电泳,毛细管

摘要： 目的 评估毛细管电泳在良恶性单克隆球蛋白血症(MG)的诊断及鉴别诊断中的应用价值.方法 回顾分析青岛大学附属医院2016年1月至2020年6月门诊及住院初诊患者2 445例的毛细管电泳检测结果,通过青岛大学附属医院临床大数据库收集患者的临床诊断等信息.多组间单克隆球蛋白含量差异比较采用Kruskal-Wallis等级秩和检验,通过受试者工作特征(ROC)曲线分析各型单克隆球蛋白的诊断敏感度及特异度,采用约登指数计算截断值.结果 2445例初诊患者中,单克隆球蛋白阳性1 183例,其中诊断为恶性MG 944例,未定性的单克隆γ球蛋白血症(MGUS)174例,具有肾脏意义的单克隆球蛋白血症(MGRS)65例.单克隆球蛋白分型比例由高到低为IgG-κ型、IgG-λ型、IgA-λ型、IgA-K型、游离λ轻链型、游离K轻链型、IgM-κ型、双克隆型及IgM-λ型.恶性MG组的IgG、IgA、IgM及游离轻链型(FLC)型单克隆球蛋白含量均高于MGUS组,差异有统计学意义(P＜0.01).恶性MG组与MGRS组比较,IgG及IgA型单克隆球蛋白含量差异有统计学意义(P＜0.01).ROC曲线分析IgG、IgA、IgM及FLC型单克隆球蛋白对恶性MG均有较好的诊断效能(P＜0.01),ROC曲线下面积分别为0.947(95％CI 0.926～0.968)、0.930(95％CI 0.895～0.966)、0.844(95％CI 0.722～0.967)和0.865(95％CI0.781～0.950).IgG型单克隆球蛋白截断值为14.24 g/L,诊断敏感度及特异度为88.5％和90.1％;IgA型单克隆球蛋白截断值为8.88 g/L,敏感度及特异度为87.9％和81.4％;IgM型单克隆球蛋白截断值为26.93 g/L,敏感度及特异度为64.4％和90.9％;FLC型单克隆球蛋白截断值为7.08g/L,诊断敏感度85.9％,特异度77.8％.结论 毛细管电泳免疫分型技术可用于多发性骨髓瘤、非霍奇金淋巴瘤等恶性MG的诊断,也可用于MGUS、MGRS疾病的筛查及追踪;血清单克隆球蛋白水平可有效鉴别良恶性MG.

摘要： Objective To adopt the capillary electrophoresis(HPCE) for detecting the contents of emodin,chrysophanol and rhein in the extract of rheum officinale.Methods The detection was executed with a fused-silica capillary column(67.4 cm× 75.0 μm,effective length 51.0cm) as the separation column.The buffer solution consisted of 60 mmol/L Na2B4O7,40 mmol/L Na2CO3 and 40 mmol β-cyclodextrin(pH 8.9).The detection wavelength was 254 nm.Results RSD of precision in emodin,chrysophanol and rhein was 1.79％,4.46％ and 2.30％.respectively The linear range of 3 components was 1.1-19.0 mg/mL;the average recovery rates of emodin,chrysophanol and rhein were 100.14 ％,99.65 ％ and 98.44 ％ respectively,RSD was 2.43 ％,2.54 ％ and 2.02％ respectively(n=3).Conclusion The method can be used in the content determination of rheum officinale extract.%目的 采用毛细管区带电泳测定大黄提取物中大黄素、大黄酚和大黄酸的含量.方法 以熔融石英毛细管柱(67.4cm×75.0 μm,有效长度51.0 cm)为分离柱,60 mmol/L Na2B4O7,40 mmol/L Na2CO3,40 mmol/L β-环糊精(pH 8.9)为缓冲溶液,检测波长254 nm.结果 大黄提取物含量测定中大黄素、大黄酚和大黄酸精密度的相对标准偏差(RSD)分别为1.79％、4.46％和2.30％;3组分共同线性范围为1.1～19.0 mg/mL;大黄素、大黄酚和大黄酸的平均回收率分别为100.14％、99.65％和98.44％,RSD分别为2.43％、2.54％和2.02％(n=3).结论 该方法可用于大黄提取物的含量检测.

