内皮依赖性舒张功能

摘要： 目的:观察麝香保心丸联合辛伐他汀治疗冠状动脉粥样硬化性心脏病(冠心病)合并高脂血症患者的效果.方法:选取86例冠心病合并高脂血症患者作为研究对象,按随机数字表法分为研究组(n=43)和对照组(n=43).对照组采用辛伐他汀胶囊治疗,研究组在对照组基础上联合麝香保心丸治疗,比较两组临床疗效、治疗前后心脏功能指标[左室射血分数(LVEF)、左室收缩期末内径(LVESD)]水平、血脂指标[总胆固醇(TC)、三酰甘油(TG)]水平、内皮依赖性舒张功能水平和不良反应发生率.结果:研究组治疗总有效率为95.35％,高于对照组的81.40％,差异有统计学意义(P0.05).结论:麝香保心丸联合辛伐他汀治疗冠心病合并高脂血症患者可提高治疗总有效率和内皮依赖性舒张功能水平,改善心脏功能指标水平,以及降低血脂指标水平,其效果优于单纯辛伐他汀胶囊治疗.

摘要： 目的 观察沙库巴曲缬沙坦治疗老年缺血性心肌病的疗效及安全性.方法 选取我院2017年8月—2018年9月收治老年缺血性心肌病病人80例,采用随机数字表法分为对照组和观察组,每组40例.对照组在常规治疗基础上给予马来酸依那普利治疗,观察组在常规治疗基础上给予沙库巴曲缬沙坦治疗,比较两组治疗前后血压、血脂、超声心动图指标、B型尿钠肽(BNP)、6 min步行试验(6MWT)、肱动脉内皮依赖性舒张功能(FMD)、颈动脉内膜中层厚度(CIMT)水平及不良反应发生率.结果 两组治疗后血压和血脂指标水平均明显低于治疗前(P0.05);观察组治疗后左室射血分数、左室舒张末内径、BNP及6MWT水平明显优于对照组(P0.05).结论 相较于马来酸依那普利,沙库巴曲缬沙坦治疗老年缺血性心肌病可有效改善心脏功能,提高运动耐力,增强血管扩张功能,且并未加重药物不良反应,而这一疗效优势独立于降压、调脂作用.

摘要： 目的:通过观察大鼠肺动脉环张力及肺小动脉形态学变化,探讨肺动脉高压发病机理及妊娠对肺动脉高压疾病进展影响的潜在机制.方法:雌性,6周龄,SD大鼠55只,随机分为四组:空白对照组(n=10);野百合碱组(MCT,n=13);妊娠组(n=12);妊娠MCT组(n=20).动物模型[1]完成后经颈静脉置管及股动脉置管采集血流动力学数据,并取大鼠三级肺动脉环,观察ACh对各组大鼠肺动脉环舒张作用;同时采集肺组织进行HE染色,观察肺小动脉损伤情况.结果:与MCT相比,妊娠MCT组MPAP显著降低(P＜0.05);MCT组ACh诱导的内皮依赖性动脉舒张率比空白对照组显著降低(P＜0.05);妊娠MCT组ACh诱导的内皮依赖性动脉舒张率比妊娠组及MCT组均显著降低(P＜0.05);妊娠组与空白对照组大鼠肺血管内皮依赖性舒张功能无显著性差异(P＞0.05);MCT组、妊娠MCT组大鼠肺动脉壁及肺小动脉弹性中膜厚度增加,内径逐渐变窄,肺小动脉有微血管瘤形成,部分小血管明显闭塞;妊娠MCT组较MCT组增厚肺小动脉密度明显增加(P＜0.05).结论:妊娠会降低肺高压大鼠肺动脉内皮依赖性舒张功能,促进肺小动脉损伤,加重肺动脉高压.

