regeneration

摘要： Exfoliated deciduous or an extracted healthy adult tooth can be used to harvest,process,and cryogenically preserve dental pulp stem cells.Future stem cell-based regenerative medicine methods could benefit significantly from these mesenchymal stem cells.Teeth serve as a substantial source of mesenchymal stem cells,otherwise disposed of as medical waste.Care should be taken to store this treasure trove of stem cells.Collective responsibility of patients,dentists,and physicians is necessary to ensure that this valuable resource is not wasted and that every possible dental pulp stem cell is available for use in the future.The dental pulp stem cells(DPSC)inside teeth represent a significant future source of stem cells for regenerative medicine procedures.This review describes the ontogeny,the laboratory processing and collection,and isolation methods of DPSC.This review also discusses currently available stem cell banking facilities and their potential use in regenerative medicine procedures in dental and general medical applications in the future.

摘要： The onset of retinal degenerative disease is often associated with neuronal loss. Therefore, how to regenerate new neurons to restore vision is an important issue. NeuroD1 is a neural transcription factor with the ability to reprogram brain astrocytes into neurons in vivo. Here, we demonstrate that in adult mice, NeuroD1 can reprogram Müller cells, the principal glial cell type in the retina, to become retinal neurons. Most strikingly, ectopic expression of NeuroD1 using two different viral vectors converted Müller cells into different cell types. Specifically, AAV7 m8 GFAP681::GFP-ND1 converted Müller cells into inner retinal neurons, including amacrine cells and ganglion cells. In contrast, AAV9 GFAP104::ND1-GFP converted Müller cells into outer retinal neurons such as photoreceptors and horizontal cells, with higher conversion efficiency. Furthermore, we demonstrate that Müller cell conversion induced by AAV9 GFAP104::ND1-GFP displayed clear dose-and time-dependence. These results indicate that Müller cells in adult mice are highly plastic and can be reprogrammed into various subtypes of retinal neurons.

摘要： Neuroma formation after peripheral nerve transection often leads to severe neuropathic pain.Regenerative peripheral nerve interface has been shown to reduce painful neuroma in the clinic.However,no reports have investigated the underlying mechanisms,and no comparative animal studies on regenerative peripheral nerve interface and other means of neuroma prevention have been conducted to date.In this study,we established a rat model of left sciatic nerve transfection,and subsequently interfered with the model using the regenerative peripheral nerve interface or proximal nerve stump implantation inside a fully innervated muscle.Results showed that,compared with rats subjected to nerve stump implantation inside the muscle,rats subjected to regenerative peripheral nerve interface intervention showed greater inhibition of the proliferation of collagenous fibers and irregular regenerated axons,lower expressions of the fibrosis markerα-smooth muscle actin and the inflammatory marker sigma-1 receptor in the proximal nerve stump,lower autophagy behaviors,lower expressions of c-fos and substance P,higher expression of glial cell line-derived neurotrophic factor in the ipsilateral dorsal root ganglia.These findings suggested that regenerative peripheral nerve interface inhibits peripheral nerve injury-induced neuroma formation and neuropathic pain possibly via the upregulation of the expression of glial cell line-derived neurotrophic factor in the dorsal root ganglia and reducing neuroinflammation in the nerve stump.

摘要： At the request of the authors,the Editor of Neural Regeneration Research(NRR)has retracted the publication entitled“Various changes in cryopreserved acellular nerve allografts at−80°C”(Huang et al.,2018;doi:10.4103/1673-5374.237138)following the concerns of duplicate publication Image B in graphical abstract and Figure 1 in body text appear the same as the images published in Chin J Microsurg(Zhu et al.,2016).

摘要： The Editors of Neural Regeneration Research have retracted the publication entitled“Emerging concepts underlying selective neuromuscular dysfunction in infantile-onset spinal muscular atrophy”(Gollapalli et al.,2021;doi:10.4103/1673-5374.308073)following concerns stemming from misquoting and,accordingly,faulty characterization of key literature cited.

摘要： In the article titled“Brain-derived neurotrophic factor and its related enzymes and receptors play important roles after hypoxic-ischemic brain damage”,published on pages 1453-1459,Issue 8,Volume 16 of Neural Regeneration Research(Xiong et al.,2021),there are some errors in author list and affiliations because of mistake in description and also oversight during final proof checking,the correct author list and affiliations are shown below.

