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钠泵

钠泵的相关文献在1986年到2022年内共计138篇,主要集中在内科学、基础医学、药学 等领域,其中期刊论文107篇、会议论文5篇、专利文献26篇;相关期刊84种,包括中国应用生理学杂志、中华心血管病杂志、中国药理学通报等; 相关会议5种,包括中国医师协会输血分会2012年第六届输血学术年会、第六届全国新堆与研究堆学术会议、第十二届全国反应堆结构力学会议等;钠泵的相关文献由320位作者贡献,包括吕卓人、张明娟、王颢等。

钠泵—发文量

期刊论文>

论文:107 占比:77.54%

会议论文>

论文:5 占比:3.62%

专利文献>

论文:26 占比:18.84%

总计:138篇

钠泵—发文趋势图

钠泵

-研究学者

  • 吕卓人
  • 张明娟
  • 王颢
  • 陆金琪
  • 原卫清
  • 毛裕民
  • 谢毅
  • 刘红
  • 张慧峰
  • 李玉明
  • 期刊论文
  • 会议论文
  • 专利文献

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    • 浦恩山; 谷继品
    • 摘要: 快堆钠泵是快堆的关键设备,其能否安全可靠运行对反应堆的安全极为重要,因此,其可靠性设计至关重要。钠泵可靠性分配是钠泵可靠性设计中关键的内容,本文利用评分分配法将钠泵的可靠性指标进行了分配,分配过程中充分考虑钠泵各部件的复杂程度、技术水平、工作时间及环境条件四个因素,并对评分的结果进行了正态性检验和可信性评估,进一步提高了可靠性分配的准确性。通过可靠性分配,钠泵各部件的设计人员明确了其可靠性设计要求,同时也为其确定技术方案提供了依据。
    • 夏宇; 刘兆阳; 孙刚; 邓夏; 孙帅; 王明政; 吴水金
    • 摘要: 压力管由三通、支管和密封组件等组成,用于连接一回路钠泵出口与反应堆大栅板联箱,为冷钠提供流道。堆容器研发团队根据CFR600示范快堆整堆的功能、指标和工况,分解出压力管部件的功能、指标和工况,初步设计压力管方案图;根据压力管所受温度、载荷、介质、辐照等条件,选定材料开展压力管失效模式分析。
    • 张杰; 何小冬; 延方泉; 牛红军; 孙德臣; 张鑫太
    • 摘要: 二回路钠泵采用的是立式、单级机械钠泵,它与电磁泵相比效率更高。叶轮、导叶、压出室作为泵的核心水力部件,对泵效率的影响至关重要。本文通过计算泵的比转速,选取优秀的水力模型,采用CFD对流体流动进行数值模拟,使用NUMECA软件进行分析。预测选取的模型是否满足性能要求,最后通过试验验证,对比前期数值分析结果,达到预期水力性能要求,同时也验证了模型数值分析方法可行。
    • 王海华; 王海珍; 史娜; 王竹青; 王耀军; 周萍萍; 王静
    • 摘要: 目的:探讨钠泵活性改变及内质网应激(ERS)在大鼠离体心脏再灌损伤中的作用及其机制.方法:将60只雄性SD大鼠随机分为6组(n=10):正常对照组(NC组)、缺血/再灌损伤组(I/R组)、哇巴因-缺血/再灌损伤组(OUA-I/R组)、地高辛抗血清-缺血/再灌损伤组(Anti-Dig-I/R组)、Src抑制剂PP2-哇巴因-缺血/再灌损伤组(PP2-OUA-I/R组)、PLC抑制剂U73122-哇巴因-缺血/再灌损伤组(U73122-OUA-I/R组).建立全心缺血30 min,再灌注120min的Langendorff大鼠离体心脏缺血再灌损伤模型.检测各组相同时间点心功能恢复率、冠脉流出液中乳酸脱氢酶(LDH)和肌酸激酶(CK)活性,心肌中Na+-K+-ATP酶活性和钙离子水平.流式细胞仪检测心肌细胞凋亡率,Westem blot检测心肌钠泵α1亚基、葡萄糖调节蛋白78(GRP78)、C/EBP同源蛋白(CHOP)及凋亡蛋白Bcl-2/Bax的表达.结果:与I/R组相比,给予哇巴因预处理可使心功能恢复率明显下降,心肌酶漏出增多,Na+-K+-ATP酶的活性降低,心肌细胞内钙水平升高,细胞凋亡率增多,心肌钠泵α1亚基和Bcl-2表达降低,GRP78、CHOP和Bax表达升高;而Anti-Dig-I/R组与I/R组相比各指标均明显改善;给予Src抑制剂PP2或PLC抑制剂U73122后,哇巴因对心肌的损伤作用被部分阻断,表现为心功能恢复率升高,心肌酶漏出减少,Na+-K+-ATP酶的活性明显恢复,Ca2+水平下降,细胞凋亡率下降,心肌钠泵α1亚基和Bcl-2表达增多,GRP78和Bax表达减少.结论:钠泵功能改变和内质网应激共同参与大鼠离体心脏缺血再灌损伤,钠泵通路(Src和PLC)介导内质网应激是引起大鼠离体心脏缺血再灌损伤细胞凋亡机制之一.
    • 王海燕; 郝亮; 郭宇松; 王永利; 郭炜
    • 摘要: Aim To explore the effects of hypertension on the contractibility of rat basilar artery and its inter-actions with the sodium pump activity.Methods The basilar artery was respectively isolated from Wistar and SHR rats,and the isometric tension of arterial rings was measured by Multi Myograph System-610M.The cont-ractibilities of arterial rings induced by KCl or 5-HT were compared between the basilar arteries of the two groups of rats to analyze the effect of hypertension on the cerebral vascular tension and the activity of sodium pump.Results In SHR rats,the concentration-re-sponse curves of the contraction