您现在的位置: 首页> 研究主题> 生物材料图片文章

生物材料图片文章

生物材料图片文章的相关文献在2013年到2013年内共计85篇,主要集中在基础医学 等领域,其中期刊论文85篇、专利文献958037篇;相关期刊1种,包括中国组织工程研究等; 生物材料图片文章的相关文献由205位作者贡献,包括张涛、于广栋、于美丽等。

生物材料图片文章—发文量

期刊论文>

论文:85 占比:0.01%

专利文献>

论文:958037 占比:99.99%

总计:958122篇

生物材料图片文章—发文趋势图

生物材料图片文章

-研究学者

  • 张涛
  • 于广栋
  • 于美丽
  • 何伟
  • 何晓峰
  • 侯喜君
  • 俞兴
  • 关春辉
  • 冉海琼
  • 冯伟

生物材料图片文章

-相关期刊

  • 期刊论文
  • 专利文献

搜索

排序:

学科

年份

    • 靳宪辉; 高脊光; 崔胜杰; 刘桂花; 杨建博; 林月秋
    • 摘要: 背景:异体肌腱移植是目前修复肌腱缺损的理想方法,但移植后的排斥反应使其使用受到限制.目的:观察碳化二亚胺交联改性脱细胞处理的版纳近交系微型猪肌腱移植修复兔跟腱缺损的效果.方法:横向切除40只日本大白兔双侧跟腱,随机分组:实验组以碳化二亚胺交联改性脱细胞版纳近交系微型猪肌腱移植修复,对照组以自体肌腱移植修复.结果与结论:①组织学观察:两组新生组织内细胞主要都是单核的成纤维细胞和纤维细胞,术后1-4周主要是呈椭圆形或圆形的成纤维细胞,细胞聚集区的细胞周围有新生胶原形成,这些胶原的走向较紊乱;局灶性条索状成熟胶原呈岛状分布,形成所谓“胶原岛”;术后12周时细胞越来越拉长,成为梭形和长条形的纤维细胞.②实验室检测:两组白细胞、C-反应蛋白水平、羟脯氨酸含量及抗拉强度差异均无显著性意义.说明碳化二亚胺交联改性的脱细胞版纳近交系微型猪肌腱能成功修复兔跟腱缺损,且具有组织相容性好、移植排斥反应轻、生物力学性能强的优点.
    • 李善昌; 龚立强; 杜晓岩
    • 摘要: 背景:各种原因导致的牙齿拔除后都会造成剩余牙槽嵴的吸收,导致颌骨骨量不足,不利于患者后期接受义齿和种植牙的修复治疗.目的:通过建立大鼠拔牙后剩余牙槽嵴模型,观察纳米晶胶原基骨/活性肽 P17-骨形态发生蛋白2对拔牙窝修复及牙槽嵴吸收的影响.方法:将36只健康 SD 大鼠随机分为实验组、对照组和单纯材料组,均拔除右侧下颌中切牙,实验组在拔牙窝内即刻植入纳米晶胶原基骨/活性肽 P17-骨形态发生蛋白2材料,对照组在拔牙窝内植入纳米晶胶原基骨/骨形态发生蛋白2材料,单纯材料组在拔牙窝内植入纳米晶胶原基骨材料.结果与结论:①牙槽嵴的相对长度:术后2,4,8周,实验组与对照组均大于单纯材料组(P 〈0.05),实验组与对照组比较差异无显著性意义(P 〉0.05).②组织学观察结果:术后2,4,8周,实验组与对照组新生血管和新骨形成的速度和质量均优于单纯材料组.③新生骨面积占骨缺损面积的百分比:术后2,4,8周,各组随时间增长不断增加,实验组与对照组大于单纯材料组(P 〈0.05),对照组大于实验组.表明纳米晶胶原基骨/活性肽 P17-骨形态发生蛋白2具有良好的骨诱导能力,拔牙后即刻植入可促进拔牙创的愈合,延缓剩余牙槽嵴的吸收.
    • 聂玮; 何伟
    • 摘要: 背景:烧结后的纳米羟基磷灰石结晶度很高,在体内很难降解;纳米β-磷酸三钙的降解速度太快,不利于体内生物组织在材料上附着,不利于引导成骨。目的:观察纳米羟基磷灰石/纳米β-磷酸三钙双相陶瓷人工骨的成骨及降解性能。方法:将36只青紫蓝兔随机分为实验组、对照组及空白组,制作左侧挠骨缺损模型,实验组与对照组分别植入纳米羟基磷灰石/纳米β-磷酸三钙双相陶瓷人工骨、纳米羟基磷灰石人工骨,空白组不植入任何材料。术后4,8,12周观察成骨和材料降解情况。结果与结论:①术后12周时 X 射线:实验组可见材料基本降解,连续性骨痂通过骨缺损部位。对照组材料未见明显降解,骨缺损处有骨痂修复。空白组骨缺损未见修复。②术后12周时组织学观察:实验组材料孔隙内以骨细胞和成骨细胞为主,有少量软骨细胞,出现散乱的骨松质,材料完全降解。对照组材料孔隙内以骨细胞为主,有少量成骨细胞和软骨细胞,材料未见明显降解。空白组可见纤维结缔组织及胶原纤维。③术后12周时扫描电镜观察:实验组材料降解,骨缺损部位被新生骨松质取代。对照组材料未见降解,骨缺损部位大都被新生骨松质取代。空白组无明显骨重建。表明纳米羟基磷灰石/纳米β-磷酸三钙双相陶瓷人工骨具有良好成骨能力及降解性能。%BACKGROUND: Nano-hydroxyapatite has a high crystal inity after sintering and difficultly degrades in vivo. Due to the high degradation rate, nano-β-tricalcium phosphate is not conducive for the biological tissue to attach on the material in vivo, which is not conducive to osteogenic induction. OBJECTIVE: To observe the bone formation and degradation performances of nano-hydroxyapatite/ nano-β-tricalcium phosphate biphasic ceramic artificial bone. METHODS: Thirty-six Chinchil a rabbits were randomly divided into experimental group, control group and blank group, and the rabbits were used to establish the left radius bone defect model. The rabbits in the experimental group and the control group were implanted with nano-hydroxyapatiteano-β-tricalcium phosphate biphasic ceramic artificial bone and nano-hydroxyapatite artificial bone respectively, and the blank group was not implanted with any materials. The bone formation and degradation performances were observed at 4, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION: X-ray film at 12 weeks after operation showed that the