您现在的位置: 首页> 研究主题> 放线菌酮

放线菌酮

放线菌酮的相关文献在1989年到2021年内共计86篇,主要集中在基础医学、畜牧、动物医学、狩猎、蚕、蜂、肿瘤学 等领域,其中期刊论文72篇、会议论文5篇、专利文献139076篇;相关期刊58种,包括中山大学学报(自然科学版)、深圳大学学报(理工版)、生物技术通报等; 相关会议5种,包括第六届中国畜牧科技论坛、第十五次全国动物遗传育种学术讨论会、中国科协第六届青年学术年会卫星会议-新疆第六届青年学术年会暨首届博士生论坛等;放线菌酮的相关文献由242位作者贡献,包括霍霞、惠国华、曾耀英等。

放线菌酮—发文量

期刊论文>

论文:72 占比:0.05%

会议论文>

论文:5 占比:0.00%

专利文献>

论文:139076 占比:99.94%

总计:139153篇

放线菌酮—发文趋势图

放线菌酮

-研究学者

  • 霍霞
  • 惠国华
  • 曾耀英
  • 朴英杰
  • 葛阳杨
  • 郑钢英
  • 黄行许
  • 狄静芳
  • 汤旭翔
  • 金姣姣
  • 期刊论文
  • 会议论文
  • 专利文献

搜索

排序:

