藻红蛋白

摘要： 为了探究藻胆蛋白与高等植物类囊体膜之间的光能传递规律,验证重组藻胆蛋白是否可以与高等植物类囊体膜之间的光能传递,本研究分别提取了重组藻红蛋白、重组藻蓝蛋白以及菠菜和韭菜的类囊体进行混合孵育,然后进行了全波长吸收光谱以及荧光光谱检测.结果显示:混合孵育时间为10～20 m in有利于重组藻红蛋白和藻蓝蛋白与类囊体膜结合并进行光能传递,最适混合孵育时间随重组藻胆蛋白和类囊体的种类不同而稍有不同;重组藻红蛋白不能与菠菜或韭菜的类囊体膜直接发生光能传递,但是当体系中添加重组藻蓝蛋白的时候就可以;重组藻蓝蛋白可以与类囊体中的叶绿素a直接进行光能传递,使得叶绿素a的荧光发射峰值显著提升,当藻红蛋白和藻蓝蛋白的比例是1:2时,光能传递效率较高.本研究为藻胆蛋白在构建新型光合系统中的应用提供实验依据.

摘要： 本研究以普通机械搅拌式光照发酵罐为培养装置,采用单因素试验法对紫球藻生产藻红蛋白的条件(搅拌速率、光照强度、氮浓度以及NaCl浓度)进行优化.结果表明,发酵培养紫球藻高产藻红蛋白的最佳搅拌速率、光照强度、氮浓度以及NaCl浓度分别为150 r/min、2000 Lx、8 mmol/L、20‰.本试验优化了搅拌式光照发酵罐培养紫球藻生产藻红蛋白的条件,为紫球藻藻红蛋白的工业化生产提供基础数据及思路.

摘要： 以葛仙米为原料,研究葛仙米藻胆蛋白的超声辅助提取工艺.以藻胆蛋白提取率为评价指标,对提取工艺中葛仙米粉粒径数、超声功率、处理方法、液料比、提取时间、提取温度共6个因素进行单因素试验,并结合响应面分析对提取工艺进行优化.结果表明:采用超声辅助处理方法(超声功率700 W),当葛仙米粒径数120目~200目、液料比58:1(mL/g)、提取温度42°C、提取时间6 h时,葛仙米藻胆蛋白的提取率达到最大为26.13 %,达到预测值的99.54 %.采用一步离子交换树脂层析法纯化藻蓝蛋白(Phycocyanin,PC)和藻红蛋白(Phycoerythrin,PE),经聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis,SDS-PAGE)、凝胶过滤色谱(Gel filtration chromatography,GFC)、紫外可见吸收光谱(Ultraviolet-visible absorption spectroscopy,UV-Vis),藻蓝蛋白纯度达到93 %,藻红蛋白纯度83.5 %.%The ultrasonic-assisted extraction of phycobiliprotein from the raw material, nostoc sphaeroids kutzing,was investigated in this work.The single factor test investigated parameters of the extraction process included dry nostoc sphaeroids kutz powder size,ultrasonic irradiation power, treatment method, liquid-to-solid ratio, extraction time and extraction temperature, to evaluate the extraction rate of alga protein. The optimized extraction conditions were determined with the aid of the response surface analysis.Results indicated that the extraction yield of nostoc sphaeroids kutz phycobiliprotein was up to 26.13 %,reaching the predicted value of 99.54 %, under the optimized conditions, nostoc sphaeroids kutz powder size at 120 to 200 mesh, ultrasonic irradiation power 700 W, ultrasonic-assisted treatment, liquid-to-solid ratio at 58 : 1(mL/g), extraction temperature 42°C,and extraction time 6 h.Phycocyanin and phycoerythrin were purified by one-step ion exchange resin chromatography.By sodium dodecyl sulfate polyacrylamide gel electrophoresis,Gel filtration chromatography and Ultraviolet-visible absorption spectroscopy, the purities of the purified PC and PE were 93 % and 83.5 %,respectively.

