高效液相色谱法测定食品中的爱德万甜

摘要

建立了食品中爱德万甜检测的高效液相色谱分析方法.样品经甲醇-水(50∶50,体积比)溶液提取10 min,离心后取上清液进行分析.采用Agilent TC-C18(2)(250 mm×4.6 mm,5μm)色谱柱分离,以甲醇-0.02 mol/L乙酸铵(53∶47,体积比)等度洗脱,在280 nm波长下检测,外标法定量.结果表明,目标化合物在1.0～200.0 mg/L范围内呈良好的线性,相关系数(r)为0.999 8,饮料、酸奶和蛋糕的检出限(S/N≥3)分别为0.6、1.5、9.0 mg/kg.方法的回收率为90.9％ ～ 103.4％,相对标准偏差(RSD,n=5)为1.1％～4.5％.该方法的色谱分离效果好,干扰少,准确度高,可用于实际样品中爱德万甜的测定.%A method for the determination of advantame in foods using high performance liquid chromatography(HPLC) was developed.The samples were extracted with methanol-water(50:50,by volume) in ultrasonic condition for 10 min.The supernatant was analyzed after centrifugation.Target compound was separated on an Agilent TC-C18 (2) (250 mm ×4.6 mm,5 μm) column at 280 nm,with methanol-0.02 mol/L ammonium acetate(53:47,by volume) as mobile phase by isocratic elution.The quantitative analysis was carried out by the external standard method.The results showed that the calibration curves were linear in the range of 1.0-200.0 mg/L with correlation coefficients of 0.999 8.The limits of detection(S/N≥3) of the method for the analytes in beverage,yoghourt and cake were 0.6,1.5 and 9.0 mg/kg,respectively.The average recoveries ranged from 90.9％ to 103.4％ with relative standard deviations(RSDs,n =5) of 1.1％-4.5％.With the advantages of good separation,few impurity disturbances and high accuracy,this method could be applied in the analysis of advantame in real samples.