rCBF

摘要： 目的 观察醒脑开窍针刺法在改善缺血缺氧性脑病患者临床指标水平,提高患者日常生活能力方面的作用.方法 选取116例缺血缺氧性脑病患者,按照随机数字表法分为针刺组和对照组各58例.对照组采用常规的缺血缺氧性脑病治疗方法,针刺组在对照组的基础上采用醒脑开窍针刺法进行治疗.采用电解式组织血流计测定患者局部脑血流量(rCBF),生化检测静脉血血浆肌酸激酶同工酶BB (CK-BB)含量.采用Barthel指数评价法对患者的日常生活能力进行评价.结果 干预前两组患者rCBF数据差异无统计学意义(P＞0.05),干预后针刺组患者rCBF值为(183.57±17.26) mL/100[100 (g·min)]明显高于对照组(168.25±15.21) mL/100(g·min),差异具有统计学意义(P＜0.05).干预前两组患者CB-BB数据差异无统计学意义(P＞0.05),干预后针刺组患者CK-BB值为(172.24±13.69) U/L明显低于对照组(219.42±24.41) U/L,差异具有统计学意义(P＜0.05).干预后针刺组日常生活能力为(83.49±10.17)分明显高于对照组(72.14±11.37)分,差异具有统计学意义(P＜0.05).干预后针刺组认知功能评分为(29.29±2.32)分明显高于对照组(23.38±2.10)分,差异具有统计学意义(P＜0.05).结论 醒脑开窍针刺法能够有效地改善缺血缺氧性脑病患者rCBF、CK-BB水平,提高患者日常生活能力和认知功能.

摘要： 目的 观察参知健脑胶囊对大鼠局部脑血流量的影响.方法 将60只SD大鼠随机分为假手术组,模型组,金纳多组,参知健脑胶囊低、中、高组,每组10只.将大鼠囟门处颅骨磨薄,然后采用结扎双侧颈总动脉建立低灌注模型,术后10 min,除假手术组和模型组给予等体积生理盐水外,其余各组直肠注射给药,分别给予金纳多20 mg/kg以及参知健脑胶囊40、120、360 mg/kg.应用激光多普勒血流仪检测各组给药后30、60、90、120 min时点动物rCBF变化.结果 模型组术后rCBF下降明显,与假手术组相比有显著统计学差异(P0.05).结论 参知健脑胶囊抑制脑缺血大鼠rCBF的疗效与金纳多相似,其治疗血管性痴呆的作用可能与其改善脑部缺血状态有关.%Objective To observe the effect of Shenzhi Jiannao Capsules on local cerebral blood flow in rats. Methods 60 SD rats were randomly divided into sham-operated group, model group, Ginaton and Shenzhi Jiannao Capsules low, middle, high dosage groups, 10 rats each group. First, grinding the skull on fontanelle to make it thinner, then using bilateral comman carotid artery occlusion to establish hypoperfusion model. 10 minutes after the operation, except sham-operated group and model group were given equal volume of normal saline, the other groups were given Ginaton 20mgkg and Shenzhi Jiannao Capsule 40mg/kg、120mg/kg、360mg/kg by intrarectal administration. Laser Doppler Flowmetry was used to detect rCBF of each group. Results After operation, rCBF of model group was dramaticly decreased compared to sham-operation group and had significant statistical difference (P 0.05). Conclusion Shenzhi Jiannao Capsules had similar effect as Ginaton in inhibiting the decrease of rCBF of ischemic rats, and its efficacy on vascular dementia may be related to the improvement of brain ischemia state.

摘要： 目的:通过研究磁共振灌注成像(PWI)注射流率对脑各感兴趣区(ROI)相对脑血容量(r CBV)、相对脑血流量(r CBF)、平均通过时间(MTT)的影响,以探讨最佳注射流率.方法:选取医院神经内科短暂性脑缺血(TIA)PWI检查病人150例,以3、4、5mL/s注射流率分为A、B、C组,各50例,分析脑各感兴趣区峰值时间(time to peak,TTP)与r CBV、r CBF、MTT的关系.结果:各感兴趣区r CBV、r CBF由高至低分别为B、A、C组,P<0.05.MTT延迟程度由大至小分别为C、A、B组,P<0.05.A、B组对比剂局部外渗发生率明显低于C组,P<0.05.TTP与r CBV、r CBF呈正相关,MTT呈负相关(P<0.05).结论:PWI中采用4mL/s注射流率有助于产生良好的静脉团注效果,不仅满足诊断需求,且降低对比剂局部外渗风险,值得临床推广应用.%Objective: Through studying the effect of injection flow rate by magnetic resonance perfusion weighted imaging (PWI) in various brain region of interest (ROI) for relative Cerebral Blood Volume (r CBV), relative Cerebral Blood Flow (r CBF) and Mean Transit Time (MTT), to discuss the best injection flow rate. Methods: 150 cases transient ischemic attack (TIA) PWI patients in neurology department of our hospital were selected, as 3, 4, 5 mL/s injection flow rate were divided into A, B, C groups, each for 50 cases, the relative between brain region of interest r CBV, r CBF, MTT and TTP were analyzed. Results: The various brain ROI r CBV, r CBF from high to low were B, A, C group, P<0.05. MTT delayed degree from large to small were C, A, B group, P<0.05. Local contrast agent extravasation rate of A, B group were lower than C group, P<0.05. TTP had positive correlation with r CBV, r CBF, negative with MTT, P<0.05. Conclusions: Giving 4 mL/s injection flow rate during PWI helps creating good venous injection effect, not only meets diagnosis demand, but also reduces local contrast agent extravasation rate, it is worthy of clinical popularization and application.