摘要： 目的:探讨脑蛋白水解物对缺血性脑血管病患者血管内皮依赖性舒张功能(FMD)水平及血液流变学的影响.方法:选择某院收治的缺血性脑血管病患者86例,采取前瞻性研究,将患者根据随机数字表法分为2组,每组各43例.2组患者均接受基础治疗,在此基础上,予以对照组患者血塞通注射液治疗,予以观察组血塞通注射液联合脑蛋白水解物注射液治疗.治疗4个疗程后,比较2组FMD水平、血液流变学、神经功能缺损评分(NIHSS),并记录治疗期间不良反应发生情况.结果:治疗后,观察组FMD水平高于对照组,差异具有统计学意义(P<0.05);观察组全血低切黏度、全血高切黏度、血浆纤维蛋白原水平和血浆黏度均低于对照组,差异具有统计学意义(P<0.05);观察组NIHSS评分明显低于对照组,2组比较差异有统计学意义(P<0.05);治疗期间,2组患者均未出现明显的不良反应.结论:对缺血性脑血管病患者实施脑蛋白水解物治疗,能有效改善患者血液流变学各项指标和神经功能缺损程度,提高FMD水平,值得临床应用.

摘要： Objective To investigate the effects of telmisartan on oxidative stress and vascular endothelial function in patients with coronary slow flow (CSF).Methods One hundred and twenty six patients with CSF and normal coronary angio-graphy were selected. All the patients were randomly divided into conventional treatment group and telmisartan group, 63 cases in each group. Patients in conventional treatment group were given conventional treatment (aspirin, nitrates and atorvastatin), while patients in the telmisartan group were given telmisartan on the basis of conventional treatment. TIMI, malondialdehyde (MDA);reactrve oxygen species (ROS);superoxidc dismutase(SOD),endothelial dependent flow mediated vasodilation (FMD), endothelin 1 (ET-1), nitric oxide (NO) were observed in the two groups before and after eight weeks of treatment. Results TIMI reduced significantly in the telmisartan group after treatment compared with before treatment and conventional group after treatment(t=7.384,t=6.932,P=0.001,P=0.004). SOD, FMD, NO improved in the telmisartan group after treatment compared with before treatment(t=5.631,t=5.922,t=5.691,P=0.00,P=0.000,P=0.002), MDA, ROS, ET 1level decreased (t=6.462,t=7.281,t=6.970,P=0.001,P=0.004,P=0.005). TIMI, SOD, MDA, ROS, FMD, NO, ET-1 of conventional treatment had no significantly changes before and after treatment(t=0.471,t=0.722,t=0.350,t=0.193,t=0.873,t=0.554,t=0.381,P=0.530,P=0.568,P=0.466,P=0.357,P=0.580,P=0.457,P=0.371). The post treatment of SOD,FMD, NO in telmisartan group were significantly increased with conventional group(t=4.064,t=5.091,t=4.270,P=0.000,P=0.017,P=0.007), TIMI, MDA, ROS, ET-1 levels in telmisartan group were significantly reduced with conventional group(t=6.932,t=4.181,t=4.360,t=7.944,P=0.004, P=0.002, P=0.007, P=0003). Conclusion The telmisartan group curative effect is significantly better than that of conventional group in the treatment of CSF, telmisartan may play a role in the mechanism of inhibition of oxidative stress response and improving the vascular endothelial function.%目的 观察替米沙坦对冠状动脉慢血流(CSF)患者氧化应激和血管内皮功能的影响.方法 选取2015年8月-2016年8月山东省济宁市第一人民医院心血管诊疗中心因胸痛行冠状动脉造影检查确诊CSF患者126例作为研究对象,随机分为对照组和观察组,每组63例.对照组给予拜阿司匹林、硝酸酯类、阿托伐他汀等常规治疗;观察组在常规治疗的基础上加用替米沙坦治疗.观察治疗前、治疗8周2组间及组内患者冠状动脉血流TIMI计帧、丙二醛(MDA)、活性氧(ROS)、超氧物歧化酶(SOD)及肱动脉血流介导内皮依赖性舒张功能(FMD)、血浆内皮素-1(ET-1)、一氧化氮(NO)水平的变化.结果 观察组心绞痛症状改善总有效率为92.06%高于对照组的61.90%(P0.05).治疗后观察组SOD、FMD、NO均较对照组升高(t=4.064、5.091、4.270,P<0.01),且TIMI计帧、MDA、ROS、ET-1水平较对照组降低(t=6.932、4.181、4.360、7.944,P<0.01).结论 替米沙坦治疗CSF优于对照组,替米沙坦可能通过抑制机体氧化应激反应、改善血管内皮功能的机制发挥作用.

