T淋巴细胞,调节

摘要： 目的 探讨血必净注射液(简称血必净)与其组分芍药苷对脓毒症大鼠脾脏调节性T细胞(Treg)免疫功能及大鼠生存率的影响. 方法 (1)免疫磁珠法分离提纯3只9～12周龄SD雄性大鼠(鼠龄、品系、性别下同)脾脏CD4+ CD25+ Treg和CD4+T细胞.按照随机数字表法(分组方法下同)将CD4+ CD25+ Treg分为空白对照组、单纯CD3/CD28组、单纯内毒素/脂多糖(LPS)组、LPS+血必净组、LPS+芍药苷组,每组6孔.单纯CD3/CD28组、单纯LPS组、LPS+血必净组及LPS+芍药苷组细胞采用加入1.25 μg CD3和2.5 μg CD28的含体积分数10％胎牛血清的RPMI 1640培养基培养24 h后,单纯LPS组、LPS+血必净组及LPS+芍药苷组细胞加入1μg/mL LPS 1 μL,并立即在LPS+血必净组细胞中加入5 mg/mL血必净1μL、在LPS+芍药苷组细胞中加入80 μmol/L芍药苷1μL,均继续培养72 h.空白对照组细胞加入等体积含体积分数10％胎牛血清的RPMI 1640培养基培养96 h.采用流式细胞术检测CD4+ CD25+ Treg的细胞毒性T淋巴细胞相关抗原4(CTLA4)及叉头翼状螺旋转录因子3(Foxp3)表达、CD4+ CD25+ Treg凋亡率,酶联免疫吸附测定(ELISA)法检测CD4+ CD25+ Treg培养上清液中白细胞介素10(IL-10)水平.将CD4+T细胞分为空白对照'组、单纯CD3/CD28'组、单纯LPS'组、LPS+血必净'组、LPS+芍药苷'组,每组6孔,加入前述相应组处理后CD4+ CD25+ Treg共培养72 h后,流式细胞术检测CD4+T细胞增殖活性,ELISA法检测CD4+T细胞培养上清液中IL-4水平.(2)将120只大鼠分成假手术组、单纯脓毒症组、脓毒症+血必净组和脓毒症+芍药苷组,每组30只.单纯脓毒症组、脓毒症+血必净组和脓毒症+芍药苷组大鼠采取盲肠结扎穿孔术构建脓毒症模型,假手术组大鼠单纯开腹模拟手术.脓毒症+血必净组大鼠术后经尾静脉注射血必净4 mL/kg,2次/d;脓毒症+芍药苷组大鼠术后经尾静脉注射芍药苷978 μg,2次/d.分别观察并记录术后1、2、3、4、5、6、7d的4组大鼠生存率,并绘制Kaplan-Meier生存曲线.对数据行单因素方差分析、LSD-t检验,对生存曲线行Log-rank(Mantel-Cox)检验. 结果 (1)与空白对照组比较,单纯LPS组CD4+ CD25+ Treg的CTLA4、Foxp3表达水平明显升高(t=27.19、17.00,P＜0.01),细胞培养上清液中IL-10水平明显升高(t=40.76,P＜0.01).与单纯LPS组比较,LPS+血必净组、LPS+芍药苷组CD4+ CD25+ Treg的CTLA4、Foxp3表达水平明显降低(LPS+血必净组=31.03、11.27,tLPS+芍药苷组=5.79、5.64,P＜0.01),细胞培养上清液中IL-10水平明显降低(t=15.49、4.20,P ＜0.01).与空白对照组比较,单纯LPS组CD4+ CD25+ Treg凋亡率明显下降(t=6.02,P＜0.01);与单纯LPS组比较,LPS+血必净组、LPS+芍药苷组CD4+ CD25+ Treg凋亡率明显增加(t=20.32、8.60,P＜0.01).(2)与单纯CD3/CD28'组比较,单纯LPS'组CD4+T细胞增殖率明显降低(t=22.47,P＜0.01),细胞培养上清液中IL-4水平明显升高(t=3.51,P＜0.01).与单纯LPS'组比较,LPS+血必净'组、LPS+芍药苷'组CD4+T细胞增殖率均明显增加(t=16.31、11.48,P＜0.01),细胞培养上清液中IL4水平无明显变化.(3)假手术组、单纯脓毒症组、脓毒症+血必净组、脓毒症+芍药苷组大鼠术后7d生存率分别为100％ (30/30)、30％(9/30)、57％(17/30)、47％(14/30).与单纯脓毒症组比较,脓毒症+血必净组大鼠术后7d内生存率明显升高(x2=4.34,P＜0.05),脓毒症+芍药苷组大鼠术后7d内生存率无明显变化. 结论 血必净及其组分芍药苷均能逆转脓毒症介导的大鼠Treg免疫抑制功能上调及凋亡抵抗,并改善效应性T细胞增殖能力.芍药苷对脓毒症大鼠生存率的改善效应明显弱于血必净.

