组织构建细胞学实验
组织构建细胞学实验的相关文献在2012年到2015年内共计72篇,主要集中在基础医学
等领域,其中期刊论文72篇、专利文献332522篇;相关期刊1种,包括中国组织工程研究等;
组织构建细胞学实验的相关文献由189位作者贡献,包括刘瑞瑾、吴新荣、丁俐文等。
组织构建细胞学实验—发文量
专利文献>
论文:332522篇
占比:99.98%
总计:332594篇
组织构建细胞学实验
-研究学者
- 刘瑞瑾
- 吴新荣
- 丁俐文
- 丁洁
- 万东方
- 乔卫平
- 任明明
- 何援利
- 余淦
- 余虓
- 傅君舟
- 党木仁
- 冯勇
- 冯钢
- 刘佳
- 刘先宁
- 刘勇
- 刘妍
- 刘文杰
- 刘斌
- 刘木彪
- 刘湘泽
- 叶章群
- 叶静
- 吕仁发
- 吕国枫
- 吕夷松
- 吕德成
- 吴洁
- 吴补领
- 周义军
- 周勇
- 周姗姗
- 周聪
- 周萍
- 和静
- 姚军平
- 姚友生
- 姚小梅
- 姚文龙
- 孔令平
- 孙旗
- 孙闵
- 宋兴华
- 宋国立
- 尹琳
- 崔淯夏
- 庞奕辉
- 康佳丽
- 张传汉
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贾英民;
李瑞玉;
武密山;
霍瑞楼;
李彬
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摘要:
背景:补肾方药的动物及细胞实验研究提示具有防治骨质疏松及改善骨代谢作用,但补肾方药研究具有相当的复杂性,目前复方中药研究多采用的血清药物代谢动力学的方法却具有局限性,使有效作用成分及药物物质基础尚不确定。目的:通过采用补肾方药有效成分配伍,在不同浓度和时间下,对新生 SD 大鼠成骨细胞进行干预性培养,并对其增殖与分化情况进行测定,从而确定补肾方药对成骨细胞时效和量效关系,为补肾方药防治骨质疏松症提供实验依据。方法:体外培养原代新生24 h的SD大鼠成骨细胞,采用补肾方药的“补药”和“泻药”有效化学成分不同比例配伍,实验分3组,即补>泻组、补泻组、补泻组具有明显促增殖作用(P泻组、补泻组具有明显促增殖作用最强(P泻组具有促碱性磷酸酶分泌作用,质量浓度为20μg/L,在培养24 h以补泻组促明显的促碱性磷酸酶分泌作用最佳(P泻组配伍比例对成骨细胞增殖、分化作用最强,且存在时-效及量-效关系。%BACKGROUND:Animal and cel studies have shown that the Kidney Recipe can prevent and treat osteoporosis and improve bone metabolism, but this recipe is complicated. Recent studies on compound Chinese medicine mainly focused on serum drug metabolism and pharmacokinetics, which has limitations, and the effective ingredient and pharmaceutical material basis are uncertain. OBJECTIVE:In the different concentrations and time, by using different compatibility proportion of active ingredients of Kidney Recipe, osteoblasts from neonatal Sprague-Dawley rats were subjected to culture intervention. The proliferation and differentiation of osteoblasts were determined so as to identify time-effect and dose-effect relationship of Kidney Recipe on osteoblasts and to provide experimental evidences for prevention and treatment of osteoporosis. METHODS:Primary neonatal 24-hour osteoblasts of Sprague-Dawley rats were cultured in vitro. Herbs“tonic”and“cathartic”active chemical components of different proportion were used. The experiment contained three groups:Tonics Medicine (T)>Cathartic Medicine (C) group, TC group and TC group (PC and TC group (PC group. At mass concentration of 20μg/L and 24 hours of culture, the promoting effect on alkaline phosphatase secretion was most significant in the TC group (PC group in the time-effect and the dose-effect relationship.
