常压室温等离子体诱变选育高产曲酸米曲霉

摘要

以米曲霉 KA2308作为出发菌株，首次利用常压室温等离子体诱变技术选育高产曲酸菌株，通过96孔板高通量筛选获得一株曲酸生产菌 KA1515，曲酸产量提高到47.28 g·L－1，比出发菌株提高了31.48％，经过多次传代，曲酸产量仍比较稳定。表明采用常压室温等离子体诱变能有效提高米曲霉曲酸产量。%The strain of Aspergillus oryzae KA2308 was exposed on atmospheric and room temperature plasma(ARTP)jet to improve kojic acid production for the first time.A high kojic acid-producing strain KA1515 was obtained by high-throughput screening method with 96-well plates.The yield of kojic acid of mu-tant reached up to 47.28 g·L-1 ,increasing by 31.48% compared with original strain.Kojic acid yield was sta-ble after several passages.The results showed that mutation by atmospheric and room temperature plasma could raise kojic acid yield of Aspergillus oryzae effectively.