摘要： 妊娠期糖尿病的发病率逐年上升.目前认为除了胰岛素抵抗,全身低度炎症在妊娠期糖尿病的发病机制中也起着重要作用.这种全身慢性炎症状态与辅助性T细胞(Th)1、Th2、Th17、调节性T细胞免疫调节偏移导致促炎因子相对性分泌增多有关.本文主要针对近年辅助性T细胞及其分泌的细胞因子在妊娠期糖尿病中的作用进行综述.

摘要： 子宫内膜异位症(EMs)是目前严重困扰育龄期女性的常见疾病,因其发病机制复杂,病因尚未阐明,以及其易种植、复发等类似肿瘤的特性,迄今尚无确切诊治手段.最新研究发现,EMs属于一种自身免疫性疾病,与T细胞免疫密切相关.CD4+T细胞的2个重要亚型,辅助性T细胞(Th)17与调节性T细胞(Treg)比例(Th17/Treg)失衡及其所致的腹腔内环境稳态失衡,均与EMs发生、病变程度及其导致不孕等临床特征有关.笔者拟就Th17/Treg与免疫内环境失衡在EMs中的最新研究现状进行阐述.

摘要： 目的 探讨外周血调节性T细胞(regulatory T cell,Tregs)线粒体自噬异常参与重症肌无力(myas-thenia gravis,MG)发病的细胞代谢机制.方法 选择2016年10月至2018年4月作者医院收治的MG患者15例,另选择同期体检的15名健康成年人作为对照组.采用Ficoll密度梯度离心法分离出人外周血单个核细胞(PBMC),选用基于分离柱的人CD4+CD25+Tregs分选试剂盒分离得到Tregs,以健康人群的Tregs作为对照组(health control,HCs),将MG患者的Tregs分为MG组 、MG经羰基氰化物间氯苯腙(CCCP)诱导组(MG-CCCP)、MG经环孢素(CsA)诱导组(MG-CsA).采用流式细胞仪检测各组Tregs线粒体质量 、活性氧(reactive oxygen species,ROS)、细胞凋亡水平,采用酶标仪检测各组Tregs乳酸水平.结果 与对照组比较,MG组线粒体受损比例 、细胞凋亡比例 、平均ROS水平 、乳酸水平升高(P<0.05),ROS正常细胞比例降低(P<0.05);与MG组比较,MG-CCCP组线粒体受损比例 、细胞凋亡比例 、乳酸水平降低(P<0.05),平均ROS水平 、ROS正常细胞比例升高(P<0.05);与MG组比较,MG-CsA组线粒体受损比例 、细胞凋亡比例 、乳酸水平 、平均ROS水平升高(P<0.05),而ROS正常细胞比例降低(P<0.05).结论 MG患者Tregs线粒体质量下降,导致其ROS释放增加,ROS正常细胞比例减少,糖酵解生成的乳酸增多,细胞凋亡增加,CCCP通过促进MG患者Tregs线粒体自噬,可清除部分受损线粒体,缓解代谢压力,减少细胞凋亡,而CsA可通过抑制MG患者Tregs线粒体自噬,加剧代谢紊乱,使细胞凋亡增加.

