摘要:
目的 探讨基于新型18F微反应器的18F-阿法肽(Alfatide II)的自动化制备方法,并行 荷瘤裸鼠micmPET/CT显像.方法 采用自主研制的18F微反应器和水相[18F]氟化铝螯合标记法, 自动化制备18F-Alfatide II,测量其放化产率,对18F-Alfatide II注射液进行质量分析.建立乳腺癌ZR-75-1 和鼻咽癌CNE1荷瘤裸鼠模型各3只,分别于注射18F-Alfatide II后0.5、1.0和2.0 h行micrePET/CT 显像,勾画感兴趣区(ROI),计算肿瘤/肌肉(T/M)比值.结果采用18F微反应器自动化制备了18F-Alfatide II,总合成时间约40min,放化产率为(28±6)%(非衰变校正,n=11),放化纯>97%,质量分析指标均符合要求.ZR-75-1和CNE1荷瘤裸鼠18F-Alfatide II microPET/CT显像清晰,肿瘤部位放射性摄取明显,注射后1.0 h T/M比值>4.0.结论 成功采用18F微反应器自动化制备18F-Matide II,合成时间短,放化产率高,有助于其在microPET/CT肿瘤显像中的研究.%Objective To develop the automated preparation of 18F-Alfatide II using newly-designed 18F-minireactor and perform 18F-Alfatide D microPET/CT imaging in tumor.Methods The automated preparation of 18F-Alfatide H was developed by using 18F-microreactor and water phase Al18F-chelating method,and the radiochemical yield and quality analysis were measured.The nude mice bearing breast tumor ZR-75-1 and nasopharyngeal tumor CNE1 were established(n = 3 respectively).MicroPET/CT imaging was performed at 0.5,1.0 and 2.0 h after the injection of 18F-Alfatide II.The region of interest(ROI)was depicted and the tumor/muscle(T/M)ratio was calculated.Results 18F-Alfatide II was automatically prepared with the total synthesis time of 40 min,the radiochemical yield of(28±6)%(no decay corrected,n=11),and the radiochemical purity >97%.All quality analysis indexes accorded with the radiopharmaceutical requirements.18F-Alfatide II microPET/CT images of ZR-75-1 and CNE1 tumors were clear due to the high radioactivity uptake of tumor lesions(T/M ratio was greater than 4.0 at 1.0 h after injection).Conclusion Based on the 18F-minireactor,the,8F-Alfatide II can be prepared successfully with short synthesis time and high radiochemical yield,which can help the application studies in 18F-Alfatide II microPET/CT imaging.