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杨振村;
王增亮;
党木仁;
林令超;
赛力克·对山拜
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摘要:
背景:有研究表明脑血管畸形中存在管壁结构基质蛋白的表达差异,但血管畸形间结构基质蛋白的差异及结构蛋白差异与畸形血管管壁异形关系少有报道.目的:观察基质结构蛋白在脑血管畸形中的差异性表达.方法:对50例脑血管畸形手术标本和34例正常颞浅动脉标本进行苏木精-伊红染色,并应用美国Santa Cruz公司生产单/多克隆抗体对4种基质结构蛋白-Ⅳ型胶原蛋白、a-血管平滑肌蛋白、层粘连蛋白和纤维连接蛋白免疫组织化学染色.结果与结论:苏木精-伊红染色显示,脑血管畸形壁结构与正常血管相比呈现明显紊乱和构成异形性;且脑血管畸形中,脑动静脉畸形与海绵状血管瘤结构存在明显差异.免疫组织化学染色显示:正常颞浅动脉和畸形血管中Ⅳ型胶原和a-血管平滑肌蛋白全部呈阳性表达且无显著性差异,但结构排列差异明显;正常颞浅动脉与畸形血管层粘连蛋白和纤维连接蛋白表达阳性率差异有显著性意义(P<0.05),正常血管表达更多层粘连蛋白,畸形血管表达更多纤维连接蛋白;畸形血管中脑动静脉畸形和海绵状血管瘤间层粘连蛋白和纤维连接蛋白的阳性率表达差异有显著性意义(P<0.05),脑动静脉畸形血管表达更多层粘连蛋白,海绵状血管瘤血管表达更多纤维连接蛋白.结果证实:与正常血管相比,脑血管畸形者管壁结构存在明显异形性,结构蛋白Ⅳ型胶原和a-血管平滑肌蛋白结构明显紊乱,且低表达层粘连蛋白而高表达纤维连接蛋白,这种差异可能是导致脑血管壁结构异常的原因.
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杨振村;
王增亮;
党木仁;
林令超;
赛力克·对山拜
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摘要:
背景:有研究表明脑血管畸形中存在管壁结构基质蛋白的表达差异,但血管畸形间结构基质蛋白的差异及结构蛋白差异与畸形血管管壁异形关系少有报道.目的:观察基质结构蛋白在脑血管畸形中的差异性表达.方法:对50例脑血管畸形手术标本和34例正常颞浅动脉标本进行苏木精-伊红染色,并应用美国Santa Cruz公司生产单/多克隆抗体对4种基质结构蛋白-Ⅳ型胶原蛋白、a-血管平滑肌蛋白、层粘连蛋白和纤维连接蛋白免疫组织化学染色.结果与结论:苏木精-伊红染色显示,脑血管畸形壁结构与正常血管相比呈现明显紊乱和构成异形性;且脑血管畸形中,脑动静脉畸形与海绵状血管瘤结构存在明显差异.免疫组织化学染色显示:正常颞浅动脉和畸形血管中Ⅳ型胶原和a-血管平滑肌蛋白全部呈阳性表达且无显著性差异,但结构排列差异明显;正常颞浅动脉与畸形血管层粘连蛋白和纤维连接蛋白表达阳性率差异有显著性意义(P<0.05),正常血管表达更多层粘连蛋白,畸形血管表达更多纤维连接蛋白;畸形血管中脑动静脉畸形和海绵状血管瘤间层粘连蛋白和纤维连接蛋白的阳性率表达差异有显著性意义(P<0.05),脑动静脉畸形血管表达更多层粘连蛋白,海绵状血管瘤血管表达更多纤维连接蛋白.结果证实:与正常血管相比,脑血管畸形者管壁结构存在明显异形性,结构蛋白Ⅳ型胶原和a-血管平滑肌蛋白结构明显紊乱,且低表达层粘连蛋白而高表达纤维连接蛋白,这种差异可能是导致脑血管壁结构异常的原因.
