采用响应面法优化蛋白核小球藻（Chlorella pyrenoidesa）叶绿素提取工艺，研究影响其稳定性的相关因素。结果表明，小球藻叶绿素最优提取条件为：固液比5 g·L-1，超声温度62℃，超声时间2 h；在此条件下，模型预测提取量为11.24 mg·g-1，验证试验提取量为10.99 mg·g-1。室内自然光、人工光、UV辐射、高温、酸、碱、柠檬酸、高浓度麦芽糖、金属离子Al3+、Fe2+和Fe3+等对小球藻叶绿素稳定性有不同程度破坏作用；而氧化还原剂、葡萄糖、蔗糖、低浓度麦芽糖、VC、食盐、金属离子Mg2+、Ca2+、K+、Zn2+和Na+等对叶绿素稳定性影响不显著；Cu2+可增强小球藻叶绿素稳定性。%Response surface methodology (RSM) was employed to optimize the extraction conditions of chlorophyl from Chlorel a pyrenoidesa. And effects of factors on stability of chlorophyl were also studied. Results showed that the optimum extraction conditions were solid-to-solvent ratio 5 g·L-1, ultrasonic tempera-ture 62 ℃, and ultrasonic time 2 h. Under these conditions, the maximum predicted extraction amount of chlorophyl from C. pyrenoidesa was 11.24 mg·g-1, which was close to the actual value of 10.99 mg·g-1. In ad-dition, it was showed that these factors, including natural light in room (NL), artificial light (AL), ultraviolet radi-ation (UV), high temperature, strong acid/base, citric acid, high concentration of maltose, Al3+, Fe2+and Fe3+, had remarkable effects on the stability of chlorophyl . However, other factors, such as oxidant and reductant, glucose, sucrose and low concentration of maltose, vitamin C, salt, and Mg2+, Ca2+, K+, Zn2+and Na+had no ef-fects on the stability of chlorophyl . Cu2+could enhance the stability of chlorophyl in solution.
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