摘要:
目的:观察不同灸量隔姜灸对脾虚证大鼠血清三叶因子1(TFF1)和粘蛋白5AC(MUC5AC)含量,以及胃黏膜表皮生长因子受体(EGFR)蛋白表达的影响,探讨隔姜灸治疗脾虚证的可能作用机制及量效特征.方法:将75只SPF级Sprague-Dawley(SD)大鼠按随机数字表法分为空白对照组(A组)、模型组(B组)、隔姜灸3壮组(C1组)、隔姜灸6壮组(C2组)和隔姜灸9壮组(C3组),每组15只.除A组外,其余各组大鼠采用200%的大黄浓缩液4°C灌胃制作脾虚证大鼠模型.造模成功后,B组大鼠不予治疗;C1、C2和C3组大鼠分别接受3壮、6壮和9壮隔姜灸足三里和中脘治疗,连续治疗8 d.观察大鼠一般症状评分,采用酶联免疫吸附法(ELISA)检测血清中TFF1和MUC5AC含量;免疫组化法检测胃黏膜EGFR蛋白表达.结果:干预结束后,与A组比较,B组大鼠脾虚症状积分增高,C1、C2和C3组大鼠血清TFF1、MUC5AC含量及胃组织EGFR蛋白表达明显升高(均P0.05).结论:隔姜灸能改善大鼠脾虚症状,促进脾虚证大鼠胃黏膜的增殖修复,其作用机制可能与提高血清TFF1和MUC5AC含量,激活EGFR蛋白的表达相关,且灸9壮和6壮的疗效优于灸3壮,但灸9壮和灸6壮的效果相当.%Objective: To observe the effects of different doses of ginger-partitioned moxibustion on serum trefoil factor 1 (TFF1) and mucin 5AC (MUC5AC) levels, as well as the expression of epidermal growth factor receptor (EGFR) in gastric mucosa of rats with spleen deficiency syndrome, therefore, to explore the possible mechanism and the dose-effect characteristics of ginger-partitioned moxibustion in spleen deficiency syndrome. Methods: Seventy-five SPF grade Sprague-Dawley (SD) rats were randomly divided into a blank control group (group A), a model group (group B), a 3 moxa-cone ginger-partitioned moxibustion group (group C1), a 6 moxa-cone ginger-partitioned moxibustion group (group C2) and a 9 moxa-cone ginger-partitioned moxibustion group (group C3) using random number table method, 15 rats in each group. Except group A, rats in the other groups received intragastric administration of 4 °C200% concentrated Da Huang (Radix et Rhizoma Rhei) to prepare spleen deficiency syndrome model. After successful modeling, rats in group B received no treatment; rats in group C1, C2 and C3 were treated with 3, 6 and 9 moxa-cone ginger-partitioned moxibustion at Zusanli (ST 36) and Zhongwan (CV 12) respectively for 8 continuous days. The general symptom score of rats was observed. The serum levels of TFF1 and MUC5AC were detected by enzyme-linked immunosorbent assay (ELISA). The expression of EGFR protein in gastric mucosa was detected by immunohistochemistry. Results: After the treatment, compared with group A, the spleen deficiency symptom score was increased in group B, the levels of serum TFF1 and MUC5AC, the EGFR protein expression in gastric tissues of group C1, C2 and C3 were significantly increased (all P0.05). The mechanism may be related to the increase of serum TFF1 and MUC5AC levels and activation of EGFR protein. Conclusion: Ginger-partitioned moxibustion can improve the symptoms, as well as promote the proliferation and repair of gastric mucosa in rats with spleen deficiency. The therapeutic efficacy of 6 or 9 moxa-cone ginger-partitioned moxibustion is better than that of 3 moxa-cone ginger-partitioned moxibustion, while the efficacies are equivalent between 6 and 9 moxa-cone ginger-partitioned moxibustion groups.