摘要：
Objective To analyze the regulatory of miRNA (miR)-149-5p for the expression of Aurora-B in esophageal squamous cell carcinoma (ESCC). Methods The pathologic histology paraffin blocks of 61 patients with ESCC in Shanxi Provincial People's Hospital from January 2010 to December 2017 were collected. The immunohistochemical staining and tissue section in situ hybridization method were used to observe the expressions of Aurora-B and miR-149-5p in the tumor tissues and adjacent mucosas of the patients, and their relationship with clinicopathological parameters was analyzed. miRNA was predicted by using software TargetScan and miR walk. The relationship between Aurora-B and miR-149-5p were verified by using Western blot in ESCC TE-1 cells. Results The result of immunohistochemical staining showed that in 61 patients, 37 (61％) tumor tissues showed higher expression of Aurora-B compared with adjacent mucosas, the Aurora-B expression in 15 (26％) tumor tissues were in line with benign tissues, the Aurora-B expression in 9 (13％) tumor tissues were inferior to benign tissues. The expression of Aurora-B were not correlated with age (χ2=0.008, P=0.929), gender (χ2=0.088, P=0.767), grade of differentiation (χ2=2.632, P=0.268), but correlated with TNM staging (χ2=15.153, P<0.01) and lymph node metastasis (χ2=5.979, P=0.014). The miR-149-5p was predicted to combine with Aurora-B 3'untraslated region (UTR) by using TargetScan and miRwalk software. The result of in situ hybridization showed that the miR-149-5p showed low expression in 22 (36％) tumor tissues. The expression of miR-149-5p was correlated with Aurora-B expression (χ2 = 5.622,P= 0.018), and not correlated with age (χ2= 2.617, P= 0.106), gender (χ2= 1.529, P= 0.216), grade of differentiation (χ2 = 2.854, P= 0.240), TNM staging (χ2 = 0.870, P= 0.351) and lymph node metastasis (χ2= 0.128, P= 0.351). The Western blot results of TE-1 cells showed that the expression of Aurora-B in simultaneous over-expression of miR-149-5p and Aurora-B group was higher than that in over-expression of miR-149-5p group, and lower than that in over-expression of Aurora-B group. Conclusion The miR-149-5p can be involved in ESCC progression through regulating the expressions of Aurora-B.%目的 探讨食管鳞状细胞癌中miRNA-149-5p(miR-149-5p)对Aurora-B表达的调控作用.方法 收集山西省人民医院2010年1月至2017年12月收治的61例食管鳞状细胞癌患者的病理组织蜡块,免疫组织化学染色检测肿瘤组织以及对应癌旁组织Aurora-B表达,组织切片原位杂交检测miR-149-5p表达,分析二者与临床病理参数之间的关系.通过TargetScan和miR walk软件预测对应miR-149-5p结合位点.运用蛋白质印迹法验证食管鳞状细胞癌TE-1细胞中Aurora-B及miR-149-5p的关系.结果 免疫组织化学染色结果显示,61例患者中,37例(61％)肿瘤组织Aurora-B表达高于癌旁组织,15例(26％)与癌旁组织一致,9例(13％)低于癌旁组织;Aurora-B表达与患者年龄(χ2=0.008,P=0.929)、性别(χ2=0.088,P=0.767)、分化程度(χ2=2.632,P=0.268)无关,而与TNM分期(χ2=15.153,P<0.01)和淋巴结转移(χ2=5.979,P=0.014)有关.TargetScan和miRwalk软件预测发现,miR-149-5p与Aurora-B 3''非翻译区(UTR)结合.61例石蜡组织切片原位杂交结果显示,miR-149-5p在22例(36％)患者肿瘤组织中低表达,miR-149-5p表达与Aurora-B表达有关(χ2=5.622,P=0.018),与患者年龄(χ2=2.617,P=0.106)、性别(χ2=1.529,P=0.216)、分化程度(χ2=2.854,P=0.240)、TNM分期(χ2=0.870,P=0.351)和淋巴结转移(χ2=0.128,P=0.351)无关.蛋白质印迹法检测结果显示,TE-1细胞miR-149-5p和Aurora-B同时过表达组的Aurora-B表达高于miR-149-5p单独过表达组,低于Aurora-B单独过表达组.结论 miR-149-5p可能通过调控Aurora-B表达参与食管鳞状细胞癌发展进程.