of isolated basilar ar-tery rings induced by KCl and 5-HT were significantly shifted to right,and the relaxation of vascular tone in-duced by K+which was reintroduced from the external was attenuated compared with those in the WR.These results suggested that hypertension could significantly decrease the activity of the sodium pump and the con-tractile responses of KCl and 5-HT.OUA could con-tract the basilar artery in a concentration-dependent manner,and its concentration-response curve was opti-mally fitted by a two-site binding model:Kd was 1.7 ×10 -8 and 1.6 ×10 -5 mol·L-1,respectively.The results indicated that the two different function sodium pumps existed in the rat basilar artery:one with the high OUA affinity and the other with the low OUA af-finity.If the high and low affinity sodium pumps were inhibited by 5 ×10 -7 and 10 -4 mol · L-1 OUA,re-spectively,the concentration-response curves of KCl and 5-HT would shift to left in SHR rats but not in WR rats.It suggested that OUA could enhance the contrac-tion induced by KCl and 5-HT significantly,and a concentration-dependent effect was observed in the SHR vascular contraction induced by 5-HT (r =0.9393 ,P<0.05 ).When the two concentrations of OUA were applied,there was no significant difference in the shift left of the concentration-response curves in-duced by KCl in the SHR cerebral vessels.However, the marked difference was shown in the shift left in-duced by 5-HT.The results implied that only the high affinity sodium pump was involved in the contractile re-sponse of SHR cerebral vascular to KCl,whereas,the contractile response of SHR cerebral vascular to 5-HT was induced by both high and low affinity sodium pumps.Conclusion Hypertension could lower the contractile response of the basilar artery to vasocon-strictors,and the mechanism might relate to the de-creased sensitivity of the sodium pump induced by hy-pertension or the increased sensibility of the sodium pump to OUA.%目的:探讨高血压对大鼠脑血管收缩性的影响及其与钠泵活性的关系。方法取♂ Wistar大鼠(WR)和自发性高血压大鼠(SHR)基底动脉环,通过Multi Myograph张力换能系统记录血管张力变化,比较两种大鼠离体脑动脉对KCl和5-羟色胺(5-HT)的收缩反应,分析高血压病变对脑血管收缩性和钠泵活性的影响。结果与 WR基底动脉相比, SHR基底动脉的KCl和5-HT量效曲线明显右移,再复K+所致血管舒张作用减弱,提示高血压病变可明显降低脑血管钠泵活性及 KCl 和5-HT 的收缩反应;哇巴因(Ouabain, OUA)浓度依赖性收缩SHR脑血管,其量效关系曲线可经两点结合模型进行最佳拟合,Kd分别为:1.7×10-8 mol·L-1和1.6×10-5 mol·L-1,表明SHR脑基底动脉上存在高、低亲和力两种不同功能的钠泵。用5×10-7 mol·L-1和10-4 mol·L-1的OUA分别抑制高、低亲和力钠泵,仅能在 SHR而非WR脑血管明显左移KCl和5-HT量效曲线,表明OUA可明显增强KCl和5-HT对脑血管的收缩作用,且OUA增强5-HT收缩SHR 脑血管的作用呈明显的浓度依赖性(r =0.9393,P<0.05);在SHR脑血管,这两个浓度OUA对KCl量效曲线的左移幅度无明显差别,而对5-HT收缩量效曲线的左移幅度却有明显不同,提示仅高亲和力钠泵介导了SHR脑血管对KCl的收缩反应,而SHR脑血管对5-HT的收缩反应则由高、低亲和力两种钠泵共同参与。结论高血压病变可明显降低脑血管对血管收缩剂的反应性,其机制可能与高血压所致钠泵活性降低或钠泵对OUA敏感性增加有关。
