碳化二亚胺交联改性脱细胞异种猪肌腱修复跟腱缺损*

摘要

BACKGROUND: Heterogeneous tendon is an ideal method to repair tendon defects, but the post-transplantation rejection limits the application of heterogeneous tendons. OBJECTIVE: To investigate the effect of transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodi mide hydrochloride cross-linking acel ular tendon of Banna inbred-lines miniature pig for repairing the rabbit Achil es tendon defects. METHODS: Lateral resection of bilateral Achil es tendons from 40 Japanese rabbits was performed, and then the tendons were divided into experimental group and control group. The experimental group was repaired with transplantation of 1-ethy1-3-(3-dimethylaminopropy1) carbodi mide hydrochloride cross-linking acel ular treated tendon of Banna mini-pig inbred-lines, and the control group was repaired with autologous tendon graft. RESULTS AND CONCLUSION: Histological observation showed that the cells in the new tissue of two groups were mainly the mononuclear fibroblast and fibrocytes, and the cells were oval or circular fibroblasts at 1-4 weeks after operation; new col agen formation could be seen around the cells in the cel aggregation area, and the col agen represented disorderly; focal cord-like mature col agen was distributed in island shape and formed the so-cal ed “col agen island”; at 12 weeks after operation, the cells were increasingly elongated and became spindle-shaped and long strip fibroblasts. Laboratory testing showed that there were no significant differences in white blood cells, C-reactive protein levels, hydroxyproline content and tensile strength between two groups. The results show that 1-ethy1-3-(3-dimethylaminopropy1) carbodi mide hydrochloride cross-linking acel ular tendon from Banna mini-pig inbred-lines can repair rabbit Achil es tendon defects successful y with good histocompatibility, less transplant rejection and strong biomechanical performance.%　　背景：异体肌腱移植是目前修复肌腱缺损的理想方法，但移植后的排斥反应使其使用受到限制。目的：观察碳化二亚胺交联改性脱细胞处理的版纳近交系微型猪肌腱移植修复兔跟腱缺损的效果。方法：横向切除40只日本大白兔双侧跟腱，随机分组：实验组以碳化二亚胺交联改性脱细胞版纳近交系微型猪肌腱移植修复，对照组以自体肌腱移植修复。结果与结论：①组织学观察：两组新生组织内细胞主要都是单核的成纤维细胞和纤维细胞，术后1-4周主要是呈椭圆形或圆形的成纤维细胞，细胞聚集区的细胞周围有新生胶原形成，这些胶原的走向较紊乱；局灶性条索状成熟胶原呈岛状分布，形成所谓“胶原岛”；术后12周时细胞越来越拉长，成为梭形和长条形的纤维细胞。②实验室检测：两组白细胞、C-反应蛋白水平、羟脯氨酸含量及抗拉强度差异均无显著性意义。说明碳化二亚胺交联改性的脱细胞版纳近交系微型猪肌腱能成功修复兔跟腱缺损，且具有组织相容性好、移植排斥反应轻、生物力学性能强的优点。