（I3C）对小鼠骨髓造血细胞辐射损伤的保护作用及机制。方法（1）密度梯度离心法获得CD45.1亚型C57BL/6J小鼠骨髓有核细胞，经0 mol/L、10-8 mol/L~10-3 mol/L I3C处理后，接受不同剂量（0 Gy、1 Gy、4 Gy）的137Csγ-射线照射；继续培养18 h后采用生物发光法检测细胞活力。（2）设空白对照组和10-6 mol/L I3C组，经上述3种剂量射线照射后，接种于甲基纤维素半固体培养基中培养7 d，观察骨髓粒-单核巨噬细胞集落（CFU-GM）形成情况。（3）取24只CD45.2亚型小鼠接受8 Gy 137Csγ-射线照射作为受体，CD45.1亚型小鼠骨髓有核细胞（供体）设空白对照组、4 Gy照射组和4 Gy照射+10-6 mol/L I3C组。将供体与竞争者（CD45.2亚型）骨髓细胞混和后，接种于受体小鼠体内（每组8只），流式细胞术检测受体小鼠外周血细胞中供体来源的细胞比例。（4）细胞设空白对照组、10-6 mol/L I3C组、1 Gy照射组和1 Gy照射+10-6 mol/L I3C组。培养24 h后收集细胞，提取蛋白后Western blot法检测各组核因子NF-E2相关因子（Nrf2）、血红素加氧酶（HO）-1表达。结果（1）I3C浓度>10-4 mol/L时出现明显的细胞毒性作用（P>0.05）；相同剂量射线照射下，10-7~10-6 mol/L I3C可减轻射线对细胞的损伤；因此选取10-6 mol/L为本研究I3C的实验浓度。（2）相同剂量射线照射下，10-6 mol/L I3C组CFU-GM形成数量较空白对照组明显升高（P<0.05）。（3）流式细胞结果显示，4 Gy照射组细胞移植后，受体小鼠外周血中供体细胞比例较对照组明显降低（P<0.05），而10-6 mol/L I3C预处理的供体小鼠细胞移植后，受体小鼠中供体细胞比例较4 Gy照射组升高（P<0.05）。（4）Western blot结果显示，1 Gy照射+10-6 mol/L I3C组Nrf2和HO-1蛋白表达水平明显高于其他3组（P<0.05）。结论 I3C可以减轻辐射引起的小鼠造血细胞损伤和功能下降，其机制可能与激活Nrf2/HO-1通路有关。%Objective To investigate the protective effect of indole-3-carbinol (I3C) on radiation-induced mouse bone marrow hematopoietic cell injury and the involved mechanisms. Methods (1) The bone marrow nuclear cells (BMNCs) from CD45.1 subtype of C57BL/6J mice were collected by a density gradient centrifugation method. The BMNCs were pretreated with a series doses of I3C (0 mol/L, 10-8 mol/L-10-3 mol/L) and then exposed with radiation of 137Csγ-ray (doses of irradiation were 0 Gy, 1 Gy and 4 Gy). After 18-hour culturing, the bioluminescence method was used to detect the cell viability. (2) These cells were divided into control group and 10-6 mol/L I3C group. Both groups were received the irradiation (0 Gy, 1 Gy and 4 Gy) and inoculated into the methylcellulose semi-solid culture medium to incubate 7 days, the colony forming unit-granulocyte monocytes (CFU-GM) were observed. (3) Twenty-four CD45.2 subtype mice used as the receptor were exposed with 8 Gy radiation. The CD45.1 BMNCs were divided into control group, 4 Gy irradiation group, 4 Gy irradiation and 10-6 mol/L I3C group. Donor cells were harvested from C57BL/6J (CD45.1) mice after they received various treatments, and were then mixed with competitive BMNCs from C57BL/6J (CD45.2) mice. The mixed cells were transplanted into recipient mice (8 mice/group). Flow cytometry was used to analyze the proportion of donor cells in peripheral blood of receptor. (4) The cells were divided into control group, 10-6 mol/L I3C group, 1 Gy irradiation group, 1 Gy irradiation with 10-6 mol/L I3C group. After 24-hour culturing, Western blot assay was used to detect the expression levels of nuclear factor erythroid 2-related factor 2 (Nrf2) and hemeoxygenase-1 (HO-1). Results (1) I3C showed a significant cytotoxic effect on the BMNCs when its concentration was above 10-4 mol/L. 10-7-10-6 mol/L I3C could reduce the radiation injury of BMNCs under the same dose of irradiation. Therefore, 10-6 mol/L I3C was chosen for subsequent experiments. (2) The CFU-GM was significantly higher in 10-6 mol/L I3C group than that of control group (P<0.05). (3) Results of flow cytometry showed that the proportion of donor cells in receptor was significantly higher in 4 Gy irradiation group than that of control group, which decreased the engraftment capability of irradiated HSCs (P<0.05), although the engraftment capability of irradiated HSCs improved after 10-6 mol/L I3C treatment. (4) I3C significantly enhanced the increased protein expression of Nrf2 and HO-1 caused by radiation (P<0.05). Conclusion I3C has a protective effect on hematopoietic cells following radiation-induced injury, which may be related with activating the Nrf2/HO-1 signal pathway.