摘要： Objective: To investigate efficacy of valsartan hydrochlorothiazide on the thickness of carotid intima-media in patients with mild to moderate essential hypertension. Methods: A total of 244 patients with mild to moderate essential hypertension were enrolled in the study. The patients were divided into two groups: observation group and control group (122 cases). The patients in the control group were treated with oral hydrochlorothiazide, The patients in the observation group were treated with oral valsartan hydrochlorothiazide. The patients in the two groups were treated continuously for 6 months. The blood pressure before and after treatment were compared between the two groups. The brachial artery endothelium-dependent diastolic function (EDD), carotid artery intima-media thickness (IMT) were compared. Results: After treatment, the blood pressure levels of the two groups were significantly lower than those before treatment (P0.05). Compared with the control group, the EDD and IMT levels were significantly improved (P>0.05). The EDD of the observation group was significantly higher than that of the control group (P0.05);对照组治疗后与治疗前相比EDD及IMT水平均无显著改善(P>0.05),观察组治疗后与治疗前相比EDD明显增高,IMT明显降低,差异具有统计学意义(P<0.05),与同期对照组相比差异具有统计学意义(P<0.05).结论:缬沙坦/氢氯噻嗪用于轻中度原发性高血压患者的治疗能够有效控制血压,并改善肱动脉内皮功能,减少颈动脉内膜中层厚度,值得在临床上推广和应用.

