Microorganisms are prone to form bacterial biofilm on the equipment surfaces in chilled pork,which causes meat spoilage,foodborne diseases prevalence and food poisoning.This article investigated the formation law of microflora from chilled pork on the surfaces of glass and stainless steel at different temperatures,and isolated and identified biofilm-forming bacteria,and removed biofilm with sodium hypochlorite and ultrasonic method.The results showed that bacteria biofilm reached the maximum at 10,6 and 3 d at 4,15 and 26 ℃,respectively,and the amount of biofilm formed on stainless steel was greater than that of glass.A total of 9 biofilm-forming strains were isolated and identified,including Proteus vulgaris,Streptococcus,Pseudomonas and so on.By sodium hypochlorite soaking,the removal effects on Proteus biofilm on stainless steel and glass surfaces were significantly(p ＜ 0.05).The removal effects of 200,400 and 600 mg/L sodium hypochlorite solution soaking for 5 min could reach 98％.100 kHz ultrasound 1 min combined with 400 mg/L NaClO solution soaked in 5 min cleanup effect was up to 99％,that could be better than 400 mg/L NaClO solution and ultrasonic alone.%冷鲜肉中微生物极易在设备表面附着形成生物膜,引起肉类腐败变质,同时还会引起食源性疾病传播和食物中毒.本文研究不同温度下冷鲜猪肉中菌群在玻璃和不锈钢表面的成膜规律,分离鉴定成膜微生物,并采用NaClO、超声波清除生物膜.结果表明,4、15和26℃生物膜形成量分别在第10、6和3d达到峰值,且细菌在不锈钢表面形成的生物膜量大于玻璃材质;分离鉴定出普通变形杆菌、腐败希瓦氏菌和假单胞菌等9株成膜细菌. NaClO溶液浸泡对不锈钢和玻璃表面的变形杆菌生物膜均有显著清除效果(p＜0.05).采用200、400和600 mg/L NaClO溶液浸泡5 min其清除效果均能达到98％以上.100 kHz超声1 min结合400 mg/L NaClO溶液浸泡5 min清除率达99％以上,效果优于NaClO溶液和超声波单独作用.
展开▼