摘要： Objective To establish and evaluate the application of modified capillary immunotyping for cryoglobulin qualification .Methods Referred to literature and benchwork experience , a modified capillary immunotyping technique was set up for cryoglobulin identification . Seventy-eight cryoglobulin positive specimens were collected by a standard method in Peking Union Medical College Hospital from November 2016 to July 2017.Thirty-nine samples were identified the type of the cryoglobulin simultaneously by modified capillary immunotyping ( CI ) and immunofixation electrophoresis ( IFE ) .Results Using the modified capillary immunotyping method , the types of cryoglobulin in seventy-eight specimens were identified.The number of cases decreased in the order of Ⅲ, Ⅱ and Ⅰ type of cryoglobulin .The clinical characteristics coincidence with previous reports .The modified CI method had a dramatic advantage in the speed, clarity, and accuracy of results compared with IFE .The ratio of reportable cases between these two methods was 1:0.87.Conclusion The modified capillary immunotyping was an accurate and easy method for cryoglobulin qualification , and feasible for clinical application .%目的 改良毛细管免疫分型技术用于冷球蛋白鉴定的方法建立与临床应用.方法 参考文献及工作经验,建立改良毛细管免疫分型技术用于冷球蛋白鉴定.严格按照冷球蛋白采样要求,收集2016年11月至2017年7月在北京协和医院检测冷球蛋白阳性的标本78份,其中39份分别采用改良的毛细管免疫分型和免疫固定电泳方法进行分型鉴定.结果 采用改良毛细管免疫分型技术,78例冷球蛋白阳性标本中鉴定出Ⅲ型冷球蛋白最多,其次是Ⅱ型、Ⅰ型,不同类型冷球蛋白血症患者临床特点与既往报道相似.在检测速度、结果判读的清晰度和准确度上,改良毛细管免疫分型技术明显优于免疫固定电泳方法,两者可报告比例为1:0.87.结论 毛细管免疫分型技术是一种准确而简便易行的冷球蛋白鉴定方法,适于临床实验室使用.

摘要： 目的 以国际临床化学与检验医学联合会(IFCC)糖化血红蛋白参考方法为参比方法,对4个离子交换高效液相色谱法检测系统和1个毛细管电泳检测系统进行方法学评价研究.方法 制备40个浓度水平的溶血液样本,分别用IFCC参考方法和待评价的5个糖化血红蛋白检测系统进行测定,按照美国临床实验室标准化协会(CLSI)EP9-A3文件的程序进行正确度验证.使用全血样本,对5种系统的精密度、线性和分析干扰进行评价.结果 IFCC参考方法测定40个溶血液样本的平均变异系数(CV)为1.4％(范围0.2％～2.5％).经统计,5个系统测定结果未检出离群值,回归方程的斜率范围为0.9902～1.0267,截距范围为-0.1526 mmol/mol～+0.1512 mmol/mol,决定系数的范围为0.9962～0.9971,两个医学决定水平处估计值的偏倚均小于0.3％HbA1c.各系统实验室内CV均小于2％(NGSP单位).线性评价中所有系统测定结果与理论值的百分偏差均小于5％.高浓度葡萄糖对糖化血红蛋白检测存在一定的分析干扰.结论 5个糖化血红蛋白检测系统测定结果与IFCC参考方法具有较好的可比性,精密度和线性评价的结果符合相关标准的要求.%Objective To perform a methodological evaluation study of 4 HPLC based systems and a capillary electrophoresis based system , with the International Federation of Clinical Chemistry and Laboratory Medicine ( IFCC) glycated hemoglobin reference method as a comparative method .Methods 40 hemolysis samples of variety concentrations were prepared .The samples were measured by IFCC reference method and 5 glycated hemoglobin testing systems , respectively and trueness verification was performed according to the Clinical &Laboratory Standards Institute (CLSI) guideline EP9-A3.Whole blood samples were used to test the systems'precision, linearity and analytical interferences .Results The average CV of IFCC reference method results of 40 hemolysis samples was 1.4％( range from 0.2％to 2.5％).No outlier was found in the results of the 5 testing system.The slopes ranged from 0.9902 to 1.0267, and intercepts from -0.1526 mmol/mol to +0.1512 mmol/mol, squared correlation coefficient from 0.9962-0.9971, biases at two medical decision level were less than 0.3％HbA1c.Within-laboratory precisions were less than 2％ (NGSP unit).Bias between all test results and predicted results were less than 5％.High concentration of glucose showed certain interference to glycated Hemoglobin tests but had little influence in clinical practice.Conclusions The results of the 5 testing system are comparable to the IFCC reference method , the results of precision and linearity evaluation meet the requirements of related guidelines .

