基于单克隆抗体的食品中乳酸链球菌素酶联免疫方法

摘要

Anenzyme linked immunosorbent assay (ELISA) for quantitative detection of nisin was established.Nisin A coupled with bovine serum albumin (BSA) was used as antigen to prepare monoclonal antibody.The antibody could combine with Nisin A and Nisin Z equally and did not combine with other polypeptide antibiotics.The assay exhibited dynamic linear range for detection of Nisin from 0.05 μg/mL to 5.4 μg/mL (y =-0.12ln (x) + 0.385,R2=0.98),IC50was 0.49 ng/mL.The assay method could be applied in heat-processed products.The detection time for each sample was 60 min.%建立了乳酸链球菌素的酶联免疫检测方法.以乳酸链球菌素A偶联牛血清白蛋白作为抗原制备了单克隆抗体,该抗体能等同识别乳酸链球菌素A和Z,对其他多肽类抗生素无交叉反应.所建立的乳酸链球菌素检测方法IC50为0.49μg/mL,线性范围为0.05μg/mL ～ 5.4 tg/mL(y=-0.12ln (x)+0.385,R2=0.98).该方法适用于热加工样品的检测,前处理简单,整个检测过程仅需60min,为乳酸链球菌素速测提供了有力的技术手段.