摘要： 背景：如何使移植的骨髓干细胞更多的在肝内停留、分化是一个重要问题,也是经肝动脉途径移植骨髓干细胞治疗肝硬化的关键。目的：探讨经肝动脉途径移植自体骨髓干细胞对肝硬化的治疗效果。方法：将30只新西兰大白兔随机分为3组：正常对照组,干细胞移植组,模型对照组,每组10只。正常对照组不做任何处理,其余2组均建立肝硬化模型,干细胞移植组经肝动脉途径给予自体骨髓干细胞。在骨髓干细胞移植后的第1,2,4,8,10周分别检测肝功能指标,在第10周进行肝脏病理学检查。结果与结论：（1）骨髓干细胞移植第10周,干细胞移植组血清谷丙转氨酶、总胆红素、谷草转氨酶水平有所降低,活化部分凝血激酶时间明显减短,白蛋白水平升高,与模型对照组比较,差异有显著性意义（P〈0.05）;（2）干细胞移植组肝细胞形态完整,没有明显的水肿,假小叶结构仍然存在,与模型对照组比较,肝组织的纤维化程度明显减轻;（3）结果表明,经肝动脉途径移植自体骨髓干细胞可在短时间内提高体内白蛋白含量,改善机体肝功能,对肝硬化具有一定的治疗作用。

摘要： 目的比较阳离子聚合物Xfect与PDS-1000基因枪系统转染人骨髓间充质干细胞（hBMSCs）的转染效率及两种方法对细胞增殖能力的影响。方法应用贴壁法原代培养hBMSCs,取第3代细胞以流式细胞术鉴定细胞表面抗原CD29、CD34、CD45、CD90,并分别用Xfect与PDS-1000基因枪系统将pcDNA-胰岛素样生长因子1（IGF-1）质粒转导入hBMSCs中。转染后在不同时间点使用倒置荧光显微镜观察绿色荧光,计数阳性细胞,计算细胞转染率及细胞漂浮率;以酶联免疫吸附试验（ELISA）方法检测IGF-1分泌量。结果 hBMSCs培养成功后,高表达CD29、CD90,低表达CD34、CD45。在转染后1、2d,两种方法细胞转染率差异有统计学意义（t=4.734、5.324,P〈0.05）。两种方法转染后细胞IGF-1分泌量均较转染前增加,但两种方法间比较差异有统计学意义（t=2.220-10.678,P〈0.05）。两种方法转染均有部分细胞脱落。结论两种方法均可将pcDNA-IGF-1基因转染入hBMSCs内,转染后细胞能分泌IGF-1。Xfect转染试剂转染效率较高,PDS-1000基因枪系统转染效率较低,两者对细胞都造成一定损伤。

摘要： 目的探讨新型纳米活性组织工程支架复合骨髓间充质干细胞（BMSCs）修复兔大段桡骨缺损的血管化进程及骨缺损修复能力。方法将44只成年新西兰大耳白兔制备成双侧桡骨中段15mm的骨-骨膜缺损模型,随机选取4只白兔不植入任何材料作为空白组;剩余的40只白兔在其右侧桡骨缺损处植入复合BMSCs的新型纳米活性组织工程支架作为实验组,在其左侧植入新型纳米活性组织工程支架作为对照组。术后2、4、6、8周分批处死动物,行组织学观察,并检测各组血管化程度。结果组别和时间对血管化程度影响存在交互作用（F=78.137,P〈0.01）。对照组和实验组组内任意两个时间点血管化程度比较,差异均有统计学意义（F=79.225、7 803.609,P〈0.01）。组间两两比较显示,第2、4周,实验组的血管化程度高于对照组,第6周实验组的血管化程度低于对照组,差异有统计学意义（t=10.244～25.036,P〈0.01）;第8周两组血管化程度比较差异无显著性（P〉0.05）。结论新型纳米活性组织工程支架能够较快发生血管化,复合BMSCs的新型纳米活性组织工程支架成骨能力较单纯支架材料强。