materials in the experimental group were basical y degraded, the continuity cal us grew through the bone defect site; the materials in the control group were not degraded obviously, and there was cal us at the bone defect site; the bone defect in the blank group was not repaired. Histological observation at 12 weeks after operation showed that the material pores in the experimental group were fil ed with bone cells and osteoblasts, as wel as a smal amount of chondrocytes, the scattered cancel ous bone appeared and the materials were completely degraded; the material pores in the control group were fil ed with bone cells, as wel as a smal amount of osteoblasts and chondrocytes, and the materials were not degraded completely; fibrous connective tissue and col agen fibers could be seen in the blank group. Scanning electron microscope observation at 12 weeks after operation showed that in the experimental group, the materials were degraded, and the bone defect sites were replaced with new bone cancel ous bone; materials in the control group were not degraded, and most of the bone defect sites were replaced with new cancel ous bone; no obvious bone reconstruction could be seen in the blank group. The results indicate that nano-hydroxyapatiteano-β-tricalcium phosphate biphasic ceramic artificial bone has good degradation performances.
    • 靳宪辉; 高春光; 崔胜杰; 刘桂花; 杨建博; 林月秋
    • 摘要: BACKGROUND: Heterogeneous tendon is an ideal method to repair tendon defects, but the post-transplantation rejection limits the application of heterogeneous tendons. OBJECTIVE: To investigate the effect of transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodi mide hydrochloride cross-linking acel ular tendon of Banna inbred-lines miniature pig for repairing the rabbit Achil es tendon defects. METHODS: Lateral resection of bilateral Achil es tendons from 40 Japanese rabbits was performed, and then the tendons were divided into experimental group and control group. The experimental group was repaired with transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodi mide hydrochloride cross-linking acel ular treated tendon of Banna mini-pig inbred-lines, and the control group was repaired with autologous tendon graft. RESULTS AND CONCLUSION: Histological observation showed that the cells in the new tissue of two groups were mainly the mononuclear fibroblast and fibrocytes, and the cells were oval or circular fibroblasts at 1-4 weeks after operation; new col agen formation could be seen around the cells in the cel aggregation area, and the col agen represented disorderly; focal cord-like mature col agen was distributed in island shape and formed the so-cal ed “col agen island”; at 12 weeks after operation, the cells were increasingly elongated and became spindle-shaped and long strip fibroblasts. Laboratory testing showed that there were no significant differences in white blood cells, C-reactive protein levels, hydroxyproline content and tensile strength between two groups. The results show that 1-ethy1-3-(3-dimethylaminopropy1) carbodi mide hydrochloride cross-linking acel ular tendon from Banna mini-pig inbred-lines can repair rabbit Achil es tendon defects successful y with good histocompatibility, less transplant rejection and strong biomechanical performance.