摘要：
[目的]建立高效液相色谱方法快速测定马铃薯茎叶、块茎及其土壤中霜脲氰残留量的方法.[方法]样品经乙腈提取,NH2固相萃取小柱净化后,以乙腈-水为流动相,使用C18色谱柱,通过Waters 2695高效液相色谱-紫外检测器,在254nm检测波长下对霜脲氰进行分析检测,面积外标法定量.[结果]在0.1、0.5、2.0 mg/kg 3个不同添加浓度水平下,马铃薯茎叶中平均回收率为78.0％～104.4％,相对标准偏差为2.7％ ～3.4％;马铃薯块茎中平均回收率为82.9％～94.7％,相对标准偏差为1.1％ ～2.3％;土壤中平均回收率为81.6％～91.8％,相对标准偏差为3.3％ ～5.4％.霜脲氰在浓度为0.05～2.00 mg/L时线性关系良好(r=0.999 8),最小检出量为0.06 ng,在马铃薯茎叶、块茎和土壤中的最低检测浓度均为10 μg/kg.[结论]该方法简便、快捷、成本低、精密度好、准确率高,适用于马铃薯及其土壤中霜脲氰的大批量分析检测,是一种较为实用的检测方法.%[Objective] A rapid determination method was established for the residue of cymoxanil in potato stem leaf,tuber and soil by HPLC.[Method] The samples were extracted with acetonitrile and purified by NH2 solid-phase extraction column.Analysis and determination were conducted by Waters 2695 HPLC with C18 column and UV detector at 254 nm,and CH3CN-H2O was utilized as the mobile phase.The residues were quantified by area of external standard method.[Result] When the concentration levels were added at different levels of 0.1,0.5 and 2.0 mg/kg,the average recoveries of residues were 78.0％-104.4％ with RSD of 2.7％-3.4％ in potato stem leaves,and 82.9％-94.7％ with RSD of 1.1％-2.3％ in potato tubers,and 81.6％-91.8％ with RSD of 3.3％-5.4％ in soil.The linear relationship was obtained in the concentration that range from 0.05 to 2.00 mg/L for cymoxanil (r =0.999 8).The minimum detectable amount of cymoxanil was 0.06 ng.The minimum detectable concentrations of cymoxanil was 10 μg/kg in potato stem leaf,tuber and soil.[Conclusion]This method has advantages of simple,rapid,low cost,good precision and high accuracy,which is a practical detection method for the large batch analysis of cymoxanil in potato and soil.
