摘要:
Essential oil was extracted from fresh samples of Mentha piperita grown in Ruili, Yunnan by using the steam distillation method, and its volatile chemical constituents were analyzed and identified by gas chromatography-mass spectrometry (GC-MS). The chromatograms obtained by mass spectrometry were qualitatively and quantitatively analyzed by using the mass spectrometry workstation Xcalibur3.0, compared with the data retrieved from NIST08 standard mass spectrometry database, and resorted to comprehensive analysis. From the comprehensive analysis 43 major volatile compounds were identified, and their mass fractions were calculated by using the peak area normalization method. These compounds accounted for 97.30% of the total components of the volatile oil, of which 7 components had higher content in the essential oil,i.e.menthone(14.01%),menthol(53.80%),lauraldehyde (4.83%),tetradecanol (3.05%), alpha methyl aldehyde (2.66%), terppinolene (2.54%), and 4-acetic acid, -1-, terpene, alkenyl (2.58%), and accounted for 83.47% of the total volatile oil. The major constitutes and content of the volatile oil extracted from M. piperita varied with the planting location and growing conditions for the plants,which determined the difference in aroma quality of the essential oil.This provides scientific basis for further research and development of M.piperita grown in Ruili,Yunnan Province.%采用水蒸气蒸馏法从瑞丽市种植的椒样薄荷鲜样中提取精油,利用气相色谱-质谱联用仪(GC-MS)对精油的挥发性化学成分进行分析鉴定,各挥发性化学成分经质谱扫描后得到的色谱图通过质谱工作站Xcalibur3.0进行定性和定量分析,结合NIST08标准质谱图数据库对比检索,并对图谱进行综合分析,鉴定出43种主要的挥发性化学成分,按峰面积归一化法计算各成分在精油中的相对质量分数.43种挥发性化学成分占挥发油总量的97.30%,其中含量较高的7个成分分别是薄荷酮(14.01%)、薄荷醇(53.80%)、月桂醛(4.83%)、肉豆蔻醇(3.05﹪)、α-甲基戊醛(2.66%)、红樟油(2.54%)和4-乙酸基-1-萜品烯(2.58%),占挥发油总量的83.47%.这些主要成分的种类和含量差异因地域和种植条件的不同,决定了椒样薄荷精油的香气品质差异.该研究为进一步研究和开发瑞丽本地种植的椒样薄荷提供了科学依据.