摘要：
Objective:To observe the effect of acupuncture on blood oxygen concentration in the brain of rats with post-traumatic stress disorder (PTSD) based on functional near-infrared spectroscopy (fNIRS),thus to reveal the mechanisms of acupuncture in intervening the brain function of PTSD rats.Methods:Sixty Sprague-Dawley (SD) rats were randomly divided into a blank group,a model group,a grasping group,a paroxetine group and an acupuncture group,with 12 rats in each group.Except the blank group,rats in the other groups all received incarceration plus electric shock for 7 d to prepare the PTSD animal model.One hour before the stress model was established,rats in each group received the designated intervention:rats in the blank group and the model group did not receive any intervention;rats in the grasping group received grasping and fixation;rats in the paroxetine group received paroxetine hydrochloride solution by intragastric administration;and rats in the acupuncture group received acupuncture.Six-day treatment was a course,with 2 courses of treatment conducted for a total of 12 d.After the modeling,rats in each treatment group received intervention for 5 d,and the fNIRS system was used to collect and record the changes in the concentrations of oxygenated hemoglobin (HbO2),deoxygenated hemoglobin (d-Hb) and total hemoglobin (t-Hb) of the involved rat's brain regions,and also to assess the brain function.Results:Compared with the blank group,the concentration of HbO2 was significantly increased,the concentration of d-Hb was significantly decreased,and the concentration of t-Hb was significantly increased in the model group and the grasping group after the intervention,and the differences were statistically significant (all P＜0.01).Compared with the model group,the concentrations of HbO2,d-Hb and t-Hb in the grasping group did not change significantly (all P＞0.05).Compared with the grasping group,the concentration of HbO2 was significantly decreased,the concentration of d-Hb was significantly increased,and the concentration of t-Hb was significantly decreased in the paroxetine group and the acupuncture group,and the differences were statistically significant (all P＜0.05).There were no significant differences in the concentrations of HbO2,d-Hb and t-Hb between the paroxetine group and the acupuncture group (all P＞0.05).Conclusion:Acupuncture can regulate the blood oxygen concentration in the brain of PTSD model rats,which may be an important mechanism of acupuncture in intervening the brain function in PTSD rats.%目的:基于功能近红外光谱技术(fNIRS)观察针刺对创伤后应激障碍(PTSD)模型大鼠脑部血氧浓度的影响,揭示针刺干预PTSD的脑功能机制.方法:将60只Sprague-Dawley (SD)大鼠随机分为空白组、模型组、抓取组、帕罗西汀组和针刺组,每组12只.除空白组外,其余各组大鼠均以电击幽闭法复制PTSD动物模型,模型复制共7d.于应激造模前1h,各组接受相应干预:空白组和模型组不接受任何干预,抓取组接受抓取固定,帕罗西汀组接受盐酸帕罗西汀溶液灌胃,针刺组接受针刺治疗,6d为1个疗程,连续干预2个疗程,共计12 d.造模结束后,各治疗组连续治疗5d后利用fNIRS系统采集并记录各组大鼠相关脑区组织氧合血红蛋白(HbO2)、脱氧血红蛋白(d-Hb)和总血红蛋白(t-Hb)浓度的变化情况,并进行脑功能评估.结果:干预结束后,与空白组比较,模型组和抓取组大鼠HbO2浓度显著升高,d-Hb浓度显著降低,t-Hb浓度显著升高,差异均具有统计学意义(均P＜0.01);与模型组比较,抓取组大鼠HbO2、d-Hb和t-Hb浓度均无明显变化(均P＞0.05);与抓取组比较,帕罗西汀组和针刺组大鼠HbO2浓度显著降低,d-Hb浓度显著升高,t-Hb浓度显著降低,差异均具有统计学意义(均P＜0.05);帕罗西汀组与针刺组的HbO2、d-Hb和t-Hb浓度均无统计学差异(均P＞0.05).结论:针刺对PTSD模型大鼠脑区血氧浓度有良性的调节作用,这可能是针刺干预PTSD的重要脑功能机制.