摘要： Myeloproliferative neoplasms (MPN) are a group of abnormal clonal diseases of hematopoietic stem cell,which characterized by sustained clonal proliferation of relatively mature differentiated one or more myeloid cells.MPN mainly include chronic myeloid leukemia (CML) and Ph-/MPN.In recent years,studies have reported that abnormal number and function of regulatory T cell (Treg) play an important role in the occurrence and development of MPN.Therefore,this article reviews recent research advances in the biological characteristics of Treg,relationship between number of Treg in CML patients and CML clinical phase,Sokal score,efficacy and transcription level of BCR-ABL fusion gene,as well as the role of Treg in the pathogenesis and progression of Ph-MPN.%骨髓增殖性肿瘤(MPN)是一组造血干细胞异常克隆性疾病,其特征为分化相对成熟的一系或多系骨髓细胞持续克隆性增殖.该病主要包括慢性髓细胞白血病(CML)和Ph MPN.近年文献报道,调节性T细胞(Treg)数量和功能的异常,在MPN的发生、发展中发挥重要作用.因此,笔者拟就Treg的生物学特征及其对MPN患者的作用,CML患者体内Treg数量与CML临床分期、Sokal评分、疗效及BCR-ABL融合基因转录水平的关系,以及Treg在Ph-MPN发病和进展中作用的研究进展进行综述.

摘要： 背景:Treg细胞是一种抑制性CD4+T细胞,人胎盘间充质干细胞具有T细胞免疫调节功能。目的:探讨人胎盘间充质干细胞上清液对CD4+T细胞和Treg细胞比例以及免疫调节功能的影响。方法:分离培养人胎盘间充质干细胞,建立其稳定传代细胞系。收集第4代人胎盘间充质干细胞及细胞上清培养液,流式细胞术检测细胞表面标志物CD90、CD73、CD45、HLA-DR的表达。采用人胎盘间充质干细胞上清液与细胞因子刺激剂联合培养正常人外周血单个核细胞6 h,流式细胞术检测CD4+T细胞和Treg细胞比例;ELISA方法检测细胞培养上清中转化生长因子β水平。结果与结论:①成功建立人胎盘间充质干细胞原代细胞系,且可稳定传代;②人胎盘间充质干细胞上清液能够显著降低CD4+T细胞表达比例(P<0.05),增强Treg细胞的表达比例(P<0.05),并上调其主要细胞因子转化生长因子β水平(P<0.01);③结果表明,人胎盘间充质干细胞上清液可以通过诱导CD4+T细胞向Treg细胞的转化而发挥免疫调节作用,有望成为一种免疫治疗的新选择。