摘要： Objective To develop a platform method using imaged capillary isoelectric focusing (iCIEF) for measuring charge heterogeneity of monoclonal antibody (mAb) and validate its suitability with different isoforms of mAbs (IgG1,IgG2,IgG4).Methods The volumes of carrier ampholyte and cathode stabilizer,focusing time,and urea concentration were optimized in iCIEF method development.The specificity,precision,linearity,accuracy,and robustness of this method were validated using 3 isoforms of mAbs (IgG1,IgG2,IgG4).Results Under following conditions:70μl 3 mol/L urea-0.5 ％ methyl cellulose,4 μl broad-range carrier ampholyte (pH3-10),2 μl cathode stabilizer (500 mmol/L Arg),2μl each isoelectric point 6.14 and 9.99 Markers,0.2 mg/ml mAb (sample) was prepared.The detection parameters were determined to be pre-focusing 1 min at 1 500 V and focusing 8 min at 3 000 V.The specificity of the platform method was good,and formulation buffer had no interference with detection.Repeated testing of 6 parallel samples and testing 12 samples by different persons at different time both showed that,the relative standard deviations of all compositions met requirements.The linear coefficients of determination (R2) were ≥0.99 for main and acidic compositions in mAb and ≥0.98 for basic composition,when the final concentrations of mAbs (samples) were 0.1-0.3 mg/ml.The accuracies of the platform method for detecting different composition contents in mAbs were 92％-105 ％.The robustness results of design of experiment showed that carrier ampholyte volume and capillary lot had significant impacts on this platform method.Conclusions The iCIEF platform method for analysis of charge heterogeneity of different isoforms of mAbs is developed,which has high separation resolution and good precision,accuracy,and robustness.This method can provide a more effective tool for charge heterogeneity characterization and quality control of mAbs.%目的 采用成像毛细管等电聚焦电泳技术(imaged capillary isoelectric focusing,iCIEF)建立分析单克隆抗体(单抗)电荷异质性的平台方法,并用不同亚型单抗(IgG1、IgG2、IgG4)确认该平台方法的适用性.方法 优化该平台方法的部分参数,包括两性电解质和阴极稳定剂体积、聚焦时间和尿素浓度.采用3种亚型单抗(IgG1、IgG2、IgG4)对该平台方法的专属性、精密度、线性、准确度和耐用性进行验证.结果 对样品(单抗)的处理条件为:3 mol/L尿素-0.5％甲基纤维素溶液70μl、两性电解质(pH3～10)4 μl、阴极稳定剂(500 mmol/L精氨酸)2 μl、等电点6.14和9.99 Marker各2μl,最终完成0.2 mg/ml单抗(样品)的制备.检测参数:预聚焦1 500V、1 min,聚焦3 000V、8 min.该平台方法的专属性良好,制剂缓冲液对检测无干扰.重复检测6份平行样品以及不同分析员于不同时间检测12份样品各成分含量的相对标准偏差均符合规定的要求.单抗(样品)终浓度为0.1～0.3 mg/ml时,主要和酸性成分的线性决定系数(R2)≥0.99,碱性成分的线性R2≥0.98.该平台方法检测样品各成分的准确度为92％～105％.耐用性实验设计结果表明,两性电解质(pH3～10)体积和毛细管批次对该平台方法有显著影响.结论 建立的iCIEF平台方法分离度较高,精密度、准确度和耐用性良好,为单抗制品的电荷异质性表征和质量控制提供了更有效的工具.

