HPLC-DAD法同时测定麦味地黄丸中6种成分

摘要

目的 建立HPLC-DAD法同时测定麦味地黄丸(麦冬、五味子、熟地黄等)中6种成分的含有量.方法 该药物50％甲醇提取液的分析采用Agilent ZORBAX SB-C18色谱柱(4.6 mm×150 mm,5μm);流动相乙腈-水(含0.2％磷酸),梯度洗脱;体积流量0.8 mL/min;柱温35℃;检测波长220、230、236、274 nm.结果 五味子甲素、五味子乙素、五味子醇甲、芍药苷、丹皮酚、马钱苷分别在10～ 70、6.5 ～45.5、33.5～ 234.5、17 ～ 119、31 ～ 217、34～238 μg/mL范围内线性关系良好(r＞0.990 0),平均加样回收率(RSD)分别为99.6％(1.7％)、100.4％(1.8％)、100.7％(1.8％)、102.9％(1.7％)、102.2％(1.5％)、99.7％(1.2％).结论 该方法简单、快速、重复性好,可用于麦味地黄丸的质量控制.%AIM To establish an HPLC-DAD method for the simultaneous content determination of six constituents in Maiwei Dihuang Pills (Ophiopogonis Radix,Schisandrae chinensis Fructus,Rehmanniae Radix Praeparata,etc.).METHODS The analysis of 50％ methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent ZORBAX SB-C18column (4.6 mm × 150 mm,5 μm),with the mobile phase comprising of acetonitrile-water (containing 0.2％ phosphate acid) flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelengths were set at 220,230,236 and 274 nm.RESULTS Deoxyschizandrin,schizandrin B,schisandrin,paeoniflorin,paeonol and loganin showed good linear relationships within the ranges of 10-70,6.5-45.5,33.5-234.5,17-119,31-217 and 34-238 μg/mL (r ＞0.990 0),whose average recoveries (RSDs) were 99.6％ (1.7％),100.4％ (1.8％),100.7％ (1.8％),102.9％ (1.7％),102.2％ (1.5％) and 99.7％ (1.2％),respectively.CONCLUSION This simple and reproducible method can be used for the rapid quality control of Maiwei Dihuang Pills.