三江镇腌菜中降解亚硝酸盐乳酸菌的筛选和初步鉴定

摘要

A strain of lactic acid bacteria with strong ability to degrade nitrite was screened from pickled potherb mustard made in Sanjiang town,Jiangxi province.The strongest ability of the strain to degrade nitrite averagely reached 32.66 μg/mL during 68 h of fermentation.The results of 16s rRNA sequencing showed that this strain was 98% homologous to Lactobacillus fermentum PL9005.Polygenetic analysis indicated that this strain belonged to Lactobacillus fermentum.%为筛选具有较强降解亚硝酸盐能力的乳酸菌,本实验经过初筛和复筛从三江镇雪里蕻腌菜中筛选出降解亚硝酸盐能力相对较强的乳酸菌菌株。筛选结果显示菌株对亚硝酸盐最强降解能力达到68h平均降解量32.66μg/mL。采用十六烷基三甲基溴化铵法（CTAB）法提取降解亚硝酸盐能力最强的乳酸菌11号菌株的基因组,测序后进行Blast同源性比较,其与Lactobacillus fermentum strain PL9005的同源性达到98%,并选取同源性较高的菌株构建系统发育进化树,初步鉴定该菌株为Lactobacillus fermentum。