年份

    • 曹涤环
    • 摘要: 抗菌素是微生物代谢过程中所产生的杀虫杀菌物质,具有抑制其他微生物的生长发育,以及阻止其生理机能的作用。至今为止,世界上已发现的抗菌素达到2000多种,大部分属于放线菌的分泌产物,其中有50余种用于医药卫生方面,称为医用抗菌素。随着研究的不断深入,目前已发现农用方面的抗菌素也越来越多,简称农用抗菌素,如使用比较多的是井冈霉素、内疗素、庆丰霉素、放线菌酮、灭瘟素、灰黄霉素等等。均是当前使用比较多的主要抗菌素品种。
    • 莫显刚; 洪伟; 王兰; 张莉; 刘大男; 代陆军; 蒋金; 郜双林
    • 摘要: 目的 探讨缺氧对细胞膜三磷酸腺苷结合盒转运体A1 (ABCA1)降解的影响及其与钙蛋白酶的相关机制.方法 肝X受体激动剂TO-901317刺激RAW264.7细胞24 h,实时定量聚合酶链反应检测ABCA1 mRNA 表达水平.生物素标记法提取细胞膜蛋白,Western blot检测细胞膜ABCA1蛋白水平表达.Western blot检测在放线菌酮存在或不存在条件下,TO-901317干预的RAW264.7细胞经过12h缺氧(1%氧气O2)处理后细胞膜ABCA1蛋白水平,Suc-LLVY-氨基虫荧光素法检测缺氧细胞内钙蛋白酶活性.RAW264.7细胞给予钙蛋白酶抑制剂ALLN干预,检测细胞膜ABCA1蛋白表达及钙蛋白酶活性.结果 TO-901317上调ABCA1 mRNA及细胞膜蛋白水平表达.无论放线菌酮存在或不存在情况下,缺氧均能降低细胞膜ABCA1蛋白水平,升高钙蛋白酶活性.钙蛋白酶抑制剂ALLN能部分逆转缺氧诱导细胞膜ABCA1蛋白水平降低.结论 缺氧可能通过增加钙蛋白酶活性,从而加速细胞膜ABCA1蛋白降解.%Objective To investigate the effect of hypoxia on the degradation of plasma membrane ATP binding cassette transporter A1 (ABCA1) and its calpain-related mechanism.Methods qRT-PCR was used to detect ABCA1 mRNA level in RAW264.7 cells stimulated by liver X receptor agonist TO-901317 for 24 h.Plasma membrane protein was extracted by the biotin labeling method of surface proteins,and membrane ABCA1 protein level was determined by Western blot.Western blot was applied to detect the plasma membrane ABCA1 protein level from TO-901317-treated RAW264.7 cells after 12-h hypoxia (1% O2) treatment in the presence or absence of Cycloheximide.Furthermore,the calpain activity in the hypoxic cells was assayed by the method of Suc-LLVY-aminoluciferin.Finally,plasma membrane ABCA1 protein and calpain activity were measured in the RAW264.7 cells after intervention with calpain inhibitor ALLN.Results LXR agonist TO901317 up-regulated ABCA1 mRNA and membrane protein levels.Hypoxia reduced plasma membrane ABCA1 protein in the RAW264.7 cells and increased the calpain activity in the absence or presence of Cycloheximide.Calpain inhibitor ALLN,in part,reversed the decrease of plasma membrane ABCA1 induced by hypoxia.Conclusions Hypoxia might accelerate the degradation of plasma membrane ABCA1 via enhancement of calpain activity.
    • 夏洪宇; 孔稳稳; 徐蕊; 苍炜; 李晶
    • 摘要: 研究蛋白质亚细胞定位的常规方法是构建由35S启动子驱动目的基因与绿色荧光蛋白基因(GFP)融合的表达载体,在细胞中瞬时或稳定表达来确定该蛋白质在细胞中的定位.35S启动子的优势是能够获得较强的GFP信号,但同时也可能因为蛋白质合成量过大,导致部分蛋白滞留在运输途径中或出现在其真实定位以外的区域.为了解决这一问题,以模式植物拟南芥中黄素单氧化酶FMOGS-OX1为例,利用蛋白质抑制剂放线菌酮处理过量表达FMOGS-OX1-GFP融合蛋白的烟草叶片.结果表明:未经放线菌酮处理的烟草叶片表皮细胞,细胞质和内质网中均呈现了较强的荧光信号,放线菌酮处理后,内质网上的信号消失,而细胞质则呈现出稳定的信号,因此判断FMOGS-OX1合成后可能是经过内质网运输到细胞质中的.上述结果证明适当的放线菌酮处理,能够避免强启动子驱动报告基因造成的蛋白质合成过量的问题,可有效地提高蛋白质亚细胞定位的准确性.