%  背景:异体肌腱移植是目前修复肌腱缺损的理想方法,但移植后的排斥反应使其使用受到限制。目的:观察碳化二亚胺交联改性脱细胞处理的版纳近交系微型猪肌腱移植修复兔跟腱缺损的效果。方法:横向切除40只日本大白兔双侧跟腱,随机分组:实验组以碳化二亚胺交联改性脱细胞版纳近交系微型猪肌腱移植修复,对照组以自体肌腱移植修复。结果与结论:①组织学观察:两组新生组织内细胞主要都是单核的成纤维细胞和纤维细胞,术后1-4周主要是呈椭圆形或圆形的成纤维细胞,细胞聚集区的细胞周围有新生胶原形成,这些胶原的走向较紊乱;局灶性条索状成熟胶原呈岛状分布,形成所谓“胶原岛”;术后12周时细胞越来越拉长,成为梭形和长条形的纤维细胞。②实验室检测:两组白细胞、C-反应蛋白水平、羟脯氨酸含量及抗拉强度差异均无显著性意义。说明碳化二亚胺交联改性的脱细胞版纳近交系微型猪肌腱能成功修复兔跟腱缺损,且具有组织相容性好、移植排斥反应轻、生物力学性能强的优点。
    • 胡春奎; 陆建华; 陈昊; 熊家祥
    • 摘要: 背景:有研究报道病毒载体运载 N-甲基-D 天冬氨酸受体1小干扰 RNA 可有效缓解大鼠炎性疼痛,但病毒载体存在安全隐患。目的:探讨水溶性脂质体运载 N-甲基-D 天冬氨酸受体1小干扰 RNA 在体内外沉默 N-甲基-D 天冬氨酸受体1的效应和治疗神经病理性痛的可行性。方法:将 PC12随机分为阴性转染组、对照转染组和水溶性脂质体转染组,分别以 N-甲基-D-天冬氨酸受体1小干扰 RNA、聚乙烯亚胺与 N-甲基-D-天冬氨酸受体1小干扰 RNA 的复合物及水溶性脂质体与 N-甲基-D-天冬氨酸受体1小干扰 RNA 的复合物转染 PC12细胞,检测各组 N-甲基-D-天冬氨酸受体1基因 mRNA 及蛋白水平表达的变化。将48只 SD 大鼠随机分为假手术组、模型组、聚乙烯亚胺组及水溶性脂质体组,后3组建立大鼠神经病理性疼痛模型,并分别鞘内注射生理盐水、聚乙烯亚胺与N-甲基-D-天冬氨酸受体1小干扰 RNA 的复合物和水溶性脂质体与 N-甲基-D-天冬氨酸受体1小干扰 RNA 的复合物;假手术组只暴露坐骨神经。结果与结论:水溶性脂质体转染组 N-甲基-D-天冬氨酸受体1的 mRNA 与蛋白表达水平明显低于其他两组(P 0.05)。表明在体内条件下水溶性脂质体可有效运载 N-甲基-D-天冬氨酸受体1小干扰 RNA,抑制 N-甲基-D-天冬氨酸受体1的过度表达,还可减轻大鼠神经病理性痛。%BACKGROUND: Some studies have shown that viral vectors can carry N-methyl-D-aspartic acid receptors 1 (NR1) smal interference RNA (siRNA) to relieve inflammatory pain in rats, but the viral vectors are unsafe. OBJECTIVE: To examine the potential application of a non-viral gene carrier, water soluble lipopolymer (WSLP) for delivering siRNA targeting NR1 in vitro and in vivo and to determine whether WSLP-NR1siRNA complexes can be a new method for neuropathic pain treatment. METHODS: PC12 cells were randomly divided into three groups: group WSLP-negative NR1 siRNA (negative group), group polyethylenimine-NR1 siRNA (control transfection group) and group WSLP- NR1 siRNA (group WS). NR1 expressions were detected using reverse transcription-PCR and western blot analysis. Forty-eight healthy male Sprague-Dawley rats were randomly divided into four groups (n=12 in each group): sham operation group (sham group), neuropathic pain group (model group), group polyethylenimine-NR1 siRNA (group PEI) and group WSLP- NR1 siRNA (group WSLP). Neuropathic pain models were established in the latter three groups. Normal saline, PEI-NR1 siRNA complex and WSLP-NR1 siRNA were injected intrathecal y in model, PEI and WSLP groups, respectively, at 1 day after operation. Only the sciatic nerve was exposed in the sham group. RESULTS AND CONCLUSION: NR1 mRNA and protein expression significantly decreased in group WS as compared with negative and control transfection groups (P 0.05). These results demonstrate that WSLP not only efficiently delivers NR1 siRNA targeting NR1 in vivo and inhibits the expression of NR1, but also reduces neuropathic pain in rats.
    • 涂来勇; 阚瑞; 杨明坤; 徐滔; 盛伟斌; 马艳; 毕晓娟
