摘要:Considering Lmbr1 is a key candidate gene of chicken polydactyly, the study on search SNPs which was influence the polydactyl. By the methods of PCR-SSCP and direct sequencing, two synonymous mutation (c. 1326A> G, c. 1360C> A) and five genotypes (AABB,aaBB,AABb, AaBb, AaBB) in exon 16 of Lmbr1 gene were detected from Beijing-fatty chicken(4- teod and 5-teod). The statistical analysis showed that the a allele gene frequencies was advantage on c. 1326A>C locus of Lmbrl between double 5-teod and mingle-teod chicken group (0.83, 0.81),A allele gene frequencies was advantage in double 4 -teod chicken(0.53). aa genoptype was preponderance in 5-teod and mingle-teod chicken group (0. 650, 0. 625) ,Aa genotype was dominant position in double 4-teod chicken(0.42), AA was only found on 4-teod chicken group,and was not found in 5-teod and mingle-teod chicken group. B allele gene frequencies was advantage on c. 1326A>C locus in double 5-teod, mingle-teod and double 4-toed chicken group(0.85, 0.88, 0. 76), BB genoptype was preponderance in double 5-teod, mingle-teod and double 4-toed chicken group (0. 692, 0. 750, 0. 516), and was not found bb genoptype. In the chicken group, by Hardy-Weinberg equilibrium tested, c. 1326A>C locus was in non-equilibrium, c. 1360C> A locus was in equilibrium. The research showed that c. 1326A>G was close associate with polydactyly,the site may utilize to MAS.%将Lmbr1基因作为鸡多趾研究的候选基因,寻找与多趾有关的SNPs并进行相关性分析研究.通过PCR-SSCP及直接测序,在北京油鸡(四趾/五趾)Lmbr1基因第16外显子检测到2个位点存在同义突变(c.1326A>(G和c.1360C>A),5种基因型(AABB、aaBB、AABb、AaBb、AaBB).对c.1326A>G、c.1360C>A两位点在不司趾型的基因频率及基因型进行统计分析,结果发现,在c.1326A>G位点,双五趾和混合趾中a为优势等位基因(0.83、0.81),双四趾中A为优势等位基因(0.53),双五趾和混合趾中aa为优势基因型(0.650、0.625),双四趾中Aa为优势基因型(0.420),AA基因型仅出现在双四趾中,在双五趾和混合趾中没有发现.在c.1360C>A位点,双五趾、混合趾和双四趾中B均为优势等位基因(0.85、0.88、0.76);双五趾、混合趾和双四趾中BB均为优势基因型(0.692、0.750、0.516),在试验鸡群中没有发现bb基因型.在北京油鸡群体中,c.1326A>G等位基因处于Hardy-Weinberg非平衡状态(P<0.01),c.1360C>A等位基因处于Hardy-Weinberg 平衡状态(P>0.05).研究结果表明,c.1326A>G位点与鸡趾型密切相关,有望用于标记辅助选择.