心脏保存

摘要： 目的:低温冷浸泡心脏保存是临床上运用最广泛的供体心脏保存手段,但其安全时限仅4~6小时。Cirbp是一种冷休克蛋白,冷刺激时表达增多,作为RNA结合蛋白发挥作用,其在低温心肌保护机制中未被明确。本研究旨在通过蛋白质组学技术探究Cirbp在供体心脏低温冷保存过程的相关分子机制。

摘要： 目的 探索一种全新的器官保存方法,并通过检测实验指标探讨其对器官离体保存的作用与机制.方法 以BALB/c 7周雄性小鼠为供受体,将供体心脏取出后,离体保存24 h,通过颈部异位移植移植到受体BALB/c 7周雄性小鼠中.实验分为3个实验组,分别为一氧化碳(CO)高压保存24 h移植组(CO组);组氨酸-色氨酸-酮戊二酸盐液(HTK)浸泡保存24 h移植组(HTK组);即刻移植组(正常组).移植后记录心脏的复跳率;24h取异位心脏检测.检测心肌组织中血红素加氧酶-1(HO-1)及诱导型一氧化氮合酶(iNOs)含量,原位细胞凋亡检测(TUNEL)及羟基脱氧鸟苷(8-OHdG)检测.结果 Ho-1(ho-1/α-tubulin比值):CO保存组(0.245)表达明显高于HTK保存移植组(0.168)和即刻移植组(0.058,P＜0.05),而iNOs(即iNOS/α-tubulin值)的表达量最高是HTK保存移植组(0.268),其次是CO保存移植组(0.124)及即刻移植组(0.082).心肌凋亡百分比中,HTK保存移植组凋亡百分比明显高于CO保存移植、即刻移植两组,差异有统计学意义(P＜0.05).结论 外源性的CO保存心脏,能通过有效的抗凋亡和减轻氧化损伤,减轻炎性反应等作用而对离体心脏起保护作用,而这种保护作用可能是通过调高心肌组织中的HO-1含量、抑制iNOs产生以及CO本身联合作用而形成.%Objective To explore a new method of organ preservation,we assess the effect and mechanism of carbon monoxide (CO) on heart preservation.Methods BALB/c 7 week male mice were used to establish a model of mice cervical heterotopic heart transplantation.Mice were randomly divided into three groups:hearts preserved in CO gas combined with high pressure for 24 h group (CO group),hearts immersed in HTK (histidine-tryptophan-ketoglutarate solution) for 24 h group (HTK group),and immediate transplantation group (control group).In CO and HTK groups,hearts were transplanted after 24 h preservation.The re-beating time and state of the transplanted hearts were recorded.Heart tissues were collected after 24 h transplantation.Levels of Human heme oxygenase-1 (HO-1),inducible nitric oxide synthase (iNOs),and 8-Hydroxy-desoxyguanosine (8-OHdG) were measured.Cell apoptosis of transplanted hearts were assessed by tunel staining.Results The HO-1 expression levels in CO group (0.245) were significantly higher than HTK group (0.168,P ＜ 0.05) and control group (0.058,P ＜ 0.05).While the iNOs expression levels of HTK group (0.268) were higher than CO group (0.124) and control group (0.082).The apoptosis index of HTK group was significantly higher than CO group and control group (P ＜ 0.05).Conclusion Exogenous CO can inhibit apoptosis,oxidative damage,and inflammation of donor hearts probably via increasing HO-1 and reducing iNOs.

摘要： 目的 研究长时程心脏不停跳保存方法和技术.方法 选用成年广西巴马小型猪6头,麻醉成功后,取胸部正中切口,全身肝素化,不切开心包.先在头臂干插灌注管,后切断上腔静脉和左下肺静脉,用手协助排空左心室,用停跳液灌注心脏.摘取心脏后与灌注装置连接并用去白细胞和去血小板的稀释血液灌注心脏,使心脏恢复跳动(非工作心脏模型).结果 不停跳保存6例离体心脏8h全部成功,保存期间灌注压没有上升.结论 心脏空跳保存是可行的,保存的关键技术是减轻红细胞破坏、维持适宜的灌注压、左心室有效的减压和防止心脏水分丧失.

