米糠中γ-氨基丁酸的富集及纯化工艺研究

摘要

The accumulation of GABA in rice bran by glutamate decarboxylase(GAD) ,and the purification of GABA enriched solution using cation ion exchange resin were investigated. The optimal accumulation conditions of GABA were determined as described below: the ionic strength of citric acid-disodium hydrogen phosphate buffer solution was 0.02mol/L, the reaction time was 16h. Under the optimal conditions, the concentration of GABA reached 2900mg/100g rice bran. The optimal purification conditions of GABA enriched solution also be determined as following: the optimal pH of sample solution was 2.0, the concentration of GABA enriched solution was 2.0mg/mL,concentration of eluting agent was 2.0mol/L. And the final purity of GABA reached 61.25％.%以米糠为原料,利用米糠中高活性谷氨酸脱羧酶(GAD)进行γ-氨基丁酸(GABA)的富集实验,并采用阳离子交换树脂对富集液中GABA进行分离纯化.结果表明:采用0.02mol/L Ph5.6的Na2HPO4-柠檬酸缓冲液进行GABA富集实验,反应16h后可得到GABA 2900mg/100g米糠.采用D001大孔强酸性阳离子交换树脂对该富集液进行纯化实验,调节富集液Ph2.0,以2mg/Ml的浓度上样吸附,2mol/L的氨水浓度进行洗脱,最终可得γ-氨基丁酸纯度61.25%.