Blood

摘要： Blood exosomes,which are extracellular vesicles secreted by living cells into the circulating blood,are regarded as a relatively noninvasive novel tool for monitoring brain physiology and disease states.An increasing number of blood cargo-loaded exosomes are emerging as potential biomarkers for preclinical and clinical Alzheimer's disease.Therefo re,we conducted a meta-analysis and systematic review of molecular biomarkers derived from blood exosomes to comprehensively analyze their diagnostic performance in preclinical Alzheimer's disease,mild cognitive impairment,and Alzheimer's disease.We performed a literature search in PubMed,Web of Science,Embase,and Cochrane Library from their inception to August 15,2020.The research subjects mainly included Alzheimer's disease,mild cognitive impairment,and preclinical Alzheimer's disease.We identified 34 observational studies,of which 15 were included in the quantitative analysis(Newcastle-Ottawa Scale score 5.87 points)and 19 were used in the qualitative analysis.The meta-analysis results showed that core biomarkers including Aβ_(1-42),P-T181-tau,P-S396-tau,and T-tau were increased in blood neuro nderived exosomes of preclinical Alzheimer's disease,mild cognitive impairment,and Alzheimer's disease patients.M olecules related to additional risk facto rs that are involved in neuroinflammation(C1q),metabolism disorder(P-S312-IRS-1),neurotrophic deficiency(HGF),vascular injury(VEGF-D),and autophagy-lysosomal system dysfunction(cathepsin D)were also increased.At the gene level,the differential expression of transc ription-related factors(REST)and microRNAs(miR-132)also affects RNA splicing,transport,and translation.These pathological changes contribute to neural loss and synaptic dysfunction.The data confirm that the above-mentioned core molecules and additional ris k-related factors in blood exosomes can serve as candidate biomarkers for preclinical and clinical Alzheimer's disease.These findings support further development of exosome biomarkers for a clinical blood test for Alzheimer's disease.This meta-analysis was registered at the International Prospective Register of Systematic Reviews(Registration No.CRD4200173498,28/04/2020).

摘要： Bloody Mahjong is a kind of mahjong.It is very popular in China in recent years.It not only has the characteristics of mahjong's conventional state space,huge hidden information,complicated rules,and large randomness of hand cards but also has special rules such as Change three,Hu must lack at least one suit,and Continue playing after Hu.These rules increase the difficulty of research.These special rules are used as the input of the deep learning DenseNet model.DenseNet is used to extract the Mahjong situation features.The learned features are used as the input of the classification algorithm XGBoost,and then the XGBoost algorithm is used to derive the card strategy.Experiments show that the fusion model of deep learning and XGBoost proposed in this paper has higher accuracy than the single model using only one of them in the case of highdimensional sparse features.In the case of fewer training rounds,accuracy of the model can still reach 83%.In the games against real people,it plays like human.

摘要： Neisseria meningitidis(N.meningtitidis)is a gram negative diplococcus.Neisseria meningitidis(meningococcus)was first identified in the cerebrospinal fluid of a patient with meningitis in 1887 by Weichselbaum,and the epidemic was traced back to 1805[1].The serogroups A,B,C,W,X,and Y were the most frequent causes of meningococcal diseased[1].N.meningitidis usually colonizes the human nasopharynx[2].The common clinical manifestations of invasive N.meningitidis infection are meningococcemia and acute meningitis[2].Some patients with meningococcal disease may present with pneumonia,arthritis,pericarditis,conjunctivitis,epiglottitis,sinusitis,otitis,urethritis,and proctitis[2].However,patients rarely present with acute peritonitis initially.

摘要： Objective: To explore the application of intelligent blood temperature and humidity monitoring system in cold chain management of blood station. Methods: Through the monitoring of fifty sets of cold-chain equipment in the central blood station in Hezhou for 6 months, the differences between the management of the automatic temperature and humidity monitoring system and the manual management were compared in terms of real-time recording, equipment alarm, data storage, historical data traceability and data analysis. Results: Temperature and humidity automatic monitoring system can automatically real-time acquisition, transmission, storage and alarm according to the required time interval;meanwhile, historical data can be quickly exported and traced, data and charts can be analyzed, and the alarm is real-time and effective. Conclusion: The system can effectively monitor the process of blood cold chain in blood stations and play a key role in ensuring blood quality. It can be popularized and used in blood stations.