摘要： Objective To investigate the change and significance of regulatory T cells (Tregs) in peripheral blood in patients with acute and chronic gout. Methods Flow cytometry was used to detect the ratio of Tregs in peripheral blood of healthy controls, patients with acute gout and patients with chronic gout. Enzyme linked immunosorbent assay (ELISA) was used to detect the levels of transforming growth factor (TGF)-βand interleukin (IL)-1βin plasma. Then, statistical analysis was conducted to analyze the changes and significance in different stages of gout, such as F test, Kruskal-Walls H test, q test and Pearson and Spearman correlation analysis. Results ① The percentage of CD4+CD25+Foxp+Treg/CD4+ T cells in peripheral blood was (1.22±0.27)％ in control group. While in patients with acute gout, it was (1.51±0.43)％, and (0.47±0.26)％in patients with chronic gout. There were statistical significant difference among the three groups ( F=101.39, P0.05). ③The con-centration of IL-1β in plasma in the control group was (4.8 ±1.3) ng/L, while that in patients with acute and chronic gout was (10.1±8.5) ng/L and (11.50±12.57) ng/L respectively, the difference between these three groupswas stati-sticallysignificant (P0.05) patients. ④ The percentage of Tregs in peripheral blood of gout patients was negatively correlated with the duration of disease and the number of gout attacks within six months (duration of disease: r =-0.381, P 0.05).③健康对照组血清IL-1β浓度为(4.8±1.3)ng/L、急性痛风组为(10.1±8.5)ng/L、慢性痛风组为(11.5±12.6)ng/L,3组间差异有统计学意义(χ2=7.54,P0.05).④痛风患者外周血调节性T细胞百分率与病程、6个月内发病次数均呈负相关(病程:r=-0.381,P<0.05;发病次数:r=-0.518,P<0.01),与血浆TGF-β、IL-1β浓度均无相关.结论 调节性T细胞在急性痛风中升高,参与了痛风炎症缓解;在慢性痛风中降低,可能与痛风迁延不愈有关.调节性T细胞在急、慢性痛风转换中起重要调控作用.

摘要： 目的 探讨多发性硬化(MS)患者外周血辅助性T细胞17(Th17)和Th17相关细胞因子IL-17和IL-23及调节性T细胞的水平.方法 急性发作复发缓解型MS患者(RRMS)组20例及对照组20名,分析Th17和调节性T细胞比率及IL-17和IL-23水平,比较2组Th17、调节性T细胞、IL-17和IL-23表达水平;给予甲泼尼龙治疗,比较治疗前后RRMS组Th17、调节性T细胞、IL-17和IL-23水平,并结合扩展残疾状况评分量表(EDSS)评分、头颅MRI增强病灶数目对RRMS组进行病情评估,采用IBM SPSS 17.0软件对数据进行统计分析.RRMS组与对照组计量资料比较采用独立样本t检验,计数资料比较采用χ2检验;RRMS组治疗前、治疗后的比较采用配对样本t检验;RRMS组治疗前变量采用Pearson相关分析.结果 RRMS组外周血中Th17细胞高于对照组[(2.10±0.45)％与(1.09±0.20)％;t=9.130,P0.05),Th17、IL-17、IL-23与头颅MRI增强数呈正相关(r=0.446,P0.05).结论 Th17/调节性T细胞表达失衡及Th17相关细胞因子IL-17和IL-23的高表达参与了MS的病理过程,可能作为治疗MS的新靶点.%Objective To investigate the levels of T helper cell 17 (Th17), Th17-related cytokines in-terleukin 17 (IL-17) and interleukin 23 (IL-23) and regulatory T cell (Treg) in relapsing remitting multiple sclerosis (RRMS). Methods In a case-control study, plasma was collected from RRMS patients (n=20) and healthy subjects as control group (n=20). The percentages of Th17 and Treg cells and the levels of IL-17 and IL-23 were tested. The levels of Th17, Treg, IL-17 and IL-23 of the two groups were compared. Patients were treated with methylprednisolone. The levels of Th17, Treg, IL-17 and IL-23 of multiple sclerosis (MS) patients b efore and after treatment were compared. Expanded disability status scale (EDSS) score and the number of Gd-enhancing lesions were evaluated in the case group. Statistical analysis was made by body mass index (IBM) statistical program for social sciences (SPSS) 17.0 software. Independent sample t test was conducted to compare the measurement data of the case group and the healthy control group, and enumeration data were compared by χ2 test; paired sample t test was performed to compare the data of the case group before and after treatment; Pearson correlation analysis was made forthe variables of the MS group before treatment. Results In the RRMS group, the percentage of Th17 cells in peripheral blood was significantly higher than the control group [(2.10±0.45)％vs (1.09±0.20)％](t=9.130, P0.05), and Th17, IL-17 and IL-23 were positively correlated with the number of Gd-enhancing lesions (Th17: r=0.446, P0.05). Conclusion Th17/Treg expression imbalance and Th17-related cytokines IL-17, IL-23 may participate in the pathological process of MS, and they might be therapeutic target for MS.