摘要： 目的 建立改进的四引物扩增受阻突变体系PCR(T-ARMS-PCR)检测方法,实现单管一次性检测叶酸代谢过程相关的4个单核苷酸多态性(SNP)位点:rs1801133,rs1801131,rs1805087和rs1801394.方法 方法学建立.于2017年1至4月在河北省儿童医院检验科收集150名体检儿童抗凝全血标本以待验证.利用T-ARMS-PCR原理及多重嵌合引物策略设计rs1801133、rs1801131、rs1805087及rs1801394的特异性内外嵌合引物.通过优化引物浓度和反应条件,经单管PCR反应,结合QIAxcel毛细管电泳分析扩增产物长度,判读150份标本的SNP基因型,同时所有标本经测序法进一步验证.利用SPSS 17.0统计软件卡方检验对150份标本的4个SNP位点分别进行哈迪-温伯格遗传平衡(HWE)检测.结果 改进的T-ARMS-PCR结合毛细管电泳分析的方法可在3 h内一次性识别本研究中叶酸代谢过程相关的4个SNP位点的8个不同等位基因,可对150份全血标本的SNP位点准确分型,其分型结果与测序法完全相符.4个SNP位点基因型的分布均符合HWE平衡定律(χ2rs1801133=0.69,Prs1801133=0.40; χ2rs1801131=0.21, Prs1801131=0.64; χ2rs1805087=3.32, Prs1805087=0.07;χ2rs1801394=1.91,Prs1801394=0.17).结论 本研究建立的改进的T-ARMS-PCR结合毛细管电泳分析方法,可准确地同时检测叶酸代谢过程相关的4个SNP位点,为指导人群特异性地补充叶酸提供了一种可能的技术手段.%Objective To develop a tetra-primer amplification refractory mutation system PCR(T-ARMS-PCR)assay for detecting four single nucleotide polymorphisms(SNPs): rs1801133,rs1801131, rs1805087 and rs1801394 associated with folate metabolism in a single reaction tube.Methods Methodology was developed.Then, it applied the established method to analyze 150 physical examination children′s anticoagulant blood samples admitted to the Department of Clinical Laboratory, Children′s Hospital of Hebei Province from January 2017 to April 2017.Four sets of chimeric primers consisting of a universal Tag sequence and targeting rs 1801133,rs1801131,rs1805087 and rs1801394 fused to the specific sequence were designed according to the T-ARMS-PCR principle and the multiplex chimeric primers strategy.A single tube PCR was then conducted after optimizing the primer concentrations and the reaction conditions.The amplified products were analyzed by QIAxcel capillary electrophoresis, and the corresponding SNP genotypes of 150 samples were identified.Furthermore,all samples were verified by direct sequencing.And the Hardy-Weinberg Equilibrium(HWE)testing of four SNPs from 150 samples were conducted by SPSS17.0 Chi-square test.Results The improved T-ARMS-PCR combined with capillary electrophoresis can accurately verify eight different alleles of the four SNPs associated with folate metabolism at one time in 3 hours.Four SNPs of 150 whole blood samples were accurately classified and the results were completely consistent with direct sequencing.All the genotype frequencies of these four SNPs were in HWE (χ2rs1801133=0.69, Prs1801133=0.40; χ2rs1801131=0.21, Prs1801131=0.64; χ2rs1805087=3.32, Prs1805087=0.07;χ2rs1801394=1.91, Prs1801394=0.17).Conclusions The proposed improved T-ARMS-PCR combined with capillary electrophoresis in this study can accurately verify four SNPs associated with folate metabolism in a single reaction tube.This method might be a valuable tool to specifically guide the folate supplement in general population.