摘要： Objective To investigate the effects of all-trans-retinoic acid ( ATRA ) on endothelial-dependent dila-tion in experimental atherosclerosis rabbits. Methods New Zealand white rabbits were randomly divided into 3 groups:the normal group were fed on standard diet;high-fat diet model group were fed on high-fat diet ( standard diet supplemented with 1℅ cholesterol+5℅ lard);ATRA group were fed on high-fat diet and 5 mg/( kg·day) administered orally. After 2, 4, 8, 12 weeks, experimental rabbits were sacrificed to get blood and blood lipids were tested with a kit;the formation of lipid plaque was observed by oil red staining and the change of endothelial structure was watched by HE staining. After 8 weeks, 3 rabbits of each group were randomly assigned to detect en-dothelial-dependent dilation and non-endothelial-dependent dilation on isolated arterial rings. Results Compared with the normal group, after 2 weeks, the concentration of total cholesterol( TCH) and LDL-C in model group in-creased significantly (P<0. 05). The concentration of triglyceride(TG) and HDL-C increased significantly after 4 weeks (P<0. 05);Oil red staining showed that in two weeks, lipid plaque was not obvious;4 weeks later, the lipid plaque was increased;HE staining showed that the intima was thickened with the passage of time; the foam cells increased;the vascular smooth muscle cell polarity was disordered, and migrating to the intima;the change of EDD indicated that the model group attenuated conspicuously (P<0. 05). Compared with the model group, in AT-RA group, there was no difference in the concentration of TG. The concentration of TCH decreased significantly ( P<0. 05 );the concentration of HDL-C for 4 weeks and 12 weeks significantly increased ( P<0. 05 ) . The level of LDL-C for 2 weeks and 12 weeks reduced ( P<0. 05 ); Oil red staining revealed that the lipid plaque was visible after 4 weeks;HE staining indicated that compared with the model group, the intima of ATRA group was slightly thickened, foam cells were reduced, and arrangement of the smooth muscle cells was more neatly;when the level of Ach was 10 -7 mol/L, the EDD of ATRA group was significantly higher than that in the model group ( P <0. 05). Conclusion Hyperlipidemia is a cause of atherosclerosis. The damaged artery endothelium makes endo-thelial-dependent dilation decreased, and ATRA might improve endothelium dependent relaxing function by reduc-ing serum lipids.%目的 探讨全反式维甲酸(ATRA)对实验性动脉粥样硬化兔动脉血管内皮依赖性舒张功能的影响.方法 普通级新西兰大耳白兔随机分为3组,正常组:饲喂普通饲料;模型组:饲喂高脂饲料(普通饲料+1℅胆固醇+5℅猪油);ATRA组:饲喂高脂饲料,同时ATRA灌胃5 mg/(kg·d).分别于2、4、8、12周后处死取血,检测血清中血脂指标;油红染色观察大体斑块形成情况;HE染色观察动脉的内皮结构变化;8周后,每组随机取3只实验兔进行离体动脉环实验检测兔动脉内皮依赖性舒张功能(EDD)及非内皮依赖性舒张功能(NEDD).结果 与正常组相比,2周后,模型组血清中总胆固醇(TCH)、低密度脂蛋白-胆固醇(LDL-C)浓度显著升高(P<0.05);4周后,三酰甘油(TG)、高密度脂蛋白-胆固醇(HDL-C)浓度明显升高(P<0.05);油红染色表明2周时脂质斑块不明显,4周以后脂质斑块明显;HE染色显示内膜增厚,泡沫细胞增多,平滑肌细胞极性紊乱,向内膜迁移;内皮依赖性舒张功能高脂组EDD显著低于正常组(P<0.05).与模型组相比,ATRA治疗组血清中TG浓度无差异,TCH浓度明显下降(P<0.05),HDL-C浓度4周及12周时明显升高(P<0.05),LDL-C浓度2周及12周时降低(P<0.05);油红染色表明4周后可见脂质斑块;HE染色显示内膜稍微增厚,泡沫细胞减少,平滑肌细胞排列较整齐;内皮依赖性舒张功能检测表明当乙酰胆碱浓度为10-7 mol/L时,ATRA组EDD显著高于高脂组(P<0.05).结论 血脂升高可引起动脉粥样硬化的发生,动脉内皮损伤使内皮依赖性舒张功能下降,ATRA可能通过降低血脂改善内皮依赖性舒张功能.

摘要： 目的:探讨血清尿酸水平对冠状动脉痉挛的影响及机制.方法:冠状动脉造影提示冠状动脉无显著狭窄的胸痛患者58例,分为痉挛组(30例)和对照组(28例).收集两组患者临床资料,检测患者血清尿酸及内皮依赖性舒张功能(flow-mediated dilation,FMD).结果:两组临床基本资料对比差异无统计学意义,痉挛组血清尿酸水平高于对照组[(351.6±93.7) μmol/L vs (316.9±80.6) μmol/L,P＜0.05],FMD低于对照组(4.08％±0.61％ vs 7.17％±1.29％,P＜0.05).直线相关回归分析提示血清尿酸水平与FMD呈负相关(r=-0.398,P＜0.05).结论:冠状动脉痉挛患者血清尿酸水平升高,导致血管内皮功能减退,可能促进冠状动脉痉挛的发生.