摘要： While the anatomical properties of regenerated axons across spinal cord lesion sites have been studied extensively,little is known of how the functional properties of regenerated synapses compared to those in unlesioned animals.This study aims to compare the properties of synapses made by regenerated axons with unlesioned axons using the lamprey,a model system for spinal injury research,in which functional locomotor recovery after spinal cord lesions is associated with axonal regeneration across the lesion site.Regenerated synapses below the lesion site did not differ from synapses from unlesioned axons with respect to the amplitude and duration of single excitatory postsynaptic potentials.They also showed the same activity-dependent depression over spike trains.However,regenerated synapses did differ from unlesioned synapses as the estimated number of synaptic vesicles was greater and there was evidence for increased postsynaptic quantal amplitude.For axons above the lesion site,the amplitude and duration of single synaptic inputs also did not differ significantly from unlesioned animals.However,in this case,there was evidence of a reduction in release probability and inputs facilitated rather than depressed over spike trains.Synaptic inputs from single regenerated axons below the lesion site thus do not increase in amplitude to compensate for the reduced number of descending axons after functional recovery.However,the postsynaptic input was maintained at the unlesioned level using different synaptic properties.Conversely,the facilitation from the same initial amplitude above the lesion site made the synaptic input over spike trains functionally stronger.This may help to increase propriospinal activity across the lesion site to compensate for the lesion-induced reduction in supraspinal inputs.The animal experiments were approved by the Animal Ethics Committee of Cambridge University.

摘要： Pt-based catalysts are widely used in propane dehydrogenation reaction for the production of propylene.Suppressing irreversible deactivation caused by the sintering of Pt particles under harsh conditions and regeneration process is a significant challenge in this catalyst.Herein,a series of highly ordered mesoporous Al_(2)O_(3) supports with different levels of Al3+penta sites,are fabricated and used as the support to disperse Pt-Sn_(2) clusters.Characterizations of Pt-Sn_(2)/meso-Al_(2)O_(3) with XRD,NMR,CO-IR,STEM,TG,and Raman techniques along with propane dehydrogenation-regeneration cycles test reveal the structure-stability-re generability relationship.The coordinatively unsaturated pentacoordinate Al_(Al3+penta)^(3+)can strongly anchor Pt atoms via a formation of Al-O-Pt bond,and thus stabilize the Pt-based particles at the surface of Al_(2)O_(3).The stability and regenerability of Pt-Sn2/meso-Al_(2)O_(3) are strongly dependent on the content of Al3+penta sites in the Al_(2)O_(3) structure,and a high level of Al3+penta sites can effectively prevent the agglomeration of Pt-Sn2 clusters into large Pt nanoparticles in the consecutive dehydrogenation-regeneration cycles.The Pt-Sn2/meso-Al_(2)O_(3)-600 with the highest level of Al_(penta)^(3+) (50.8%)delivers the best performance in propane dehydrogenation,which exhibits propane conversion of 40%and propylene selectivity above 98%at 570°C with 10 vol%C_(3)H_(8) and 10 vol% H_(2) feed.A slow deactivation in this catalyst is ascribed to the formation of coke,and the catalytic performance can be fully restored in the consecutive dehydrogenation-regeneration cycles via a simple calcination treatment.

摘要： Previous studies showed that acetyl-11-keto-beta-boswellic acid(AKBA),the active ingredient in the natural Chinese medicine Boswellia,can stimulate sciatic nerve injury repair via promoting Schwann cell proliferation.However,the underlying molecular mechanism remains poorly understood.In this study,we performed genomic sequencing in a rat model of sciatic nerve crush injury after gastric AKBA administration for 30 days.We found that the phagosome pathway was related to AKBA treatment,and brain-derived neurotrophic factor expression in the neurotrophic factor signaling pathway was also highly up-regulated.We further investigated gene and protein expression changes in the phagosome pathway and neurotrophic factor signaling pathway.Myeloperoxidase expression in the phagosome pathway was markedly decreased,and brain-derived neurotrophic factor,nerve growth factor,and nerve growth factor receptor expression levels in the neurotrophic factor signaling pathway were greatly increased.Additionally,expression levels of the inflammatory factors CD68,interleukin-1β,pro-interleukin-1β,and tumor necrosis factor-αwere also decreased.Myelin basic protein-andβ3-tubulin-positive expression as well as the axon diameter-to-total nerve diameter ratio in the injured sciatic nerve were also increased.These findings suggest that,at the molecular level,AKBA can increase neurotrophic factor expression through inhibiting myeloperoxidase expression and reducing inflammatory reactions,which could promote myelin sheath and axon regeneration in the injured sciatic nerve.

