大孔树脂吸附分离米糠中ACE抑制肽工艺

摘要

To screen the isolation and purification of rice bran protein,macroporous adsorption resin was used to separate rice bran ACE inhibition peptide,and compared the effect of adsorption and desorption of rice bran protein peptide in the 6 kinds of macroporous adsorption resin. The results showed that the HPD-400 type resin was the most suitable in the rice bran protein peptide. Process parameters were confirmed by systematacially studying the influence of various factors on the resin adsorption and desorption.The time for static adsorption equilibrium of HPD-400 resin to rice bran polypeptide was 4 h,appropriate adsorption temperature was about 45℃,after adsorption time was over 2 h,adsorption capacity of HPD-400 resin to rice bran protein peptide had already reached saturation,when pH was 4,adsorption effect was better,and the adsorption rate was 85.4%. Desorption solution was 70% ethanol solution and pH was 8. The elution time was determined as 30 min. The experiments of dynamic adsorption and separation showed that the resin could achieve the purpose of purification of ACE inhibitory peptides from rice bran.%采用大孔吸附树脂对米糠ACE抑制肽进行分离，比较6种大孔吸附树脂对米糠蛋白中肽的静态吸附和解吸效果，从中筛选出适合该米糠蛋白中肽分离纯化的树脂。结果表明，筛选出HPD-400型树脂最适合米糠蛋白中肽的混合物的纯化。通过对影响树脂吸附解吸的各种因素进行系统地研究，确定工艺参数。HPD-400树脂对米糠多肽的静态吸附4 h左右基本达到吸附平衡，选择吸附温度45℃较为适宜；吸附时间达到2 h后HPD-400型树脂对米糠蛋白肽的吸附量已达到饱和；在pH 4.0时吸附效果好，吸附率达85.4%。解吸液为pH 8.0的70%乙醇溶液，洗脱时间确定为30 min。通过动态吸附分离实验得出，该树脂可以达到分离纯化米糠ACE抑制肽的目的。