    • 候爽; 孟雨; 崔晓莉; 于飞飞; 杨博; 田洪艳
    • 摘要: 目的 探讨蒺藜皂苷对热损伤小鼠睾丸钠泵表达的影响.方法 雄性小鼠30只,随机分为对照组、模型组和给药组,每组10只.建立睾丸热损伤模型,蒺藜皂苷灌胃(300 mg/kg,1t/d,8w).一半小鼠取附睾精子进行精子参数检测;一半小鼠心脏灌流固定,进行免疫组织化学染色检测睾丸组织钠泵的表达.结果 模型组与对照组比较,精子密度、活力、活率均显著下降,畸形率明显升高;生精细胞数目减少、排列紊乱;睾丸组织钠泵表达降低.给药组与模型组比较,精子密度、活力、活率升高,畸形率下降;生精细胞数目增多、排列紧密;睾丸组织钠泵表达升高.结论 蒺藜皂苷能够通过调节钠泵表达对小鼠热损伤生殖功能的保护作用.
    • 候爽1; 孟雨1; 崔晓莉1; 于飞飞1; 杨博1; 田洪艳2
    • 摘要: 目的探讨蒺藜皂苷对热损伤小鼠睾丸钠泵表达的影响。方法雄性小鼠30只,随机分为对照组、模型组和给药组,每组10只。建立睾丸热损伤模型,蒺藜皂苷灌胃(300 mg/kg,1 t/d,8 w)。一半小鼠取附睾精子进行精子参数检测;一半小鼠心脏灌流固定,进行免疫组织化学染色检测睾丸组织钠泵的表达。结果模型组与对照组比较,精子密度、活力、活率均显著下降,畸形率明显升高;生精细胞数目减少、排列紊乱;睾丸组织钠泵表达降低。给药组与模型组比较,精子密度、活力、活率升高,畸形率下降;生精细胞数目增多、排列紧密;睾丸组织钠泵表达升高。结论蒺藜皂苷能够通过调节钠泵表达对小鼠热损伤生殖功能的保护作用。
    • 张明超; 汪和贵; 柯永胜
    • 摘要: 钠泵不仅是细胞膜内外离子跨膜转运的重要通道,还参与细胞内多种信号转导通路的调节。研究表明,钠泵通过调节细胞内外钙离子浓度以及信号转导参与心肌缺血‐再灌注损伤的过程。该文就心肌缺血‐再灌注损伤中钠泵及其下游信号转导通路的研究进展作一介绍。
    • 冯东霞; 宋文琪; 王艳; 李爱华
    • 摘要: Objective To detect the serum Na pump α1 submit autoantibody in pediatric patients with acute glomerulonephritis and to e-valuate its effect on activity of Na pump. Methods A total of 195 pediatric patients with acute glomerulonephritis were enrolled in this study. A-mong them,33 cases had established follow - up during thecourse of illness and three months to six months after recovery of the disease. Results The serum level of Na pump α1 subunit antibody in patients with acute glomerulonephritis was significantly higher than that of control group( P﹤ 0. 05),the percentage of affection in girls were significantly higher than that of boys( P ﹤ 0. 05),and it mainlypresented in IgM class antibod-ies. Serum Na pump α1 IgM antibody was significantly decreased in 3 months after onset of the disease( P ﹤ 0. 05),but serum level of IgG anti-body was significantly increased than that of controls( P ﹤ 0. 05). Serum level of Na pump α1 IgG antibody was significantly higher than that of controls after 6 months( P ﹤ 0. 05)and serum level of IgM antibody was similar to that of controls. IgG Na pump α1 subunit antibody could sig-nificantly inhibit the activity of Na pump in HEK - 293A cells( P ﹤ 0. 05),but IgM antibody had no such effect( P ﹥ 0. 05). Conclusion Na pump α1 subunit autoantibodies can be detected in pediatric patients with acute glomerulonephritis. IgG Na pump α1 subunit autoantibody can in-hibit the activity of Na pump,and it may play animportant role in long - term renal impairment in patients with acute glomerulonephritis.%目的:检测急性肾小管肾炎患者血清抗钠泵α1亚基抗体的水平,并评价其对钠泵功能的影响。方法急性肾小球肾炎患儿195例,并对其中33例建立随访,分别于疾病期间及疾病痊愈后3个月和6个月检测血清抗钠泵α1亚基抗体的水平。同时使用受试者血清作用 HEK -293A 细胞膜蛋白后检测钠泵活性。结果急性肾小球肾炎患者血清抗钠泵α1亚基抗体水平较对照组显著升高( P ﹤0.05),女性显著高于男性( P ﹤0.05),且主要表现为 IgM 类抗体升高。3个月后血清 IgM 抗钠泵α1亚基抗体水平较患病时显著下降( P ﹤0.05),但 IgG 抗钠泵α1亚基抗体水平较患病时显著升高( P ﹤0.05)。6个月后 IgM 抗体平均降至表面健康对照组水平,但 IgG 类抗体水平仍高于对照组( P ﹤0.05)。细胞实验结果表明,IgG 类抗钠泵α1亚基能显著抑制人肾脏细胞系 HEK -293A 细胞的钠泵活性( P ﹤0.05),但 IgM 类抗体无上述作用( P ﹥0.05)。结论急性肾小球肾炎患者血清中可存在钠泵α1亚基自身抗体。IgG 类钠泵α1亚基自身抗体能抑制钠泵的活性,这可能是导致急性肾小球肾炎慢性长期肾损伤的机制之一。