摘要： 目的:观察金匮通脉方联合阿替普酶溶栓治疗对溶栓时间窗外急性脑梗死区域组织灌注的影响.方法:将50例溶栓时间窗外6～72 h急性脑梗死患者采用随机数字表法随机分为两组,两组均给予抗血小板、调脂、调控血压和血糖等常规治疗.对照组25例采用阿替普酶溶栓治疗;治疗组25例在对照组治疗基础上加服金匮通脉方(黄芪、全瓜萎、薤白、益母草、丹参、山楂、制半夏),每日1剂,水煎服.两组均治疗14 d后判定疗效.结果:两组治疗后脑梗死中心区域CBV、CBF均较治疗前增加,MTT较治疗前缩短,但治疗组改善程度明显优于对照组(P＜0.01);治疗组治疗后脑梗死周围区域CBV、CBF均较治疗前增加(P＜0.01),且治疗组改善程度明显优于对照组(P＜0.01);治疗组治疗后脑梗死中心和周围区域的rCBV、rCBF均较治疗前明显增加(P＜0.01).结论:金匮通脉方联合阿替普酶溶栓治疗溶栓时间窗外急性脑梗死能够改善患者脑梗死中心及其周围区域组织灌注,尤其对梗死周围组织灌注的改善较为明显,值得进一步研究和运用.

摘要： Deficits in facial emotion processing are features of mild Alzheimer’s disease (AD). These impairments are often dis-tressing for carers as well as patients. Such non-cognitive symptoms are often cited as a contributing reason for admis-sion into institutionalised care. The ability to interpret emotional cues is crucial to healthy psychological function and relationships and impaired emotional facility may lead to antisocial behavior. Understanding the origins of the non-cognitive aspects of AD may lead to an improvement in the management of sufferers and ease the carer burden. In a cross-sectional study we recorded patients’ facial processing abilities, (emotion and identity recognition) and disease severity (ADAS-cog, Neuropsychiatic Inventory) and investigated the regional cerebral blood flow correlates of facial emotion processing deficits using 99Tcm HMAPO rCBF SPECT. Using statistical parametric mapping (SPM) we iden-tified decreased blood flow in posterior frontal regions specifically associated with emotion perception deficits. Non-emotional facial processing abilities or disease severity. The posterior frontal lobe has been identified in previous stud-ies in the absence of dementia as being important in emotion processing. The results suggest that the cognitive disease severity, in combination with the facial processing ability, do not completely explain facial emotion processing in AD patients and that the posterior frontal lobe mediates such behaviour.

摘要： s To study the effect of special brain area regional cerebral blood flow (rCBF) abnormal perfusion on learning and memory function and its molecular mechanism,64 adult male healthy Spragne-Dawley (SD) rats were randomly divided into two groups,the false operation group (control group) and the operation group (model group).After surgical operation,the operation group undertook bilateral common carotid artery permanent ligation,while the other group did not.Learning and memory function were measured by Y-maze at 4 h,8 h,24 h and 3 d after surgical operation,respectively.The rCBF of the right frontal lobe and hippocampus was also detected by the PerifluxPF model laser Doppler flowmetry,and the expressions of c-fos or c-jun or Bcl-2 and Bax were also measured by immune histochemistry S-P method accordingly.Results showed that the rCBF of the right frontal lobe and hippocampus in the operation group was significantly lower than that in the false operation group (P ＜ 0.05).The learning indexes,error number (EN),day of reach standard and total reaction time (TRT) in the operation group,were significantly higher than that in the false operation group (P＜ 0.05).However,the initiative evasion rate in the operation group was significantly lower than that in the false operation group.The study also found that the rCBF was relatively more,the indexes (EN,the day of reach standard and TRT) relatively fewer,but the initiative evasion rate and the memory keeping rate were relatively more.The positive expression and the average absorbency of Fos and Jun in the operation group were significantly higher than that in the false operation group (P＜ 0.05).Furthermore,Bax and Bcl-2 positive cells were all increased over time in the operation group,and the expression ratio of Bax/Bcl-2 in the operation group was significantly higher than that in the false operation group (P＜0.01).In conclusion,rCBF decrease can impair the learning and memory function in rats,which may be related to the increase of the expression ratio of c-fos or c-jun or Bcl-2 or Bax in the frontal cortex and hippocampus.