摘要： 目的 探讨DiR荧光染料标记的调节性T淋巴细胞(Tregs)在异种肝组织移植小鼠体内的归巢特征.方法 检测不同温育时间和不同质量浓度DiR染料标记的Tregs荧光强度,结合新型四唑化合物(MTS)实验检测细胞活力,从而确定最佳温育时间及染料质量浓度.采用流式细胞术验证DiR染料对Tregs功能及表型的影响.构建异种肝组织移植小鼠模型并免疫重建,回输Tregs后不同时间点进行小动物活体荧光成像.通过免疫组织化学分析免疫重建以及体内淋巴细胞分布情况.多组间比较采用单因素方差分析和Dunnett-t检验.结果 随着DiR浓度和温育时间增加,Tregs荧光强度增强,3 d达到高峰后逐渐减弱,5.00μg/ml及20.00μg/ml组相比对照组在各时间点的细胞活力显著下降(F=120.142～182.025,t=9.969～19.329,均P<0.05),温育30 min及60 min后,Tregs活力相较对照组也明显下降(F=21.826～301.968,t=6.897～40.016,均P<0.05),结合MTS实验结果得出最优标记条件为2.50μg/ml DiR染料温育5 min.免疫表型流式细胞术检测表明DiR染料不影响Tregs表型及功能.小动物荧光成像系统检测到Tregs可以汇聚于移植物区域.免疫组织化学分析结果表明Tregs能改善免疫重建后诱导的淋巴细胞浸润情况.结论 荧光成像可直接观察DiR染料标记的Tregs在小鼠体内的分布,是一种有前景的Tregs示踪的影像学手段.%Objective To explore the homing of DiR labeled regulatory T cells ( Tregs) in human-ized heterologous liver tissue transplantation mouse model. Methods The fluorescence intensities of Tregs labeled with different concentrations of DiR dye and different incubation times were measured, and the cell viability was measured by 3-( 4, 5-dimethylthiazol-2-yl )-5-( 3-carboxymethoxyphenyl )-2-( 4-sulfophenyl )-2H-tetrazolium ( MTS) assay to determine the optimal incubation time and dye concentration. The effect of DiR dye on the function and phenotype of Tregs was verified by flow cytometry. The xenogeneic liver tissue transplantation mouse model was constructed and the immune system was reconstituted. Small animal fluores-cence imaging was performed at different time points after infusion of Tregs. Immunohistochemistry analysis was used to analyze immune reconstitution and lymphocyte distribution in vivo. One-way analysis of variance and Dunnett-t test were used to analyze the data. Results With the increase of DiR concentration and incu-bation time, the fluorescence intensity of Tregs increased and gradually weakened after reaching the peak at 3 d. The cell viability of the 5. 00 μg/ml and 20. 00 μg/ml groups was significantly lower than that of the control group (culture medium) at various time points (F=120.142-182.025, t=9.969-19.329, all P<0. 05) . After incubation for 30 min and 60 min, the activity of Tregs was also significantly lower than that of the control group (F=21.826-301.968, t=6.897-40.016, all P<0.05). Tregs were finally co-incubated with DiR dye at a concentration of 2.50 μg/ml for 5 min, which was used further in vivo experiments. The flow cytometry showed that DiR dye did not affect the phenotype or the function of Tregs. The small animal fluorescence imaging showed that Tregs could locate in the graft area of mouse model. Immunohistochemical analysis showed that Tregs could improve lymphocyte infiltration induced by immune reconstitution. Conclu-sion After labeling Tregs with DiR dye, the distribution of Tregs can be directly observed by fluorescence imaging, which is a promising imaging method for Tregs tracer.

