蒲公英花中总酚酸和总黄酮含量测定及其抗氧化性能研究

摘要

In order to determine the contents of dandelion flowers,the material was extracted with 75% aqueous ethanol under the aid of ultrasonic.Meanwhile,the resultant ethanol extract was tested for its abilities to scavenge superoxide anion and hydroxyl free radicals and inhibit peanut oil autooxidation.The results indicated that the contents of total phenolic acid and total flavonoids in dandelion flowers were 4.09% and 3.65%,respectively.The ethanol extract from dandelion flowers could effectively scavenge superoxide anion and hydroxyl free radicals and inhibit peanut oil autooxidation in a dose-dependent manner.The half inhibitory concentrations against superoxide anion and hydroxyl free radicals were 96.1 μg/mL and 7.7μg/mL,respectively.The peroxide value（POV） of peanut oil showed a marked decline in the presence of the ethanol extract and the efficacy was similar to that of 0.10% vitamin C addition.%测定蒲公英花75%乙醇提取液中总酚酸和总黄酮含量,并测定该提取液对超氧阴离子自由基、羟自由基和油脂自氧化的清除作用。结果表明：蒲公英花中总酚酸平均含量为4.09%,总黄酮平均含量为3.65%。提取液对超氧阴离子自由基、羟自由基和油脂自氧化均有较好的清除效果,并在供试质量浓度范围内呈现量效关系。对超氧阴离子自由基的半数抑制率IC50为96.1μg/mL,对羟自由基的IC50为7.7μg/mL。蒲公英花提取液可使花生油的过氧化值（POV）显著降低,其效果与在花生油中添加0.10%的VC相当。