摘要： Objective To investigate the protective effect of desiccation using carbon monoxide on isolated mouse hearts, and to provide a new preservation method for heart transplantation .Methods The modified Heron's technique for heterotopic cervical cardiotransplantation was used .C57BL/6 male mice (n=100) were used in this research.The donor mice (n=60, 4-6 weeks age) were randomly divided into 3 groups, HTK group (n=20), CO group (n=20) and Na-ive group (n=20).The receptor mice (n=40, 8-10 weeks age) were randomly divided into 2 groups, HTK group and CO group.The donor hearts from HTK group were preserved in HTK cardioplegic solution at 4°C for 24 hours, and then transplanted to receptors .The donor hearts from CO group were hung in a high -pressure tank ( PO2 =3 200 hPa, PCO=800 hPa) at 4°Cfor 24 hours, and then transplanted to receptors .Meanwhile, the hearts from Naïve group received no preservation.The re beat number of the donor heart was tested .The HO-1, LDH and CPK in myocardial cell 24 hours after reperfusion of the donor heart were assessed .Results The re-beat heart counts in HTK and CO groups were 7 (35%) and 15 (75%), with significant difference between them (P<0.05).The HO-1 in CO group was significantly higher than that in Naive group and HTK group .The LDH and CPK in CO group were significantly lower than those in HTK group ( P<0.05 ) .Comparing with Naive group , pathological changes were obviously observed in CO and HTK groups, including edema and inflammatory cell invasion;however, the injury was more prominent in HTK group .Conclu-sion Comparing with the traditional HTK preservation , the method of preservation by desiccation of using carbon monox-ide has a better effect for mouse isolated hearts for a long time preservation .%目的：探讨一氧化碳（ CO）干燥环境对离体小鼠心脏的保护作用，为心脏移植提供一种新的保存方式。方法采用C57BL／6雄性健康小鼠，建立同系小鼠心脏颈部异位移植模型。4～6周龄C57BL／6小鼠60只作为供体，随机分为3组（n＝20），即空白对照组（不做心脏移植）、HTK浸泡供体组及CO干燥供体组；8～10周龄C57BL／6小鼠40只作为受体，随机分为两组（n＝20），分别为HTK浸泡受体组、CO干燥受体组。 HTK浸泡供体组及CO干燥供体组小鼠心脏分别用两种方法（ HTK浸泡保存和CO高压干燥保存）在4°C环境下保存24 h，通过建立同系小鼠颈部异位移植模型，监测模型复灌2 h后供心复跳率，并在24 h后检测供体心肌LDH、CPK、OH－1等酶学指标，观察心肌结构的改变。结果（1）复灌2 h后，CO干燥受体组复跳15只， HTK浸泡受体组复跳7只，CO干燥受体组复跳率明显高于HTK浸泡受体组（P＝0.011）。（2）复灌24 h后，CO干燥受体组LDH、CPK均明显低于HTK浸泡受体组（P＜0.05）；CO干燥受体组的LDH与空白对照组之间差异无统计学意义（P＝0.946），而CO干燥受体组CPK明显高于空白对照组（ P＜0.001）；HTK浸泡受体组的LDH和CPK均明显高于空白对照组；而CO干燥受体组HO－1明显高于HTK浸泡受体组（P＜0.05），而HTK浸泡受体组与空白对照组之间差异无统计学意义（P＝0.449）。（3）心肌结构上，CO干燥受体组和HTK浸泡受体组较空白对照组均有明显改变，两组均可见心肌水肿及炎细胞浸润，而CO干燥受体组与HTK浸泡受体组相比，病理损伤较轻。结论与传统HTK浸泡保存相比，CO高压干燥保存方式能够减少心肌损伤，减轻炎症反应，在供心较长时间保存方面更有优势。

