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胎血

胎血的相关文献在1988年到2021年内共计515篇,主要集中在基础医学、临床医学、妇产科学 等领域,其中期刊论文513篇、会议论文2篇、专利文献38512篇;相关期刊146种,包括中国病理生理杂志、国际检验医学杂志、医学临床研究等; 相关会议2种,包括全国免疫标记技术讨论会、全国首届超氧化物歧化酶学术会议等;胎血的相关文献由1798位作者贡献,包括侯怀水、时庆、沈柏均等。

胎血—发文量

期刊论文>

论文:513 占比:1.31%

会议论文>

论文:2 占比:0.01%

专利文献>

论文:38512 占比:98.68%

总计:39027篇

胎血—发文趋势图

胎血

-研究学者

  • 侯怀水
  • 时庆
  • 沈柏均
  • 鞠秀丽
  • 黄绍良
  • 吴燕峰
  • 魏菁
  • 李树浓
  • 方建培
  • 马艳萍
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 韩霞; 云升
    • 摘要: 目的 探讨人脐带血单个核细胞(hUCB-MNCs)联合人脐带间充质干细胞(hUC-MSCs)对乙型肝炎相关失代偿期肝硬化患者的肝功能、炎症程度及免疫功能的影响.方法 选取2016年11月—2019年6月在内蒙古医科大学附属医院诊治的11例肝硬化患者,所纳入患者第1周输注1次hUCB-MNCs(>18×109/次),第2、3、4周每周输注1次hUC-MSCs,每次输注1×106/kg,在治疗结束的第4、8、12周进行复查,对比治疗前后肝功能、血氨、凝血因子、血清细胞因子及淋巴细胞亚群的变化,同时观察记录神经、精神症状的改变.计量资料组间比较采用单因素重复测量方差分析.结果 hUCB-MNCs联合hUC-MSCs治疗后的11例患者其精神、神经症状与细胞输注前相比较有所改善;肝功能各项指标及凝血功能基本趋于正常(P值均<0.05);细胞联合输注后的第12周,血氨水平显著下降(P<0.05);炎性细胞因子IL-6、TNFα水平降低(F值分别为49.497、37.071,P值均<0.05);而抗炎细胞因子TGFβ和IL-10水平显著上升(F值分别为35.843、15.918,P值均<0.05);同时CD3+CD8+T淋巴细胞和CD19+B淋巴细胞均降低(F值分别为52.242、89.097,P值均<0.05);免疫调节性T淋巴细胞CD4+CD25+Treg明显升高(F=17.337,P<0.05).结论 hUCB-MNCs联合hUC-MSCs治疗肝硬化,与细胞输注前相比较,可改善肝功能,减少炎性细胞因子产生,减轻肝脏炎症反应以及肝细胞的破坏,升高免疫调节性T淋巴细胞,从而影响其自身免疫功能.
    • 万毅; 谢明月; 唐云; 张丽娇
    • 摘要: 目的 研究新生儿脐血细胞因子弹性蛋白酶特异性抑制物(Elafin)、白细胞介素18(IL-18)、γ-干扰素(IFN-γ)/白细胞介素4(IL-4)与乙型肝炎病毒(HBV)宫内感染的相关性.方法 选取2016年5月—2019年5月攀枝花市妇幼保健院收治的HBV携带产妇分娩的新生儿96例.其中,31例发生HBV宫内感染新生儿作为宫内感染组,65例未感染新生儿作为宫内未感染组.另选取同期该院非HBV携带且健康产妇分娩的新生儿90例作为对照组.比较3组脐血Elafin、IL-18、IFN-γ、IL-4水平及IFN-γ/IL-4,分析新生儿宫内感染影响因素、脐血因子之间关联性及与HBV-DNA载量相关性.结果 宫内感染组Elafin、IL-18、IFN-γ、IFN-γ/IL-4低于宫内未感染组、对照组,IL-4高于宫内未感染组、对照组(P<0.05).宫内感染组与宫内未感染者组产妇HBV大三阳、羊水情况、阴道流血史的比较,差异有统计学意义(P<0.05).产妇HBV大三阳[(O^R)=3.125(95%CI:2.379,4.105),P<0.05]、产妇羊水浑浊[(O^R)=2.781(95%CI:2.041,3.790),P<0.05]、产妇阴道流血史[(O^R)=2.389(95%CI:1.816,3.144),P<0.05]、Elafin[(O^R)=0.574(95%CI:0.405,0.813),P<0.05]、IL-18[(O^R)=0.529(95%CI:0.352,0.796),P<0.05]、IFN-γ/IL-4[(O^R)=0.475(95%CI:0.440,0.512),P<0.05]是新生儿HBV宫内感染的影响因素.Elafin与IL-18呈正相关(r=0.649,P<0.05),IFN-γ/IL-4与Elafin、IL-18呈正相关(r=0.529和0.499,P<0.05).HBV-DNA载量≥1.00×104copies/ml患者Elafin、IL-18、IFN-γ/IL-4水平较<1.00×104copies/ml患者低(P<0.05).Elafin、IL-18、IFN-γ/IL-4与HBV-DNA载量呈负相关(r=-0.601、-0.556和-0.712,均P<0.05).结论 Elafin、IL-18、IFN-γ/IL-4在新生儿宫内感染中呈异常表达,并与HBV-DNA载量密切相关.