%The conventional method of protein subcellular localization is to build the vector with the expression of fused target gene and green fluorescent protein gene(GFP)driven by 35S promoter. The subcellular localization of the target protein is then determined in the cells transiently expressing the fusion gene. Utilization of 35S promoter will lead to overexpression of the fusion gene and obtaining strong GFP signal. But sometimes the excessively synthesized protein will possibly remain in the transportation organelle or the areas exceeding the native protein location. The aim of this study is to solve this problem in the study of protein subcellular localization. To accurately determine the subcellular localization of Flavin-Containing Monooxygenase 1(FMOGS-OX1)in model plant Arabidopsis,a protein inhibitor,cycloheximide was applied to repress the over-expression of FMOGS-OX1-GFP fusion protein in tobacco epidermal cell. The results showed that before the treatment of cycloheximide,strong GFP signal was presented in both ER and cytosol. While after treated with cycloheximide,GFP signal disappeared in ER but remained in cytosol. This study demonstrated that proper treatment of cycloheximide may effectively avoid the excessive over-expression of the gene driven by 35S and thus is conducive to precisely determine the intercellular position where the protein facilitates its function.
    • 佟桂芝; 宋斌; 李信涛; 董正德; 海龙; 郭立宏; 王嘉厚
    • 摘要: 为了探讨放线菌酮(CHX)对卵母细胞预成熟的影响及囊胚滋养层细胞囊泡(TVS)、维生素C对肉羊体外胚胎质量的影响,试验采用从屠宰厂(场)收集的肉羊卵巢,抽取卵巢表面2 ~8 mm的卵泡卵母细胞进行体外成熟、体外受精和早期胚胎体外培养.结果表明:经过5%CHX预成熟的卵母细胞卵裂率、桑葚胚率、囊胚率显著高于对照组(P<0.05);在体外受精及早期胚胎培养液(SOFaa)中添加1%维生素C组与TVS共培养组的卵裂率和囊胚发育率均高于其他试验组(P<0.05);体外胚胎与TVS共移植受胎率显著高于对照组(P<0.05).说明核质同期成熟处理及抗氧化剂可以用于高效生产体外胚胎.
    • 佟桂芝1; 宋斌1; 李信涛2; 董正德1; 海龙1; 郭立宏1; 王嘉厚1
    • 摘要: 为了探讨放线菌酮(CHX)对卵母细胞预成熟的影响及囊胚滋养层细胞囊泡(TVS)、维生素C对肉羊体外胚胎质量的影响,试验采用从屠宰厂(场)收集的肉羊卵巢,抽取卵巢表面2~8 mm的卵泡卵母细胞进行体外成熟、体外受精和早期胚胎体外培养。结果表明:经过5%CHX预成熟的卵母细胞卵裂率、桑葚胚率、囊胚率显著高于对照组(P〈0.05);在体外受精及早期胚胎培养液(SOFaa)中添加1%维生素C组与TVS共培养组的卵裂率和囊胚发育率均高于其他试验组(P〈0.05);体外胚胎与TVS共移植受胎率显著高于对照组(P〈0.05)。说明核质同期成熟处理及抗氧化剂可以用于高效生产体外胚胎。
    • 杨华; 徐金柱; 秦长生; 揭育泽
    • 摘要: 文章比较了两性霉素、伊曲康唑和放线菌酮对土壤内金龟子绿僵菌(Metarhizium anisopliae)、黄绿绿僵菌(M.flavoviride)和6种非目标真菌生长的影响。结果表明,伊曲康唑对毛霉(Mucor racemosus)和黑曲霉(Aspergillus niger)的抑菌效果较差,浓度达200μg/mL时对毛霉的抑制率仅达54.84%,且对绿僵菌生长的抑制作用显著大于两性霉素和放线菌酮(P<0.01),不适宜用于土壤绿僵菌的分离。两性霉素和放线菌酮在浓度为100μg/mL时,对6种非目标真菌抑制率达69%以上,可作为绿僵菌分离的真菌抗生素。%The effects of 3 anti- fungal agents on the growth of Metarhizium anisopliae,M.flavoviride strains and 6 non-target fungis were studied.The results showed that itraconazole did not have good inhibitory effects on Mucor racemosus and Aspergillus niger,the rate of M.racemosus controlling was 54.84%,but the inhibi-tory effects on the growth of Metarhizium were significantly higher than that of amphotericin and cycloheximide (P<0.01),suggesting it was not suitable for Metarhizium isolation from soil.Amphotericin and cycloheximide of 100μg/mL could inhibit over 69%of 6 non-target fungis,and were the most suitable anti-fungal agents for Metarhizium isolation.
    • 莫蓓莘; 黄彪; 刘丽; 叶浩; 徐晓峰