摘要： 用戊二醛和EDC/sulfo-NHS分别活化表面修饰了氨基和羧基的微球,在2种微球上分别固定相等摩尔浓度的氨基标记的发卡DNA和未经修饰的人TNF α捕获抗体,结果表明戊二醛的交联效果优于EDC/sulfo-NHS.从而选择戊二醛为交联剂将Cy3标记的发卡DNA和未经修饰的藻红蛋白(RPE)分别固定到微米球表面并进行了荧光观察,再通过调整蛋白和DNA的比例将FAM标记的发卡DNA和RPE同时固定到微米球上并观察荧光,为实现用DNA序列编码的微米球上多种生物标志物酶联免疫吸附检测奠定了基础.用RPE为荧光标记物采用双抗体夹心法在微球上进行荧光免疫反应,为商品化诊断试剂的研究提供一定的参考.

摘要： 利用响应面分析法优化藻红蛋白的提取工艺.在单因素实验基础上,使用硫酸铵分级沉淀提取纯化藻红蛋白,选超声时间、温度、功率为影响因素,以藻红蛋白得率为响应值,根据Box-Behnken实验设计原理对藻红蛋白提取工艺进行响应面分析,优化藻红蛋白提取工艺.结果表明:在超声时间为16 min、温度为33°C、功率为750W时藻红蛋白得率取得最大值.在此优化条件下进行验证性实验,测得蛋白得率为15.93％,与预测值相对误差为2.50％,表明该优化工艺具有良好的可行性.藻红蛋白对O2-·和ABTS+·具有一定的清除作用,其IC50分别为0.025 mg/mL和0.023 mg/mL.

摘要： The optimization of phycoerythrin extraction from Porphyra haitanensis were investigated by single experiments and response surface methodology.The single experiments were used to explore the effects on the extraction rate and purity of phycoerythrin by different enzyme amount,ultrasonic time,ultrasonic power,pH and solid-liquid ratio.The best pH and solid-liquid ratio were determined as 5.0 and 1 ∶ 40 (g/mL),respectively.Meanwhile,the enzyme amount,ultrasonic time and ultrasonic power were investigated through response surface experiment.The optimal extraction conditions were determined as follows:enzyme amount 0.05 g,ultrasonic time 40 min and ultrasonic power 90％ of the rated one.Under these conditions,the extraction rate and purity of phycoerythrin were reached (1.808±0.007) ％ and 0.460±0.001,respectively.%采用单因素试验和响应面法确定坛紫菜藻红蛋白提取的最佳工艺条件.通过单因素试验探讨加酶量、超声时间、超声功率、pH、固液比对藻红蛋白得率和纯度的影响,并根据单因素试验结果固定pH为5.0、固液比为1:40 (g/mL),同时选取加酶量、超声时间和超声功率为影响因素进行响应面优化试验,确定坛藻红蛋白的最优提取工艺参数为:加酶量0.05 g、超声时间40 min、超声功率为额定功率的90％(450 W).该条件下藻红蛋白的得率和纯度分别为(1.808±0.007)％和0.460±0.001.

摘要： 大型海藻龙须菜富含活性多糖、藻红蛋白、膳食纤维和营养元素等,具有调节免疫活性、抗肿瘤、抗氧化、抗病毒等多种生物活性、药用价值和保健功效,经常食用可预防肥胖、高胆固醇、高血脂、便秘等代谢疾病.近年来,随着龙须菜抗肿瘤活性成分的筛选与深入分析,越来越多的人开始关注龙须菜的抗肿瘤活性及其在肿瘤防治中的作用与机理.对龙须菜药用价值及其抗肿瘤活性的新进展进行总结有利于指导抗肿瘤新药的研制和开发,从而更好地预防和治疗肿瘤.

摘要： 台湾锯齿藻，属于红藻门真红藻纲隐丝藻目海膜科锯齿藻属，地理分布为中国的台湾和海南、日本、越南、菲律宾，藻体紫红色，软骨质，含有丰富的藻红蛋白。台湾锯齿藻在国内外很少有人研究，本研究对台湾锯齿藻的藻红蛋白进行了分离纯化并对其基本特性进行了初步研究，为该类蛋白的应用做铺垫。研究阐明：台湾锯齿藻作为一种未被广泛关注的红藻，可以从中提取出高纯度的藻红蛋白。而且，提取方法较为简便：细胞破碎、盐析、脱盐、层析。荧光光谱分析说明这种PE较适用于免疫荧光标记检测和肿瘤光动力学治疗，它能避开组织细胞自发荧光的干扰，达到最好的疾病诊断效果；激发光波长较宽，利于光源选择；能特异性地聚集在肿瘤细胞周围，且发射光谱较为集中，荧光量子产率极高，杀伤肿瘤细胞的效率非常高；且其藻红蛋白为天然产物，光敏副作用小。这些特性赋予了藻红蛋白在医学研究及疾病治疗方面广阔的应用前景。