摘要： Immune thrombocytopenia (ITP) is an autoimmune hemorrhagic disease.The classic pathogenesis of ITP is that the production of autoantibodies against platelet membrane glycoproteins,which mediates platelet destruction.In recent years,it has been found that there are still many other mechanisms in ITP inducing thrombocytopenia and platelet damage,among which abnormal cellular immunity plays an important role.In cellular immunity,CD4+ CD25high CD127-regulatory T cells (Treg) are a group of T cell subsets that exist in human peripheral blood and spleen and can inhibit auto-reactive T cell responses;T helper cells (Th) 17 is a newly discovered T cell subset that secretes interleukin (IL)-17,involved in mediating inflammatory response,which is of great significance in autoimmune diseases.Several studies showed that Treg/Th17 imbalance and abnormal expression of specific transcription factors folkhead box protein (Foxp)3 and retinoic acid-related orphan nuclear receptor (ROR)-γt were observed in peripheral blood of ITP patients.DNA methylation induces Foxp3 gene silencing and abnormal activation of IL-6/STAT3 signaling pathway,which may be the mechanism leading to Treg/Th17 imbalance and causes of ITP.The proposed demethylation treatment may provide new ideas for the molecular targeted treatment of ITP,but its scientificity,effectiveness and safety still need to be confirmed by further studies.The author reviews literature on the role of Treg/Th17 imbalance and relevant gene methylation mechanism in the occurrence,development and treatment of ITP.%免疫性血小板减少性症(ITP)为一种自身免疫性出血性疾病,其经典发病机制为机体产生针对血小板膜糖蛋白的自身抗体,从而介导血小板的破坏.近年来,研究发现ITP亦存在其他多种机制共同介导血小板生成减少、破坏增多,其中细胞免疫异常发挥了重要作用.在细胞免疫中,CD4+ CD25high CD127-调节性T细胞(Treg)为一类存在于人外周血与脾中的T细胞亚群,具有抑制自身反应性T细胞应答的功能;而辅助性T细胞(Th)17为新近发现的、能够分泌白细胞介素(IL)-17的淋巴细胞亚群,参与介导炎症反应,在自身免疫性疾病中具有重要意义.多项研究结果显示,ITP患者外周血中Treg/Th17失衡,以及特异性转录因子叉头框蛋白(Foxp)3及维甲酸相关孤核受体(ROR)-γt表达异常.而DNA甲基化诱导Foxp3基因沉默、IL-6/STAT3信号通路异常活化,可能是导致Treg/Th17失衡及ITP发生的机制,由此提出的去甲基化治疗可能为ITP的靶向治疗提供新思路,但是其科学性、有效性及安全性仍需进一步研究证实.笔者拟就Treg/Th17失衡及相关基因甲基化机制在ITP发生、发展过程中所发挥的作用及相关治疗进行综述.

摘要： 一种活性化T淋巴细胞培养液及其活性化T淋巴细胞的制备方法，属于细胞培养技术领域。包括AT细胞的培养和AT细胞的收获两大步骤。上述一种活性化T淋巴细胞培养液及其活性化T淋巴细胞的制备方法，其通过使用Lymactin‑T抗CD3单克隆抗体配合IL‑2，IL‑6的方法，效率极高的激活T淋巴细胞并诱导期快速增殖；并使用PMA和离子霉素配合的方法激活T细胞；该方法具有效率高，纯度高等优点。

摘要： 提供一种淋巴细胞分离管及其制备方法、淋巴细胞分离方法，其中，所述的淋巴细胞分离管，包括管体、置于所述管体内的分离胶体和分离液，所述分离液在所述管体底部，所述分离胶体的密度小于所述分离液位于所述分离液上方；在25°C时，所述分离胶体的密度为1.0655‑1.0755g/cm

摘要： 本发明提供用于抗原特异性的免疫耐受或免疫抑制的药物组合物。本发明提供包含CD4阳性无反应性T细胞、及CD8阳性无反应性T细胞的药物组合物。在一些实施方式中，无反应性T细胞是通过能够抑制CD80和/或CD86与CD28的相互作用的抗体而诱导的。在特定的实施方式中，药物组合物可以还包含调节T细胞(例如，FOXP3阳性CD4阳性CD25阳性T细胞)。

