factor

摘要： Each nation has its own unique national characters.British are known as conservative,gentlemanlike,reticent,etc.While Americans are thought to be independent,outgoing,optimistic and democratic.A comparative study of the geographical and historical factors of these two countries will objectively cast a light on the differences of their national characters.

摘要： The goal of the present study is to develop a more suitable inventory to evaluate the burnout of non-English majors in China,which we called it foreign language learning burnout inventory(FLLB).The operative definition of burnout proposed by Maslach and Jackson is used to define three dimensions(exhaustion,cynicism and reduced efficacy).The selection of items is based on the existing burnout inventories,combining with the consideration of the features showed in the studies of foreign language learning.In the research,the data obtained from 101 non-English majors and is analyzed for the validity and reliability studies studies of FLLB.Three factors explained 56%of the total variance.Factor loadings ranged from.438 to.742.Cronbach Alpha reliability coefficient for the sub-dimensions ranged from.861 to.915.The model indices emerged from the Confirmtory Factor Analysis is[GFI=.994,RMSEA=.067,[X^(2)=458.086,df=206,p<0.01]]indicated that there was a good fit.

摘要： BACKGROUND Variations in the methylene tetrahydrofolate reductase(MTHFR)gene have been reported as risk factors for numerous conditions,including cardiovascular disease,thrombophilia,stroke,hypertension and pregnancy-related complications.Moreover,it was reported there is an association between breast cancer and mutations in MTHFR-C677T.However,whether there is an association between MTHFR gene polymorphism and granulomatous lobular mastitis or not has been rarely investigated.AIM To analyze the association between MTHFR gene polymorphism and granulomatous lobular mastitis.METHODS Fifty-one patients with granulomatous lobular mastitis admitted to The First Hospital of Kunming were selected as study samples.Their hospitalization time ranged from February 2018 to February 2019.The 51 patients were included in the experimental group,and another 51 women who underwent physical examination at The First Hospital of Kunming in the same period were included in the control group.Deoxyribonucleic acid and MTFR genetic polymorphism testing were performed in each group.The association between MTHFR gene polymorphism and granulomatous lobular mastitis was observed.RESULTS There were significant differences in genotype frequency and allele frequency of C/C and C/T between the experimental group and the control group(all P0.05).In addition,there was no significant difference in genotype frequency and allele frequency of A/A,A/C and C/C between the two groups(P>0.05).CONCLUSION MTHFR gene C677T locus polymorphism is closely related to granulomatous lobular mastitis.

摘要： Beta-nerve growth factor(β-NGF)is known to be a major leading cause of neuronal plasticity.To identify the possible action mechanisms ofβ-NGF gene therapy for sciatic nerve recovery,experimental dogs were randomly divided into control,pyridoxine,and pyridoxine+β-NGF groups.We observed chronological changes of morphology in the dorsal root ganglia in response to pyridoxine toxicity based on cresyl violet staining.The number of large neurons positive for cresyl violet was dramatically decreased after pyridoxine intoxication for 7 days in the dorsal root ganglia and the neuron number was gradually increased after pyridoxine withdrawal.In addition,we also investigated the effects ofβ-NGF gene therapy on neuronal plasticity in pyridoxine-induced neuropathic dogs.To accomplish this,tyrosine kinase receptor A(TrkA),βIII-tubulin and doublecortin(DCX)immunohistochemical staining was performed at 3 days after the last pyridoxine treatment.TrkA-immunoreactive neurons were dramatically decreased in the pyridoxine group compared to the control group,but strong TrkA immunoreactivity was observed in the small-sized dorsal root ganglia in this group.TrkA immunoreactivity in the dorsal root ganglia was similar betweenβ-NGF and control groups.The numbers ofβⅢ-tubulin-and DCX-immunoreactive cells decreased significantly in the pyridoxine group compared to the control group.However,the reduction ofβⅢ-tubulin-and DCX-immunoreactive cells in the dorsal root ganglia in theβ-NGF group was significantly ameliorated than that in the pyridoxine group.These results indicate thatβ-NGF gene therapy is a powerful treatment of pyridoxine-induced neuropathic damage by increasing the TrkA and DCX levels in the dorsal root ganglia.The experimental protocol was approved by the Institutional Animal Care and Use Committee(IACUC)of Seoul National University,South Korea(approval No.SNU-060623-1,SNU-091009-1)on June 23,2006 and October 9,2009,respectively.