摘要： Objective To detect twenty types/subtypes of respiratory viruses with the GeXP (genomelab genetic analysis system) based mRT-PCR (multiplex reverse transcription-polymerase chain reaction) assay,and discuss its value in pediatric respiratory viral infection.Methods A retrospective study was conducted on a total of 1 305 inpatient children (0-6 years old) admitted to the Department of Respiratory in Children's Hospital of Hebei Province from May 2014 to November 2014.The sputum samples were collected and the viral DNA or RNA was extracted.After examining the specificity and sensitivity,the GeXP-based mRT-PCR assay was performed to test 20 types/subtypes of respiratory viruses.The obtained results were evaluated in comparison with those of fluorescence real-time PCR and direct sequencing.Chisquare test was conducted by SPSS16.0.Results Among 1 305 hospitalized children,the positive rate of respiratory virus was 58.31％ (761/1 305).The positive rate of respiratory syncytial virus (RSV,17.32％,226/1 305) was the highest followed by the parainfluenza virus 3 (PIV-3,16.02％,209/ 1 305),human rhinovirus (HRV,11.95％,156/1 305),adenovirus (ADV,5.06％,66/1 305),human bocavirus (HBoV,3.14％,41/1 305) and the parainfluenza virus 1 (PIV-1,2.07％,27/1 305).Multiple virus infection was found in 92 children (7.05％).The highest positive rate of respiratory virus was observed in 1-2 years old children (83.28％,249/299);the lowest rate was found in 5-6 years old children (37.68％,26/69).Conclusions The proposed GeXP-based mRT-PCR assay possessed the advantage of high throughput,sensitivity,specificity and rapidity in the detection of twenty types/subtypes of respiratory viruses.It provided a thorough investigation of viral pathogens involved in acute respiratory infection thus could satisfy the clinic requirement and provide epidemic data on the disease prevention.%目的 利用GeXP多重基因表达遗传分析系统联合多重逆转录-聚合酶链反应(mRT-PCR)方法同时检测20种呼吸道病毒并探讨其在儿童呼吸系统病毒感染病原体检测中的价值.方法 本文采用回顾性研究,分析1 305例2014年3月至11月在河北省儿童医院呼吸科呼吸系统病毒感染住院患儿,年龄0～6岁,采集其抽吸痰液,提取病毒DNA/RNA,利用mRT-PCR扩增技术,通过GeXP分析平台进行毛细管电泳,同时检测20种呼吸道病毒,检测该方法的特异性和灵敏度,并使用荧光实时PCR和直接测序法评价其准确度,利用SPSS16.0统计软件卡方检验进行统计分析.结果 在1 305例住院患儿中,呼吸系统病毒感染阳性检出率为58.31％ (761/1 305).其中,呼吸道合胞病毒阳性率最高,为17.32％ (226/1 305);其次,副流感病毒3型阳性率为16.02％ (209/1 305);鼻病毒、腺病毒、博卡病毒和副流感病毒1型的阳性率分别为11.95％(156/1 305)、5.06％ (66/1 305)、3.14％ (41/1 305)和2.07％ (27/1 305).此外,同时感染2种及2种以上病毒的混合感染患儿92例,阳性率7.05％.1～2岁患儿病毒检出率最高,为83.28％ (249/299);5～6岁患儿病毒检出率最低,为37.68％ (26/69).结论 利用GeXP分析平台联合多重RT-PCR技术同时检测20种呼吸道病毒,具有高通量、高灵敏度、特异性强且检验速度快等优点,其高效性能够满足临床对呼吸道病毒检测的要求,并为儿童急性呼吸道感染的防治提供资料.

摘要： 目的 探讨中间型地中海贫血的临床诊治思路.方法 选择2012年3月15日及2013年4月29日在中山大学孙逸仙纪念医院就诊并确诊为中间型地中海贫血的2例患儿为研究对象,结合相关文献复习其临床表现、治疗及转归.本研究遵循的程序符合中山大学孙逸仙纪念医院人体试验委员会制定的伦理学标准,得到该委员会批准,并与受试对象监护人签署临床研究知情同意书.对2例患儿行入院检查,并对其珠蛋白基因变异类型进行检测.结果 患儿1为3岁,女性,临床表现为面色苍白2+年,患儿腹部出现包块并逐渐增大1+年.采用PCR结合DNA序列测定等方法行罕见型突变检测,确诊为β地中海贫血基因(β珠蛋白CD41/42基因缺失突变型)杂合子合并a地中海贫血基因(aaaanti3.7基因重排)杂合子.患儿2为5岁,男性,临床表现为面色苍白4年.通过毛细管电泳及基因突变检测,确诊为HbH-CS病.结论 中间型地中海贫血的诊断需考虑多种基因变异因素对临床表现的影响,毛细管电泳与基因序列分析在诊断中间型地贫中结合使用具有重要的应用价值.