    • 黄涛; 张莹; 黄娅芬; 李静; 方红育; 周少怀; 卞峰; 李宏亮; 任敏; 范明宇; 汪平; 谢西茜
    • 摘要: 目的 探讨人脐带间充质干细胞来源外泌体(huc-MSCs-exo)对骨关节炎(OA)大鼠软骨凋亡的影响.方法 取健康、足月剖宫产新生儿脐带,分离并提取huc-MSCs-exo,选取Wistar大鼠30只,随机分为假手术组、模型组及治疗组(各10只),模型组及治疗组制备OA模型大鼠,治疗组成模后关节腔内注射huc-MSCs-exo 100μg,治疗8周后;比较各组大鼠步态障碍评分、软骨组织形态学变化、软骨细胞凋亡情况及一氧化氮(NO)表达;Real-time PCR检测软骨组织肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β、IL-4、B细胞淋巴瘤基因-2(Bcl-2)、Bcl-2相关X蛋白(Bax)mRNA表达.另取12只大鼠,6只正常饲养、6只进行膝OA造模,模型成功后分离、培养OA软骨细胞并染色鉴定;正常软骨细胞为对照组,OA软骨细胞培养后分为OA组和实验组,实验组软骨细胞加入1 g/L huc-MSCs-exo 0.2 ml培养,对照组及OA组软骨细胞给予同体积磷酸盐缓冲液培养,3组细胞均培养24 h,LSD t检验比较各组细胞增殖抑制率、凋亡情况及NO表达.结果 治疗组与模型组比较,治疗组大鼠步态障碍评分降低(t=5.49,P<0.001),NO含量降低(t=15.69,P<0.001),凋亡指数(AI)值降低(t=7.42,P<0.001),TNF-α表达量降低(t=5.74,P<0.001),IL-1β表达量降低(t=17.22,P<0.001)、IL-4表达量降低(t=11.33,P<0.001),Bax mRNA表达量降低(t=19.51,P<0.001),Bcl-2 mRNA表达量升高(t=10.12,P<0.001),差异均具有统计学意义.结论 Huc-MSCs-exo可有效改善膝骨OA大鼠软骨细胞凋亡情况,其机制可能与改善炎症反应有关.
    • 苗宏健; 张磊; 赖建强; 孙亮; 赵云峰; 李敬光
    • 摘要: 目的 分析北京市新生儿脐带血中邻苯二甲酸酯代谢物含量与新生儿出生指标的关联.方法 于2015年2-7月,在北京市海淀区妇幼保健医院招募符合标准的待产孕妇及其新生儿为研究对象,共纳入161对孕妇及新生儿.问卷调查孕妇年龄、吸烟、饮酒等人口学信息,并在分娩后采集脐带血.采用超高效液相色谱-三重四极杆质谱仪测定脐带血邻苯二甲酸酯代谢物含量,并采用多元线性回归模型分析其与新生儿体重、身长和出生体重指数的关联.结果 161名孕妇年龄为(30.3±3.o)岁;161名婴儿体重为(3447.2±413.0)kg,身长为(50.2±l.l)cm,出生体重指数为(26.7±2.2)kg/m3,男婴占51.6%(83名).脐带血中检出的7种邻苯二甲酸酯代谢物:邻苯二甲酸甲酯、邻苯二甲酸异丁酯、邻苯二甲酸丁酯、邻苯二甲酸单-2-乙基己酯、邻苯二甲酸单(2-乙基-5-氧己基)酯、邻苯二甲酸单(2-乙基-5-羧基戊基)酯和邻苯二甲酸乙酯含量分别为(3.50±0.28)、(2.65±0.47)、(4.31±0.55)、(6.26±0.57)、(1.71±0.13)、(1.10±0.09)、(0.47±0.06)ng/ml.多元线性回归模型分析结果显示,7种邻苯二甲酸酯代谢物含量与新生儿体重、身长和出生体重指数均无关联(P值均>0.05).结论 北京市新生儿脐带血中邻苯二甲酸酯代谢物的含量较低,与新生儿体重、身长和出生体重指数无关联.
    • 卢婍; 余晋霞; 吕铖; 施蓉; 田英
    • 摘要: 目的 了解孕妇和新生儿脐血中铅、镉、砷、汞、锰和硒元素的含量,分析其胎盘通透性.方法 于2010年9月至2013年12月在山东省渤海莱州湾某二甲医院,招募符合标准的孕妇及其新生儿为研究对象.共纳入773对孕妇及其新生儿.根据所测定元素的种类,分为汞测定组(595对)、铅测定组(534对)和镉、砷、锰、硒测定组(244对).问卷调查获得孕妇及新生儿的人口学信息,检测母血和脐血中铅、镉、砷、汞、锰和硒的浓度,并计算各元素的胎盘通透性(某元素脐血浓度/母血浓度);采用Spearman秩相关分析母血和脐血中各元素的相关性.结果 773名母亲年龄为(28.34±4.50)岁,孕周为(39.47±1.39)周;773名新生儿出生体重为(3 419.47±497.39)g.母血和脐血中铅、镉、砷、汞、锰和硒各元素的中位数浓度分别为31.12和30.02、1.19和0.47、8.05和6.03、0.69和1.26、100.70和105.55、127.25和115.00μg/L.铅、镉、砷、汞、锰和硒的胎盘通透性分别为0.98、0.41、0.73、1.73、0.96和0.91.铅、镉、汞、锰和硒在母血和脐血中均呈正相关,Spearman相关系数分别为0.397、0.298、0.698、0.555和0.285(P值均<0.001).结论 铅、镉、砷、汞、锰和硒元素在母血和脐血中均普遍检出;汞的胎盘通透性较高.
    • 薛立军; 杜桂莲; 李思涛; 肖昕; 马飞
