免疫调节细胞FoxP3+Treg及免疫蛋白PD-L1在宫颈病变微环境中的表达

摘要

目的 检测免疫抑制细胞FoxP3+ Treg和免疫蛋白PD-L1在宫颈微环境中的表达情况.方法 采用免疫组织化学法检测FoxP3和PD-L1在20例正常宫颈组织及45例不同程度宫颈病变组织中的表达情况.结果 宫颈病变组织中的FoxP3+ Treg(H=43.211,P=0.000)和PD-L1蛋白(t=213.00,P=0.001)表达均明显高于正常组织;随着病理级别升高,FoxP3+ Treg的侵袭性(H=28.307,P=0.000)和PD-L1蛋白在异常分化细胞中的表达增加(t=239.000,P=0.028);FoxP3+ Treg与异常分化细胞中PD-L1的表达呈显著正相关(rs=0.364,P=0.003).结论 在宫颈癌进展的不同病理阶段,局部微环境中FoxP3+Treg数目随官颈细胞恶性转化的进程而增多,异常分化细胞中PD-L1的表达增强.%Objective To explore the expression patterns of immune negative regulator FoxP3 + Treg and PD-L1 protein in cervical carcinoma and its precancerous lesions.Methods The expression patterns of FoxP3 + Treg and PD-L1 protein in 45 cases of cervical lesions tissues of the cervix and 20 cases of normal cervix tissues by using immunohistochemistry (IHC).Results Compared with the normal cervix, the expressions of FoxP3 +Treg (H =43.211, P =0.000) and PD-L1 protein (t =213.00, P =0.001) were significantly increased in cervical lesions.Compared with the low-grade squamous cell carcinoma, the invasiveness of FoxP3 + Treg was increased in high-grade squamous cell carcinoma (H =28.307, P =0.000), along with increased expression of PD-L1 protein (t =239.000, P =0.028).The FoxP3 + Treg expression was positively correlated with PD-L1 protein expression in abnormally differentiated cells (rs =0.364, P =0.003).Conclusion Along with the progression of cervical cancer, the FoxP3 + Treg amount increases in the local microenvironment, along with enhanced PD-L1 protein expression in abnormally differentiated cells.