摘要： 藻红蛋白的药理作用越来越受到国内外科研人员的关注。科研工作者己对藻红蛋白的药理作用进行了系统的研究，本论文对其药理活性作用的主要几点逐一说明，有助于深入探究藻红蛋白药理作用机制，能更好的应用藻红蛋白这种高活性的海洋药物。

摘要： 目的：研究藻红蛋白(PE)对人乳腺癌MCF-7细胞的增殖抑制效果和诱导凋亡作用。方法：采用MTT法和SRB法检测PE对人乳腺癌MCF-7细胞增殖的抑制作用。采用倒置显微镜、荧光显微镜Hoechst 33258染色观察MCF-7细胞凋亡细胞形态。Annexin V-FITC/PI双荧光染色观察细胞凋亡过程中特异的磷脂酰丝氨酸(PS)外翻情况确定凋亡的出现。流式细胞仪观察PE对MCF-7细胞周期的影响。结果： PE对MCF-7细胞有较强的生长抑制作用,并且呈一定的剌量依赖性,其72h的IC50值为147.82μg/mL,GI50值为109.76μg/mL。倒置显微镜下与基本贴壁的饱满、边沿清晰的对照组细胞比较,给药组细胞有明显的细胞变粗糙,贴壁细胞减少的现象,而且浓度越高。作用时间越长,悬浮细胞越多。荧光显微镜下可见各给药组MCF-7细胞出现不同程度深染亮点的细胞核质,染色质浓集固缩并伴有凋亡小体形成。激光共聚焦显微镜下可见给药组细胞膜被染成绿色,细胞核被染成红色。流式细胞仪显示PE可阻滞MCF-7细胞从S期进入G2/M期,促使细胞凋亡。结论：PE对MCF-7细胞有较强的抑制作用,可使其凋亡,其作用机制与周期阻滞有关。

摘要： 藻红蛋白(phycoerythrin,PE)是许多海藻的重要捕光色素蛋白,其与藻蓝蛋白、异藻蓝蛋白等藻胆蛋白组成棒状的藻胆体.藻红蛋白主要分布在海藻的红藻中,是由脱辅基寡聚蛋白与开链的四吡咯发色团共价结合而成,组成寡聚蛋白的亚基有α、β、γ等,通常红藻中的藻红蛋白是以(αβ)6γ形式存在,结合在亚基半胱氨酸残基上的色素分子有藻红胆素(PEB)和藻尿胆素(PUB),由于色素分子的存在,藻红蛋白在可见光区480～570 nm波段有较强的吸收,且可产生强烈的荧光.藻红蛋白可以作为天然色素应用于食品、化妆品、染料等行业,还可制成荧光试剂,用于临床医学诊断和免疫化学及生物工程等研究领域.藻红蛋白还是一种重要的生理活性物质,可制成光敏剂应用于肿瘤光动力学治疗.目前,国内外对藻红蛋白的纯化和性质等研究主要集中在紫球藻、多管藻、珊瑚藻等红藻上,而对红毛藻藻红蛋白的研究并不多.本文对红毛藻中藻红蛋白的分离纯化方法及性质作了研究。

摘要： R-phycoerythrin, which is a light-harvesting protein in some of marine algae, was isolated and purified from red alga, Gracilaria verrucosa, using the STREAMLINE column (expanded bed adsorption) combined with an ion-exchange chromatography. Because the crude extract was applied to the column with an upward direction, the expanded bed adsorption column can effectively overcome the blockage of a general chromatography column from rich polysaccharides in extract of marine algae. Followed by applying the crude extract containing 0.5 mol/M (NH4)2SO4, some different concentration of (NH4)2SO4 solution (0.2 mol/L, 0.1 mol/L and 0.05 mol/L) was used to apply to the column with a reverse direction (in a downward direction) and 0.1 mol/L (NH4)2SO4 was proved best to elute R-phycoerythrin from the STREAMLINE column. The desalted eluate with 0.1 mol/L (NH4)2SO4 was applied into a ion-exchange chromatography column loaded with Q-sepharose to continue to purify the R-phycoerythrin. Through the two steps for isolation and purification, purity ( OD565/OD280) of the R-phycoerythrin from Gracilaria verrucosa is up to 4.4 which is much more than 3.2, a criterion for purity, and the yield of the purified R-phycoerythrin can reach 0.141 mg per gram of frozen Gracilaria verrucosa, which is much higher than the yield of phycobiliproteins purified with the already known methods. The result indicates that the price of R-phycoerythrin will be dropped down if using the method reported in this article.

