Liquid Chromatographic Studies of the Effect of Temperature on the ChiralRecognition of Tryptophan by Silica-Immobilized Bovine Albumin

摘要

The practical application of immobilized bovine serum albumin (BSA) as achromatographic packing for the resolution of enantiomers has been demonstrated by a number of investigators. In most cases minor changes in eluent conditions lead to very large variations in chromatographic resolution. Because of this, a better understanding of basic separation mechanisms and the influence of solution equilibria and other pertubating conditions such as temperature are necessary in order to assure chromatographic reproducibility as well as to design novel applications. The current work examines the effect of temperature and eluent pH on the chromatographic performance and binding characteristics of silica-immobilized BSA. Measurements of both the background and the site-specific