Trioside (25R)-spirost-5-en-3b,27-diol-27-O-(3-hydroxy-3-methylglutaroil), possessing antioxidant property The invention relates to a new biologically active substance from the steroid glycosides class, possessing antioxidant property and can be used in the cryobiology. The summary of the invention consists in obtaining of trioside (25R)-spirost-5-en-3b,27-diol-27-O-(3-hydroxy-3-methylglutaroil) of formula: CHFORM wherein Glc - glucoseRha - ramnose, possessing antioxidant property. The technical result of the invention consists in obtaining a new substance from the steroid glycosides class enabling to regulate the lipids peroxidation. Claims:1