    • 摘要: Although intensive studies have been conducted in seed germination, the exact requirements for germination is still poorly understood, particularly in terms of de novo RNA and protein syntheses. To find out if seeds can complete germination relying solely on stored mRNAs or stored proteins, transcription inhibitor a-amanitin and translation inhibitor cycloheximide were used to inhibit the synthesis of new mRNAs and new proteins of soybean radicles respectively. Germination of soybean radicles were observed after these treatments. Results show that, under the conditions of inhibition of transcription, soybean radicles are able to complete germination, but seedling growth stops. Under the conditions of the inhibition of translation, soybean radicles cannot complete germination. These results indicate that soybean radicle germination can rely on the mRNAs synthesized during seed development and stored in the dry seeds. The development of the seedlings cannot totally rely on stored mRNAs: newly synthesized mRNAs are required for this process. Also newly synthesized proteins are needed for soybean radicle germination. Fractionation of soybean radicle cell components by ultracentrifugation and quantification of mRNAs in different cell fractions by real-time PCR finds that mRNAs exist mainly in non-polysome structures in the dry seed while they exist mainly in the polysome after 4 hours of imbibition. This suggests that mRNAs are mainly in the state of storage in dry seeds. After imbibition, mRNAs move to polysome in the state of translation. Polysome profiles in different times of imbibition also reflect the movement of mRNAs during the germination process.%为研究种子是否能完全依靠储存的mRNA或储存的蛋白质完成萌发过程,分别利用转录抑制剂α-鹅膏蕈碱(α-amanitin)和制剂放线菌酮处理大豆胚根,抑制新的mRNA或新的蛋白质的合成,观察大豆胚根的萌发情况.结果发现,在转录抑制的条件下,大豆胚根能够完成萌发,种子萌发后,种苗生长停止;在翻译被抑制的条件下,大豆胚根不能完成萌发.说明大豆胚根萌发能够依赖发育过程合成并贮存在干种子中的mRNA;但种苗的发育不能完全依赖储存的RNA,需要新合成的mRNA参与.大豆胚根不能依赖储存蛋白质完成萌发过程.对大豆胚根细胞内容物进行超速离心并对不同组分中mRNA含量进行定量分析发现,干种子中的mRNA主要存在于多核糖体之外的细胞结构中,而在萌发过程中,mRNA主要存在于多核糖体中.这表明干种子中的mRNA主要以储存状态存在于mRNA的储存结构中,萌发过程大部分mRNA从储存结构中转移到多核糖体中变为活跃的翻译状态.不同萌发时期多核糖体的特征曲线也体现mRNA在细胞内存在位置的这种变化.
    • 摘要: 抗菌素是微生物代谢过程中所产生的杀菌物质,具有抑制其它微生物的生长发育,以及阻碍其生理机能的作用。迄今为止,世界上已发现的抗菌素有2000多种,大部分属放线菌的分泌产物,其中约50种用于医药卫生方面,称医用抗菌素;有10多种用于农业,称农用抗菌素简称农抗。如使用比较普遍的有链霉素、井岗霉素、内疗素、庆丰霉素、灰黄霉素、放线菌酮、灭瘟素等,都是当前应用较广的主要抗菌素品种。
    • 张军华; 陈丽丽; 李忠玉; 吴移谋
    • 摘要: 研究沙眼表原体(Ghlamydia trachomatis,Ct)血清型LGV L2在体外培养的繁殖规律及其影响因素,确定血清型LGV L2在体外培养的最佳生长发育条件.用沙眼衣原体血清型LGV L2分别感染HEp-2细胞、HeLa229细胞、HepG-2细胞、SGC-7901细胞和Vero细胞,在荧光显微镜下计数包涵体形成单位(inclusion figure unity,IFU)和观察培养不同时同后包涵体的形态,比较不同细胞对血清型LGV L2的敏感性及血清型LGVL2在不同细胞内的生长情况.同时分别设DEAE葡聚糖处理组与未处理组,含放线菌酮培养组与不含放线菌酮培养组,比较培养12、24、36和48 h后血清型LGV L2包函体形态、IFU和Real-Time PCR定量检测血清型LGV L2的核酸量,判断DEAE葡聚糖和放线菌酮对沙眼表原体血清型LGV L2生长的影响.在感染20h后,王微境下观察发现HEp-2、Vero、HepG-2、HeLa和SGC-7901细胞均不同程度肿胀,5种细胞内均可见包涵体,大约40 ~48 h后包涵体占据整个胞浆.IFU计数和Real-Time PCR结果王示5种细胞中HeLa细胞感染率最高,HepG-2细胞感染率最低,血清型LGV L2在HeLa细胞中生长速度最快.荧光显微镜下计数IFU,发现DEAE葡聚糖预处理组和对照组中血清型LGV L2的感染率和生长发育没有明显区别,而含放残菌酮培养组中各细胞内血清型LGV L2生长速度较对照组快,Real-Time PCR检测结果显示放线菌酮组各细胞内血清型LGVL2核酸量较对熙组高.