    • 摘要: Objective To study the predictive value of umbilical cord blood regulatory T cells (Treg) for bronchopulmonary dysplasia (BPD) in preterm infants. Method From June 2017 to December 2018, premature infants with gestational age less than 32 weeks admitted to NICU of our hospiatal were prospectively selected. The umbilical cord blood was collected at birth to examine the Treg amount. The infants were assigned into BPD group and non-BPD group according to the diagnosis at discharge. The differences of Treg amount between the two groups and different degrees of BPD were analysed. Result A total of 124 premature infants (GA<32 weeks) were admitted, including 41 cases in BPD group (mild, n=18;moderate, n=14; severe, n=9) and 83 cases in the non-BPD group. The BPD group had GA of (29.6 ± 1.1) weeks and birth weight (BW) of (1128 ± 135) g. The non-BPD group had GA of (29.8 ± 1.1) weeks and BW of (1316 ± 180) g. The birth weight, 1min and 5min Apgar scores in BPD group were lower than the non-BPD group (P<0.001). The BPD group had higher incidence of respiratory distress syndrome, longer duration of mechanical ventilation (MV) and oxygen inhalation(P<0.001) than the non-BPD group. The MV duration and oxygen inhalation duration in the severe BPD group were significantly longer than the moderate and mild BPD groups, and the duration in the moderate group was longer than the mild group (P<0.001). The number of Treg in cord blood in the BPD group [(1.43 ± 0.06) × 105 cells/ml] was significantly lower than the non-BPD group [(2.57 ± 0.09) × 105 cells/ml], and the difference was statistically significant (P<0.001). Multivariate Logistic regression analysis showed that a significant decrease in the number of Treg was a risk factor for BPD in premature infants (OR=0.000, 95%CI 0.000 ~ 0.012, P=0.009). The number of Treg in umbilical cord blood was negatively correlated with the severity of BPD. The area under the ROC curve showed that the cut-off value was 1.95 × 105 cells/ml, with Youden index 0.613, sensitivity 85.4% and specificity 75.9%. Conclusion The number of cord blood Treg cells may be a useful biomarker for predicting BPD in premature infants.%目的 探讨脐血调节性T细胞(regulatory T cells,Treg)检测对早产儿支气管肺发育不良(bronchopulmonary dysplasia,BPD)的预测价值.方法 前瞻性选择2017年6月至2018年12月深圳市宝安区松岗人民医院新生儿重症监护病房收治的胎龄<32周的早产儿,采集出生时脐血检测Treg数量,根据出院时是否发生BPD分为BPD组和非BPD组.