摘要： 目的 探讨两种蛛网膜下腔出血模型所致脑血管痉挛的情况.方法 选用健康Wistar大鼠36只,随机分为对照组、一次注血组、二次注血组.对照组注入生理盐水0.3 ml、一次注血组注入无抗凝自体血0.3 ml、二次注血组在1次注血后48 h再次注入0.3 ml.不同时间点在体观察基底动脉管径变化,动态检测大鼠顶叶皮层局部脑组织血流量(rCBF).结果 (1)注血1 h后,一次及二次注血组基底动脉痉挛明显,与对照组比较均有显著显著性差异(P<0.01),注血3 d后一次注血组基底动脉痉挛得到缓解,二次注血组基底动脉则持续痉挛,与一次注血组比较均有显著显著性差异(P<0.01).(2)注血1 h后.一次及二次注血组rCBF值均较对照组降低(P<0.01).注血3 d后,一次注血组rCBF逐渐恢复,二次注血组与一次注血组比较明显降低(P<0.01)结论 一次注血模型适宜研究早期脑血管痉挛,二次注血模型则可造成脑血管的持续性痉挛.

摘要： 目的:研究SD大鼠海马、额叶局部脑血流(rCBF)降低对学习记忆功能及γ-氨基丁酸(GABA)、乙酰胆碱酯酶(AchE)含量生化指标的影响及意义.方法:将健康成年SD大鼠随机分为手术组和假手术组.对2组大鼠进行学习记忆能力、右侧海马与额叶的rCBF、右侧海马与额叶的GABA、AchE含量的测定.结果:手术组大鼠的学习指标EN、达标所需日数、TRT(s)分别为52.09±8.43、0.56±0.12、14.42±0.51,明显大于假手术组(29.46±5.31、0.34±0.05、12.23±0.39),而主动回避率(%)63.57±6.13,明显低于假手术组(84.32±4.87),两组差异均具有显著性(P＜0.05).手术组大鼠右侧额叶、海马区术后4h、8h、24h、3d时的rCBF明显低于假手术组相应脑区同一时点的rCBF(P＜0.05).手术组大鼠手术后不同时间额叶、海马区AchE、GABA含量则较对照组显著升高(P＜0.05).结论:大鼠右侧额叶、右侧海马的rCBF rCBF的降低可能是导致其学习记忆能力障碍的重要危险因素;大鼠额叶、海马rCBF的降低所致该脑区GABA、AchE含量的变化,可能是大鼠学习记忆功能降低的生化基础.

摘要： Platelet Amyloid Precursor Protein ratio of different abnormal forms and 99mTc-ECD SPECT perfusion analysis were evaluated in Mild Cognitive Impairment (MCI) subjects who progressed to Alzheimer Disease (AD) and in stable MCI. We report that their combined evaluation increases the discriminative power of the analysis in identifying presymptomatic AD. The positive predictive value of these combined markers in identifying progressive MCI was 0.87, and the negative predictive value was 0.90. This observation suggests that the interplay of different markers should be considered for enhancing diagnostic accuracy of pre-clinical AD.

摘要： cqvip:In sensory substitution, information acquired with one sensory modality is use d to accomplish a task which is normally subserved primarily by another sensory modality. We used PET to study cross-modal plasticity in the congenitally blind , using electrotactile stimulation of the tongue. Blind (n=6) and sighted blindfolded controls (n=5) were scanned before and after they were trained to use their tongue in a Snellen orientation detect ion task. Results showed that both groups of subjects learned the discrimination orientation task after seven 1 h training sessions. Before training, no signifi cant changes in regional cerebral blood flow (rCBF) were observed in the occipit al cortex in either group. In sharp contrast, activity in the occipital cortex i ncreased after practice for the blind, but not for the sighted, providing eviden ce for training-induced plasticity in the blind. An inter-regional correlation analysis showed that task-related rCBF changes in left posterior parietal cort ex were positively correlated with rCBF changes in the occipital area of the tra ined blind. These data reveal that cross-modal plasticity in the blind develops rapidly and that the occipital cortex is part of a functional neural network fo r tactile discrimination in conjunction with the posterior parietal cortex. Our data further show that the tongue can act as a portal to convey somatosensory in formation to visual cortex.