摘要：
In order to determine the influence of storage temperature,storage time and incubation temperature on hatchability of Dastarcus helophoroides eggs,the hatchability of the eggs was studied by cryopreservation at 5,7.5,10,12.5,15 °C and incubation at 20,26,32 °C.The results showed that the low temperature of 5 °C was not suitable for preserving the eggs of Dastarcus helophoroides,because it caused the hatching rate to decrease significantly (P ＜ 0.05).Other low temperatures of 7.5-15 °Ccould be used to preserve the eggs,preservation within 70 days would not reduce egg viability,and there was no obvious difference between the hatchability of these tests and the control (no hypothermia treatment).Storage time had no significant effect on egg hatching rate within 70 days(P ＞ 0.05).The incubation temperature also had significant influence on the hatching rate of the eggs,stored at the same low temperature,the hatching rate of the eggs under the condition of 20 °C was the highest,which showed significant difference from the hatchability under 26 °C and 32 °C (P ＜ 0.05).The period of hatching peak was the eighth day to the twelfth day under 20 °C,and that of 26 °C was the seventh day to the eleventh day,and that of 32 °C was the fifth day to the eighth day.The hatching time of eggs was highly significantly correlated with the hatching rate on the same day (P ＜ 0.01),and the hatchability in the morning was significantly higher than that in the noon and evening.In conclusion,the optimum storage temperature for the eggs of Dastarcus helophoroides was 10 °C,the optimum incubation temperature was 20 °C,and the storage time should be within 70 days.Under these conditions,the hatching period was the fifth day to the fourteenth day,and the peak hatching period was the eighth day to the twelfth day,that was to say,the egg card should be released to forest at the fifth to the eighth days after being taken out from low temperature.The egg cards should be released at an average temperature of 20 °C in the field,when the hatching rate and parasitism rate of eggs were the highest.%为了明确储藏温度、储藏时间及孵化温度对花斑花绒寄甲卵孵化率的影响,采用5、7.5、10、12.5、15°C低温储藏和20、26、32°C诱导孵化相结合方法对花斑花绒寄甲卵的孵化率进行了研究.结果显示:5°C低温不适合储藏花绒寄甲卵,其会致使孵化率显著降低(P＜0.05),其他低温(7.5～15 °C)都可以用于储藏花绒寄甲卵,储藏70 d内,不会降低卵活力,孵化率与对照(未低温处理)差异不大;储藏70 d内,储藏时间对卵的孵化率没有显著影响(P＞0.05);诱导孵化的温度对花绒寄甲卵的孵化率存在显著影响,同一低温储藏的卵,20 °C条件下的孵化率最高,与26°C、32°C下的差异达显著水平(P＜0.05);总体上,20°C条件下的孵化高峰期为8～12d,26°C的高峰期为7～11d,32°C的高峰期为5～8 d;同一天内卵的孵化率与孵化时间存在极显著相关性(P＜0.01),早上的孵化率极显著高于中午和傍晚.花斑花绒寄甲卵的最佳储藏温度为10°C,最佳孵化温度为20°C,储藏时间应在70 d内,在此条件下,孵化期为5～14d,孵化高峰期为8～12d,即卵卡应在取出后5～8d释放于林间.野外释放卵卡也应在平均气温达20 °C时释放,此时卵的孵化率及寄生率最高.