    • 张明娟; 张美程; 朱参战; 张超英; 段宗明
    • 摘要: Objective To prepare polyclonal antibodies against sodium pump alpha 2 subunit M1-M2 extramembrane fragment (NKAα2 EM1) for studying the pathogenesis of hypertension. Methods According to the GenBank data, the amino acid sequence of NKAα2 EM1 was obtained and the target peptide (LAAMEDEPSNDN) was synthesized using a peptide synthesizer with Fmoc method and purified with high-performance liquid chromatography. The synthesized peptide was then coupled to KLH for immunizing New Zealand white rabbits for 4 times to obtain the antiserum. The IgG antibodies against the synthetic peptide, after affinity purification with Protein A, were used for detecting NKAα2 EM1 expression in rat aortic vascular smooth muscle cells by enzyme- linked immunosorbent assay and immunocytochemistry (ICC). Results The synthesized peptide fragment , which consisted of 13 amino acid residues including one derivatized cysteine residue in the N-terminal (LAAMEDEPSNDN-C), had a theoretical relative molecular mass of 1408.48 D with a measured relative molecular mass of 1407.90 D and a purity exceeding 85.5%. The titer of the antiserum was more than 1:512 000, and the purified IgG antibody concentration was 0.965 mg/ml after purification with Protein A. At a 1:1000 dilution (final concentration of 1μg/ml), the titer of the purified IgG antibody was more than 1: 256 000. The purified IgG antibody could be used at 1:100 to 1:200 dilutions for for immunocytological examination of formalin-fixed cells. Conclusion The anti-NKAα2 EM1 polyclonal antibodies obtained can be used in ELISA and immunocytochemistry for detecting the sodium pump alpha 2 subunit in formalin-fixed tissue or cells to facilitate investigation of the relationship between sodium pump and hypertension.%目的:采用多肽制备技术和免疫学技术制备抗钠泵α2亚单位截断性片段及其抗体,为检测和研究钠泵α2亚单位的组织学分布及其功能研究提供实验基础。方法根据NCBI-Genebank获得大鼠钠泵α2亚单位M1~M2膜外区截断性片段目的多肽(LAAMEDEPSNDN),采用9-氟甲氧羰基(Fmoc)固相合成法合成目的多肽,并采用碳化二亚胺法制备出多肽与孔戚血蓝素复合物,免疫新西兰大白兔,免疫4次后获得抗血清,测定其效价。随后采用protein A纯化IgG抗体,并将其用于大鼠主动脉血管平滑肌细胞钠泵α2亚单的组织学检测。结果(1)人工合成的大鼠钠泵α2亚单位截断性片段长13氨基酸残基(LAAMEDEPSNDN-C),理论相对分子质量:1408.48,质谱实测相对分子质量:1407.90;高效色谱分析纯度HPLC纯度>85.5%;(2)ELISA法检测免疫后兔子的抗血清效价均大于1∶512000,蛋白A亲和纯化获得亲和纯化抗体浓度为0.965 mg/ml,按照1∶1000稀释(终浓度1μg/ml),ELISA检测抗体效价为1∶256000;(3)该抗体按照1∶100~1∶200稀释可用于免疫细胞学检测。结论成功制备高效价抗钠泵α2亚单位截断性片段抗体可用于钠泵α2亚单位的ELISA和免疫细胞化学实验,为钠泵α2亚单位的组织细胞学检测和功能研究提供新的实验基础。
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