新疆葡萄酒产区优良酒类酒球菌的分离、鉴定

摘要

In order to screen Oenococcus oeni with good performance for malolactic fermentation（MLF）,3 strains of O.oeni were isolated and purified from Xinjiang wines,and identified preliminarily as O.oeni based on morphological,physiological and biochemical analyses.Their resistance to individual stress conditions such as pH,ethanol or SO2 was determined.In addition,the growth of 9 screened strains under the combinatorial external stress of ethanol,pH and SO2 was also evaluated.The results indicated that 3 strains had better adaptation capability to adverse conditions.The species-specific PCR and homology analysis of 16S rRNA sequence also supported our results.Moreover,a phylogenetic tree was established based on the 16S rRNA sequence of O.oeni.The results of 16S rRNA sequencing showed that this strain was 99% homologous to O.oeni,so indicated that 9 screened strains with better performace was O.oeni species.%为了分离筛选出适合葡萄酒酿造的优良酒类酒球菌（Oenococcus oeni）菌株,从我国新疆葡萄酒产区分离纯化出30株酒类酒球菌,依据形态特征、生理生化特性,它们均被鉴定为酒类酒球菌。分别对其进行单因子（pH值、酒精、SO2）耐受性实验,选出单因子抗性较好的9株菌进行复合因子（pH值×酒精×SO2）耐受性实验,结果表明,有3株酒类酒球菌具有较好的发酵适应性。最后通过Species-specific PCR以及16S rRNA序列同源性分析对其进行验证,并构建相应的系统发育树,系统发育分析表明所筛菌株与酒类酒球菌的同源性均达到了99%以上,说明本实验所筛选的9株性能较好的菌株均为酒类酒球菌。