首页> 中文期刊> 《特产研究》 >杜马斯燃烧法和凯氏定氮法在人参蛋白质含量检测中的对比研究

杜马斯燃烧法和凯氏定氮法在人参蛋白质含量检测中的对比研究

         

摘要

The paper selected 14 ginseng samples and 2 standard reference materials as the measure and study objects ,determined and analyzed the protein content of these tested samples by Kjeldahl and combustion methods ,respectively.The results showed that no significant differences were found in the quantitative results( P>0.05).With the Kjeldahl determination ,the average coefficient of variation (CV) was 2.010% and the CV was 1.156% which produced with the combustion method ,less than 5%.The protein content of most testing samples measured with the combustion method was higher than those with the Kjeldahl method.The average ratio of measured results which obtained by above two methods was 1.023.It was concluded that combustion method can be used as a regular method for determination of protein contents in ginseng.%选取14个人参样品和2种化学标准物作为试验材料 ,分别采用杜马斯燃烧法和凯氏定氮法测定16个试验样品中的蛋白质含量.结果显示 ,2种测定方法测得的人参中蛋白质含量结果差异不显著( P>0.05 ) ,凯氏定氮法的测定值平均变异系数CV=2.010% ,杜马斯燃烧法测定值平均变异系数CV=1.156% ,2组变异系数均小于5% .大部分样品的杜马斯燃烧法测定值高于凯氏定氮法测定值.2种方法测定值比值的平均值C/K=1.023.综上 ,杜马斯燃烧法可以作为人参蛋白质含量的常规检测方法.

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