以新疆红枣为原料,为优化新疆红枣中总黄酮的乙醇提取工艺,选择提取温度、乙醇浓度、液料比为自变量,总黄酮含量作为响应值,采用Box-Behnken设计方法,研究各自变量及其交互作用对总黄酮提取量的影响.采用响应面分析软件,模拟得到二次多项式回归方程的预测模型,并确定乙醇提取最佳工艺条件为提取温度66℃、乙醇浓度60％vol、液料比10.0.在此条件下,总黄酮的平均提取量为2.32mg/g,与理论预测值基本吻合,说明响应面法优化新疆红枣总黄酮乙醇提取工艺可行.%To optimize the ethanol extraction of total flavonoids from Xinjiang jujube, the effects of extraction temperature, ethanol concentration, liquid to solid ratio and their interactions on the extraction yield of jujube flavonoids were studied with Box-Behnken design. The predictive polynomial quadratic equations model was developed by response surface methodology software. The optimum extraction conditions were obtained as follows: extraction temperature 66℃, ethanol concentration 60%vol and liquid to solid ratio 10.0. Under the optimal conditions, the extraction rate of total flavonoids was 2.32mg/g, which agreed well with the predictive value, indicating the optimized process was feasible.
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