摘要： R-phycoerythrin, which is a light-harvesting protein in some of marine algae, was isolated and purified from red alga, Gracilaria verrucosa, using the STREAMLINE column (expanded bed adsorption) combined with an ion-exchange chromatography. Because the crude extract was applied to the column with an upward direction, the expanded bed adsorption column can effectively overcome the blockage of a general chromatography column from rich polysaccharides in extract of marine algae. Followed by applying the crude extract containing 0.5 mol/M (NH4)2SO4, some different concentration of (NH4)2SO4 solution (0.2 mol/L, 0.1 mol/L and 0.05 mol/L) was used to apply to the column with a reverse direction (in a downward direction) and 0.1 mol/L (NH4)2SO4 was proved best to elute R-phycoerythrin from the STREAMLINE column. The desalted eluate with 0.1 mol/L (NH4)2SO4 was applied into a ion-exchange chromatography column loaded with Q-sepharose to continue to purify the R-phycoerythrin. Through the two steps for isolation and purification, purity ( OD565/OD280) of the R-phycoerythrin from Gracilaria verrucosa is up to 4.4 which is much more than 3.2, a criterion for purity, and the yield of the purified R-phycoerythrin can reach 0.141 mg per gram of frozen Gracilaria verrucosa, which is much higher than the yield of phycobiliproteins purified with the already known methods. The result indicates that the price of R-phycoerythrin will be dropped down if using the method reported in this article.

摘要： R-phycoerythrin, which is a light-harvesting protein in some of marine algae, was isolated and purified from red alga, Gracilaria verrucosa, using the STREAMLINE column (expanded bed adsorption) combined with an ion-exchange chromatography. Because the crude extract was applied to the column with an upward direction, the expanded bed adsorption column can effectively overcome the blockage of a general chromatography column from rich polysaccharides in extract of marine algae. Followed by applying the crude extract containing 0.5 mol/M (NH4)2SO4, some different concentration of (NH4)2SO4 solution (0.2 mol/L, 0.1 mol/L and 0.05 mol/L) was used to apply to the column with a reverse direction (in a downward direction) and 0.1 mol/L (NH4)2SO4 was proved best to elute R-phycoerythrin from the STREAMLINE column. The desalted eluate with 0.1 mol/L (NH4)2SO4 was applied into a ion-exchange chromatography column loaded with Q-sepharose to continue to purify the R-phycoerythrin. Through the two steps for isolation and purification, purity ( OD565/OD280) of the R-phycoerythrin from Gracilaria verrucosa is up to 4.4 which is much more than 3.2, a criterion for purity, and the yield of the purified R-phycoerythrin can reach 0.141 mg per gram of frozen Gracilaria verrucosa, which is much higher than the yield of phycobiliproteins purified with the already known methods. The result indicates that the price of R-phycoerythrin will be dropped down if using the method reported in this article.

摘要： The mouse tumor cell S180 and human liver carcinomacell SMC 7721 cells were first treated with R-PE andits subunits (a,b,gsubunits), then irradiated withArgon laser (496nm,28.8J/cm2). Survival rate wasmeasured by MTT method. In order to compare thephototoxicity in normalcells, the mouse marrow cells were treated withphotofrin II and b-subunit, irradiated with 45 J/cm2of light; survival rate was also measured by MTT method.

摘要： The mouse tumor cell S180 and human liver carcinomacell SMC 7721 cells were first treated with R-PE andits subunits (a,b,gsubunits), then irradiated withArgon laser (496nm,28.8J/cm2). Survival rate wasmeasured by MTT method. In order to compare thephototoxicity in normalcells, the mouse marrow cells were treated withphotofrin II and b-subunit, irradiated with 45 J/cm2of light; survival rate was also measured by MTT method.

