Caracteriza??o parcial química e funcional de produtos de soro de leite obtidos por diferentes processos

摘要

Whey protein samples (S-1 to S-5) were tested invivo and invitro for nutritional properties and selected bioactivities. Weanling male Wistar rats fed modified AIN-93G (12gprotein.100g-1) diets for 21 days were used the invivo studies. The nutritional parameters did not differ among the protein diets tested. Erythrocyte glutathione content was considered high and was higher for S-3, but liver glutathione was the same for all dietary groups. For S-3, cytokine secretion (IL-10 and TNF-α) by human peripheral blood mononuclear cells (in RPMI-1640 medium) was higher in the absence of antigen than in the presence of BCG antigen. Interleukin-4 secretion was repressed in all treatments. The IC50, whey protein concentration required to inhibit 50% of the melanoma cell proliferation, was 2.68mg.mL-1 of culture medium for the S-3 sample and 3.66mg.mL-1 for the S-2 sample. Based on these results, it was concluded that S-3 (whey protein concentrate enriched with TGF-β and lactoferrin) produced better nutritional and immunological responses than the other products tested.