摘要： Objective To investigate the effect of fasudil on vascular endothelial function in patients with coronary slow flow( CSF) . Methods Eighty⁃two patients with CSF and normal coronary angiography were selected and randomly divided into conventional treatment group and fasudil group, 41 cases in each group. Patients in conventional treatment group were given conventional treatment( aspirin,nitrates and atorvasta⁃tin) ,while patients in the fasudil group were given fasudil on the basis of conventional treatment. The angina pectoris,TIMI,endothelial⁃dependent flow⁃mediated vasodilation( FMD) ,the levels of plasma nitric oxide( NO) , endothelin⁃1( ET⁃1) and Rho kinase( ROCKI) of the brachial artery were observed in the two groups before and after two weeks of treatment. Results The total effective rate of fasudil group was 87. 80%,higher than that of conventional treatment group of 65. 85%,the difference was significant(χ2=68. 176,P<0. 05) . TIMI,FMD im⁃proved in the fasudil group after treatment compared with before treatment, the difference was significant ( t =4. 37,4. 43;P<0. 05);plasma NO level increased compared with before treatment(t=5. 63,P<0. 01),while ROCKI,ET⁃1 level decreased(t=6. 19,5. 66;P<0. 01). Plasma NO,ET⁃1,ROCKI and FMD,TIMI of conven⁃tional treatment had no significantly changes before and after treatment(P<0. 05). The post⁃treatment of NO, FMD,TIMI levels in fasudil group were significantly increased compared with conventional group ( ( 36. 17 ±7. 64) μmol/L vs. (24. 99±8. 96) μmol/L,(9. 96±1. 76)% vs. (5. 86±1. 45)%,17. 53±5. 81 vs. 29. 71 ±7. 83;t=4. 06,4. 18,5. 41;P<0. 05),while ROCKI,ET⁃1 levels in fasudil group were significantly decreased compared with conventional group((19. 57±1. 33) μg/L vs. (34. 38±1. 51) μg/L,(14. 36±6. 05) ng/L vs. (20. 95±6. 57) ng/L;t=3. 87,4. 36,P<0. 01). Conclusion Fasudil can significantly improve the vascular en⁃dothelial function in patients with CSF.%目的：探讨盐酸法舒地尔对冠状动脉慢血流（ CSF）患者血管内皮功能的影响。方法选择冠状动脉造影结果正常但是存在CSF现象的患者82例，按照随机的原则分为常规治疗组与法舒地尔组，每组各41例。常规治疗组给予拜阿司匹林、硝酸酯类、他汀等常规治疗；法舒地尔组在常规治疗的基础上加用盐酸法舒地尔治疗。观察治疗前、后2周两组之间及组内患者心绞痛疗效、冠状动脉血流速度（TIMI）、肱动脉血流介导内皮依赖性舒张功能（FMD）、血浆一氧化氮（NO）、内皮素1（ET⁃1）及Rho激酶I（ ROCKI）水平的变化。结果法舒地尔组治疗后患者心绞痛疗效总有效率为87．80％，常规治疗组总有效率为65．85％，差异有统计学意义（χ2＝68．176，P＜0．05）。法舒地尔组治疗后患者TIMI计帧、FMD均显著高于治疗前，差异均有统计学意义（ t值分别为4．37、4．43，P均＜0．05）；法舒地尔组患者治疗后血浆NO水平显著高于治疗前，差异有统计学意义（ t＝5．63，P＜0．01）；ROCKI、ET⁃1均显著低于治疗前，差异均有统计学意义（t 值分别为6．19、5．66，P 均＜0．01）。常规治疗组治疗后 NO、ROCKI、ET⁃1水平及FMD、TIMI计帧与治疗前相比，差异均无统计学意义（ P 均＜0．05）。法舒地尔组治疗后 NO [（36．17±7．64）μmol／L与（24．99±8．96）μmol／L]水平及FMD[（9．96±1．76）％与（5．86±1．45）％]、TIMI计帧（17．53±5．81与29．71±7．83）均较常规治疗组治疗后显著升高（t 值分别为4．06、4．18、5．41，P 均＜0．05），ROCKI[（19．57±1．33）μg／L 与（34．38±1．51）μg／L]、ET⁃1[（14．36±6．05） ng／L 与（20．95±6．57） ng／L]水平较常规治疗组治疗后显著降低（ t值分别为3．87、4．36，P均＜0．01）。结论盐酸法舒地尔能够显著改善CSF患者的血管内皮功能。