摘要： Viruses are representative of a global threat to agricultural production. Genetic resistance is the preferred strategy for the control of viral infection and against loss of crop yield. Viral protein synthesis requires host cellular factors for translating their viral RNAs, and for regulating their replication and cell to cell systemic movement. Therefore, the viruses are dependent on cellular translation factors. Mutations in the gene encoding eIF4E and eIF4G or their isoforms, eIFiso4 E, eIFiso4 G and eIF2Bβ have been mapped as a source of plant potyvirus while other genus of plant virus recessive resistance genes in many species are originated from these loci. Some of other plant translation factors, such as eIF3,eIF4 A-like helicases, eEF1A and eEF1B, which are required in interacting with viral RNAs and regulating various aspects of the infection cycle,have also been identified. Here, we summarized the mechanisms utilized by RNA viruses of eukaryotic plants and the essential roles of e IFs in virus infection. Moreover, we discussed the potential of e IFs as a target gene in the development of genetic resistance to viruses for crop improvement. This review highlighted newly revealed examples of abnormal translational strategies and provided insights into natural host resistance mechanisms that have been linked to 3 cap-independent translational enhancer activity.

摘要： In recent years, as the enrollment rate of Chinese colleges has increased year by year, the identification of needy undergraduates has become increasingly important. However, the traditional way to identify college students with financial difficulties mainly relies on manual review and collective voting, which easily causes subjectivity and randomness. To alleviate the problem above, this paper establishes an automatic identification model for needy undergraduates based on the 1842 questionnaires collected from undergraduates in WHUT. Firstly, this paper filters the questionnaire preliminary using the local outlier factor algorithm. Secondly, this paper combines mutual information, Spearman rank correlation coefficient and distance correlation coefficient by rank-sum ratio to select features for eliminating noise from irrelevant features. Thirdly, this paper trains filed-aware factor machine model and compares it with other models, such as Logistic Regression, SVM, etc. Eventually, this paper finds that filed-aware factor machine performers much better than other models in the identification of needy undergraduates, and prominent features affecting the identification of needy undergraduates are the year of the family income, cost of living provided parents, etc.

摘要： BACKGROUND Pancreatic cancer(PC)is a leading cause of cancer related mortality worldwide,with poor survival due to late diagnosis.Currently,biomarkers have limited use in early diagnosis of PC.Macrophage inhibitory cytokine-1 or growth differentiation factor-15(MIC-1/GDF15)has been implicated as a potential serum biomarker in PC and other malignancies.AIM To determine the role of MIC-1/GDF15 in detecting pre-malignant pancreatic lesions and neoplastic tumours in an asymptomatic high-risk cohort part of Australian Pancreatic Cancer Screening Program.METHODS A feasibility prospective single centre cohort study was performed.Participants recruited for yearly surveillance with endoscopic ultrasound(EUS)had serial fasting blood samples collected before EUS for MIC-1/GDF15,C-reactive protein and carbohydrate antigen 19-9.Patients were stratified into five groups based on EUS findings:Normal;pancreatic cysts,branch-duct intraductal papillary mucinous neoplasm;diffuse non-specific abnormalities;and neoplastic tumours.MIC-1/GDF15 serum levels were quantified using ELISA.Participants in whom EUS demonstrated abnormalities but not malignancy were closely followed up with magnetic resonance imaging(MRI)or computed tomography.RESULTS One hundred twenty participants were prospectively recruited from 2011-2018.Forty-seven participants(39.2%)had an abnormal EUS and five participants(4.2%)were diagnosed with neoplastic tumours,three by EUS(two pancreatic and one liver)and two by MRI/computed tomography(breast cancer,bladder cancer),which were performed for follow up of abnormal EUS.Baseline serum MIC-1/GDF15 was a significant predictor of neoplastic tumours on receiver operator characteristic curve analysis[area under curve(AUC)=0.814,P=0.023].Baseline serum MIC-1/GDF15 had moderate predictive capacity for branch-duct intraductal papillary mucinous neoplasm(AUC=0.644)and neoplastic tumours noted on EUS(AUC=0.793),however this was not significant(P=0.188 and 0.081 respectively).Serial serum MIC-1/GDF15 did not demonstrate a significant percentage change between a normal and abnormal EUS(P=0.213).Median baseline MIC-1/GDF15 was greater in those with neoplastic tumours(Median=1039.6,interquartile range=727.0-1977.7)compared to those diagnosed with a benign lesion(Median=570.1,interquartile range=460.7-865.2)on EUS and MRI(P=0.012).CONCLUSION In this pilot study MIC-1/GDF15 has predictive capacity for neoplastic tumours in asymptomatic individuals with a genetic predisposition for PC.Further imagining may be warranted in patients with abnormal EUS and raised serum MIC-1/GDF15.Larger multicentric prospective studies are required to further define the role of MIC-1/GDF15 as a serological biomarker in pre-malignant pancreatic lesions and neoplastic tumours.

