超声波协同酶解提取坛紫菜藻红蛋白工艺优化

摘要

The optimization of phycoerythrin extraction from Porphyra haitanensis were investigated by single experiments and response surface methodology.The single experiments were used to explore the effects on the extraction rate and purity of phycoerythrin by different enzyme amount,ultrasonic time,ultrasonic power,pH and solid-liquid ratio.The best pH and solid-liquid ratio were determined as 5.0 and 1 ∶ 40 (g/mL),respectively.Meanwhile,the enzyme amount,ultrasonic time and ultrasonic power were investigated through response surface experiment.The optimal extraction conditions were determined as follows:enzyme amount 0.05 g,ultrasonic time 40 min and ultrasonic power 90％ of the rated one.Under these conditions,the extraction rate and purity of phycoerythrin were reached (1.808±0.007) ％ and 0.460±0.001,respectively.%采用单因素试验和响应面法确定坛紫菜藻红蛋白提取的最佳工艺条件.通过单因素试验探讨加酶量、超声时间、超声功率、pH、固液比对藻红蛋白得率和纯度的影响,并根据单因素试验结果固定pH为5.0、固液比为1:40 (g/mL),同时选取加酶量、超声时间和超声功率为影响因素进行响应面优化试验,确定坛藻红蛋白的最优提取工艺参数为:加酶量0.05 g、超声时间40 min、超声功率为额定功率的90％(450 W).该条件下藻红蛋白的得率和纯度分别为(1.808±0.007)％和0.460±0.001.