摘要： An experiment was conducted to evaluate the performance and blood parameters of West African Dwarf (WAD) bucks fed graded levels of toasted baobab seed meal for 84 days. Baobab seeds were washed, sun-dried and toasted uniformly using a cast iron pan. The toasted baobab seeds were crushed with a roller mill grinding machine to make baobab seed meal (BSM). The BSM was included at 0%, 25%, 50% and 75% levels in diets 1, 2, 3 and 4 respectively to replace soybean. Each treatment was replicated four times in a completely randomized design. Uncoagulated blood samples were collected from WAD bucks at the end of the 84 days’ feeding trial and analysed for packed cell volume (PCV), haemoglobin concentrate (Hb), red blood cells (RBC) and white blood cells (WBC). The mean corpuscular haemoglobin volume (MCV), mean corpuscular haemoglobin (MCH), platelets, neutrophils, lymphocytes, monocytes, eosinophils, and basophils were calculated using PCV, RBC and Hb. The blood meant for serological analysis was centrifuged at 1000 G for 10 minutes, after which the serum was separated and used for determining serum total protein (Tp), albumin, serum glutamic oxaloacetic transaminase (SGOT) and serum glutamic pyruvic transaminase (SGPT). The inclusion of baobab seed meal up to 50% in the experimental diet did not have any negative effect on the performance of the goats and did not pose any health challenge to the animals during the study period.

摘要： SUMMARY A married mother in her 50s acutely developed vomiting,diarrhoea and severe epigastric pain 2 weeks following discharge from an acute psychiatric inpatient unit.She presented to the emergency department complaining of a 2-day history of the above symptoms.Blood tests revealed neutrophilia,grossly raised inflammatory markers and amylase levels triple the normal range.Based on radiological investigations,she was treated for necrotising pancreatitis that quickly escalated to multi-system organ failure and a lengthy intensive care unit admission.Common causes of pancreatitis,including cholelithiasis,alcohol and other drugs,were ruled out.Despite this,she suffered recurrent episodes of pancreatitis with significant morbidity.Olanzapine,started during her psychiatric admission,was determined to be the offending agent.Two years following the discontinuation of olanzapine,the patient has had no further episodes of acute pancreatitis.

摘要： BACKGROUND The association between blood levels of fructosamine(FMN)and recurrent coronavirus disease 2019(COVID-19)is currently unclear.AIM To investigate a prospective relationship between blood levels of FMN and severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)reinfection.METHODS A total of 146 Chinese hospitalized patients infected with SARS-CoV-2 were consecutively collectively recruited and followed from January 2020 to May 2021.Diagnosis of COVID-19 and SARS-CoV-2 reinfection was based on the diagnostic criteria and treatment protocol in China.The levels of FMN were determined in blood and divided into tertiles based on their distribution in the cohort of COVID-19 patients.Multivariate-adjusted hazard ratios(HRs)with 95%confidence intervals(CIs)were estimated for SARS-CoV-2 reinfection across the tertiles of FMN levels.A Cox regression model was used to generate the HR for SARS-CoV-2 reinfection in the participants in the top tertile of FMN levels compared with those at the bottom.Disease-free survival was used as the time variable,and relapse was used as the state variable,adjusted for age,gender,influencing factors such as diabetes mellitus,hypertension,and corticosteroid therapy,and clinical indexes such as acute liver failure,acute kidney failure,white blood cell(WBC)count,C-reactive protein,prognostic nutritional index(PNI),and blood lipids.Kaplan-Meier analysis with log-rank tests was used to compare the survival rate between patients with elevated FMN levels(FMN>1.93 mmol/L,the top tertile)and those with nonelevated levels.RESULTS Clinical data for the 146 patients with confirmed COVID-19[age 49(39-55)years;49%males]were analyzed.Eleven patients had SARS-CoV-2 reinfection.The SARS-CoV-2 reinfection rate in patients with elevated FMN levels was significantly higher than that in patients with nonelevated FMN(17%vs 3%;P=0.008)at the end of the 12-mo follow-up.After adjustments for gender,age,diabetes mellitus,hypertension,corticosteroid therapy,WBC count,PNI,indexes of liver and renal function,and blood lipids,patients with nonelevated FMN levels had a lower risk of SARS-CoV-2 reinfection than those with elevated FMN levels(HR=6.249,95%CI:1.377-28.351;P=0.018).Kaplan-Meier analysis showed that the cumulative survival rate of patients infected with SARSCoV-2 was higher in patients with nonelevated FMN levels than in those with elevated FMN levels(97%vs 83%;log rank P=0.002).CONCLUSION Elevated levels of FMN are independently associated with SARS-CoV-2 reinfection,which highlights that patients with elevated FMN should be cautiously monitored after hospital discharge.

