摘要：
Objective To set up the models of human esophageal cancer in nude mice and the models of different xenograft tumors in IRM-2 mice,observe the effects of chemotherapy and radiotherapy on the tumors.Methods Twenty-four hours after setting up the models,all the mice carrying tumors including lymphoma,hepatic carcinoma H22,leukemia L1210 and sarcoma S180 were randomly divided into 3 groups:control group,radiotherapy group and cyclophosphamidum group,10 mice per group.Mice in cyclophosphamidum group were injected cyclophosphamidum (25 mg/kg) by intraperitoneal 4 times every other day;mice in radiotherapy group were given total body irradiation at the fourth day and last for 5 days.Mice with human esophageal cancer were randomly divided into 3 groups:control group,radiotherapy group and 5-fluorouracil group,5 mice per group.Mice in radiotherapy group were given local body irradiation at the fourth day and the eighth day.Mice in 5-fluorouracil group were injected 5-fluorouracil (25 mg/kg) by intraperitoneal 4 times every other day.All the mice were killed at the twelfth day and the rates of tumor inhibition were calculated.Results The tumor inhibitory rates of IRM-2 mice in radiotherapy group were 34.57％ (lymphoma),32.69％(sarcoma S180),31.31％(hepatic carcinoma H22) and 18.32％(leukemia L1210) respectively,and there were significant difference compared with control group(t=4.130,3.222,3.581 and 2.713,P＜0.01,＜0.01,＜0.01 and ＜0.05).The tumor inhibitory rates of nude mice with human esophageal cancer in radiotherapy group was 22.99％,but there was no significant difference compared with control group(t=1.235,P＞0.05).The tumor inhibitory rates in radiotherapy group were 74.47％(lymphoma),72.59％(sarcoma S180),69.12％(hepatic carcinoma H22),77.53％(leukemia L1210) and 56.32％(human esophageal cancer),and there were significant difference compared with control group(t =12.694,12.208,7.223,11.964 and 5.266,all P＜0.01).Conclusions There were inhibitory effects of chemotherapy and radiotherapy on all types of tumors.However,the efficiency depends on the mouse strains and the type of tumor.%目的 建立人食管癌及小鼠不同移植瘤模型,比较放、化疗对小鼠的不同肿瘤模型的抑制作用.方法 动物模型建立24 h后,将不同肿瘤细胞(淋巴瘤、肉瘤S180、肝癌H22、白血病L1210)的荷瘤IRM-2近交系小鼠随机分为对照组、照射组、环磷酰胺组,每组10只.环磷酰胺组于接种隔日腹腔注射环磷酰胺(25 mg/kg)1次,0.2 ml/只,共4次.照射组于接种后第4天进行全身1 Gy照射,每日1次,连续5d.将食管癌EC901荷瘤裸鼠随机分为对照组、照射组、5-氟尿嘧啶组,每组5只.照射组于接种后第4天和第8天对肿瘤局部进行2 Gy照射,5-氟尿嘧啶组于接种后隔日腹腔注射5-氟尿嘧啶(25 mg/kg)1次,0.2 ml/只,共4次.所有小鼠于第12天处死,解剖瘤块称重,计算抑瘤率.结果 裸鼠及IRM-2近交系小鼠皮下移植瘤模型的成瘤率均为100％,放疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210荷瘤小鼠的肿瘤有显著的抑制作用,抑瘤率分别为34.57％、32.69％、31.31％和18.32％,与对照组比较差异有统计学意义(t=4.130、3.222、3.581和2.713,P分别为＜0.01、＜0.01、＜0.01、＜0.05).放疗对食管癌EC901的抑瘤率为22.99％,但与对照组比较差异无统计学意义(t=1.235,P＞0.05).化疗对淋巴瘤、肉瘤S180、肝癌H22、白血病L1210、食管癌EC901荷瘤小鼠的抑瘤率分别为74.47％、72.59％、69.12％、77.53％和56.32％,与对照组比较差异有统计学意义(t=12.694、12.208、7.223、11.964和5.266,P均＜0.01).结论 放、化疗对小鼠的不同肿瘤模型均有抑制作用.由于小鼠的品系和瘤源不同,对放、化疗的敏感性也存在差异.