摘要： Objective To review the research and progress of treating intervertebral disc degeneration ( IDD) by using BMSCs transplantation, and provide theoretical basis for further basic researches. Methods CNKI database and ISI Web of Knowledge database from 2000 to 2014 were retrieved by the first author with the key words of " bone marrow mesenchymal stem cells (BMSCs), cell therapy, intervertebral disc degeneration ( IDD )" in Chinese and in English, respectively. The biological characteristics and cultivation of BMSCs, the influence factors of BMSCs differentiate into intervertebral disc cells and cell therapy for IDD by BMSCs transplantation in vivo were summarized. Results We can obtain enough amount of BMSCs. BMSCs has low immunogenicity and good differentiation potential, and these make BMSCs a very good seed cell source for cell therapy of IDD. Under the condition of improving the micro environment of IDD, or a special support material system for cell cultivation, or the induction of cytokines, or co-cultured with intervertebral disc cell, BMSCs can differentiate into nucleus pulposus cells, express proteoglycan and collagen-Ⅱ. Whether autograft or allograft, even heterogeneous, BMSCs can survive and propagate in degenerative intervertebral disc for long-term, promote the secretion of intervertebral disc cells matrix. Conclusions As the study of BMSCs and cellular therapy, a lot of work has been done on treating IDD by using BMSCs transplantation and we have made some achievements. Although using BMSCs transplantation in the treatment of IDD also shows huge application prospect, there are still many problems need to be solved before applying it in clinical treatment.%目的：总结骨髓间充质干细胞(BMSCs)治疗椎间盘退变(IDD)的相关研究成果,为BMSCs 治疗 IDD 的临床应用提供理论基础。方法计算机检索 CNKI 数据库和 ISI Web of Knowledge数据库,限定时间为2000—2014年,中英文检索词分别为“骨髓间充质干细胞、细胞治疗、椎间盘退变”和“bone marrow mesenchymal stem cells ,cell therapy,intervertebral disc degeneration ”。从检索所得的论文中选择近期权威杂志上发表的与 BMSCs 治疗 IDD 密切相关的实验研究或临床研究,排除重复性研究、Meta 分析以及综述类文章,主要从 BMSCs 的分离培养和生物学特性、BMSCs 向椎间盘细胞分化的影响因素、BMSCs 体内移植治疗 IDD 的研究等方面对所纳入的文章进行分析总结。结果BMSCs 能取得足够量的细胞,具有较低的免疫原性和良好的分化潜能,以其作为 IDD 基因治疗靶细胞具有显著的优越性。在改善退变椎间盘内的微环境、一定的支架材料的培养体系、细胞因子的刺激诱导、与椎间盘细胞共培养等条件下,BMSCs 可以向髓核细胞分化,表达蛋白多糖和Ⅱ型胶原。无论是自体还是同种异体,甚至是异种 BMSCs 移植均可以在退变椎间盘内长期存活、增殖,促进椎间盘细胞基质分泌,从而有效缓解 IDD 进程。结论随着对 BMSCs 和细胞治疗的研究,目前对 BMSCs 移植治疗 IDD 已经进行了大量研究并取得了一定的成果,但是真正将其应用到临床上,仍然存在很多问题,需要进一步研究解决。

摘要： 本发明涉及一种能够促进骨髓有核细胞及骨髓基质细胞增殖的系列肽及其应用，它的生物活性超过现有的各种造血生长因子并解决了现有基因重组技术所存在的生产工序复杂以及产品效果不稳定的缺陷。本发明药物结构简单，可有效的应用于治疗再生障碍性贫血病人、癌症病人化疗后骨髓抑制等全血细胞减少症包括造血干细胞、祖细胞、粒细胞系、红细胞系、淋巴细胞系、巨核细胞等各类细胞减少症，以及一切基于骨髓细胞减少的病症，和治疗一切基于骨髓基质细胞减少的骨质疏松症，具有较大的应用推广价值。

摘要： 本发明公开了一种显微多光谱骨髓及外周血细胞自动分析仪，它包括光源、显微镜、显示器及、面阵CCD和计算机；其特征在于：a、有一个滤光系统；b、有一个CCD控制和数据采集系统；c、有一个三维移动控制系统；d、还有计算机。本发明还公开了一种分析骨髓及外周血细胞的方法，其工作程序包括下述步骤：a、自动聚焦；b、光谱图像的采集；c、基于光谱图像的细胞分割；d、图像特征测量；e、细胞分类；f、自动扫描；g、疾病分类。本发明显微多光谱骨髓及外周血细胞自动分析仪和分析骨髓及外周血细胞方法自动化程度高，分析结果准确，可靠性强，充分利用显微光谱对血细胞进行光谱识别和分类，为血液病的诊断提供新的光谱学依据。