摘要： BACKGROUND Colorectal cancer(CRC)is one of the most prevalent tumors worldwide.Recently,long noncoding RNAs(lncRNAs)have been shown to influence tumorigenesis and tumor progression by acting as competing endogenous RNAs(ceRNAs).It is difficult to extract prognostic lncRNAs and useful bioinformation from most ceRNA networks constructed previously.AIM To construct a prognostic related ceRNA regulatory network and lncRNA related signature based on risk score in CRC.METHODS RNA transcriptome profile and clinical information of 506 CRC patients were downloaded from the Cancer Genome Atlas database.R packages and Perl program were used for data processing.Cox regression analysis was used for prognostic model construction.Quantitative real-time polymerase chain reaction was used to detect the expression of lncRNAs.RESULTS A prognostic-related ceRNA network was constructed,including 9 lncRNAs,44 mRNAs,and 30 miRNAs.In addition,a four-lncRNA model was constructed using multivariate Cox regression analysis,which could be an independent prognostic model in CRC.The risk score for each patient was calculated,and the 506 patients were divided into high and low-risk groups(253 for each group)based on the median risk score.The results of the survival analysis showed that patients with a high-risk score had a poor survival rate.Furthermore,the predictive value of the four-lncRNA model was evaluated in GSE38832.Patient survival probabilities could be better predicted when combing the risk score and clinical features.Gene Set Enrichment Analysis results verified that a number of cancer-related signaling pathways were enriched with a high-risk score in CRC.Finally,we validated a novel lncRNA(LINC00488)using quantitative real-time polymerase chain reaction in 22 paired CRC patient tumor tissues compared to adjacent non-tumor tissues.CONCLUSION The four-lncRNA model could give better predictive value for CRC patients.Our understanding of the lncRNA-related ceRNA regulatory mechanism could provide a potential diagnostic indicator for CRC patients.

摘要： Zebrafish and human genomes are highly homologous;however,despite this genomic similarity,adult zebrafish can achieve neuronal proliferation,regeneration and functional restoration within 6–8 weeks after spinal cord injury,whereas humans cannot.To analyze differentially expressed zebrafish genes between axon-regenerated neurons and axon-non-regenerated neurons after spinal cord injury,and to explore the key genes and pathways of axonal regeneration after spinal cord injury,microarray GSE56842 was analyzed using the online tool,GEO2R,in the Gene Expression Omnibus database.Gene ontology and protein-protein interaction networks were used to analyze the identified differentially expressed genes.Finally,we screened for genes and pathways that may play a role in spinal cord injury repair in zebrafish and mammals.A total of 636 differentially expressed genes were obtained,including 255 up-regulated and 381 down-regulated differentially expressed genes in axon-regenerated neurons.Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment results were also obtained.A protein-protein interaction network contained 480 node genes and 1976 node connections.We also obtained the 10 hub genes with the highest correlation and the two modules with the highest score.The results showed that spectrin may promote axonal regeneration after spinal cord injury in zebrafish.Transforming growth factor beta signaling may inhibit repair after spinal cord injury in zebrafish.Focal adhesion or tight junctions may play an important role in the migration and proliferation of some cells,such as Schwann cells or neural progenitor cells,after spinal cord injury in zebrafish.Bioinformatic analysis identified key candidate genes and pathways in axonal regeneration after spinal cord injury in zebrafish,providing targets for treatment of spinal cord injury in mammals.