摘要： 目的：探讨强化血液灌流（HP）治疗急性百草枯（PQ）中毒患者的血清PQ清除率及对患者预后的影响。方法选取2010年3月—2013年10月连云港市第一人民医院收治的PQ中毒患者65例，其中14例拒绝接受HP治疗，作为对照组；其余51例按照就诊时间分为常规HP组（25例）和强化HP组（26例）。3组患者均给予综合治疗，常规HP组患者在洗胃后即给予1次HP治疗，强化HP组进行3次HP治疗，间隔时间为6 h。建立PQ标准曲线，3组均在HP前（T1）及HP后即刻（T2）、8 h（T3）、16 h（T4）抽取静脉血，利用曲线得出PQ水平，并计算PQ清除率。动态监测3组患者肌酐（Cr）、尿素氮（BUN）、白细胞计数（WBC）、天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ ALT）及动脉血氧分压（ PaO2）。结果3组患者性别、年龄、服毒量、服毒至入院时间、入院时PQ水平、入院时Cr、BUN峰值、ALT峰值、AST峰值、WBC峰值比较，差异均无统计学意义（ P＞0.05）；3组急性生理与慢性健康状况评分系统Ⅱ（APACHEⅡ）评分、Cr峰值、PaO2最低值比较，差异均有统计学意义（P＜0.05）。PQ标准曲线在0.25˜16.00 mg/L有良好线性关系：y=0.0316x＋0.0112。3组患者不同时间点血清PQ水平比较，差异有统计学意义（ P＜0.05）。组间比较结果显示：强化组在T2、T3和T4时血清PQ水平均低于对照组，常规组在T2时血清PQ水平低于对照组，差异均有统计学意义（ P＜0.05）。组内比较结果显示：强化组、常规组在T2、T3、T4时间点血清PQ水平均低于T1，差异有统计学意义（P＜0.05）；对照组在T3、T4时间点血清PQ水平低于T1，差异有统计学意义（P＜0.05）。强化HP组3次PQ清除率分别为62%（16/26）、42%（11/26）、31%（8/26），PQ清除率比较，差异有统计学意义（χ2=5.079，P＜0.05）。常规HP组PQ清除率为56%（14/25）。常规HP组和强化HP组首次PQ清除率比较，差异无统计学意义（χ2=0.161，P ＞0.05）。Kaplan -Meier 生存曲线显示强化 HP 组生存率为46.2%（12/26）、常规HP组为36.0%（9/25）、对照组为14.3%（2/14）。3组生存率比较，差异有统计学意义（χ2=7.206，P=0.027），其中强化HP组生存率高于对照组（χ2=7.375，P=0.007），强化HP组生存率和常规HP组比较，差异无统计学意义（χ2=1.337，P=0.248），常规HP组生存率和对照组比较，差异无统计学意义（χ2=2.568， P=0.109）。结论强化HP有可能提高患者生存率，改善患者生存状态，单次HP效果劣于强化HP治疗；急性PQ中毒患者应在综合治疗的基础上尽早、多次给予HP治疗，HP治疗时间间隔不宜大于6 h。%Objective To explore the clearance rate of strengthened hemoperfusion( sHP) for acute paraquat( PQ) poisoning and its effects on patientsˊprognosis. Methods 51 PQ poisoning patients admitted to the First Peopleˊs Hospital of Lianyungang from March 2010 to October 2013 were divided, according to visiting time, into groups convention HP ( CHP group,n=25),strengthened HP(sHP group,n =26),another 14 patients who refused HP treatment enrolled in control group. All patients were given comprehensive treatment. CHP group given 1 HP treatment and sHP group given 3 ( the intervals was 6 h) after gastrolavage. A PQ standard curve was established. Venous blood was drawn and PQ concentration gained to calculate PQ clearance rates before HP(T1),instantly after HP(T2),at 8 h after HP(T3),at 16 h after HP(T4)in 3 groups. Creatinine( Cr ), blood urea nitrogen ( BUN ), white blood cell ( WBC ), aspartate aminotransferase ( AsT ), alanine aminotransferase( ALT) and were monitored dynamically. Results There was no significant difference in gender,age, poison amount,time from taking poison to hospital admission,PQ concentration at admission,Cr at admission,BUN peak, ALT peak,AsT peak,WBC peak among 3 groups(P>0. 05). There was a good linear correlation at 0. 25-16. 00 mg/L in PQ standard curve(y=0. 031 6x+0. 011 2). There was difference in serum PQ concentration among 3 groups at different time points(P0. 05). Kaplan-Meier survival curve showed that the survival rate was 46. 2% in sHP group(12/26),36. 0% in CHP group(9/25),14. 3% in control group(2/14);the difference was significant(χ2 =7. 206,P=0. 027). The survival rate was higher in sHP group than in control group(χ2 =7. 375,P=0. 007),there was no difference between groups sHP,CHP(χ2 =1. 337,P=0. 248)or between groups CHP,control(χ2 =2. 568,P=0. 109). Conclusion sHP may increase patientsˊ survival rate and improve their survival state. single HP is inferior to sHP in effectiveness. Patients with acute PQ should be given early,repeated HP treatment based on comprehensive treatment. The intervals between HP treatments should be no more than 6 h.