    • 摘要: 背景:异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸具有良好的生物活性,在特定条件下可自组装成含IKVAV 纳米纤维凝胶支架,是一种天然的脊髓基质仿生材料.目的:进一步观察体外自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对骨髓源性神经干细胞增殖及向神经元分化的影响.方法:取第2代新生 SD 大鼠骨髓源性神经干细胞与自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶共培养作为实验组,设置神经干细胞单独培养为对照组、单独自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶为空白对照组,CCK-8法及流式细胞仪检测培养1,3,5,7,10,14 d 的细胞增殖与神经干细胞向神经元分化的比例;MTT 法检测自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对第2代新生 SD 大鼠骨髓源性神经干细胞的毒性.结果与结论:实验组不同时间点细胞增殖率及向神经元分化的细胞比例均高于对照组(P 〈0.05),两组细胞增殖均于第7天达峰值.MTT 法检测结果显示自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶无细胞毒性.表明异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶有较好的生物活性,可促进神经干细胞的增殖,提高神经干细胞向神经元分化的比例.
    • 涂来勇; 阚瑞; 杨明坤; 徐滔; 盛伟斌; 马艳; 毕晓娟
    • 摘要: BACKGROUND: Isoleucine-lysine-valine-alanine-valine has good biological activity, and under certain conditions, it can self-assemble into isoleucine-lysine-valine-alanine-valine nanofiber hydrogel scaffold which is a natural biomimetic material for the spinal cord matrix. OBJECTIVE: To observe the effects of self-assembled isoleucine-lysine-valine-alanine-valine nanofiber gel on proliferation and differentiation of bone marrow derived neural stem cells in vitro. METHODS: Passage 2 bone marrow derived neural stem cells were co-cultured with isoleucine-lysine-valine-alanine-valine as experimental group, neural stem cells cultured alone counted as control group, and isoleucine-lysine-valine-alanine-valine was set as blank control group. Cel proliferation rate and differentiation proportion of neural stem cells into neurons were measured at 1, 3, 5, 7, 10, 14 days after culture using Cel Counting Kit-8 and flow cytometry, respectively. 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay was used for detecting cytotoxicity of passage 2 bone marrow derived neural stem cells from new born Sprague-Dawley rats. RESULTS AND CONCLUSION: Cel proliferation rate and differentiation proportion of neural stem cells into neurons were significantly higher in the experimental group than in the control groups at each time (P < 0.05). Cel proliferation in the experimental and control groups peaked at day 7. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay detected no cytotoxicity of the isoleucine-lysine-valine-alanine-valine. Taken together, isoleucine-lysine-valine-alanine-valine nanofiber gel has a better biological activity, and can increase the differentiation proportion of neural stem cells into neurons.%  背景:异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸具有良好的生物活性,在特定条件下可自组装成含IKVAV 纳米纤维凝胶支架,是一种天然的脊髓基质仿生材料。目的:进一步观察体外自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对骨髓源性神经干细胞增殖及向神经元分化的影响。方法:取第2代新生 SD 大鼠骨髓源性神经干细胞与自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶共培养作为实验组,设置神经干细胞单独培养为对照组、单独自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶为空白对照组,CCK-8法及流式细胞仪检测培养1,3,5,7,10,14 d 的细胞增殖与神经干细胞向神经元分化的比例;MTT 法检测自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶对第2代新生 SD 大鼠骨髓源性神经干细胞的毒性。结果与结论:实验组不同时间点细胞增殖率及向神经元分化的细胞比例均高于对照组(P <0.05),两组细胞增殖均于第7天达峰值。MTT 法检测结果显示自组装异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶无细胞毒性。表明异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸纳米纤维凝胶有较好的生物活性,可促进神经干细胞的增殖,提高神经干细胞向神经元分化的比例。