摘要： The mouse tumor cell S180 and human liver carcinomacell SMC 7721 cells were first treated with R-PE andits subunits (a,b,gsubunits), then irradiated withArgon laser (496nm,28.8J/cm2). Survival rate wasmeasured by MTT method. In order to compare thephototoxicity in normalcells, the mouse marrow cells were treated withphotofrin II and b-subunit, irradiated with 45 J/cm2of light; survival rate was also measured by MTT method.

摘要： 本发明公开了一种葛仙米藻红蛋白的纯化方法，包括：(1)选用阴离子交换柱过柱；(2)配置PB缓冲液Buffer A和PB‑NaCl缓冲液Buffer B；(3)葛仙米藻胆蛋白粗提粉末用PB缓冲液Buffer A溶解，离心，过滤；(4)洗泵，用PB缓冲液Buffer A平衡离子交换柱；(5)将步骤(3)制备的溶解于PB缓冲液Buffer A中的葛仙米藻胆蛋白直接上样，PB‑NaCl缓冲液Buffer B梯度洗脱，收集洗脱液；(6)用盐溶液清洗离子交换柱，然后用碱溶液反向冲洗；(7)对淋洗液进行超滤浓缩，透析，得到纯化的葛仙米藻红蛋白水溶液。本发明的方法操作简单，得到的葛仙米藻红蛋白浓度和纯度都得到显著提高，适于工业化大规模生产。

摘要： 一种快速分离纯化R-藻红蛋白、R-藻蓝蛋白的方法，属于红藻分离纯化技术领域。本发明以红藻为材料，应用经冻溶和低离子强度溶涨提取R-藻红蛋白、R-藻蓝蛋白，经硫酸铵沉淀进行初步纯化，然后使用阴离子交换层析吸附富集R-藻红蛋白、R-藻蓝蛋白，并通过一步阴离子交换层析分步洗脱大量制备得到高纯度的R-藻红蛋白、R-藻蓝蛋白。本方法省去了传统的应用分子筛层析结合羟基磷灰石层析多步层析的复杂分离纯化程序，克服了难于规模化快速制备的难题，操作简便，省时省力，对设备要求简单，得率高。

摘要： 本发明涉及一种藻红蛋白与壳寡糖共价交联来提高藻红蛋白稳定性的方法，步骤如下：步骤一：制备干燥坛紫菜的藻红蛋白粗提液；步骤二：配制藻红蛋白溶液，向藻红蛋白溶液中加入转谷氨酰胺酶及壳寡糖进行酶促反应，得到粗制的藻红蛋白‑壳寡糖复合物；步骤三：反应结束后，将上述复合物经灭酶，然后立即冷却至室温后，透析除去未结合的壳寡糖，得到藻红蛋白‑壳寡糖复合物。该复合物相比于藻红蛋白表现出了较高的光、热、pH稳定性。本发明方法利用转谷氨酰胺酶催化藻红蛋白与壳寡糖发生反应来提高藻红蛋白稳定性，从而增加藻红蛋白的利用率和使用范围，本发明方法尤其适用于用天然植物蛋白与多糖类结合从而提高色素稳定性。

摘要： 本发明公开了一种藻红蛋白(PE)与Annexin V蛋白偶联方法，包括以下步骤：步骤1：Annexin V蛋白表达纯化：1.1：基因序列优化：查找人Annexin V蛋白的基因序列，将人Annexin V基因序列优化，去除终止密码子序列，将其连入原核表达载体pET28a上，保留前后His标签蛋白，为蛋白纯化及后续偶联荧光素纯化做准备；1.2：Annexin V蛋白诱导表达：挑取pET28a‑Annexin V质粒单菌落，接种到10ml含有50μg/ml卡那霉素的LB液体培养基中，37°C200rpm振荡培养过夜，按照1：100将培养的菌液接种到含50μg/ml卡那霉素的LB液体培养基中，37°C200rpm振荡培养至菌液浓度OD600为04‑0.6；本发明先通过分子筛纯化去除大部分游离的PE后再采用镍柱纯化浓缩，得到纯度较高的Annexin V‑PE标记物，且保持蛋白良好的生物活性，可用于细胞凋亡检测。