摘要： 目的：探讨醋酸纤维素电泳和毛细管电泳在血红蛋白（Hb）E病筛查中的价值差异。方法采用pH8．6醋酸纤维素电泳及SEBIA capillarys2全自动毛细管电泳仪对基因诊断确诊为 HbE阳性的17例地中海贫血患者的血红蛋白组分进行检测。结果17例 HbE阳性患者醋酸纤维素电泳 HbA2＋ HbE吸光度最高值为86．43，最低值为16．95，相应的毛细管电泳HbE＋ HbA2吸光度最高值为91．3（6．8＋84．5），最低值为19．8（3．2＋16．6）；2种方法对 HbE病的诊断结果高度一致，与基因诊断的相符率均为100％。结论醋酸纤维素电泳和毛细管电泳在HbE的筛查中结果一致。%Objective To study the value difference of cellulose acetate electrophoresis and capillary electrophoresis in screening for hemoglobin E(HbE) disease .Methods Cellulose acetate electrophoresis and SEBIA capillarys2 automated capillary electropho-resis instrument were employed to detect the hemoglobin components of 17 patients with thalassemia and positive HbE which were confirmed by genetic diagnosis .Results The highest and the lowest absorbance values of HbA2+ HbE of 17 patients with positive HbE according to cellulose acetate electrophoresis were 86 .43 and 16 .95 ,respectively ,while the corresponding absorbance values were 91 .3(6 .8+84 .5) and 19 .8(3 .2+16 .6) according to capillary electrophoresis ,respectively .The diagnostic results of the two methods for HbE were highly consistent and they matched well with genetically diagnostic results with the match rate of 100% . Conclusion Cellulose acetate electrophoresis and capillary electrophoresis have consistent screening results for HbE .

摘要： 能够全自动地实现从前处理至电泳的全部的工序。提供前处理电泳用一体型盒，具有与构成前处理的各个工序对应的一个或者多个区域构造，各区域构造具有：(1)第一区域，其具有反应槽、试剂槽、对各槽之间进行连接的多个流路、以及配置于上述流路的多个调节阀，并且具有对位于前级的区域构造的反应槽与位于后级的区域构造的反应槽进行连接的流路；(2)第二区域，其具有泳动溶液槽、阴极缓冲液槽以及清洗液槽，并且具有对位于前级的上述区域构造的载体槽与上述泳动溶液槽进行连接的流路及其调节阀。

摘要： 本发明属于医药分析及检测领域，具体涉及一种用于毛细管电泳的微流控芯片。为了解决现有硅毛细管微流控芯片成本较高，灵敏度较低的问题，本发明提供一种用于毛细管电泳的离心微流控芯片。所述芯片包括载片和芯片盖；所述载片上设置有毛细管电泳通道，毛细管电泳通道沿芯片径向分布；毛细管电泳通道的旁边设置有冷却液通道；毛细管电泳通道的起始端连通样品池和缓冲液池，毛细管电泳通道的终端连通废液池；毛细管电泳通道的起始端设置有阴极插孔，终端设置有阳极插孔；毛细管电泳通道的终端设置有重力控制阀；所述冷却液通道的两端设置有冷却液连接口。该离心微流控芯片成本低、灵敏度高、处理通量高。

摘要： 本发明涉及一种通过糖化血红蛋白的毛细管电泳进行分析的方法，所述糖化血红蛋白包含于生物试样中且包含至少一条珠蛋白链，该珠蛋白链包含结合至N-端位置处的氨基酸的葡萄糖残基，其中所述方法包括使用包含至少一种化合物的缓冲液组合物，该化合物能够专一性复合一种或多种糖化血红蛋白的葡萄糖残基并且在碱性pH下能够向所述血红蛋白传递多个负电荷。举例而言，所述化合物可为3，4-或3，5-二羧基苯基硼酸、优选为3，5-二羧基苯基硼酸。所述方法尤其可用于分离及测定生物试样中存在的HbA1c血红蛋白，该生物试样任选包含其它血红蛋白、具体而言其它次要部分。本发明还涉及用于该分析中的缓冲液组合物、以及用于通过毛细管电泳进行的糖化血红蛋白的分析及测定的试剂盒。

摘要： 通过毛细管电泳分离蛋白质的方法和毛细管电泳用的缓冲组合物本发明涉及一种碱性pH、自由溶液毛细管电泳分析含蛋白质组分的生物样品的方法。所述方法其特征在于它包括至少一个步骤，其中将所述样品加入到含有分析缓冲液的毛细管中，所述分析缓冲液还含有至少一种添加剂，所述添加剂能够与一种或多种蛋白质组分疏水相互作用并且能够使所述蛋白质组分具有1个或多个负电荷并且能够改进其电泳迁移率。本发明还涉及用于进行这种方法的分析缓冲液组合物。