摘要： 一种将基因转移到脂肪细胞或祖代脂肪细胞中的方法，包括在存在在分子中兼具逆转录病毒结合位点和目标细胞结合位点的物质、或存在具有逆转录病毒结合位点的物质与具有目标细胞结合位点的物质的混合物的情况下，用具有外源基因的逆转录病毒载体感染脂肪细胞或祖细胞，其中所述目标细胞结合位点具有能够结合VLA－5的区域和/或能够结合VLA－4的区域。

摘要： 本发明提供了一种用于悬浮培养骨髓细胞的培养基、制备方法、应用和诱导骨髓源细胞分化为巨噬细胞的方法，涉及细胞培养技术领域，本发明提供的用于悬浮培养骨髓细胞的培养基，通过各特定组分的相互配合，能够实现骨髓源细胞的全悬浮培养，获得高质量的全悬浮细胞，同时高效促进骨髓源细胞增殖分化为巨噬细胞。并且，该培养基成分明确，成本可控，通过将不同功能的组分独立包装，还具有使用方便的优点。

摘要： 本文描述了一种祖代B细胞刺激因子，它促进前-B细胞的形成。本文还公开了该因子的生产和纯化方法以及编码该因子的DNA的序列。该因子可以用于治疗造血性疾病和用于骨髓移植。

摘要： 本发明属于细胞培养技术领域，特别涉及一种骨髓间充质干细胞培养基及骨髓间充质干细胞培养方法。所述骨髓间充质干细胞培养基包括基础培养基、血清、庆大霉素、两性霉素B、维生素C、碱性成纤维细胞生长因子和地塞米松。所述基础培养基为DEME。所述血清为胎牛血清、人体血清中的任意一种。本发明提供的骨髓间充质干细胞培养基培养能够增强骨髓间充质干细胞的增殖能力和附着效率，同时不影响骨髓间充质干细胞的多功能性，有利于骨髓间充质干细胞的临床研究和应用。

摘要： 提供包含肉桂鞣质B1及五没食子酰基葡萄糖中的至少一种的骨髓干细胞的诱导剂及通过该诱导剂对骨髓干细胞进行诱导的骨髓干细胞的诱导方法。

摘要： 本发明涉及一种能够促进骨髓有核细胞及骨髓基质细胞增殖的系列肽及其应用，它的生物活性超过现有的各种造血生长因子并解决了现有基因重组技术所存在的生产工序复杂以及产品效果不稳定的缺陷。本发明药物结构简单，可有效的应用于治疗再生障碍性贫血病人、癌症病人化疗后骨髓抑制等全血细胞减少症包括造血干细胞、祖细胞、粒细胞系、红细胞系、淋巴细胞系、巨核细胞等各类细胞减少症，以及一切基于骨髓细胞减少的病症，和治疗一切基于骨髓基质细胞减少的骨质疏松症，具有较大的应用推广价值。

摘要： 本发明涉及表达对于能够将免疫细胞特异性和反应性重定向于表达CD123的细胞的重组嵌合蛋白的CD123特异性的嵌合抗原受体(CAR)的TCRKO‑或TCR KO和dCK KO‑工程化的免疫细胞，并且更具体地，其中胞外配体结合是源自CD123单克隆抗体的scFV，其赋予抗CD123阳性细胞的特异性免疫。赋予这种CD123 CAR的工程化免疫细胞特别适用于治疗复发难治性AML和母细胞性浆细胞样树突细胞肿瘤，并用作骨髓移植前的治疗。

摘要： 本发明公开了骨髓血管内皮细胞在骨髓增生异常综合征中的应用。本发明提供了骨髓血管内皮细胞作为标记物在如下任一中的应用：制备用于检测或者辅助检测MDS的病程进展情况的产品、制备用于诊断或者辅助诊断MDS的产品、区分或者辅助区分MDS和非MDS的产品。本发明研究发现从MDS‑MLD、MDS‑EB到AML患者，骨髓血管内皮细胞数量逐渐增加，但功能异常逐渐加重。此外，随着疾病进展，MDS患者的骨髓血管内皮细胞在体外对正常造血细胞的支持能力下降，对恶性造血细胞的支持能力升高。本发明对于检测MDS的发生发展，特别是监控MDS的病程进展具有重要意义。