摘要： BACKGROUND Gastric cancer is a common malignant tumor.Early detection and diagnosis are crucial for the prevention and treatment of gastric cancer.AIM To develop a blood index panel that may improve the diagnostic value for discriminating gastric cancer and gastric polyps.METHODS Thirteen tumor-related detection indices,38 clinical biochemical indices and 10 cytokine indices were examined in 139 gastric cancer patients and 40 gastric polyp patients to build the model.An additional 68 gastric cancer patients and 22 gastric polyp patients were enrolled for validation.After area under the curve evaluation and univariate and multivariate analyses.RESULTS Five tumor-related detection indices,12 clinical biochemical indices and 1 cytokine index showed significant differences between the gastric cancer and gastric polyp groups.Carbohydrate antigen(CA)724,phosphorus(P)and ischemia-modified albumin(IMA)were included in the blood index panel,and the area under the curve(AUC)of the index panel was 0.829(0.754,0.905).After validation,the AUC was 0.811(0.700,0.923).Compared to the conventional index CA724,the blood index panel showed significantly increased diagnostic value.CONCLUSION We developed an index model that included CA724,P and IMA to discriminate the gastric cancer and gastric polyp groups,which may be a potential diagnostic method for clinical practice.

摘要： Novel non-/minimally-invasive and effective approaches are urgently needed to supplement and improve current strategies for diagnosis and management of hepatocellular carcinoma(HCC).Overwhelming evidence from published studies on HCC has documented that multiple molecular biomarkers detected in body fluids and feces can be utilized in early-diagnosis,predicting responses to specific therapies,evaluating prognosis before or after therapy,as well as serving as novel therapeutic targets.Detection and analysis of proteins,metabolites,circulating nucleic acids,circulating tumor cells,and extracellular vesicles in body fluids(e.g.,blood and urine)and gut microbiota(e.g.,in feces)have excellent capabilities to improve different aspects of management of HCC.Numerous studies have been devoted in identifying more promising candidate biomarkers and therapeutic targets for diagnosis,treatment,and monitoring responses of HCC to conventional therapies,most of which may improve diagnosis and management of HCC in the future.This review aimed to summarize recent advances in utilizing these biomarkers in HCC and discuss their clinical significance.

摘要： Blood myeloid cells are known to be dysregulated in coronavirus disease 2019(COVID-19),caused by SARS-CoV-2.It is unknown whether the innate myeloid response differs with disease severity and whether markers of innate immunity discriminate high-risk patients.Thus,we performed high-dimensional flow cytometry and single-cell RNA sequencing of COVID-19 patient peripheral blood cells and detected disappearance of non-classical CD14LowCD16High monocytes,accumulation of HLA-DRLow classical monocytes(Human Leukocyte Antigen-DR isotype),and release of massive amounts of calprotectin(S100A8/S100A9)in severe cases.Immature CD10LowCD101-CXCR4+/-neutrophils with an immunosuppressive profile accumulated in the blood and lungs,suggesting emergency myelopoiesis.

摘要： 本发明公开了一种双通道荧光探针Blood‑CDs及其制备方法和应用，该双通道荧光探针Blood‑CDs由鸡血和二水合柠檬酸三钠的混合物经水热法制得，该双通道荧光探针Blood‑CDs在水介质中对Fe3+和Hg2+具有高度选择性和灵敏性。本发明制得的双通道荧光探针Blood‑CDs的荧光在水介质中可被Fe3+和Hg2+淬灭，并且具有较高的选择性和灵敏性；此外，F‑可选择性地恢复Fe3+淬灭的荧光，Al3+可选择恢复Hg2+淬灭的荧光。因此，该双通道荧光探针Blood‑CDs可用于区分Fe3+和Hg2+的检测，特别是应用于牲畜饲料或环境水样中Fe3+和Hg2+的定量检测和鉴别。