摘要： 本发明公开了一种Factor II和Factor V基因多态性检测特异性引物和液相芯片，该液相芯片主要包括有：每种由5’端的tag序列和3’端针对目的基因突变位点的特异性引物序列组成的ASPE引物，所述特异性引物序列为：针对Factor II基因A140G位点的SEQ ID NO.7及SEQ ID NO.8；针对Factor II基因G78A位点的SEQ ID NO.9及SEQ ID NO.10；和/或针对FactorV基因G125A位点的SEQ ID NO.11及SEQ ID NO.12；有anti-tag序列包被的微球；扩增引物。本发明所提供的检测液相芯片的检测结果与测序法的吻合率高达100％，实现多个突变位点的野生型和突变型并行检测。

摘要： 本申请实施例公开了一种计算Q‑Factor的电路、方法、装置及电子设备，用于提高Q‑Factor的精确度。本申请实施例为：滤波电路、谐振网络、第一检测电路和控制电路；滤波电路包括第一电阻R1、第二电阻R2、第三电阻R3、第一电容C1、第二电容C2、放大器和比较器；谐振网络分别与控制电路和第一电阻R1第一端连接，第一电阻R1的第二端与放大器连接；放大器分别与第二电阻R2第一端和第三电阻R3第一端连接；第二电阻R2第二端分别与第一电容C1的第一端和比较器第一输入口连接；第三电阻R3第二端分别与第一电容C1第二端、第二电容C2第一端以及比较器第二输入口连接；第二电容C2第二端接地；比较器与第一检测电路连接；第一检测电路与控制电路连接。

摘要： 本发明公开了一种Factor II和Factor V基因多态性检测特异性引物和液相芯片，该液相芯片主要包括有：每种由5’端的tag序列和3’端针对目的基因突变位点的特异性引物序列组成的ASPE引物，所述特异性引物序列为：针对Factor II基因A140G位点的SEQ ID NO.7及SEQ ID NO.8；针对Factor II基因G78A位点的SEQ ID NO.9及SEQ ID NO.10；和/或针对FactorV基因G125A位点的SEQ ID NO.11及SEQ ID NO.12；有anti-tag序列包被的微球；扩增引物。本发明所提供的检测液相芯片的检测结果与测序法的吻合率高达100％，实现多个突变位点的野生型和突变型并行检测。

摘要： 本申请实施例公开了一种计算Q‑Factor的电路、方法、装置及电子设备，用于提高Q‑Factor的精确度。本申请实施例为：滤波电路、谐振网络、第一检测电路和控制电路；滤波电路包括第一电阻R1、第二电阻R2、第三电阻R3、第一电容C1、第二电容C2、放大器和比较器；谐振网络分别与控制电路和第一电阻R1第一端连接，第一电阻R1的第二端与放大器连接；放大器分别与第二电阻R2第一端和第三电阻R3第一端连接；第二电阻R2第二端分别与第一电容C1的第一端和比较器第一输入口连接；第三电阻R3第二端分别与第一电容C1第二端、第二电容C2第一端以及比较器第二输入口连接；第二电容C2第二端接地；比较器与第一检测电路连接；第一检测电路与控制电路连接。

