从泡菜中筛选出对铅具有抗性和吸附性的乳酸菌,用于重金属铅的去除.用涂布平板法从泡菜中共分离筛选到4株对铅的抗性达2000mg/L的菌株,其中在含铅100 mg/L时p3菌株对铅的吸附率最高,达到78.90％.通过形态观察、生理生化鉴定、质谱鉴定、16S rDNA序列分析等方法,证明所得p3菌株为短乳杆菌(Lactobacillus brevis).该菌株在含盐0～5％时生长良好,能耐受pH为2的酸、8.0 g/L胆盐和1.1 g/L的胰蛋白酶.将来可于废水处理、制作泡菜或作为益生菌用于动物体内,减少其体内铅含量.%Lead-resistant and adsorptivity lactic acid bacteria were screened out from pickles and used for the removal of heavy metal lead.Four strains were isolated from the samples by spread plate method,and lead resistant reached 2000 mg/L,when the concentration of lead was 100 mg/L,,the adsorption rate of p3 was highest,reached 78.90％.The results of morphology observation,physiological and biochemical identification and mass spectrometry detection and 16S ribosomal deoxyribonucleic acid sequencing data showed that p3 could be identified as Lactobacillus brevis.The strain grew well in the salt containing 0～5％,and it was able to tolerate the hydrochloric acid with pH value of 2,the salts of 8.0 g/L and trypsin of 1.1 g/L.It can be used to treat waste water,make pickles orreduce lead levels in animals as a probiotic in the future.
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