摘要：AIM：To discuss the expression ofglactin-3 in liver metastasis of colon cancer and its inhibition by modified citrus pectin(MCP)in mice．rn METHODS：Seventy-five Balblc mice were randomly divided into negative control group(n=15)，positive control group(n=15)，low MC concentration group(n=15)，middle MCP concentration group(n=15)and high MCP concentration group(n=15)．CT26 colon cancer cells were injected into the subcapsule of mouse spleen in positive control group，low，middle and high MCP concentrations groups，except in negative control to set up acolon cancer liver metastasis model．The concentration of MCP indrinking water was 0．0％，0．0％。1．0％．2．5％ and 5．0％(wt/vo1)，respectively．Liver metastasis of colon cancer was observed after 3 wk．Enzyme-linked immunosorbent assay(ELISA) was used to detect the concentration of galectin-3 in scmnl．Expression of galectin-3 in liver metastasis was detected by immunohistochemistry．rn RESULTS：Except for the negative group。the percentage of liver metastasis in the other 4 groups Was 100％，80％，73.3％and 60％,respectively．The number of fiver metastases in high MCP concentration group Was significantly less than that in positive centrol group (P=0．008)．Except for the negative group，the median volume of implanted spleen tumor in the other 4 groups Was 1．51cm3，0．93cm3，0．77cm3 and 0．70cm3，respectively．The volume of implanted tumor in middle and high MCP concentration MCP groups Was significantly smaller than that inpositive controlgroup(P=0．019；P=0．003)．The concentration of serumgalectin-3 in positive control and MCP treatment groups Was significantly higher than that in the negative group．However,there Was no significant difference between them．Except for the negative group,the expression of galectin-3 in liver metastases of the other 4 groups showed no significant difference．rn CONCLUSIONS：Expression of galetin-3 increases significantly in liver metastasis of colon cancer。which Can be effectively inhibited by MCP．