摘要： 本实用新型涉及变压器技术领域，具体涉及一种K‑FACTOR变压器的铁芯结构，包括第一芯柱、第二芯柱、第三芯柱、上铁轭以及下铁轭；所述铁芯结构还包括上夹板以及下夹板；所述上夹板通过第一螺丝将上夹板、第一卡块以及第一卡槽固定；所述下夹板通过第二螺丝将下夹板、第二卡块以及第二卡槽固定；所述下夹板设有固定座；所述上铁轭的顶部设有U形压件；所述固定座与U形压件之间设有对拉螺杆。本实用新型通过上下夹板和对拉螺杆共同作用实现了对芯柱水平方向和竖直方向上的夹持固定，并且上下夹板、对拉螺杆与芯柱之间都是通过螺丝锁接的，没有焊接过程，螺丝锁接易于实现自动化生产，生产效率高。

摘要： 本实用新型涉及变压器技术领域，具体涉及一种K‑FACTOR变压器，包括芯柱以及上铁轭、下铁轭；所述芯柱的表面设有次级线圈；所述次级线圈的表面设有初级线圈；所述静电屏蔽层包括第一屏蔽层、第二屏蔽层、第三屏蔽层以及第四屏蔽层；所述K‑FACTOR变压器还包括用于固定芯柱、上铁轭以及下铁轭的垂直固定机构；所述K‑FACTOR变压器还包括用于固定上铁轭以及下铁轭的水平固定机构。本实用新型通过设置第一屏蔽层、第二屏蔽层、第三屏蔽层以及第四屏蔽层，使用多层屏蔽结构，能够有效地减少谐波损耗对变压器的影响，另外，通过垂直固定机构以及水平固定机构能够使得芯柱、上铁轭以及下铁轭之间的连接更稳稳固，防止变压器震动。

摘要： 本发明公开了灰飞虱致死基因片段Transcription factor IIB及其应用。本发明筛选出经干扰后能够导致灰飞虱死亡的序列如SEQ ID NO.1所示的灰飞虱致死基因片段Transcription factor IIB，利用该基因片段的dsRNA饲喂灰飞虱、褐飞虱，可有效地致死灰飞虱、褐飞虱，本发明对环境生态和食品都安全，为利用RNA干扰技术控制害虫提供了新途径。

摘要： 为了解决现有技术中测试工装测试误差大的问题，本公开提供了一种针对Pin‑Fin功率模块K‑factor的测试工装及方法，提高测试精度。包括液冷夹具本体和温控设备，温控设备连接有温度传感器，温度传感器包括螺纹部和检测部；液冷夹具本体处设有与温度传感器螺纹部配合的螺纹孔，用于液冷夹具本体与Pin‑Fin功率模块装配且温度传感器安装到螺纹孔后，温度传感器的检测部插入Pin‑Fin缝隙且紧挨Pin‑Fin功率模块的基板。本公开的技术方案中，测试工装可以检测基于稳态下的基板壳温，检测得到的基板壳温比现有技术中的温控设备内部油温更能反映Pin‑Fin功率模块芯片的温度，提高了芯片结温的测试精度，基于该基板壳温可以得出的K‑factor的精确度更高。

摘要： 本发明公开了灰飞虱致死基因片段Transcription factor IIB及其应用。本发明筛选出经干扰后能够导致灰飞虱死亡的序列如SEQ ID NO.1所示的灰飞虱致死基因片段Transcription factor IIB，利用该基因片段的dsRNA饲喂灰飞虱、褐飞虱，可有效地致死灰飞虱、褐飞虱，本发明对环境生态和食品都安全，为利用RNA干扰技术控制害虫提供了新途径。

摘要： 本发明公开了优化的Komagataella pastoris酵母α‑factor信号肽及应用。本发明对具有如SEQID NO:1所示氨基酸序列的Komagataella pastoris酵母α‑factor信号肽引入一个N糖基化位点，得到优化后的Komagataella pastoris酵母α‑factor信号肽，其氨基酸序列如SEQIDNO:3所示。本发明优化的α‑factor信号肽特别适用于引导外源蛋白在毕赤酵母中的分泌表达，与优化前信号肽相比，表达水平提高了25‑35倍，其应用有利于筛选到高表达菌株，具有降低生产成本、便于后续纯化等优势。