Detection of Incipient Germination in Malting Barley with a Starch Viscosity Method and a Proposed Ethanol Emission Method

摘要

The risk of germination loss during storage in two-row malting barley can be reduced by identifying grain lots that have undergone incipient germination (IG) during harvest. A method based on starch viscosity that utilizes a Rapid Visco Analyzer (RVA) is currently available for IG analysis. A new potential method, based on the measurement of ethanol emission (EE) from whole barley, may be more efficient and less expensive than the RVA procedure. Three storage experiments were performed: experiments 1 and 3 at 25 degrees C and 80% rh, and experiment 2 in unheated, uninsulated buildings. Decline in germination energy (GE) varied from 1% to about 80% during storage. In experiments 1 and 3, R-2 for GE loss (expressed as [weeks to 5% loss of GE](0.2)) versus EE was 0.76 in both cases, whereas R-2 for GE loss versus RVA viscosity was 0.64 and 0.68, respectively. In experiment 2, the greatest loss of GE was associated with high temperatures and relative humidity in July and August. EE performed as well as RVA in the prediction of germination loss in all three trials. Although further evaluation of the EE method is required, it appears to be a promising alternative to the RVA technique.