Optimization of enzymatic hydrolysis with Cryotin F on antioxidative activities for shrimp hydrolysate using response surface methodology.

摘要

Cryotin F may be suitable for hydrolysing shrimp wastes (shells and heads) into bioactive ingredients which could be used as value-added products. Optimum conditions for antioxidative activities of the enzymic hydrolysate produced using Cryotin F were determined using response surface methodology with central composite rotatable design. Experimental ranges of independent variables for 20 experimental runs were, rection temp. = 28.2-61.8 degrees C, pH = 6-10 and enzyme concn. = 0.5-5.5%. Degree of hydrolysis for reaction products was measured and antioxidative activities were determined using 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging and Fe-chelating activities. Changes in enzyme concn. did not affect antioxidative activities significantly (P < 0.05). DPPH scavenging activity and chelating activity against Fe for enzyme hydrolysates were affected more by pH of enzyme hydrolysis than action temp. DPPH-scavenging activity was higher at acidic than at alkali pH while chelating activity was inversely affected. Hydrolysates of shrimp wastes showed high antioxidative activities depending on treatment conditions.