摘要： Objective To investigate the protective effect of high-pressure carbon monoxide for preservation of ex vivo rabbit heart graft in comparison with the conventional HTK cardioplegic solution preservation. Methods Heart grafts isolated from 85 New Zealand rabbits were randomly divided into Naive group (n=5), HTK group (n=40) and CO group (n=40). The grafts underwent no preservation procedures in Naive group, preserved at 4 °C in HTK cardioplegic solution in HTK group, and preserved at 4°Cin a high-pressure tank (PO2:PCO=3200 hPa:800 hPa) in CO group with Krebs-Henseleit solution perfusion but without cardioplegic solution. After preservation for 2, 4, 6, 8, 10, 14, 18, and 24 h, 5 grafts from the two preservation groups were perfused for 30 min with a modified Langendorff apparatus and examined for left ventricular systolic pressure (LVSP), left ventricular diastolic pressure (LVDP), arrhythmia score (AS), myocardial ultrestructure, and cardiac enzyme profiles. Results After preservation for 6 to 24 h, the cardiac enzyme profiles and systolic and diastolic functions were significantly better in CO group than in HTK group, but these differences were not obvious between the two groups after graft preservation for 2 to 4 h. Significant changes in the myocardial ultrastructures occurred in the isolated hearts after a 24-h preservation in both CO and HTK groups, but the myocardial damages were milder in CO group. Conclusion Preservation using high-pressure carbon monoxide can better protect isolated rabbit heart graft than the conventional HTK preservation approach especially for prolonged graft preservation.%目的：观察一氧化碳（CO）高压干燥环境对离体兔心的保护效果，与传统HTK液低温浸泡保存比较，为心脏移植提供一种新的保存方式。方法将85只新西兰兔随机分为3组，分别为空白对照组（n=5），CO组（n=40）和HTK组（n=40）。空白对照组在取下心脏后直接进行酶学检测，而两个保存组中，每组兔心在不同保存方式下（高压罐中干燥保存及HTK液中浸泡保存）分别保存2、4、6、8、10、14、18和24 h，每个时间点各5只。通过建立改良Langendorff离体兔心灌注模型，监测模型保存前后心肌舒缩功能变化情况，心律失常评分，检测CK、CK-MB等酶学指标，观察心肌超微结构改变。结果恢复灌注后30 min，（1）CO干燥组的心肌舒缩功能、心功酶和心律失常评分改变在短期保存（2~4 h）内与HTK浸泡组无明显差别，而在中长期保存（6~24 h）上， CO干燥组各项指标均优于HTK浸泡组；（2）保存24 h后2组心肌超微结构均存在明显改变，但CO干燥组心肌细胞内结构损伤较HTK浸泡组轻。结论 CO高压干燥环境确实对离体兔心具有保护作用，而且在长时间保存方面，CO高压干燥保存比HTK浸泡保存更有优势。

摘要： Aim:To explore a new pig isolated heart preservation and heart function evaluation method .Methods:A total of 12 Swedish health home pigs were divided into two groups according to blood type , the blood donor group and isolated heart donor group, and 6 in each group.The cardiopulmonary bypass machine were used to perfuse isolated hearts at different perfusion flow(0.76,1.00,2.00,3.00, and 4.00 L/min) under normal temperature (37 °C).The myocardial oxygen con-sumption, heart rate, blood pressure and hemodynamic index of isolated hearts were monitored .Results:There were signifi-cant differences in myocardial oxygen consumption , heart rate, systolic blood pressure , average pressure , left ventricular sys-tolic blood pressure of isolated hearts with different perfusion flow (F=23.500,331.010,77.701,10.101 and 89.301,P<0. 001).The perfusion flow was higher , the indexes mentioned above were higher in isolated heart (P<0.05).There was no sta-tistically significant difference in myocardial oxygen uptake rate among the 5 groups(F=0.426,P=0.788).Conclusion:The method for pig isolated heart preservation and accurate evaluation of cardiac function is established .%目的：探索一种新型供体心脏体外保存及功能评价的方法。方法：将血型一致的12头瑞典健康家猪作为实验对象，随机分为心脏供体组和血液供体组两组，每组6头。用体外循环机以不同流量（0．76、1．00、2．00、3．00、4．00 L／min）在37°C条件下灌注离体心脏，监测动静脉血气和离体心脏血压、心率、心肌耗氧量及氧摄取率。结果：不同灌注流量下，离体心脏心肌耗氧量、心率、收缩压、平均压、左室收缩压差异均有统计学意义（ F＝23．500，331．010，77．701，10．101和89．301， P 均＜0．001）。灌注流量较高，离体心脏上述各指标也较高（ F ＝0．460， P＞0．05）。不同灌注流量组心肌氧摄取率差异无统计学意义（F＝0．426，P＝0．788）。结论：该模型可使离体心脏心功能保持良好并得到较好的保存。