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李敏;
刘佳;
钟玉华;
彭福华
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摘要:
背景:墨西哥钝口螈脊髓切断可以再生,再生过程伴随胶质细胞数目及分布的改变,研究墨西哥钝口螈脊髓全切后胶质细胞的变化,对进一步探讨其脊髓切断再生机制有重要意义。目的:观察墨西哥钝口螈脊髓全切后小胶质细胞、星形胶质细胞及少突胶质细胞的变化。方法:选用成年墨西哥钝口螈,分为脊髓全切组和对照组,利用免疫组织化学法观察脊髓全切后1,3和10 d 的损伤脊髓及周围区 cd11b 标记的小胶质细胞、胶质细胞原纤维酸性蛋白标记的星形胶质细胞及髓鞘碱性蛋白标记的少突胶质细胞的变化。结果与结论:脊髓全切后短期内 cd11b 染色阴性;脊髓损伤后胶质细胞原纤维酸性蛋白及髓鞘碱性蛋白阳性细胞染色强度,1 d 组阳性细胞染色强度与对照组比较无显著差异,3及10 d 组阳性细胞染色强度较对照组低。墨西哥钝口螈小胶质细胞染色阴性,可能存在不同于哺乳动物的标记蛋白;脊髓全切后3及10 d 在损伤脊髓及周围区的胶质细胞原纤维酸性蛋白及髓鞘碱性蛋白阳性细胞染色强度较对照组低,提示钝口螈脊髓急性损伤早期未见星形胶质细胞及少突胶质细胞增生,无胶质瘢痕形成。%BACKGROUND: The spinal cord of Ambystoma mexicanum can regenerate after transection, and the number and distribution of gliacytes alter during regeneration. So it is important to observe the changes of gliacytes fol owing spinal cord transection in Ambystoma mexicanum in order to further discuss the mechanism of spinal cord regeneration. OBJECTIVE: To observe the changes of microglias, astrocytes and oligodendrocytes fol owing spinal cord transaction in Ambystoma mexicanum. METHODS: Adult Ambystoma mexicanum was selected and divided into spinal cord transection group and control group. Immunohistochemistry assay was performed to observe the changes in cd11b-labeled microglias, glial fibril ary acidic protein labeled astrocytes and myelin basic protein labeled oligodendrocytes in the injured region and peripheral region at 1, 3 and 10 days after spinal cord transection. RESULTS AND CONCLUSION: After transaction, the staining results of cd11b were negative. The staining intensity results of glial fibril ary acidic protein and myelin basic protein positive cells at 3 and 10 day were lower to the control group, but showed no significant difference from the control group at 1 day after transection. Microglias were negative in Ambystoma mexicanum, indicating the marker proteins of microglia in salamander may be different from those in the mammals. The staining intensity results of glial fibril ary acidic protein and myelin basic protein positive cells at 3 and 10 days were lower to the control group, which suggested that astrocytes and oligodendrocytes hyperplasia were not seen and no glial scar formed in early stage of spinal cord injury in Ambystoma mexicanum.
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汪龙;
吕国枫
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摘要:
BACKGROUND: Studies on antioxidant genes Thioredoxin (TRX) have attracted more attention gradual y, but relevant studies from the aspect of gene therapy are fewer. OBJECTIVE: To study the protective effect of cells expressing the corresponding proteins in TRX transfected Neuro-2A cells and to analyze the possible mechanism underlying the protective effect. METHODS: Plasmids PIRES2-EGFP-TRX were used to transfect Neuro-2A cells and to establish a cel line which could stably express TRX proteins identified by reverse transcription-PCR. Different concentrations of hydrogen peroxide were used to treat normal cells and transfected cells for the establishment of oxidative stress models. Cel morphology, cellsurvival, glutathione concentration and DNA strand breaks situation were observed after oxidative damage. RESULTS AND CONCLUSION: Normal cells and transfected cells were both damaged by hydrogen peroxide; however, the transfected cells were superior to the normal cells in cel damage, cellsurvival, intracel ular glutathione level and degree of DNA strand breaks. These findings indicate that TRX gene in Neuro-2A cells can be reconstructed and expressed successful y, which plays a certain protective. This effect may be achieved through scavenging oxygen free radicals, maintaining intracel ular glutathione levels, thereby protecting cel ular DNA against oxidative damage.