    • 刘鉴峰; 褚丽萍; 王德芝; 贺欣; 徐宏艳; 刘金剑
    • 摘要: 背景:聚酰胺-胺型树枝状高分子纳米材料已被广泛应用于药物载体的研究,但由于整代聚酰胺-胺表面有大量带正电荷的氨基,具有一定的细胞毒性。目的:观察乙酰化对聚酰胺-胺细胞毒性的影响。方法:①细胞增殖检测:采用 MTT 法检测在含0,0.125,0.25,0.5,1,2,4μmol/L 乙酰化聚酰胺-胺的培养液中人胚肾293T 细胞的增殖。②细胞形态:倒置荧光显微镜观察在含4μmol/L 乙酰化聚酰胺-胺的培养液中人胚肾293T 细胞的形态。③细胞周期:流式细胞术检测在含0,5,10,15,20 mg/L 乙酰化聚酰胺-胺的培养液中人胚肾293T 细胞的细胞周期。结果与结论:聚酰胺-胺对293T 细胞具有一定的细胞毒性,在4μmol/L 浓度下48 h 的细胞存活率仅为52%,而乙酰化可显著降低聚酰胺-胺的细胞毒性(P <0.01);聚酰胺-胺孵育的细胞发生团缩,伸展性变差,而乙酰化聚酰胺-胺孵育的293T 细胞与正常培养细胞基本一致,具有良好的伸展性;乙酰化聚酰胺-胺对细胞周期无影响,而聚酰胺-胺在20 mg/L 较高质量浓度时可使细胞 S 期产生阻滞。表明乙酰化可以降低聚酰胺-胺的细胞毒性。%BACKGROUND: Polyamidoamine dendrimer nanomaterials have been widely used in drug carrier research, but there are many electropositive amino groups on the surface of the entire generation polyamidoamine, resulting in certain cytotoxicity.OBJECTIVE: To study the influence of acetylation on polyamidoamine cytotoxicity. METHODS: (1) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay: the cel proliferation of 293T cells incubated with acetylated polyamidoamine under 0, 0.125, 0.25, 0.5, 1, 2, 4 μmol/L concentrations was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. (2) Cel morphology: the cel morphology of 293T cells incubated with 4 μmol/L acetylated polyamidoamine was observed by inverted fluorescence microscope. (3) Cel cycle: the cel cycle of 293T cells incubated with acetylatedpolyamidoamine under 0, 5, 10, 15, 20 mg/L concentrations was detected by flow cytometry. RESULTS AND CONCLUSION: Polyamidoamine had cytotoxicity to 293T cells. The cel viability at 4 μmol/L concentration after 48 hours incubation was only 52%, and the acetylation could significantly decrease the cytotoxicity of polyamidoamine (P < 0.01). 293T cells incubated with polyamidoamine shrank and had bad stretching, while 293T cells incubated with acetylated polyamidoamine had good stretching. Acetylated polyamidoamine had no significant effect on the cel cycle, but polyamidoamine at 20 mg/L could block the cel cycle at S stage. Al the results show that acetylation can decrease the cytotoxicity of polyamidoamine.
    • 游玉华; 张朝平
    • 摘要: 背景:近年来,国内外学者对金属离子与氨基酸作用的研究很广泛,但有关银离子与氨基酸作用的研究相对较少.目的:应用 X 射线衍射、透射电镜、红外光谱对合成的组氨酸-银固体物种的表面结构进行表征.方法:在微乳液中制备出组氨酸-银配合物的固体物种,并用 X 射线衍射、透射电镜、红外光谱对其结构与形貌进行表征.结果与结论:红外光谱分析显示组氨酸中的咪唑环参与了同银离子的成键作用.X射线衍射图谱测得组氨酸-银晶粒的平均粒径为34.6 nm.透射电镜形貌图表明组氨酸-银配合物微粒形成莓球状集合体而被吸附于柱状物表面,配合物微粒的大小都在100 nm 以内.
    • 周新文; 刘希云; 赵晶
    • 摘要: 背景:临床上多采用平行或锥形玻璃纤维根管桩修复根管治疗后的残根残冠,但两种形态根管桩的临床效果还有待证实.目的:评价残冠修复时使用平行及锥形玻璃纤维根管桩的临床疗效.方法:选择残冠患者121例,共156颗患牙,经完善的根管治疗后采用掷硬币法随机分为两组,分别采用平行玻璃纤维根管桩与锥形玻璃纤维根管桩重建桩核,然后以全瓷或金属烤瓷冠修复.术后每半年复查1次,分别对修复体的稳固性、边缘密合性、牙周组织情况、咬合功能等方面进行临床评价,观察期为2年.结果与结论:121例患者均完成2年的随访观察.平行玻璃纤维根管桩组根管桩自牙颈部折断1例,治疗成功率为98.7%;锥形玻璃纤维根管桩组根管桩折断1例,根管桩连同全冠完整脱落1例,经重新粘接后行使功能良好,治疗成功率为97.5%;两组间治疗成功率差异无显著性意义(P 〉0.05).说明两种形态的玻璃纤维根管桩均可取得令人满意的临床效果,但远期疗效尚有待进一步观察.
  • 查看更多

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号