紫玉米苞叶花色苷与牛血清白蛋白相互作用研究

摘要

Fluorescence spectrometry, UV-VIS absorption spectroscopy, and high-performance liquid chro-matography (HPLC) were used to research the interaction between purple corn bract anthocyanins and bovine serum albumin (BSA). The results showed that there was a relationship on the purple corn anthocyanins bract and BSA. The bonding degree increased with the increase of the concentration of anthocyanins . Furthermore, the structure of BSA was changed after the combination. The binding abilities of six anthocyanins in purple corn bract was different. The order of combining ability was as follows:pelargonidin-3-(6'-malonylglucoside)>pe-onidin-3-(6'-malonylglucoside)>pelargonidin-3-glucoside>cyanidin-3-glucoside>cyanidin-3-(6'-malonyl-glucoside)>peonidin-3-glucoside.%采用荧光光谱法、紫外可见吸收光谱法、高效液相色谱法研究紫玉米苞叶花色苷与牛血清白蛋白(BSA)之间的相互作用.结果表明,紫玉米苞叶花色苷与BSA之间存在一定的相互作用,结合程度随着紫玉米苞叶花色苷浓度的增加而增加,二者结合后,会引起BSA结构的变化.紫玉米苞叶中6种主要的花色苷成分与BSA结合程度不同,其结合能力大小依次为:天竺葵-3-(6'-丙二酰葡萄糖苷)>芍药-3-(6'-丙二酰葡萄糖苷)>天竺葵-3-葡萄糖苷>矢车菊-3-葡萄糖苷>矢车菊-3-(6'-丙二酰葡萄糖苷)>芍药-3-葡萄糖苷.