摘要： 本发明公开了巨噬细胞迁移抑制因子(MIF)调节CTL活性的表达。在EL4肿瘤鼠模型中，来自于原发肿瘤鼠的培养的脾细胞在体外经抗原刺激后能够高水平分泌MIF。与单克隆抗体处理的对照细胞组相比，用抗MIF单克隆抗体中和处理后，平行脾细胞对肿瘤细胞的CTL反应显著增强，同时IFNγ表达水平上调。肿瘤组织学研究表明，用抗MIF抗体处理动物后，其CD4

摘要： 本发明涉及一种新型淋巴细胞群体、用于产生这些淋巴细胞的方法以及所述淋巴细胞在疾病治疗中的用途。

摘要： 本文提供的是从外周血单核细胞开始生产T细胞，例如细胞毒性T淋巴细胞的方法，该方法没有产生和分离抗原呈递细胞的独立步骤，并使用单轮次的抗原刺激。本文还提供的是使用所述细胞毒性T淋巴细胞，例如，来治疗癌症和/或病毒感染的方法。

摘要： 一种活性化T淋巴细胞培养液及其活性化T淋巴细胞的制备方法，属于细胞培养技术领域。包括AT细胞的培养和AT细胞的收获两大步骤。上述一种活性化T淋巴细胞培养液及其活性化T淋巴细胞的制备方法，其通过使用Lymacin‑T抗CD3单克隆抗体配合IL‑2，IL‑6的方法，效率极高的激活T淋巴细胞并诱导期快速增殖；并使用PMA和离子霉素配合的方法激活T细胞；该方法具有效率高，纯度高等优点。

摘要： 本申请公开了一种用于淋巴细胞定量检测标记物和定量检测方法。本申请标记物，为DNA片段或含有DNA片段的质粒，DNA片段5’到3’端依序包括V区、M区和J区，V区由前导区基因和/或V基因全部或部分序列组成，J区由J基因全部或部分序列组成，M区包括外源核苷酸片段。本申请的标记物，在免疫组库建库时添加到gDNA中一起建库测序，定量标记物获得淋巴细胞数目。本申请的淋巴细胞定量方法，简单快速、明白直接；对生物实验要求和依赖度不高；可分析得致病克隆在PBMC/BMMC/有核细胞的比例，使基于高通量测序监控致病克隆比例成为现实，对免疫相关疾病早期诊断、预后监控、指导临床治疗有重要意义。

摘要： 本发明提供了一种用于降低淋巴细胞归巢受体和/或淋巴细胞归巢配体的表达的组合物及应用该组合物的产品，涉及医药技术领域，大黄提取物和巴豆霜的组合物能够有效止泻，本发明通过实验证明，大黄提取物和巴豆霜的组合物能够降低淋巴细胞归巢受体和/或淋巴细胞归巢配体的表达，从而减少淋巴细胞归巢，减轻肠道炎症反应，进一步促进肠粘膜愈合，为大黄提取物和巴豆霜的组合物达到修复肠粘膜损伤提供又一证据，对溃疡性结肠炎的预防和/或治疗具有潜在价值。本发明提供的药物，包括大黄提取物和巴豆霜，可用于降低淋巴细胞归巢受体和/或淋巴细胞归巢配体的表达、减少淋巴细胞归巢、减轻肠道炎症反应和促进肠粘膜愈合，具有疗效好，副作用低的优点。

摘要： 提供一种淋巴细胞分离管及其制备方法、淋巴细胞分离方法，其中，所述的淋巴细胞分离管，包括管体、置于所述管体内的分离胶体和分离液，所述分离液在所述管体底部，所述分离胶体的密度小于所述分离液位于所述分离液上方；在25°C时，所述分离胶体的密度为1.0655-1.0755g/cm