分析两组患儿Treg数量及其在BPD不同程度间的差异.结果 最终纳入胎龄<32周的早产儿124例,其中BPD组41例(轻度18例、中度14例、重度9例),胎龄(29.6±1.1)周,出生体重(1128±135)g;非BPD组83例,胎龄(29.8±1.1)周,出生体重(1316±180)g.BPD组出生体重、1 min和5 min Apgar评分低于非BPD组、新生儿呼吸窘迫综合征发生率高于非BPD组、机械通气时间及吸氧时间长于非BPD组,差异有统计学意义(P<0.001).重度BPD组新生儿机械通气时间及吸氧时间明显长于中度组和轻度组,且中度组机械通气时间长于轻度组,差异均有统计学意义(P<0.001).BPD组脐血Treg数量明显低于非BPD组[(1.43±0.06)×105个/ml比(2.57±0.09)×105个/ml],差异有统计学意义(P<0.001).多因素Logistic回归分析显示,Treg数量减少是早产儿发生BPD的危险因素(OR=0.000,95%CI 0.000~0.012,P=0.009);脐血Treg数量与BPD严重程度成负相关.受试者工作特征曲线分析脐血Treg预测BPD的最佳临界值为1.95×105个/ml,敏感度85.4%,特异度75.9%.结论 脐血Treg检测可能对早产儿发生BPD具有早期预测作用.
    • Shi Wangtianyi; Yang Jun; Cai Yu; Wan Liping; Niu Jiahua; Wang Chun; Jiang Jieling
    • 摘要: 目的 评价亲缘单倍体异基因造血干细胞移植(Haplo-HSCT)联合第三方脐带血输注治疗高危淋巴细胞肿瘤的效果.方法 回顾性分析上海市第一人民医院2012年4月至2015年4月20例确诊高危淋巴细胞恶性肿瘤并接受Haplo-HSCT联合脐带血输注治疗患者的资料,与同期匹配资料接受非血缘异基因造血干细胞移植(MUD-HSCT)的15例患者以及接受同胞全相合异基因造血干细胞移植(MSD-HSCT)的14例患者资料进行对比,评价Haplo-HSCT联合脐带血输注治疗高危淋巴细胞肿瘤的效果.预处理方案以替尼泊苷、环磷酰胺、全身照射为主,移植物抗宿主病(GVHD)预防方案包括环孢素A联合短程甲氨蝶呤,所有Haplo-HSCT联合脐带血输注以及MUD-HSCT患者加用抗胸腺细胞球蛋白(ATG).结果 移植后MUD-HSCT组和MSD-HSCT组各有1例患者21 d内死亡,不能评价,其余患者成功植入. Haplo-HSCT+脐带血组、MUD-HSCT组和MSD-HSCT组中性粒细胞中位植入时间分别为13 d (10~18 d)、12 d(9~16 d)和12 d(9~14 d);血小板中位植入时间分别为11 d(9~18 d)、12 d(10~23 d)和12 d(9~14 d). Haplo-HSCT+脐带血组、MUD-HSCT组和MSD-HSCT组100 d发生Ⅱ~Ⅳ级急性GVHD分别有10、3、3例,2年发生慢性GVHD分别有6、4、3例;2年累积复发率分别为40.6%、66.2%和26.7%,预计2年总生存(OS)率分别为37.9%、42.9%和55.4%,以上各组数据比较差异均无统计学意义(均P>0.05).结论 Haplo-HSCT联合脐带血输注治疗高危淋巴细胞肿瘤与MUD-HSCT或MSD-HSCT疗效类似.在缺乏匹配供者的情况下,可推荐其用于高危淋巴细胞肿瘤的治疗.%Objective To evaluate the efficacy of haploidentical allogeneic hematopoietic stem cell transplantation (Haplo-HSCT) combined with third-party umbilical cord blood (UCB) infusion in treatment of high-risk lymphoblastic malignancies. Methods The clinical data of 20 patients with high-risk lymphoblastic malignancies who received Haplo-HSCT from April 2012 to April 2015 in Shanghai General Hospital were retrospectively analyzed, which were compared with the data from 15 patients who underwent matched unrelated donor HSCT (MUD-HSCT) or 14 matched sibling donor HSCT (MSD-HSCT) during the same period. The efficacy of Haplo-HSCT combined with UCB infusion in treatment of high-risk lymphoblastic malignancies was evaluated. The preparative regimen mainly consisted of teniposide, cyclophosphamide and total body irradiation (TBI). Graft versus host disease (GVHD) preparative regimen included cyclosporine and a short term of methotrexate. The patients who received Haplo-HSCT combined with UCB infusion and MUD-HSCT were treated with antithymocyte globulin (ATG). Results After the transplantation, one patient in MUD-HSCT group and one in MSD-HSCT group died within 21 days, and other patients were engrafted successfully. The median time of neutrophil engraftment was 13 days (10-18 d), 12 days (9-16 d) and 12 days (9-14 d) in Haplo-HSCT + UCB group, MUD-HSCT group and MSD-HSCT group, respectively; the median time of platelets engraftment was 11 days (9-18 d), 12 days (10-23 d) and 12 days (9-14 d), respectively. There were 10, 3, 3 cases of grade Ⅱ-Ⅳacute GVHD at day 100 in the three groups, respectively, and there were 6, 4, 3 cases of chronic GVHD in the three groups, respectively. The 2-year cumulative incidence of relapse was 40.6%, 66.2% and 26.7%, respectively. The predicted 2-year overall survival rate was 37.9%, 42.9% and 55.4%, respectively. All these data had no significant difference (all P> 0.05). Conclusion The efficacy of Haplo-HSCT combined with UCB infusion is similar to that of MUD-HSCT or MSD-HSCT in treatment of high-risk lymphoblastic malignancies, which should be recommended to the patients with high-risk lymphoblastic malignancies and without matched donors.
    • 刘岱纯; 徐锡金; 郑相斌; 蒋友胜; 张建清; 霍霞
    • 摘要: Objective To study the pollution status of polybrominated diphenyl ethers (PBDEs),polychlorinated biphenyls (PCB),polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) in cord blood of newborns in an e-waste dismantling area of Guangdong Province.Methods We recruited 20 eligible mothers and newborns who could meet the inclusion criteria in local hospitals of Guiyu in 2007.The inclusion criteria included directly engaged in dismantling e-waste during pregnancy and within 1 year before pregnancy;living in the e-waste dismantling workshops or the distance between living place and the e-waste dismantling areas was ≤200 m;the father of newborn was directly engaged in electronic waste dismantling for more than 1 year;the frequency of visiting the e-waste dismantling workshop during pregnancy was ≥3 times in a week.Questionnaires and physical examinations were