% 背景:目前对抗氧化基因硫氧还蛋白的研究逐步受到重视,但从基因治疗的角度对其研究较少。目的:采用硫氧还蛋白转染 Neuro-2A 细胞,观察细胞内表达相应蛋白因子对细胞的具体保护效果,并分析其发挥保护作用的可能机制。方法:以质粒 PIRES2-EGFP-TRX 转染 Neuro-2A 细胞,经 RT-PCR 鉴定,建立能够稳定表达硫氧还蛋白的细胞株。利用不同浓度的 H2O2处理正常细胞及转染细胞,建立氧化应激模型。观察受到氧化损伤后两组细胞的形态学、存活率、还原型谷胱甘肽的浓度及细胞内 DNA 链断裂情况。结果与结论:经 H2O2作用后,两组细胞形态均出现损伤,但转染组细胞比正常组细胞损伤减轻、细胞存活率升高、细胞内还原型谷胱甘肽水平增高、DNA 链断裂程度减轻。说明人类硫氧还蛋白基因可以在 Neuro-2A 细胞中被重组并顺利表达,对细胞具有一定的保护作用;这一作用可能是通过清除氧自由基,维持细胞内还原型谷胱甘肽水平,从而保护细胞 DNA 免受氧化性损伤来实现的。
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方秋红;
税朝祥;
王尧尧;
王瑞芹;
王晶;
马迎民
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摘要:
BACKGROUND: In addition to the supporting function, lung fibroblasts play a pivotal role in the process of lung tissue injury and repair through proliferation, contraction, chemotaxis and secretion of the extracel ular matrix. OBJECTIVE: To investigate the effects of trypsin and dexamethasone on the proliferation and cytoskeleton protein expression of lung fibroblasts. METHODS: Human lung fibroblasts were isolated and cultured in vitro fol owed by trypsin (0, 0.5, 1.0, 5, 10 mg/L) treatment for 24 hours or dexamethasone (10-9-10-6 mol/L) treatment for 72 hours. The expression of vimentin and α-smooth muscle actin was detected by immunoblotting. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was utilized for measuring fibroblasts proliferation. RESULTS AND CONCLUSION: Trypsin at lower concentrations (0.1-0.5 mg/L) significantly stimulated lung fibroblasts proliferation, while higher concentrations (1-10 mg/L) of trypsin inhibited lung fibroblasts proliferation. No effect on fibroblasts proliferation was observed with dexamethasone. Trypsin significantly increased the expression ofα-smooth muscle actin in a dose-dependent manner, but had no influence on vimentin expression. Dexamethasone (10-9-10-6 mol/L) significantly inhibited vimentin expression in a concentration-dependent manner. The data reveal that trypsin and glucocorticosteroids may participate in the tissue remodeling process of chronic inflammatory airway diseases through affecting lung fibroblasts proliferation and the expression of cytoskeleton protein.% 背景:肺成纤维细胞不但是肺组织的结构支持细胞,还能够通过增殖、收缩、趋化及分泌细胞外基质等多种功能在肺组织的炎症损伤-修复过程中发挥关键作用。目的:观察原代培养的人肺成纤维细胞在胰蛋白酶或糖皮质激素作用下增殖特性与骨架蛋白表达的变化。方法:采用人肺成纤维细胞体外培养,胰酶处理24 h,终浓度分别为0,0.5,1.0,5,10 mg/L 胰酶;地塞米松处理72 h,在有血清培养条件下进行,浓度范围10-9-10-6 mol/L。MTT 法测定成纤维细胞增殖情况;免疫印迹方法测定细胞波形蛋白和肌动蛋白的表达。结果与结论:胰蛋白酶在低浓度(0.1-0.5 mg/L)时刺激肺成纤维细胞增殖;而较高浓度(1-10 mg/L)明显抑制细胞生长。地塞米松对肺成纤维细胞增殖作用的影响不明显。胰蛋白酶显著上调肺成纤维细胞α平滑肌肌动蛋白的表达,但对波形蛋白的表达无明显影响;地塞米松(10-9-10-6 mol/L)显著抑制波形蛋白的表达。结果表明在慢性阻塞性肺病等支气管肺慢炎症性疾病的发病过程中,胰蛋白酶和糖皮质激素可以通过参与成纤维细胞增殖和骨架蛋白的表达发挥作用。
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- 西安交通大学医学院第一附属医院
- 公开公告日期:2022-01-04
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摘要:
一种全自动细胞学涂片固定仪,包括传送装置、提片装置、制片装置、涂片固定装置和回收装置;提片装置和制片装置相配合完成细胞样本涂片的制作,涂片固定装置完成细胞样本涂片的湿固定。一种全自动细胞学涂片的制备方法,由设备在介入超声穿刺现场完成即时细胞液涂片及快速湿固定的工作,细胞涂片完成效率更高,涂片质量更加稳定。全自动细胞学涂片固定仪完全替代了传统需要人工操作的步骤,实现了涂片与固定的一键式操作,从而在不增加人员的基础上,由超声医生自主监管,高效完成更多的甲状腺FNA细胞学采集工作,协助获得高品质的细胞学病理诊断结果。
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