血清型LGV L2在HeLa细胞中的感染率最高,DEAE葡聚糖时血清型LGV L2的感染没有明显影响,而体外培养时添加放残菌酮有利于血清型LGV L2的生长发育.%The propagation pattern and influential factors of trachoma (Cklamydia trackomatis) serotype LCV L2 in vitro were studied and confirmed the optimization of its growth conditions in vitro. Hep-2, HeLa229, HepG-21 SGC-7901 and Vero cells were respectively infected with C. Irachomalis LGV 12, then stained with fluorescent antibody, and inclusion bodies were observed under fluorescent microscope and counted the inclusion figure unity (IFU ) after different times' cultivation and compared the sensitivity of different cells to serotype LGV L2 and the growth of aero-type LGV L2 in different cells. Meanwhile, DEAE-dexlran treated group, untreated group, and cycloheximide containing group and cycloheximide non-containing group were set up respectively, then compared the shape of LGV L2 inclusion bodies, the quantity of IFU and the quantity of nucleic acid of serotype LGV L2 by Real-Time PCR quantity detection 12, 24, 36 and 48 h after cultivation to determine the effect of DEAE-dextran and cycloheximide on the growth of Chiamydia trachomatu serotype LGV L2. The results showed thai 20 h after the infection, it wu found undermicroscope that Hep-2, Vero, HepG-2,HeLa, and SGC-7901 and cells swelled in varying degree, and the inclusion bodies could be found in the five kinds of cell, and the entire cytoplasm was occupied with inclusion bodies about 40 - 48 h after (be infection. And the results of IFU counting and Real-Time PCR showed that HeLa cells were in the highest infection rate among the 5 cells, while HepC-2 cells were in the lowest infection rate, and serotype LCV L2 grew the fastest in HeLa cells. It was found IFU counting under fluorescent microscope that the infection rate and growth of serotype LCV L2 between DEAE-dextran pretreated group and the control group, while the infection rate and growth in cells of serotype LCV L2 containing cycloheximide cultured group grew faster than the control group and Real-Time PCR test results showed the content of nucleic acid of serotype LCV L2 in cells of cycloheximide group was higher than the-control group. Therefore, the infection rate of serotype LCV L2 in HeLa cells were the highest, DEAE dextran had no obvious effect on infection of serotype LGV L2, but conducive to the growth of serotype LGV L2 when cultured in vitro added cycloheximide.
    • 马红; 付博; 赵金凤; 李忠秋; 房庆昌; 仁亮; 刘娣
    • 摘要: 为研究放线菌酮( CHX)在猪卵母细胞孤雌激活和体细胞核移植中的作用效果,并验证其对胚胎发育的促进作用,实验对比体外成熟的猪卵母细胞经电激活后结合6-二甲氨基嘌呤(6-D)、细胞松弛素B(CB)或CHX激活后的孤雌囊胚率,及3种激活药物对体细胞核移植重构胚的发育能力的影响;在孤雌激活前用CHX预处理卵母细胞5 min,研究其对孤雌激活后胚胎发育能力的影响.结果表明:在孤雌激活过程中,CHX在激活8h条件下囊胚率为48.0%,而CB和6-D处理4h即可达55.2%和63.3%.而在体细胞核移植重构胚的激活中,采用CHX进行激活囊胚率为19.6%,高于CB的10.5%和6-D的16.5%,各组间差异显著(P>0.05).孤雌激活前,卵母细胞经CHX预处理15 min可以提高CB或CHX激活的孤雌囊胚率.结果说明,CHX在孤雌激活能力较差,但对胚胎发育具有一定保护和促进作用.
  • 查看更多

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号