不同壁材对乳化溶剂蒸发法制备南极磷虾油纳米乳的影响

摘要

采用乳清分离蛋白、酪蛋白酸钠、硬脂酸和玉米醇溶蛋白4种不同壁材,通过乳化溶剂蒸发法制备南极磷虾油纳米乳.对4种壁材制备的南极磷虾油纳米乳的包埋率、粒径、Zeta电位、形态、热稳定性及其脂肪酸组成与相对含量进行分析.结果表明,选用以酪蛋白酸钠为壁材制备的南极磷虾油纳米乳包埋率为(76.03±1.27)％,粒径为(141.92±3.38)nm,Zeta电位为(-22.65±3.32)mV;扫描电子显微镜分析显示纳米乳颗粒呈球状,表面光滑无褶皱;差示扫描量热分析显示纳米乳热熔温度较高,为(62.84±0.53)℃,在室温下热稳定性良好;与南极磷虾油相比,以酪蛋白酸钠为壁材制备的南极磷虾油纳米乳,包埋油中脂肪酸组成及相对含量基本没有变化.实验结果说明,以酪蛋白酸钠为壁材,采用乳化溶剂蒸发法制备的南极磷虾油纳米乳能较好地保留南极磷虾油本身良好的功能组分.%Four kinds of wall materials such as whey protein isolate ,sodium caseinate ,stearic acid and zein were used to prepare Antarctic krill oil nanoemulsion by emulsifying solvent evaporation method.The entrapment efficiency ,particle size ,Zeta potential ,morphology ,thermal stability ,the fatty acid composition and relative content of Antarctic krill oil nanoemulsion were analyzed.The results showed that Antarctic krill oil nanoemulsion prepared with sodium caseinate as wall material had the embedding rate of (76.03 ± 1.27)％ ,the particle size of (141.92 ± 3.38) nm and Zeta potential of (-22.65 ±3.32) mV.Scanning electron microscopy analysis showed that the nanoparticles in emulsion were spherical and smooth surface without wrinkles.Differential scanning calorimetry analysis showed that nanoemulsion had higher thermal dissolution temperature of (62.84 ± 0.53) ℃and good thermal stability at room temperature.Compared with Antarctic krill oil ,the fatty acid composition and relative content of Antarctic krill oil nanoemulsion remained no significant change.The above results indicated that the Antarctic krill oil nanoemulsion prepared by emulsifying solvent evaporation method using sodium caseinate as wall material could well maintain the functional properties of Antarctic krill oil.