摘要： 本发明提供一种藻红蛋白荧光标记蛋白的方法，该方法包括如下步骤：步骤一、对藻红蛋白上的游离巯基作封闭处理；步骤二、1)将目标蛋白巯基化；2)采用SMCC对步骤1)获得的藻红蛋白进行活化；3)将巯基化后的目标蛋白与活化后的藻红蛋白交联；先对藻红蛋白上的游离巯基作封闭处理，在藻红蛋白上的游离巯基被封闭后，后续的胺‑巯基交联剂只能与被封闭处理藻红蛋白上的氨基反应，而不会与相应蛋白上的巯基反应，如此能够确保胺‑巯基交联剂的氨基全部用于偶联被巯基化的藻红蛋白或目标蛋白，获得的藻红蛋白免疫荧光探针在进行免疫检测时，也能获得较强阳性信号，大大提高信噪比。

摘要： 本发明属于便携式海水原位荧光探测技术领域，公开了海水叶绿素a、藻蓝蛋白和藻红蛋白参数测量装置及方法，包括微型压电泵、流通池、激发光源、透镜、分光器、过滤片和光电二极管，激发光源可照射流通池。本技术方案采用微流控芯片技术，将预处理，反应，分离和监测等单元集成到芯片内，在芯片上快速完成海水叶绿素a、藻蓝蛋白、藻红蛋白浓度的测量，自动进样、分析、校正，自动化程度高，效率高，仪器体积减小优化，系统集成度高，功耗低，成本低，适合原位测量、快速测量、连续测量、实验室测量和便携野外测量。

摘要： 本发明公开了一种葛仙米藻红蛋白的纯化方法，包括：(1)选用阴离子交换柱过柱；(2)配置PB缓冲液Buffer A和PB‑NaCl缓冲液Buffer B；(3)葛仙米藻胆蛋白粗提粉末用PB缓冲液Buffer A溶解，离心，过滤；(4)洗泵，用PB缓冲液Buffer A平衡离子交换柱；(5)将步骤(3)制备的溶解于PB缓冲液Buffer A中的葛仙米藻胆蛋白直接上样，PB‑NaCl缓冲液Buffer B梯度洗脱，收集洗脱液；(6)用盐溶液清洗离子交换柱，然后用碱溶液反向冲洗；(7)对淋洗液进行超滤浓缩，透析，得到纯化的葛仙米藻红蛋白水溶液。本发明的方法操作简单，得到的葛仙米藻红蛋白浓度和纯度都得到显著提高，适于工业化大规模生产。

摘要： 一种快速分离纯化R－藻红蛋白、R－藻蓝蛋白的方法，属于红藻分离纯化技术领域。本发明以红藻为材料，应用经冻溶和低离子强度溶涨提取R－藻红蛋白、R－藻蓝蛋白，经硫酸铵沉淀进行初步纯化，然后使用阴离子交换层析吸附富集R－藻红蛋白、R－藻蓝蛋白，并通过一步阴离子交换层析分步洗脱大量制备得到高纯度的R－藻红蛋白、R－藻蓝蛋白。本方法省去了传统的应用分子筛层析结合羟基磷灰石层析多步层析的复杂分离纯化程序，克服了难于规模化快速制备的难题，操作简便，省时省力，对设备要求简单，得率高。

摘要： 本发明公开了一种荧光藻蓝蛋白与荧光藻红蛋白，该藻蓝蛋白与藻红蛋白的分子结构为通过硫醚键连接的载体蛋白与发色团－－开链线性延展的四吡咯化合物，是从藻类中提取纯化的，在适宜波长激发下能发射强烈荧光。本发明还公开了一系列该荧光藻蓝蛋白与荧光藻红蛋白的用途，包括在荧光检测及免疫分析方面的用途，在制备抗癌抗肿瘤药物或食物方面的用途，在制备抗辐射增强免疫力消炎药物或食物方面的用途，在制备光子开关方面的用途，以及在制备食品或化妆品添加剂方面的用途。

摘要： 本实用新型属于便携式海水原位荧光探测技术领域，公开了海水叶绿素a、藻蓝蛋白和藻红蛋白参数测量装置及方法，包括微型压电泵、流通池、激发光源、透镜、分光器、过滤片和光电二极管，激发光源可激发流通池。本技术方案采用微流控芯片技术，将预处理，反应，分离和监测等单元集成到芯片内，在芯片上快速完成海水叶绿素a、藻蓝蛋白、藻红蛋白浓度的测量，自动进样、分析、校正，自动化程度高，效率高，仪器体积减小优化，系统集成度高，功耗低，成本低，适合原位测量、快速测量、连续测量、实验室测量和便携野外测量。