摘要： Background:In this investigation,we explore the literature regarding neuroregeneration from the 1700s to the present.The regeneration of central nervous system neurons or the regeneration of axons from cell bodies and their reconnection with other neurons remains a major hurdle.Injuries relating to war and accidents attracted medical professionals throughout early history to regenerate and reconnect nerves.Early literature till 1990 lacked specific molecular details and is likely provide some clues to conditions that promoted neuron and/or axon regeneration.This is an avenue for the application of natural language processing(NLP)to gain actionable intelligence.Post 1990 period saw an explosion of all molecular details.With the advent of genomic,transcriptomics,proteomics,and other omics-there is an emergence of big data sets and is another rich area for application of NLP.How the neuron and/or axon regeneration related keywords have changed over the years is a first step towards this endeavor.Methods:Specifically,this article curates over 600 published works in the field of neuroregeneration.We then apply a dynamic topic modeling algorithm based on the Latent Dirichlet allocation(LDA)algorithm to assess how topics cluster based on topics.Results:Based on how documents are assigned to topics,we then build a recommendation engine to assist researchers to access domain-specific literature based on how their search text matches to recommended document topics.The interface further includes interactive topic visualizations for researchers to understand how topics grow closer and further apart,and how intra-topic composition changes over time.Conclusions:We present a recommendation engine and interactive interface that enables dynamic topic modeling for neuronal regeneration.

摘要： The regeneration of CoNiAl calcined hydrotalcite-like compounds for hydrolysis of COS wasstudied. The catalytic activity could be recovered after immersing Na2CO3solution and calcining the catalystunder air flow at 350°C.The fresh, deactivated and regenerated catalysts were characterized by varioustechniques. The presence of residual alkaline ions after regeneration is the main source for the strong basicity.On one hand the retained sodium introduced in the regeneration and NiO play as active sites for COShydrolysis. On the other hand, the retained sodium enhances the basicity, increasing the catalytic activity. Thesurface area of catalyst recovered and the pore size at the range of 41.51-74.52 increased after three cyclesof regeneration.

摘要： 利用选择性再生来提高液压回路的可控性和效率的系统和方法。当该功能在空气中并且存在气蚀作用的风险时候，或者当该功能做正功并且需要有效率的时候，再生失活阀能够对压差做出反应。当该功能存在气蚀作用的风险的时候，该再生失活阀能够对潜在的气蚀作用做出反应，并且该再生失活阀关闭以便该功能再生。当该功能不存在气蚀风险时候，再生失活阀同样能够做出反应，并且能够打开使得该功能以更大的功率和更高的效率来移动。

摘要： 本发明公开了高羊茅“Regenerate”FaHsfC1b基因及其应用。本发明从高羊茅“Regenerate”中克隆出基因FaHsfC1b并利用过表达的方式鉴别了其功能。核苷酸序列如SEQ ID NO.3所示。FaHsfC1b具有热激转录因子的典型特征，包括一个DNA结合区域，一个疏水的寡聚化结构域，一个核定位信号。在拟南芥中过表达FaHsfC1b，结果表明FaHsfC1b转基因拟南芥具有比野生型拟南芥更强的抗热能力，同时也提高了热相关下游基因的表达。说明FaHsfC1b基因具有提高植物耐热性的功能。

摘要： 利用选择性再生来提高液压回路的可控性和效率的系统和方法。当该功能在空气中并且存在气蚀作用的风险时候，或者当该功能做正功并且需要有效率的时候，再生失活阀能够对压差做出反应。当该功能存在气蚀作用的风险的时候，该再生失活阀能够对潜在的气蚀作用做出反应，并且该再生失活阀关闭以便该功能再生。当该功能不存在气蚀风险时候，再生失活阀同样能够做出反应，并且能够打开使得该功能以更大的功率和更高的效率来移动。