摘要： 目的 探讨异氟烷预处理延迟保护作用对离体大鼠心脏的冷保存效果.方法 SD大鼠36只,根据是否腹腔注射异氟烷随机分为实验组与对照组,再根据冷保存时间不同,分为4 h、6 h、8 h组,每组6只.监测保存前后心功能恢复情况、冠脉流出液中肌酸激酶(CK)、乳酸脱氢酶(LDH)释放量变化.结果 复灌期间随着离体心脏保存时间的增长,心功能恢复率逐渐降低,其中8 h实验组与对照组比较各项心功能指标保存前后恢复率差异有统计学意义(P<0.05).复灌后随着离体心保存时间的延长,各组心肌酶 CK、LDH 均有不同程度升高,其中实验与对照组之间冷保存8 h 有统计学意义(P<0.05).结论 异氟烷预处理延迟保护作用对冷保存8 h离体鼠心具有保护作用,可以显著提高保存后心功能,减轻心肌细胞的损伤.

摘要： Objective To study the effects of donor heart preserved with continuous on-pump beating-heart warm-blood perfusion and University of Wisconsin (UW) solution on hemodynamics and cardiac function after transplantation in pigs.Methods Twenty-four Bama miniature pigs were randomly divided into two groups:In beating-heart group (n=12),donor hearts were stored by on-pump perfusion with warm blood;In cold UW storage group (n=12),donor hearts were stored in cold UW solution .There were six pigs for donors and six for recipients in each group .Donor hearts were stored for 8 hours after isolation,and then were implanted into recipient pigs by orthotopic heart transplantation . Automatically rebeating of implanted heart and the frequency of defibrillation in both groups were recorded two hours after the aortic cross-clamp released,left and right ventricular function were measured as well .The changes of hemodynamics were observed 30 minutes after cardiopulmonary bypass.Results After being implanted into recipient pigs,6 donor hearts recovered automatic-beating 3 minutes after the aortic cross-clamp released in the beating-heart group;All of 6 donor hearts did not recovered automatic-beating in the cold UW storage group,and the frequency of defibrillation accounted for 12;Significant difference was found in the automatically rebeating rate of implanted heart between two groups (P<0.05).Two hours after the aortic cross-clamp released,left and right ventricular systolic pressure in the beating-heart group were significantly better than those in the cold UW storage group (all P<0.05);Thirty minutes after cardiopulmonary bypass, central venous pressure (CVP) and pulmonary capillary wedge pressure (PCWP) in the beating-heart group were significantly lower than those in the cold UW storage group (all P<0.05);Cardiac output(CO) and cardiac index(CI) in the beating-heart group were significantly higher than those in the cold UW storage group (P<0.05),and the dosages of dopamine and isoprenaline in the beating-heart group decreased significantly (all P<0.05).Conclusion The heart preservation with continuous on-pump beating-heart warm-blood perfusion can make recipient pigs which receive orthotopic heart transplantation get better cardiac function and stable hemodynamics in the early stage .%　　目的评价猪供心在体外循环下不停跳温血连续灌注保存方法与单纯UW液冷保存方法的植入效果。方法选取广西巴马小型猪24头，随机分为两组，体外循环下不停跳温血连续灌注供心保存移植组（不停跳组，供、受体各6头）和单纯UW液冷保存移植组（冷保存组，供、受体各6头）。两组供心取出后，分别采用体外循环下不停跳温血连续灌注保存法及单纯UW液冷保存8 h，然后按标准原位心脏移植方法植入受体猪。记录两组供心植入主动脉开放2 h后心脏自动复跳、除颤情况，测量主动脉开放2 h末的左右心室功能，观察体外循环后30 min血流动力学参数的变化。结果供心植入受体后，主动脉开放3 min内，不停跳组6头心脏全部自动复跳；冷保存组6头均未能自动复跳，心脏除颤12次；两组心脏自动复跳率差异有统计学意义（P＜0．05）。主动脉开放2 h，左右心室收缩压不停跳组优于冷保存组（P均＜0．05）；体外循环后30 min，中心静脉压（CVP）、肺毛细血管楔压（PCWP）不停跳组低于冷保存组（P均＜0．05）；心输出量（CO）、心脏指数（CI）不停跳组高于冷保存组（P＜0．05），多巴胺用量、异丙肾上腺素用量不停跳组明显减少（P均＜0．05）。结论体外循环下不停跳温血连续灌注供心保存方法，使原位心脏移植后受体猪早期获得更良好的心功能及血流动力学稳定。

摘要： 离体心脏的保存可以为即将准备心脏移植的患者提供供心来源.改进供心保存技术不仅能延长供心的保存时间,扩大供心来源,还能提高移植手术成功率和远期生存率.而随着冷冻技术的出现,深低温离体心脏保存得以发展.但目前深低温保存技术还处于动物实验阶段,在某些方面仍存在不明和争议,尚需进一步研究.本文简介深低温下冷冻保存离体心脏的现状和方法,分析其与以往临床上心脏保存方法 的不同点及优缺点.