performed on maternal and neonatal,and cord blood was collected from newborns to detect PCDD/Fs,PCB and PBDE.The concentration level of organic pollutants was corrected by the blood lipid content,and the total toxicity equivalent was calculated.The correlation between three compounds was analyzed by Spearman correlation.Results The mothers of the 20 newborns were (23.45±3.27) years old and lived for more than 5 years.The number of one parent engaged in e-waste dismantling,the mother or father smoking,and parent engaged in e-waste dismantling work were 3,13,15 and 19,respectively.The weight of newborns ranged from 2.5 to 3.6 kilogram and the Apgar score was 10 points.No adverse birth outcomes such as preterm birth,malformation or stillbirth were found.The median (maximum,minimum) concentration of PCBs,PCDD/Fs and PBDEs in cord blood were 263.22 (328.29,244.19),38.42 (147.49,12.68),39.33 (265.11,14.81) pg/g lipid,respectively.The median (maximum,minimum) of toxic equivalence concentrations of PCDD/Fs and PCB were 3.94 (9.24,2.69) and 15.95 (26.64,9.28) pg TEQ/g lipid.PBDE,the proportion of PBDE,PCB and PCDD/Fs in cord blood was 50.41%,49.25% and 0.34%,respectively.PCBs and PBDEs were positively correlated (r=0.733,P=0.039).Conclusion The high concentrations of PCDD/Fs,PCB,and PBDE were detected in the e-waste dismantling area.It is recommended that the risk of such substances on the health of local people should be assessed in a timely manner.%目的 了解广东省某电子垃圾拆解区新生儿脐带血中二噁英类化合物暴露水平.方法 2007年于广东省某电子垃圾拆解区当地医院招募符合纳入标准(怀孕期间及怀孕前1年内直接从事电子垃圾拆解者;在电子垃圾拆解作坊居住或居住场所距离电子拆解活动距离≤200 m;丈夫直接从事电子垃圾拆解1年以上者;怀孕期间前往电子垃圾拆解作坊的频数≥3次/周)的产妇及其新生儿各20名.对产妇进行问卷调查,对新生儿体格检查并采集新生儿脐带血,检测脐带血中的多氯代二苯并二噁英/呋喃(PCDD/Fs)、多氯联苯(PCB)和多溴联苯醚(PBDE)的浓度水平,采用血脂含量对测定结果进行校正,并计算总的毒性当量(TEQ);采用Spearman相关分析PCDD/Fs与PBDE、PCB与PBDE的相关性.结果 20名新生儿的母亲年龄为(23.45±3.27)岁,居住年限均超过5年;父母双方均至少有一方从事电子垃圾拆解;新生儿体重范围2.5~3.6 kg,未发现新生儿早产、畸形、死产等不良出生结局.脐血中的PCDD/Fs、PCBs和PBDEs总质量浓度中位数(最大值~最小值)分别为263.22(328.29~244.19)、38.42(147.49~12.68)和39.33(265.11~14.81)ng/g脂重;PCDD/Fs、PCB总毒性当量浓度中位数(最大值~最小值)分别为3.94(9.24~2.69)和15.95 (26.64~9.28) pg TEQ/g脂重.脐带血中PBDE、PCB和PCDD/Fs所占比例分别为50.41%、49.25%和0.34%.PCB和PBDE呈正相关(r=0.733,P=0.039).结论 电子垃圾拆解区新生儿脐带血中检测出二噁英类化合物PCDD/Fs、PCB和PBDE,该类化合物已在产妇体内形成蓄积,建议适时评估其对当地人群健康的影响.
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