摘要： 目的：探讨重症肌无力患者内皮依赖性舒张功能的变化。方法 采用高分辨率彩色多普勒超声仪,检测42例重症肌无力患者肱动脉内皮依赖性舒张功能,并与健康对照组45例进行比较。结果 重症肌无力患者肱动脉内皮依赖性舒张功能较健康对照组明显降低。结论 重症肌无力患者有内皮依赖性舒张功能的损害。

摘要： 本发明涉及一种用于痰液核酸的解旋酶依赖性等温扩增添加剂及解旋酶依赖性等温扩增方法，所述的添加剂由甜菜碱、二甲基亚砜、乙二醇二乙醚二胺四乙酸混合而成，所述的甜菜碱、所述的二甲基亚砜、所述的乙二醇二乙醚二胺四乙酸的物质的量比为0.4~2:0.8~3.4:1。应用本发明中的添加剂及扩增方法，可以减少痰液样本DNA对HDA的抑制作用，成功扩增痰液DNA中的目的基因片段，有效避免假阴性结果，可用于临床检测。

摘要： 用于治疗癌症的CDK4/6抑制剂和mTOR抑制剂的组合。

摘要： 本发明公开了一种通过在存在pH依赖性表面活性剂的情况下使含氟聚合物分散体与阴离子交换树脂接触来降低所述含氟聚合物分散体中氟化乳化剂的量的方法，本发明还公开了包含所述pH依赖性表面活性剂的含氟聚合物分散体及其使用。

摘要： 本发明涉及具有非FcγR依赖性激动活性的肿瘤坏死因子(TNF)受体超家族(TNFRSF)受体‑激活抗体融合蛋白，及其组合物和方法。

摘要： 本发明涉及一种用于痰液核酸的解旋酶依赖性等温扩增添加剂及解旋酶依赖性等温扩增方法，所述的添加剂由甜菜碱、二甲基亚砜、乙二醇二乙醚二胺四乙酸混合而成，所述的甜菜碱、所述的二甲基亚砜、所述的乙二醇二乙醚二胺四乙酸的物质的量比为0.4~2:0.8~3.4:1。应用本发明中的添加剂及扩增方法，可以减少痰液样本DNA对HDA的抑制作用，成功扩增痰液DNA中的目的基因片段，有效避免假阴性结果，可用于临床检测。

摘要： 用于治疗癌症的CDK4/6抑制剂和mTOR抑制剂的组合。

摘要： 本发明公开了一种通过在存在pH依赖性表面活性剂的情况下使含氟聚合物分散体与阴离子交换树脂接触来降低所述含氟聚合物分散体中氟化乳化剂的量的方法，本发明还公开了包含所述pH依赖性表面活性剂的含氟聚合物分散体及其使用。

摘要： 本发明涉及用于活性成分非pH依赖性缓释的固体药物制剂，其包含至少一层的一种或多种水不溶性聚合物、至少一层的一种或多种pH依赖性水溶性聚合物和含活性成分的核芯，所述核芯具有高pH依赖性水溶解度并含有至少一种渗透剂。

摘要： 本发明提供一种阻滞激素依赖性癌症向激素非依赖性癌症转化的试剂,其包含一种激素药物。上述试剂显示了有效阻滞激素依赖性癌症向激素非依赖性癌症转化的效应。

摘要： 本发明涉及一种检测弱氧化低密度脂蛋白损伤小鼠肠系膜动脉内皮依赖性舒张功能及机制的方法，采用离体微血管张力描记，透射电镜观察血管内皮超微结构。结果显示mmLDL致小鼠肠系膜动脉内皮细胞明显水肿、内弹力膜断裂，显著抑制EDHF介导的舒张；显著抑制NO介导的舒张；对PGI