摘要： 目的:采用小鼠、大鼠Langendorff离体心脏灌注模型,筛选有效延长心脏存活期的中药.方法:在改良的Euro-Collins液(mEC液)中添加各中药注射液,与单纯mEC液作对照,观察保存小鼠心脏3h后的心功能情况及保存7.5h、9h后的心脏复活率;观察保存20h后大鼠离体心脏的心功能.结果:保存3h后GL、ASH、CTL组的心功能及保存7.5h后的心脏复活率均好于PNS、EBHM.保存小鼠心脏9h后心脏复活率的高低依次是CTL>GL>ASH;复跳需时以CTL最短(3.81±2.26).大鼠离体心脏保存后的心功能好坏依次为CTL>GL>AMB>ASH,结论:CTL延长心脏保存时间的作用显著,GL次之.

摘要： 本发明公开了一种心脏移植用保存装置及其使用方法，涉及心脏移植技术领域，包括保护箱，所述保护箱的顶部转动连接有盖板，所述保护箱的顶部且位于盖板的下方开设有保存腔，该心脏移植用保存装置，通过设置有气囊、连接件与移动柱，操作人员关闭盖板时，推动移动柱下移，使得齿条带动传动齿轮转动，从而通过传动机构带动活塞向连通管方向移动，使得活塞推动储存筒内部的气体通过连通管进入气囊内部，使得气囊膨胀，使得气囊推动连接件向保存箱方向移动，使得连接件推动压板将保存箱限位住，使得本装置既能够对保存箱进行限位，本装置受到撞击时，气囊对保存箱受到的力进行缓冲，从而不会使得保存箱内部的心脏组织受到损伤。

摘要： 本发明涉及一种中华鳖心脏细胞连续细胞系，该细胞系保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC No.10597；还涉及该细胞系的构建方法和超低温冷冻保存方法，其中构建方法包括以下步骤：(1)原代培养：取中华鳖心脏经前处理后剪成组织小块，胰蛋白酶室温消化后接种于培养瓶中，当细胞铺满瓶底至90％以上时，开始传代培养；(2)传代培养：采用胰蛋白酶消化法，吹打瓶底，使贴壁细胞脱落，进行传代。本发明中构建的中华鳖心脏细胞连续细胞系可在分子细胞水平上进行中华鳖以及其他龟鳖动物的病毒性疾病研究并进行相关治疗药物的研究，例如染色体分析、虹彩病毒研究等，并且也可以满足对中华鳖种质资源保存和理论研究与应用的需要。

摘要： 本发明涉及一种短须裂腹鱼心脏细胞系的构建和超低温冷冻保存的方法，属于淡水水生生物细胞培养和超低温冷冻保存技术领域。该方法是以短须裂腹鱼心脏组织为材料，采用组织块法和两酶消化法，在含有胎牛血清和细胞生长因子，pH值为7.0-7.2 的M199培养液中培养；采用胰蛋白酶消化法进行传代培养；具体包括制备细胞培养液、原代培养及传代培养步骤。本发明的有益效果在于：1、操作简便；2、降低了纯组织块法对心脏细胞的损伤；3、构建的短须裂腹鱼心脏细胞系形态为成纤维样，生长状态良好，能够连续传代并直接应用于生物学特性研究，满足了对短须裂腹鱼种质资源保存和理论研究及应用的需要；4、该构建方法也适用于其他鱼类构建心脏细胞系。