摘要： 本发明涉及碱性pH条件下分析含血红蛋白样品的自由溶液毛细管电泳方法，其中该样品流过含有分析缓冲剂的毛细管，包括至少一个样品被引入含有分析缓冲溶液的毛细管的步骤，其特征在于缓冲剂是两性离子型并且它与至少一种流动抑制剂联合。本发明还涉及与至少一种两性离子型缓冲剂联合的EC流动抑制剂的用途，和通过毛细管电泳分析血红蛋白的试剂盒。

摘要： 能够全自动地实现从前处理至电泳的全部的工序。提供前处理电泳用一体型盒，具有与构成前处理的各个工序对应的一个或者多个区域构造，各区域构造具有：(1)第一区域，其具有反应槽、试剂槽、对各槽之间进行连接的多个流路、以及配置于上述流路的多个调节阀，并且具有对位于前级的区域构造的反应槽与位于后级的区域构造的反应槽进行连接的流路；(2)第二区域，其具有泳动溶液槽、阴极缓冲液槽以及清洗液槽，并且具有对位于前级的上述区域构造的载体槽与上述泳动溶液槽进行连接的流路及其调节阀。

摘要： 本发明提供无需使用人的手便能够向超声波焊头的安装部插入毛细管的毛细管输送装置。本发明的一方案涉及一种毛细管输送装置，其具备输送毛细管(13)的第一管(17)、具有安装所述毛细管的安装部的超声波焊头(11)、使所述超声波焊头与所述第一管的第一端部(17a)相对移动的第一移动机构、以及向所述第一管的第二端部(17b)吹入气体的机构。

摘要： 本发明公开了毛细管电泳两步速差模式提高毛细管电泳峰容量的装置，包括负极贮液槽1，正极贮液槽2、样品槽4和毛细管5，还包括第一零极贮液槽3、第二零极贮液槽13，毛细管5贯穿第一零极贮液槽3和第二零极贮液槽13设置，毛细管5的一端与正极贮液槽2或样品槽4活动连接，毛细管5的另一端与负极贮液槽1或样品槽4活动连接，零极贮液槽内的毛细管设置有导电不传质的缝隙，利用本发明的装置在提高毛细管电泳峰容量中，降低了毛细管自身分离能力的要求，即便样品中部分组分不能达到基线分离，应用此方法最终也能实现基线分离，从而提高毛细管电泳整体峰容量。除此之外，此装置在增大峰容量的同时并不丢失低含量组分信号。

摘要： 本发明属于医药分析及检测领域，具体涉及一种用于毛细管电泳的微流控芯片。为了解决现有硅毛细管微流控芯片成本较高，灵敏度较低的问题，本发明提供一种用于毛细管电泳的离心微流控芯片。所述芯片包括载片和芯片盖；所述载片上设置有毛细管电泳通道，毛细管电泳通道沿芯片径向分布；毛细管电泳通道的旁边设置有冷却液通道；毛细管电泳通道的起始端连通样品池和缓冲液池，毛细管电泳通道的终端连通废液池；毛细管电泳通道的起始端设置有阴极插孔，终端设置有阳极插孔；毛细管电泳通道的终端设置有重力控制阀；所述冷却液通道的两端设置有冷却液连接口。该离心微流控芯片成本低、灵敏度高、处理通量高。

摘要： 本实用新型属于物质分离检测领域，涉及一种毛细管电泳装置和毛细管电泳质谱联用仪。所述毛细管电泳装置包括高压电源、缓冲溶液瓶、铂电极、毛细分离管Ⅰ、毛细分离管Ⅱ、四通阀、镀银喷针和输液泵，输液泵携带有样品瓶和洗剂瓶，铂电极的一端连接高压电源的正极而另一端插入缓冲溶液瓶中，四通阀的一个纳升级样品腔分别连接毛细分离管Ⅰ和毛细分离管Ⅱ，另一个纳升级样品腔分别连接输液泵和废液出口，所述毛细分离管Ⅰ的入口端插入缓冲溶液瓶中，毛细分离管Ⅱ的出口端通过微米级二通接口与镀银喷针相连，镀银喷针的表面通过线路与高压电源接地连接。本实用新型提供的毛细管电泳装置结构简单、操作简便且能提供精确进样。