摘要： 本发明公开了一种心脏移植用保存装置及其使用方法，涉及心脏移植技术领域，包括保护箱，所述保护箱的顶部转动连接有盖板，所述保护箱的顶部且位于盖板的下方开设有保存腔，该心脏移植用保存装置，通过设置有气囊、连接件与移动柱，操作人员关闭盖板时，推动移动柱下移，使得齿条带动传动齿轮转动，从而通过传动机构带动活塞向连通管方向移动，使得活塞推动储存筒内部的气体通过连通管进入气囊内部，使得气囊膨胀，使得气囊推动连接件向保存箱方向移动，使得连接件推动压板将保存箱限位住，使得本装置既能够对保存箱进行限位，本装置受到撞击时，气囊对保存箱受到的力进行缓冲，从而不会使得保存箱内部的心脏组织受到损伤。

摘要： 本发明涉及一种干燥保存人工心脏瓣膜的方法，所述方法包括采用将三种包含有机溶剂和甘油的溶液与人工心脏瓣膜分先后顺序接触，并从溶液处理过的人工心脏瓣膜中去除部分所述溶液，经灭菌、冷冻后干燥保存。经上述方法处理后的人工心脏瓣膜尺寸与脱水前尺寸基本相同，完全消除了水的副作用，使干燥组织不易受微生物影响，从而使医疗人员可以尽可能接近即用的形式得到。

摘要： 本发明涉及一种中华鳖心脏细胞连续细胞系，该细胞系保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC？No.10597；还涉及该细胞系的构建方法和超低温冷冻保存方法，其中构建方法包括以下步骤：(1)原代培养：取中华鳖心脏经前处理后剪成组织小块，胰蛋白酶室温消化后接种于培养瓶中，当细胞铺满瓶底至90％以上时，开始传代培养；(2)传代培养：采用胰蛋白酶消化法，吹打瓶底，使贴壁细胞脱落，进行传代。本发明中构建的中华鳖心脏细胞连续细胞系可在分子细胞水平上进行中华鳖以及其他龟鳖动物的病毒性疾病研究并进行相关治疗药物的研究，例如染色体分析、虹彩病毒研究等，并且也可以满足对中华鳖种质资源保存和理论研究与应用的需要。

摘要： 本发明提供了一种用于离体心脏保存的机械灌注系统，所述机械灌注系统包括中央处理器模块，动力模块，心脏存储模块，液体循环模块，监测模块，所述液体循环模块包含心脏灌注液存储器，所述心脏灌注液存储器中存储有心脏灌注液，所述心脏灌注液含有伊桐苷，人工血液，α‑半乳糖苷酶和α‑N‑乙酰半乳糖苷酶。本发明用于心脏保存的机械灌注系统能极大的扩展心脏供体的适用范围，能够很好的解决心脏供体来源不足和离体心脏无法长时间保存的问题。

摘要： 本发明提供了一种用于离体心脏保存的常温灌注液，所述灌注液每升含有以下物质：伊桐苷4.5mg、羟基淀粉3g、三磷酸腺苷7.5g、全氟碳化物40g、卵磷脂4.5g，半乳糖苷酶1.25mg，乙酰半乳糖苷酶1mg，二氮嗪2.5mg、组氨酸550mg，色氨酸210mg，谷氨酰胺170mg，精氨酸625mg，青霉素3g，氯化钠15mmol，氯化钾10mmol，氯化镁5mmol，NaHCO

摘要： 本发明提供了一种用于离体心脏保存的机械灌注系统，所述机械灌注系统包括中央处理器模块，动力模块，心脏存储模块，液体循环模块，监测模块，所述液体循环模块包含心脏灌注液存储器，所述心脏灌注液存储器中存储有心脏灌注液，所述心脏灌注液含有伊桐苷，人工血液，α‑半乳糖苷酶和α‑N‑乙酰半乳糖苷酶。本发明用于心脏保存的机械灌注系统能极大的扩展心脏供体的适用范围，能够很好的解决心脏供体来源不足和离体心脏无法长时间保存的问题。

摘要： 本发明提供了一种用于离体心脏保存的常温灌注液，所述灌注液每升含有以下物质：伊桐苷4.5mg、羟基淀粉3g、三磷酸腺苷7.5g、全氟碳化物40g、卵磷脂4.5g，半乳糖苷酶1.25mg，乙酰半乳糖苷酶1mg，二氮嗪2.5mg、组氨酸550mg，色氨酸210mg，谷氨酰胺170mg，精氨酸625mg，青霉素3g，氯化钠15mmol，氯化钾10mmol，氯化镁5mmol，NaHCO